Remote sensing image super-resolution technology is pivotal for enhancing image quality in critical applications including environmental monitoring,urban planning,and disaster assessment.However,traditional methods ex...Remote sensing image super-resolution technology is pivotal for enhancing image quality in critical applications including environmental monitoring,urban planning,and disaster assessment.However,traditional methods exhibit deficiencies in detail recovery and noise suppression,particularly when processing complex landscapes(e.g.,forests,farmlands),leading to artifacts and spectral distortions that limit practical utility.To address this,we propose an enhanced Super-Resolution Generative Adversarial Network(SRGAN)framework featuring three key innovations:(1)Replacement of L1/L2 loss with a robust Charbonnier loss to suppress noise while preserving edge details via adaptive gradient balancing;(2)A multi-loss joint optimization strategy dynamically weighting Charbonnier loss(β=0.5),Visual Geometry Group(VGG)perceptual loss(α=1),and adversarial loss(γ=0.1)to synergize pixel-level accuracy and perceptual quality;(3)A multi-scale residual network(MSRN)capturing cross-scale texture features(e.g.,forest canopies,mountain contours).Validated on Sentinel-2(10 m)and SPOT-6/7(2.5 m)datasets covering 904 km2 in Motuo County,Xizang,our method outperforms the SRGAN baseline(SR4RS)with Peak Signal-to-Noise Ratio(PSNR)gains of 0.29 dB and Structural Similarity Index(SSIM)improvements of 3.08%on forest imagery.Visual comparisons confirm enhanced texture continuity despite marginal Learned Perceptual Image Patch Similarity(LPIPS)increases.The method significantly improves noise robustness and edge retention in complex geomorphology,demonstrating 18%faster response in forest fire early warning and providing high-resolution support for agricultural/urban monitoring.Future work will integrate spectral constraints and lightweight architectures.展开更多
The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life s...The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.展开更多
Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provi...Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provide valuable insights into the health status of an individual,accurate diagnosis,and efficient treatment strategies for diseases.Super-resolution microscopy(SRM)has recently emerged as a cutting-edge tool for the study of blood cells,providing numerous advantages over traditional methods for examining subcellular structures and proteins.In this paper,we focus on outlining the fundamental principles of various SRM techniques and their applications in both normal and diseased states of blood cells.Furthermore,future prospects of SRM techniques in the analysis of blood cells are also discussed.展开更多
BACKGROUND Deep learning-based super-resolution(SR)reconstruction can obtain high-quality images with more detailed information.AIM To compare multiparametric normal-resolution(NR)and SR magnetic resonance imaging(MRI...BACKGROUND Deep learning-based super-resolution(SR)reconstruction can obtain high-quality images with more detailed information.AIM To compare multiparametric normal-resolution(NR)and SR magnetic resonance imaging(MRI)in predicting the histopathologic grade in hepatocellular carcinoma.METHODS We retrospectively analyzed a total of 826 patients from two medical centers(training 459;validation 196;test 171).T2-weighted imaging,diffusion-weighted imaging,and portal venous phases were collected.Tumor segmentations were conducted automatically by 3D U-Net.Based on generative adversarial network,we utilized 3D SR reconstruction to produce SR MRI.Radiomics models were developed and validated by XGBoost and Catboost.The predictive efficiency was demonstrated by calibration curves,decision curve analysis,area under the curve(AUC)and net reclassification index(NRI).RESULTS We extracted 3045 radiomic features from both NR and SR MRI,retaining 29 and 28 features,respectively.For XGBoost models,SR MRI yielded higher AUC value than NR MRI in the validation and test cohorts(0.83 vs 0.79;0.80 vs 0.78),respectively.Consistent trends were seen in CatBoost models:SR MRI achieved AUCs of 0.89 and 0.80 compared to NR MRI’s 0.81 and 0.76.NRI indicated that the SR MRI models could improve the prediction accuracy by-1.6%to 20.9%compared to the NR MRI models.CONCLUSION Deep learning-based SR MRI could improve the predictive performance of histopathologic grade in HCC.It may be a powerful tool for better stratification management for patients with operable HCC.展开更多
Monitoring the dynamics of cellular pseudopodia at nanoscale has become essential for understanding their diverse and complex functions in living cells.This is made possible by combining single-molecule localization m...Monitoring the dynamics of cellular pseudopodia at nanoscale has become essential for understanding their diverse and complex functions in living cells.This is made possible by combining single-molecule localization microscopy(SMLM)with self-blinking dyes.However,existing self-blinking dyes often face limitations,such as nonspecific blinking and low photostability,which can bring background noise and yield erroneous localization signals,hindering their effectiveness for nanoscale visualization.Here,we present a method for long-term SMLM imaging of cellular pseudopodia dynamics using a blinkogenic probe that exhibits self-blinking activation upon molecular recognition.This approach enabled the precise tracking of various pseudopodia structures,including filopodia,lamellipodia,and(tunneling nanotubes)-nanoscale(TNTs),in living cells.We monitored the growth and fusion of filopodia,as well as the extension and shrinkage of lamellipodia,in real-time.Additionally,we identified two distinct fusion modes between filopodia and lamellipodia and captured the formation of TNTs and their interactions with filopodia,demonstrating the probe's utility in visualizing real-time pseudopodia dynamics at nanoscale.展开更多
