Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice till...Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice tiller buds.To further elucidate the molecular mechanism of MOC1 involved in the regulation of rice tillering,we performed a yeast-two-hybrid screening to identify MOC1 interacting proteins （MIPs）.Here we reported that MIP1 interacted with MOC1 both in vitro and in vivo.The overexpression of MIP1 resulted in enhanced tillering and reduced plant height.In-depth characterization of the context of MIP1 and MOC1 would further our understanding of molecular regulatory mechanisms of rice tillering.展开更多
目的探讨巨噬细胞炎症蛋白⁃1α(MIP⁃1α)拮抗剂联合硼替佐米通过抑制Erk1/2/Bax促进骨髓瘤骨病(myeloma bone disease,MBD)成骨作用。方法采用皮下注射骨髓瘤细胞株方法构建MBD小鼠模型,按药物给予分为对照组、硼替佐米(Bor)组、Bx471(M...目的探讨巨噬细胞炎症蛋白⁃1α(MIP⁃1α)拮抗剂联合硼替佐米通过抑制Erk1/2/Bax促进骨髓瘤骨病(myeloma bone disease,MBD)成骨作用。方法采用皮下注射骨髓瘤细胞株方法构建MBD小鼠模型,按药物给予分为对照组、硼替佐米(Bor)组、Bx471(MIP⁃1α拮抗剂)组、硼替佐米(Bor)联合Bx471(MIP⁃1α拮抗剂)组;ELISA检测MIP⁃1α、SOST、IL⁃6、BALP、RANKL、OPG水平,Western blot检测Erk、Bax、Caspase 3、Caspase 9表达水平,X线评估骨质破坏程度。结果X线显示荷瘤小鼠(对照组)出现病理性骨折,Bor组、Bx471组及Bor联合Bx471组骨质破坏程度低于对照组。对照组的MIP⁃1α、SOST、RANKL、IL⁃6水平高于Bor组及Bx471组(P<0.05),明显高于Bor联合Bx471组(P<0.001),而OPG、BALP的表达明显低于Bor联合Bx471组(P<0.001)。对照组的Erkl/2、Bax、Caspase 3、Caspase 9蛋白表达高于Bor组及Bx471组(P<0.05),且明显高于Bor联合Bx471组(P<0.001)。结论MIP⁃1ɑ拮抗剂联合硼替佐米可能抑制Erk1/2/Bax信号通路促进MBD成骨作用。展开更多
Tomato mottle mosaic virus(ToMMV)poses a threat to production and quality of tomato fruits.The Tm-2^(2)gene confers resistance to some tobamoviruses by recognizing viral movement proteins.However,Tm-2^(2)-mediated res...Tomato mottle mosaic virus(ToMMV)poses a threat to production and quality of tomato fruits.The Tm-2^(2)gene confers resistance to some tobamoviruses by recognizing viral movement proteins.However,Tm-2^(2)-mediated resistance against ToMMV is not well known.Here,we found that ToMMV could infect wild-type but not Tm-2^(2)transgenic Nicotiana benthamiana plants and could also infect tomato cultivar Moneymaker but not resistant cultivar Jili with homozygous Tm-2^(2).Chimeric viral ToMMVToBRFV−MP with swapped ToMMV MP to MP of tomato brown rugose fruit virus could systemically infect Tm-2^(2)transgenic N.benthamiana and tomato cultivars Jili plants.Further,transient expression of ToMMV MP in the leaves of Tm-2^(2)transgenic N.benthamiana plants induced hypersensitive response-associated cell death,suggesting that the MP of ToMMV was the avirulent factor for the Tm-2^(2)resistance gene.ToMMV could infect Tm-2^(2)-containing cultivar Jinpeng 1 but not Chaobei.Sequence analysis revealed that cultivars Chaobei and Jinpeng 1 were heterozygous,where Chaobei consists of Tm-2^(2)and Tm-2 genes,while Jinpeng 1 consists of Tm-2^(2)and tm-2 genes.Transient co-expression assays showed that both Tm-2^(2)and Tm-2 but not tm-2 could recognize ToMMV MP and induce hypersensitivity response-associated cell death in N.benthamiana leaves,suggesting that homozygous tomato harboring Tm-2^(2)and heterozygous tomato containing Tm-2^(2)and Tm-2 may exhibit more durable resistance to ToMMV than heterozygous tomato carrying Tm-2^(2)and tm-2.Further,Tm-2^(2)transgenic N.benthamiana and tomato cultivar Jili plants with silenced Tm-2^(2)gene were susceptible to ToMMV.Also,silencing type-I J-domain MIP1 gene compromised Tm-2^(2)-mediated resistance to ToMMV in Tm-2^(2)transgenic N.benthamiana and tomato cultivar Jili.Moreover,we found that viral RNA could accumulate in the systemic leaves of Tm-2^(2)transgenic N.benthamiana plants and tomato cultivar Jili at 35°C,but not at 20,25,or 30°C.Altogether,our findings reveal that the Tm-2^(2)confers resistance to ToMMV by recognizing MP,and the resistance is regulated by the allele combinations,accumulation levels of Tm-2^(2),MIP1,and the temperature.展开更多
