目的:建立荧光标记D1S1676、D2S441、D3S4529、D22S1045、amelogenin 5个mini短串联重复序列(Short tandem repeat,STR)基因座复合扩增体系,并对重庆地区汉族200名无关个体此5个基因座的遗传多态性进行研究分析。方法:分别用5’TAMRA标...目的:建立荧光标记D1S1676、D2S441、D3S4529、D22S1045、amelogenin 5个mini短串联重复序列(Short tandem repeat,STR)基因座复合扩增体系,并对重庆地区汉族200名无关个体此5个基因座的遗传多态性进行研究分析。方法:分别用5’TAMRA标记D1S1676引物,5’6-FAM标记D2S441和D3S4529引物,5’HEX标记D22S1045引物,5’TET标记amelogenin引物,构建及优化符合扩增体系,运用3130基因分析仪检测并收集电泳结果数据,通过Gene Mapper v3.2.1软件计算扩增产物片段相对大小以及进行样本基因型分型。结果:5个miniSTR基因座荧光标记复合扩增体系每个位点都获得清晰的分型结果。对重庆地区汉族无关个体200份血样进行分型,5个基因座分别检出9、9、8、9、2个等位基因和103种基因型,其基因型分布均符Hardy-Weinberg平衡。其中D1S1676、D2S441、D3S4529、D22S1045基因座在重庆汉族群体的杂合度(Heterozygosity,Ho)分别依次为0.710、0.765、0.730、0.710;多态信息含量(Polymorphism information content,PIC)分别依次为:0.660 041、0.798 329、0.753 177、0.797 888;累计非父排除率(Exclusion probability of paternity,EP)为0.924 800 4,累计个人识别率为0.999 955 1。结论:荧光标记D1S1676、D2S441、D3S4529、D22S1045、amelogenin 5个miniSTR基因座复合扩增体系,每个基因座可获得准确分型;重庆汉族群体该5个基因座的遗传学数据,可作为群体遗传学和法医学研究与应用的基础资料。展开更多
A Malian governors’ delegation led by Gen. Kafougouna Kone, minister of territorial administration and local communities, visited Beijing, Yunnan and Guangdong in early March at the invitation of the CPAFFC.
五菱宏光MINI EV是上汽通用五菱汽车股份有限公司在充分考虑市场需求的情况下推出了一款新能源汽车,它上市初期连续19个月在中国新能源市场销量排名第一,然而,随着政府政策以及市场的变化,其竞争力也有所下降。本文综合运用案例分析和...五菱宏光MINI EV是上汽通用五菱汽车股份有限公司在充分考虑市场需求的情况下推出了一款新能源汽车,它上市初期连续19个月在中国新能源市场销量排名第一,然而,随着政府政策以及市场的变化,其竞争力也有所下降。本文综合运用案例分析和文献研究法等方法,对新能源汽车宏光MINI EV的营销策略进行了研究。首先,借助STP和4P理论研究分析了宏光MINI EV的营销现状,发现了其在营销方面存在产品核心竞争力不足,低利润难以维持低价营销策略,促销活动较盲目和低效,对市场定位过于局限以及数字营销相对滞后等问题。然后,为宏光MINI EV的营销提供了优化建议,包括在产品方面,改良产品,提升产品竞争力;推动技术升级等。在价格方面,采取差异化定价等。在渠道方面,推动全渠道发展等。在促销方面,推动数字化营销创新;重视品牌营销;迎合共享经济潮流等。这些优化建议对于促进宏光MINI EV新能源汽车更好开拓市场、维持市场份额、创造更优业绩有积极作用。Wuling Hongguang MINI EV is a new energy vehicle launched by SAIC-GM-Wuling Automobile Co., Ltd. in full consideration of market demand. It ranked first in China’s new energy market for 19 consecutive months in the initial stage of listing. However, with the changes in government policies and market, its competitiveness also declined. This paper comprehensively uses case analysis and literature research methods to study the marketing strategy of new energy vehicle Hongguang MINI EV. First of all, by using STP and 4P theory, the marketing status of Hongguang MINI EV was analyzed, and it was found that there were some problems in marketing, such as insufficient core competitiveness of products, difficulty in maintaining low-price marketing strategy with low profits, blind and inefficient promotional activities, too limited market positioning and relatively lagging digital marketing. Then, it provides optimization suggestions for the marketing of Hongguang MINI EV, including product improvement and product competitiveness. Promote technological upgrading, etc. In terms of price, differentiated pricing is adopted. In terms of channels, promote the development of all channels. In terms of promotion, promote digital marketing innovation;Attach importance to brand marketing;Cater to the trend of sharing economy. These optimization suggestions have a positive role in promoting Hongguang MINI EV new energy vehicles to better explore the market, maintain market share and create better performance.展开更多