Neutron capture event imaging is a novel technique that has the potential to substantially enhance the resolution of existing imaging systems.This study provides a measurement method for neutron capture event distribu...Neutron capture event imaging is a novel technique that has the potential to substantially enhance the resolution of existing imaging systems.This study provides a measurement method for neutron capture event distribution along with multiple reconstruction methods for super-resolution imaging.The proposed technology reduces the point-spread function of an imag-ing system through single-neutron detection and event reconstruction,thereby significantly improving imaging resolution.A single-neutron detection experiment was conducted using a highly practical and efficient^(6)LiF-ZnS scintillation screen of a cold neutron imaging device in the research reactor.In milliseconds of exposure time,a large number of weak light clusters and their distribution in the scintillation screen were recorded frame by frame,to complete single-neutron detection.Several reconstruction algorithms were proposed for the calculations.The location of neutron capture was calculated using several processing methods such as noise removal,filtering,spot segmentation,contour analysis,and local positioning.The proposed algorithm achieved a higher imaging resolution and faster reconstruction speed,and single-neutron super-resolution imaging was realized by combining single-neutron detection experiments and reconstruction calculations.The results show that the resolution of the 100μm thick^(6)LiF-ZnS scintillation screen can be improved from 125 to 40 microns.This indicates that the proposed single-neutron detection and calculation method is effective and can significantly improve imaging resolution.展开更多
Ultrasonic Lamb waves undergo complex mode conversion and diffraction at non-penetrating defects, such as plate corrosion and cracks. Lamb wave imaging has a resolution limit due to the guided wave dispersion characte...Ultrasonic Lamb waves undergo complex mode conversion and diffraction at non-penetrating defects, such as plate corrosion and cracks. Lamb wave imaging has a resolution limit due to the guided wave dispersion characteristics and Rayleigh criterion limitations. In this paper, a full convolutional network is designed to segment and reconstruct the received signals, enabling the automatic identification of target modalities. This approach eliminates clutter and mode conversion interference when calculating direct and accompanying acoustic fields in time-domain topological energy(TDTE) imaging.Subsequently, the measured accompanying acoustic field is reversed for adaptive focusing on defects and enhance the imaging quality. To circumvent the limitations of the Rayleigh criterion, the direct acoustic field and the accompanying acoustic field were fused to characterize the pixel distribution in the imaging region, achieving Lamb wave super-resolution imaging. Experimental results indicate that compared to the sign coherence factor-total focusing method(SCF-TFM),the proposed method achieves a 31.41% improvement in lateral resolution and a 29.53% increase in signal-to-noise ratio for single-blind-hole defects. In the case of multiple-blind-hole defects with spacings greater than the Rayleigh criterion resolution limit, it exhibits a 27.23% enhancement in signal-to-noise ratio. On the contrary, when the defect spacings are relatively smaller than the limit, this method has a higher resolution limit than SCF-TFM in super-resolution imaging.展开更多
Image super-resolution reconstruction technology is currently widely used in medical imaging,video surveillance,and industrial quality inspection.It not only enhances image quality but also improves details and visual...Image super-resolution reconstruction technology is currently widely used in medical imaging,video surveillance,and industrial quality inspection.It not only enhances image quality but also improves details and visual perception,significantly increasing the utility of low-resolution images.In this study,an improved image superresolution reconstruction model based on Generative Adversarial Networks(SRGAN)was proposed.This model introduced a channel and spatial attention mechanism(CSAB)in the generator,allowing it to effectively leverage the information from the input image to enhance feature representations and capture important details.The discriminator was designed with an improved PatchGAN architecture,which more accurately captured local details and texture information of the image.With these enhanced generator and discriminator architectures and an optimized loss function design,this method demonstrated superior performance in image quality assessment metrics.Experimental results showed that this model outperforms traditional methods,presenting more detailed and realistic image details in the visual effects.展开更多
Significant advancements have been achieved in the field of Single Image Super-Resolution(SISR)through the utilization of Convolutional Neural Networks(CNNs)to attain state-of-the-art performance.Recent efforts have e...Significant advancements have been achieved in the field of Single Image Super-Resolution(SISR)through the utilization of Convolutional Neural Networks(CNNs)to attain state-of-the-art performance.Recent efforts have explored the incorporation of Transformers to augment network performance in SISR.However,the high computational cost of Transformers makes them less suitable for deployment on lightweight devices.Moreover,the majority of enhancements for CNNs rely predominantly on small spatial convolutions,thereby neglecting the potential advantages of large kernel convolution.In this paper,the authors propose a Multi-Perception Large Kernel convNet(MPLKN)which delves into the exploration of large kernel convolution.Specifically,the authors have architected a Multi-Perception Large Kernel(MPLK)module aimed at extracting multi-scale features and employ a stepwise feature fusion strategy to seamlessly integrate these features.In addition,to enhance the network's capacity for nonlinear spatial information processing,the authors have designed a Spatial-Channel Gated Feed-forward Network(SCGFN)that is capable of adapting to feature interactions across both spatial and channel dimensions.Experimental results demonstrate that MPLKN outperforms other lightweight image super-resolution models while maintaining a minimal number of parameters and FLOPs.展开更多