基金supported by the grant from the Ministry of Science and Technology of China (No. 2005CB1208)
文摘Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice tiller buds.To further elucidate the molecular mechanism of MOC1 involved in the regulation of rice tillering,we performed a yeast-two-hybrid screening to identify MOC1 interacting proteins （MIPs）.Here we reported that MIP1 interacted with MOC1 both in vitro and in vivo.The overexpression of MIP1 resulted in enhanced tillering and reduced plant height.In-depth characterization of the context of MIP1 and MOC1 would further our understanding of molecular regulatory mechanisms of rice tillering.
基金supported by grants from the National Natural Science Foundation of China(32072387)the‘Taishan Scholar’Construction Project,China(TS201712023).
文摘Tomato mottle mosaic virus(ToMMV)poses a threat to production and quality of tomato fruits.The Tm-2^(2)gene confers resistance to some tobamoviruses by recognizing viral movement proteins.However,Tm-2^(2)-mediated resistance against ToMMV is not well known.Here,we found that ToMMV could infect wild-type but not Tm-2^(2)transgenic Nicotiana benthamiana plants and could also infect tomato cultivar Moneymaker but not resistant cultivar Jili with homozygous Tm-2^(2).Chimeric viral ToMMVToBRFV−MP with swapped ToMMV MP to MP of tomato brown rugose fruit virus could systemically infect Tm-2^(2)transgenic N.benthamiana and tomato cultivars Jili plants.Further,transient expression of ToMMV MP in the leaves of Tm-2^(2)transgenic N.benthamiana plants induced hypersensitive response-associated cell death,suggesting that the MP of ToMMV was the avirulent factor for the Tm-2^(2)resistance gene.ToMMV could infect Tm-2^(2)-containing cultivar Jinpeng 1 but not Chaobei.Sequence analysis revealed that cultivars Chaobei and Jinpeng 1 were heterozygous,where Chaobei consists of Tm-2^(2)and Tm-2 genes,while Jinpeng 1 consists of Tm-2^(2)and tm-2 genes.Transient co-expression assays showed that both Tm-2^(2)and Tm-2 but not tm-2 could recognize ToMMV MP and induce hypersensitivity response-associated cell death in N.benthamiana leaves,suggesting that homozygous tomato harboring Tm-2^(2)and heterozygous tomato containing Tm-2^(2)and Tm-2 may exhibit more durable resistance to ToMMV than heterozygous tomato carrying Tm-2^(2)and tm-2.Further,Tm-2^(2)transgenic N.benthamiana and tomato cultivar Jili plants with silenced Tm-2^(2)gene were susceptible to ToMMV.Also,silencing type-I J-domain MIP1 gene compromised Tm-2^(2)-mediated resistance to ToMMV in Tm-2^(2)transgenic N.benthamiana and tomato cultivar Jili.Moreover,we found that viral RNA could accumulate in the systemic leaves of Tm-2^(2)transgenic N.benthamiana plants and tomato cultivar Jili at 35°C,but not at 20,25,or 30°C.Altogether,our findings reveal that the Tm-2^(2)confers resistance to ToMMV by recognizing MP,and the resistance is regulated by the allele combinations,accumulation levels of Tm-2^(2),MIP1,and the temperature.