文摘目的:建立荧光标记D1S1676、D2S441、D3S4529、D22S1045、amelogenin 5个mini短串联重复序列(Short tandem repeat,STR)基因座复合扩增体系,并对重庆地区汉族200名无关个体此5个基因座的遗传多态性进行研究分析。方法:分别用5’TAMRA标记D1S1676引物,5’6-FAM标记D2S441和D3S4529引物,5’HEX标记D22S1045引物,5’TET标记amelogenin引物,构建及优化符合扩增体系,运用3130基因分析仪检测并收集电泳结果数据,通过Gene Mapper v3.2.1软件计算扩增产物片段相对大小以及进行样本基因型分型。结果:5个miniSTR基因座荧光标记复合扩增体系每个位点都获得清晰的分型结果。对重庆地区汉族无关个体200份血样进行分型,5个基因座分别检出9、9、8、9、2个等位基因和103种基因型,其基因型分布均符Hardy-Weinberg平衡。其中D1S1676、D2S441、D3S4529、D22S1045基因座在重庆汉族群体的杂合度(Heterozygosity,Ho)分别依次为0.710、0.765、0.730、0.710;多态信息含量(Polymorphism information content,PIC)分别依次为:0.660 041、0.798 329、0.753 177、0.797 888;累计非父排除率(Exclusion probability of paternity,EP)为0.924 800 4,累计个人识别率为0.999 955 1。结论:荧光标记D1S1676、D2S441、D3S4529、D22S1045、amelogenin 5个miniSTR基因座复合扩增体系,每个基因座可获得准确分型;重庆汉族群体该5个基因座的遗传学数据,可作为群体遗传学和法医学研究与应用的基础资料。
文摘A Malian governors’ delegation led by Gen. Kafougouna Kone, minister of territorial administration and local communities, visited Beijing, Yunnan and Guangdong in early March at the invitation of the CPAFFC.
文摘五菱宏光MINI EV是上汽通用五菱汽车股份有限公司在充分考虑市场需求的情况下推出了一款新能源汽车,它上市初期连续19个月在中国新能源市场销量排名第一,然而,随着政府政策以及市场的变化,其竞争力也有所下降。本文综合运用案例分析和文献研究法等方法,对新能源汽车宏光MINI EV的营销策略进行了研究。首先,借助STP和4P理论研究分析了宏光MINI EV的营销现状,发现了其在营销方面存在产品核心竞争力不足,低利润难以维持低价营销策略,促销活动较盲目和低效,对市场定位过于局限以及数字营销相对滞后等问题。然后,为宏光MINI EV的营销提供了优化建议,包括在产品方面,改良产品,提升产品竞争力;推动技术升级等。在价格方面,采取差异化定价等。在渠道方面,推动全渠道发展等。在促销方面,推动数字化营销创新;重视品牌营销;迎合共享经济潮流等。这些优化建议对于促进宏光MINI EV新能源汽车更好开拓市场、维持市场份额、创造更优业绩有积极作用。Wuling Hongguang MINI EV is a new energy vehicle launched by SAIC-GM-Wuling Automobile Co., Ltd. in full consideration of market demand. It ranked first in China’s new energy market for 19 consecutive months in the initial stage of listing. However, with the changes in government policies and market, its competitiveness also declined. This paper comprehensively uses case analysis and literature research methods to study the marketing strategy of new energy vehicle Hongguang MINI EV. First of all, by using STP and 4P theory, the marketing status of Hongguang MINI EV was analyzed, and it was found that there were some problems in marketing, such as insufficient core competitiveness of products, difficulty in maintaining low-price marketing strategy with low profits, blind and inefficient promotional activities, too limited market positioning and relatively lagging digital marketing. Then, it provides optimization suggestions for the marketing of Hongguang MINI EV, including product improvement and product competitiveness. Promote technological upgrading, etc. In terms of price, differentiated pricing is adopted. In terms of channels, promote the development of all channels. In terms of promotion, promote digital marketing innovation;Attach importance to brand marketing;Cater to the trend of sharing economy. These optimization suggestions have a positive role in promoting Hongguang MINI EV new energy vehicles to better explore the market, maintain market share and create better performance.