Infrared imaging technology has been widely adopted in various fields,such as military reconnaissance,medical diagnosis,and security monitoring,due to its excellent ability to penetrate smoke and fog.However,the preva...Infrared imaging technology has been widely adopted in various fields,such as military reconnaissance,medical diagnosis,and security monitoring,due to its excellent ability to penetrate smoke and fog.However,the prevalent low resolution of infrared images severely limits the accurate interpretation of their contents.In addition,deploying super-resolution models on resource-constrained devices faces significant challenges.To address these issues,this study proposes a lightweight super-resolution network for infrared images based on an adaptive attention mechanism.The network’s dynamic weighting module automatically adjusts the weights of the attention and nonattention branch outputs based on the network’s characteristics at different levels.Among them,the attention branch is further subdivided into pixel attention and brightness-texture attention,which are specialized for extracting the most informative features in infrared images.Meanwhile,the non-attention branch supplements the extraction of those neglected features to enhance the comprehensiveness of the features.Through ablation experiments,we verify the effectiveness of the proposed module.Finally,through experiments on two datasets,FLIR and Thermal101,qualitative and quantitative results demonstrate that the model can effectively recover high-frequency details of infrared images and significantly improve image resolution.In detail,compared with the suboptimal method,we have reduced the number of parameters by 30%and improved the model performance.When the scale factor is 2,the peak signal-tonoise ratio of the test datasets FLIR and Thermal101 is improved by 0.09 and 0.15 dB,respectively.When the scale factor is 4,it is improved by 0.05 and 0.09 dB,respectively.In addition,due to the lightweight design of the network structure,it has a low computational cost.It is suitable for deployment on edge devices,thus effectively enhancing the sensing performance of infrared imaging devices.展开更多
The application of image super-resolution(SR)has brought significant assistance in the medical field,aiding doctors to make more precise diagnoses.However,solely relying on a convolutional neural network(CNN)for image...The application of image super-resolution(SR)has brought significant assistance in the medical field,aiding doctors to make more precise diagnoses.However,solely relying on a convolutional neural network(CNN)for image SR may lead to issues such as blurry details and excessive smoothness.To address the limitations,we proposed an algorithm based on the generative adversarial network(GAN)framework.In the generator network,three different sizes of convolutions connected by a residual dense structure were used to extract detailed features,and an attention mechanism combined with dual channel and spatial information was applied to concentrate the computing power on crucial areas.In the discriminator network,using InstanceNorm to normalize tensors sped up the training process while retaining feature information.The experimental results demonstrate that our algorithm achieves higher peak signal-to-noise ratio(PSNR)and structural similarity index measure(SSIM)compared to other methods,resulting in an improved visual quality.展开更多
Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the dis...Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by He La cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution(lysosomal or mitochondrial) in a time-dependent manner. Moreover,lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species(ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.展开更多
Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resol...Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential.Here,we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives.Using structured illumination microscopy(SIM),we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution.Furthermore,the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale.Convincingly,the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.展开更多
The need for temporal resolution and long-term stability in super-resolution fluorescence imaging has motivated research to improve the photostability of fluorescent probes.Due to the inevitable photobleaching of fluo...The need for temporal resolution and long-term stability in super-resolution fluorescence imaging has motivated research to improve the photostability of fluorescent probes.Due to the inevitable photobleaching of fluorophores,it is difficult to obtain long-term super-resolution imaging regardless of the self-healing strategy of introducing peroxide scavengers or the strategy of fluorophore structure modification to suppress TICT formation.The buffered fluorogenic probe uses the intact probes in the buffer pool to continuously replace the photobleached ones in the target,which greatly improves the photostability and enables stable dynamic super-resolution imaging for a long time.But the buffering capacity comes at the expense of reducing the number of fluorescent probes in targets,resulting in low staining fluorescence intensity.In this paper,we selected BODIPY 493,a lipid droplet probe with high fluorescence brightness,to explore the dynamic process of lipid droplet staining of this probe in cells.We found that BODIPY 493 only needs very low laser power for lipid droplet imaging due to the high molecular accumulation in lipid droplets and the high brightness,and the spatiotemporal resolution is greatly improved.More importantly,we found that BODIPY 493 also has a certain buffering capacity,which enables BODIPY 493 to be used for super-resolution imaging of lipid droplet dynamics.This work reminds researchers to coordinate the buffering capacity and brightness of fluorogenic probes.展开更多
Purpose: To apply and evaluate a super-resolution scheme based on the super-resolution convolutional neural network (SRCNN) for enhancing image resolution in digital mammograms. Materials and Methods: A total of 711 m...Purpose: To apply and evaluate a super-resolution scheme based on the super-resolution convolutional neural network (SRCNN) for enhancing image resolution in digital mammograms. Materials and Methods: A total of 711 mediolateral oblique (MLO) images including breast lesions were sampled from the Curated Breast Imaging Subset of the Digital Database for Screening Mammography (CBIS-DDSM). We first trained the super-resolution convolutional neural network (SRCNN), which is a deep-learning based super-resolution method. Using this trained SRCNN, high-resolution images were reconstructed from low-resolution images. We compared the image quality of the super-resolution method and that obtained using the linear interpolation methods (nearest neighbor and bilinear interpolations). To investigate the relationship between the image quality of the SRCNN-processed images and the clinical features of the mammographic lesions, we compared the image quality yielded by implementing the SRCNN, in terms of the breast density, the Breast Imaging-Reporting and Data System (BI-RADS) assessment, and the verified pathology information. For quantitative evaluation, peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) were measured to assess the image restoration quality and the perceived image quality. Results: The super-resolution image quality yielded by the SRCNN was significantly higher than that obtained using linear interpolation methods (p p Conclusion: SRCNN can significantly outperform conventional interpolation methods for enhancing image resolution in digital mammography. SRCNN can significantly improve the image quality of magnified mammograms, especially in dense breasts, high-risk BI-RADS assessment groups, and pathology-verified malignant cases.展开更多
A full-polarimetric super-resolution algorithm with spatial smoothing processing is presented for one-dimensional（1-D）radar imaging.The coherence between scattering centers is minimized by using spatial smoothing pr...A full-polarimetric super-resolution algorithm with spatial smoothing processing is presented for one-dimensional（1-D）radar imaging.The coherence between scattering centers is minimized by using spatial smoothing processing（SSP）.Then the range and polarimetric scattering matrix of the scattering centers are estimated.The impact of different lengths of the smoothing window on the imaging quality is mainly analyzed with different signal-to-noise ratios（SNR）.Simulation and experimental results show that an improved radar super-resolution range profile and more precise estimation can be obtained by adjusting the length of the smoothing window under different SNR conditions.展开更多
Super-resolution imaging is vital for optical applications, such as high capacity information transmission, real-time bio-molecular imaging, and nanolithography. In recent years, technologies and methods of super-reso...Super-resolution imaging is vital for optical applications, such as high capacity information transmission, real-time bio-molecular imaging, and nanolithography. In recent years, technologies and methods of super-resolution imaging have attracted much attention. Different kinds of novel lenses, from the superlens to the super-oscillatory lens, have been designed and fabricated to break through the diffraction limit. However, the effect of the super-resolution imaging in these lenses is not satisfactory due to intrinsic loss, aberration, large sidebands, and so on. Moreover, these lenses also cannot realize multiple super-resolution imaging. In this research, we introduce the solid immersion mechanism to Mikaelian lens(ML) for multiple super-resolution imaging. The effect is robust and valid for broadband frequencies. Based on conformal transformation optics as a bridge linking the solid immersion ML and generalized Maxwell's fish-eye lens(GMFEL), we also discovered the effect of multiple super-resolution imaging in the solid immersion GMFEL.展开更多
Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes,including cell adhesion,recognition and signaling,viral and bacterial infection,in°ammation and metastasis.However,owin...Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes,including cell adhesion,recognition and signaling,viral and bacterial infection,in°ammation and metastasis.However,owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis,glycoscience is the least understood¯eld compared with genomics and proteomics.Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods,the distribution and role of carbohydrates in cell membranes remain elusive.This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging,the labeling strategies of carbohydrates,and the recent applications of super-resolution imaging in glycoscience,which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.展开更多
Single image super-resolution has attracted increasing attention and has a wide range of applications in satellite imaging, medical imaging, computer vision, security surveillance imaging, remote sensing, objection de...Single image super-resolution has attracted increasing attention and has a wide range of applications in satellite imaging, medical imaging, computer vision, security surveillance imaging, remote sensing, objection detection, and recognition. Recently, deep learning techniques have emerged and blossomed, producing " the state-of-the-art” in many domains. Due to their capability in feature extraction and mapping, it is very helpful to predict high-frequency details lost in low-resolution images. In this paper, we give an overview of recent advances in deep learning-based models and methods that have been applied to single image super-resolution tasks. We also summarize, compare and discuss various models from the past and present for comprehensive understanding and finally provide open problems and possible directions for future research.展开更多
Hyperspectral image super-resolution,which refers to reconstructing the high-resolution hyperspectral image from the input low-resolution observation,aims to improve the spatial resolution of the hyperspectral image,w...Hyperspectral image super-resolution,which refers to reconstructing the high-resolution hyperspectral image from the input low-resolution observation,aims to improve the spatial resolution of the hyperspectral image,which is beneficial for subsequent applications.The development of deep learning has promoted significant progress in hyperspectral image super-resolution,and the powerful expression capabilities of deep neural networks make the predicted results more reliable.Recently,several latest deep learning technologies have made the hyperspectral image super-resolution method explode.However,a comprehensive review and analysis of the latest deep learning methods from the hyperspectral image super-resolution perspective is absent.To this end,in this survey,we first introduce the concept of hyperspectral image super-resolution and classify the methods from the perspectives with or without auxiliary information.Then,we review the learning-based methods in three categories,including single hyperspectral image super-resolution,panchromatic-based hyperspectral image super-resolution,and multispectral-based hyperspectral image super-resolution.Subsequently,we summarize the commonly used hyperspectral dataset,and the evaluations for some representative methods in three categories are performed qualitatively and quantitatively.Moreover,we briefly introduce several typical applications of hyperspectral image super-resolution,including ground object classification,urban change detection,and ecosystem monitoring.Finally,we provide the conclusion and challenges in existing learning-based methods,looking forward to potential future research directions.展开更多
基金This study was supported by:Inner Mongolia Academy of Forestry Sciences Open Research Project(Grant No.KF2024MS03)The Project to Improve the Scientific Research Capacity of the Inner Mongolia Academy of Forestry Sciences(Grant No.2024NLTS04)The Innovation and Entrepreneurship Training Program for Undergraduates of Beijing Forestry University(Grant No.X202410022268).
文摘Remote sensing image super-resolution technology is pivotal for enhancing image quality in critical applications including environmental monitoring,urban planning,and disaster assessment.However,traditional methods exhibit deficiencies in detail recovery and noise suppression,particularly when processing complex landscapes(e.g.,forests,farmlands),leading to artifacts and spectral distortions that limit practical utility.To address this,we propose an enhanced Super-Resolution Generative Adversarial Network(SRGAN)framework featuring three key innovations:(1)Replacement of L1/L2 loss with a robust Charbonnier loss to suppress noise while preserving edge details via adaptive gradient balancing;(2)A multi-loss joint optimization strategy dynamically weighting Charbonnier loss(β=0.5),Visual Geometry Group(VGG)perceptual loss(α=1),and adversarial loss(γ=0.1)to synergize pixel-level accuracy and perceptual quality;(3)A multi-scale residual network(MSRN)capturing cross-scale texture features(e.g.,forest canopies,mountain contours).Validated on Sentinel-2(10 m)and SPOT-6/7(2.5 m)datasets covering 904 km2 in Motuo County,Xizang,our method outperforms the SRGAN baseline(SR4RS)with Peak Signal-to-Noise Ratio(PSNR)gains of 0.29 dB and Structural Similarity Index(SSIM)improvements of 3.08%on forest imagery.Visual comparisons confirm enhanced texture continuity despite marginal Learned Perceptual Image Patch Similarity(LPIPS)increases.The method significantly improves noise robustness and edge retention in complex geomorphology,demonstrating 18%faster response in forest fire early warning and providing high-resolution support for agricultural/urban monitoring.Future work will integrate spectral constraints and lightweight architectures.
基金supported by the following grants:National Natural Science Foundation of China(grant nos.92354305,32271428,and 32201132)National Key R&D Program of China(grant no.2022YFC3401100)+1 种基金Fund for Knowledge Innovation of Wuhan Science and Technology Bureau(grant no.2022020801010558)Director Fund of WNLO.
文摘The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.
基金supported by the following grants:National Key R&D Program of China(Grant no.2022YFC3401100)National Natural Science Foundation of China(Grant nos.32271428,92054110,32201132 and 31600692).
文摘Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provide valuable insights into the health status of an individual,accurate diagnosis,and efficient treatment strategies for diseases.Super-resolution microscopy(SRM)has recently emerged as a cutting-edge tool for the study of blood cells,providing numerous advantages over traditional methods for examining subcellular structures and proteins.In this paper,we focus on outlining the fundamental principles of various SRM techniques and their applications in both normal and diseased states of blood cells.Furthermore,future prospects of SRM techniques in the analysis of blood cells are also discussed.
基金Supported by AI+Health Collaborative Innovation Cultivation Project of Beijing City,No.Z221100003522005.
文摘BACKGROUND Deep learning-based super-resolution(SR)reconstruction can obtain high-quality images with more detailed information.AIM To compare multiparametric normal-resolution(NR)and SR magnetic resonance imaging(MRI)in predicting the histopathologic grade in hepatocellular carcinoma.METHODS We retrospectively analyzed a total of 826 patients from two medical centers(training 459;validation 196;test 171).T2-weighted imaging,diffusion-weighted imaging,and portal venous phases were collected.Tumor segmentations were conducted automatically by 3D U-Net.Based on generative adversarial network,we utilized 3D SR reconstruction to produce SR MRI.Radiomics models were developed and validated by XGBoost and Catboost.The predictive efficiency was demonstrated by calibration curves,decision curve analysis,area under the curve(AUC)and net reclassification index(NRI).RESULTS We extracted 3045 radiomic features from both NR and SR MRI,retaining 29 and 28 features,respectively.For XGBoost models,SR MRI yielded higher AUC value than NR MRI in the validation and test cohorts(0.83 vs 0.79;0.80 vs 0.78),respectively.Consistent trends were seen in CatBoost models:SR MRI achieved AUCs of 0.89 and 0.80 compared to NR MRI’s 0.81 and 0.76.NRI indicated that the SR MRI models could improve the prediction accuracy by-1.6%to 20.9%compared to the NR MRI models.CONCLUSION Deep learning-based SR MRI could improve the predictive performance of histopathologic grade in HCC.It may be a powerful tool for better stratification management for patients with operable HCC.
基金supported by the National Natural Science Foundation of China(Nos.22225806,22078314,22278394,22378385)Dalian Institute of Chemical Physics(Nos.DICPI202227,DICPI202436)。
文摘Monitoring the dynamics of cellular pseudopodia at nanoscale has become essential for understanding their diverse and complex functions in living cells.This is made possible by combining single-molecule localization microscopy(SMLM)with self-blinking dyes.However,existing self-blinking dyes often face limitations,such as nonspecific blinking and low photostability,which can bring background noise and yield erroneous localization signals,hindering their effectiveness for nanoscale visualization.Here,we present a method for long-term SMLM imaging of cellular pseudopodia dynamics using a blinkogenic probe that exhibits self-blinking activation upon molecular recognition.This approach enabled the precise tracking of various pseudopodia structures,including filopodia,lamellipodia,and(tunneling nanotubes)-nanoscale(TNTs),in living cells.We monitored the growth and fusion of filopodia,as well as the extension and shrinkage of lamellipodia,in real-time.Additionally,we identified two distinct fusion modes between filopodia and lamellipodia and captured the formation of TNTs and their interactions with filopodia,demonstrating the probe's utility in visualizing real-time pseudopodia dynamics at nanoscale.
基金supported by the National Natural Science Foundation of China(Nos.12205271,12075217,U20B2011,and 51978218)Sichuan Science and Technology Program(No.2019ZDZX0010)the National Key R&D Program of China(No.2022YFA1604002).
文摘Neutron capture event imaging is a novel technique that has the potential to substantially enhance the resolution of existing imaging systems.This study provides a measurement method for neutron capture event distribution along with multiple reconstruction methods for super-resolution imaging.The proposed technology reduces the point-spread function of an imag-ing system through single-neutron detection and event reconstruction,thereby significantly improving imaging resolution.A single-neutron detection experiment was conducted using a highly practical and efficient^(6)LiF-ZnS scintillation screen of a cold neutron imaging device in the research reactor.In milliseconds of exposure time,a large number of weak light clusters and their distribution in the scintillation screen were recorded frame by frame,to complete single-neutron detection.Several reconstruction algorithms were proposed for the calculations.The location of neutron capture was calculated using several processing methods such as noise removal,filtering,spot segmentation,contour analysis,and local positioning.The proposed algorithm achieved a higher imaging resolution and faster reconstruction speed,and single-neutron super-resolution imaging was realized by combining single-neutron detection experiments and reconstruction calculations.The results show that the resolution of the 100μm thick^(6)LiF-ZnS scintillation screen can be improved from 125 to 40 microns.This indicates that the proposed single-neutron detection and calculation method is effective and can significantly improve imaging resolution.
基金Project supported by the National Natural Science Foundation of China (Grant No. 12174085)the Key Research and Development Project of Changzhou, Jiangsu Province, China (Grant No. CE20235054)the Postgraduate Research and Practice Innovation Program of Jiangsu Province, China (Grant No. KYCX24 0833)。
文摘Ultrasonic Lamb waves undergo complex mode conversion and diffraction at non-penetrating defects, such as plate corrosion and cracks. Lamb wave imaging has a resolution limit due to the guided wave dispersion characteristics and Rayleigh criterion limitations. In this paper, a full convolutional network is designed to segment and reconstruct the received signals, enabling the automatic identification of target modalities. This approach eliminates clutter and mode conversion interference when calculating direct and accompanying acoustic fields in time-domain topological energy(TDTE) imaging.Subsequently, the measured accompanying acoustic field is reversed for adaptive focusing on defects and enhance the imaging quality. To circumvent the limitations of the Rayleigh criterion, the direct acoustic field and the accompanying acoustic field were fused to characterize the pixel distribution in the imaging region, achieving Lamb wave super-resolution imaging. Experimental results indicate that compared to the sign coherence factor-total focusing method(SCF-TFM),the proposed method achieves a 31.41% improvement in lateral resolution and a 29.53% increase in signal-to-noise ratio for single-blind-hole defects. In the case of multiple-blind-hole defects with spacings greater than the Rayleigh criterion resolution limit, it exhibits a 27.23% enhancement in signal-to-noise ratio. On the contrary, when the defect spacings are relatively smaller than the limit, this method has a higher resolution limit than SCF-TFM in super-resolution imaging.
文摘Image super-resolution reconstruction technology is currently widely used in medical imaging,video surveillance,and industrial quality inspection.It not only enhances image quality but also improves details and visual perception,significantly increasing the utility of low-resolution images.In this study,an improved image superresolution reconstruction model based on Generative Adversarial Networks(SRGAN)was proposed.This model introduced a channel and spatial attention mechanism(CSAB)in the generator,allowing it to effectively leverage the information from the input image to enhance feature representations and capture important details.The discriminator was designed with an improved PatchGAN architecture,which more accurately captured local details and texture information of the image.With these enhanced generator and discriminator architectures and an optimized loss function design,this method demonstrated superior performance in image quality assessment metrics.Experimental results showed that this model outperforms traditional methods,presenting more detailed and realistic image details in the visual effects.
文摘Significant advancements have been achieved in the field of Single Image Super-Resolution(SISR)through the utilization of Convolutional Neural Networks(CNNs)to attain state-of-the-art performance.Recent efforts have explored the incorporation of Transformers to augment network performance in SISR.However,the high computational cost of Transformers makes them less suitable for deployment on lightweight devices.Moreover,the majority of enhancements for CNNs rely predominantly on small spatial convolutions,thereby neglecting the potential advantages of large kernel convolution.In this paper,the authors propose a Multi-Perception Large Kernel convNet(MPLKN)which delves into the exploration of large kernel convolution.Specifically,the authors have architected a Multi-Perception Large Kernel(MPLK)module aimed at extracting multi-scale features and employ a stepwise feature fusion strategy to seamlessly integrate these features.In addition,to enhance the network's capacity for nonlinear spatial information processing,the authors have designed a Spatial-Channel Gated Feed-forward Network(SCGFN)that is capable of adapting to feature interactions across both spatial and channel dimensions.Experimental results demonstrate that MPLKN outperforms other lightweight image super-resolution models while maintaining a minimal number of parameters and FLOPs.
基金funded in part by theHenan ProvinceKeyR&DProgramProject,“Research and Application Demonstration of Class Ⅱ Superlattice Medium Wave High Temperature Infrared Detector Technology”under Grant No.231111210400.
文摘Infrared imaging technology has been widely adopted in various fields,such as military reconnaissance,medical diagnosis,and security monitoring,due to its excellent ability to penetrate smoke and fog.However,the prevalent low resolution of infrared images severely limits the accurate interpretation of their contents.In addition,deploying super-resolution models on resource-constrained devices faces significant challenges.To address these issues,this study proposes a lightweight super-resolution network for infrared images based on an adaptive attention mechanism.The network’s dynamic weighting module automatically adjusts the weights of the attention and nonattention branch outputs based on the network’s characteristics at different levels.Among them,the attention branch is further subdivided into pixel attention and brightness-texture attention,which are specialized for extracting the most informative features in infrared images.Meanwhile,the non-attention branch supplements the extraction of those neglected features to enhance the comprehensiveness of the features.Through ablation experiments,we verify the effectiveness of the proposed module.Finally,through experiments on two datasets,FLIR and Thermal101,qualitative and quantitative results demonstrate that the model can effectively recover high-frequency details of infrared images and significantly improve image resolution.In detail,compared with the suboptimal method,we have reduced the number of parameters by 30%and improved the model performance.When the scale factor is 2,the peak signal-tonoise ratio of the test datasets FLIR and Thermal101 is improved by 0.09 and 0.15 dB,respectively.When the scale factor is 4,it is improved by 0.05 and 0.09 dB,respectively.In addition,due to the lightweight design of the network structure,it has a low computational cost.It is suitable for deployment on edge devices,thus effectively enhancing the sensing performance of infrared imaging devices.
文摘The application of image super-resolution(SR)has brought significant assistance in the medical field,aiding doctors to make more precise diagnoses.However,solely relying on a convolutional neural network(CNN)for image SR may lead to issues such as blurry details and excessive smoothness.To address the limitations,we proposed an algorithm based on the generative adversarial network(GAN)framework.In the generator network,three different sizes of convolutions connected by a residual dense structure were used to extract detailed features,and an attention mechanism combined with dual channel and spatial information was applied to concentrate the computing power on crucial areas.In the discriminator network,using InstanceNorm to normalize tensors sped up the training process while retaining feature information.The experimental results demonstrate that our algorithm achieves higher peak signal-to-noise ratio(PSNR)and structural similarity index measure(SSIM)compared to other methods,resulting in an improved visual quality.
基金supported by National Natural Science Foundation of China (Nos. 22107059, 21801158, 81870283, 82070382)Program of Taishan Scholars Programme (No. 20190979)+3 种基金Academic Promotion Programme of Shandong First Medical University (No.2019LJ003)National Postdoctoral Program for Innovative Talents(No. BX2021123)The China Postdoctoral Science Foundation (No.2021M691505)the Jiangsu Postdoctoral Research Funding Program (No. 2021K125B)。
文摘Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by He La cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution(lysosomal or mitochondrial) in a time-dependent manner. Moreover,lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species(ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.
基金the National Natural Science Foundation of China(Nos.21878286,21576043,21878286)Dalian Institute of Chemical Physics,Chinese Academy of Sciences(Nos.I201938,ZZBS201805)。
文摘Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential.Here,we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives.Using structured illumination microscopy(SIM),we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution.Furthermore,the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale.Convincingly,the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.
基金supported by the National Natural Science Foundation of China(Nos.22078314,21878286,21908216).
文摘The need for temporal resolution and long-term stability in super-resolution fluorescence imaging has motivated research to improve the photostability of fluorescent probes.Due to the inevitable photobleaching of fluorophores,it is difficult to obtain long-term super-resolution imaging regardless of the self-healing strategy of introducing peroxide scavengers or the strategy of fluorophore structure modification to suppress TICT formation.The buffered fluorogenic probe uses the intact probes in the buffer pool to continuously replace the photobleached ones in the target,which greatly improves the photostability and enables stable dynamic super-resolution imaging for a long time.But the buffering capacity comes at the expense of reducing the number of fluorescent probes in targets,resulting in low staining fluorescence intensity.In this paper,we selected BODIPY 493,a lipid droplet probe with high fluorescence brightness,to explore the dynamic process of lipid droplet staining of this probe in cells.We found that BODIPY 493 only needs very low laser power for lipid droplet imaging due to the high molecular accumulation in lipid droplets and the high brightness,and the spatiotemporal resolution is greatly improved.More importantly,we found that BODIPY 493 also has a certain buffering capacity,which enables BODIPY 493 to be used for super-resolution imaging of lipid droplet dynamics.This work reminds researchers to coordinate the buffering capacity and brightness of fluorogenic probes.
文摘Purpose: To apply and evaluate a super-resolution scheme based on the super-resolution convolutional neural network (SRCNN) for enhancing image resolution in digital mammograms. Materials and Methods: A total of 711 mediolateral oblique (MLO) images including breast lesions were sampled from the Curated Breast Imaging Subset of the Digital Database for Screening Mammography (CBIS-DDSM). We first trained the super-resolution convolutional neural network (SRCNN), which is a deep-learning based super-resolution method. Using this trained SRCNN, high-resolution images were reconstructed from low-resolution images. We compared the image quality of the super-resolution method and that obtained using the linear interpolation methods (nearest neighbor and bilinear interpolations). To investigate the relationship between the image quality of the SRCNN-processed images and the clinical features of the mammographic lesions, we compared the image quality yielded by implementing the SRCNN, in terms of the breast density, the Breast Imaging-Reporting and Data System (BI-RADS) assessment, and the verified pathology information. For quantitative evaluation, peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) were measured to assess the image restoration quality and the perceived image quality. Results: The super-resolution image quality yielded by the SRCNN was significantly higher than that obtained using linear interpolation methods (p p Conclusion: SRCNN can significantly outperform conventional interpolation methods for enhancing image resolution in digital mammography. SRCNN can significantly improve the image quality of magnified mammograms, especially in dense breasts, high-risk BI-RADS assessment groups, and pathology-verified malignant cases.
基金Supported by the National Naturral Science Foundation of China(61301191)
文摘A full-polarimetric super-resolution algorithm with spatial smoothing processing is presented for one-dimensional（1-D）radar imaging.The coherence between scattering centers is minimized by using spatial smoothing processing（SSP）.Then the range and polarimetric scattering matrix of the scattering centers are estimated.The impact of different lengths of the smoothing window on the imaging quality is mainly analyzed with different signal-to-noise ratios（SNR）.Simulation and experimental results show that an improved radar super-resolution range profile and more precise estimation can be obtained by adjusting the length of the smoothing window under different SNR conditions.
基金Project supported by the National Natural Science Foundation of China (Grant No. 92050102)the National Key Research and Development Program of China (Grant No. 2020YFA0710100)the Fundamental Research Funds for Central Universities, China (Grant Nos. 20720200074, 20720220134, 202006310051, and 20720220033)。
文摘Super-resolution imaging is vital for optical applications, such as high capacity information transmission, real-time bio-molecular imaging, and nanolithography. In recent years, technologies and methods of super-resolution imaging have attracted much attention. Different kinds of novel lenses, from the superlens to the super-oscillatory lens, have been designed and fabricated to break through the diffraction limit. However, the effect of the super-resolution imaging in these lenses is not satisfactory due to intrinsic loss, aberration, large sidebands, and so on. Moreover, these lenses also cannot realize multiple super-resolution imaging. In this research, we introduce the solid immersion mechanism to Mikaelian lens(ML) for multiple super-resolution imaging. The effect is robust and valid for broadband frequencies. Based on conformal transformation optics as a bridge linking the solid immersion ML and generalized Maxwell's fish-eye lens(GMFEL), we also discovered the effect of multiple super-resolution imaging in the solid immersion GMFEL.
基金This work was supported by NSFC (Grants 31330082,21373200,21525314)the Instrument Developing project of the Chinese Academy of Sciences (Grant YZ201455).
文摘Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes,including cell adhesion,recognition and signaling,viral and bacterial infection,in°ammation and metastasis.However,owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis,glycoscience is the least understood¯eld compared with genomics and proteomics.Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods,the distribution and role of carbohydrates in cell membranes remain elusive.This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging,the labeling strategies of carbohydrates,and the recent applications of super-resolution imaging in glycoscience,which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.
基金the support from the Shanxi Hundred People Plan of China
文摘Single image super-resolution has attracted increasing attention and has a wide range of applications in satellite imaging, medical imaging, computer vision, security surveillance imaging, remote sensing, objection detection, and recognition. Recently, deep learning techniques have emerged and blossomed, producing " the state-of-the-art” in many domains. Due to their capability in feature extraction and mapping, it is very helpful to predict high-frequency details lost in low-resolution images. In this paper, we give an overview of recent advances in deep learning-based models and methods that have been applied to single image super-resolution tasks. We also summarize, compare and discuss various models from the past and present for comprehensive understanding and finally provide open problems and possible directions for future research.
基金supported in part by the National Natural Science Foundation of China(62276192)。
文摘Hyperspectral image super-resolution,which refers to reconstructing the high-resolution hyperspectral image from the input low-resolution observation,aims to improve the spatial resolution of the hyperspectral image,which is beneficial for subsequent applications.The development of deep learning has promoted significant progress in hyperspectral image super-resolution,and the powerful expression capabilities of deep neural networks make the predicted results more reliable.Recently,several latest deep learning technologies have made the hyperspectral image super-resolution method explode.However,a comprehensive review and analysis of the latest deep learning methods from the hyperspectral image super-resolution perspective is absent.To this end,in this survey,we first introduce the concept of hyperspectral image super-resolution and classify the methods from the perspectives with or without auxiliary information.Then,we review the learning-based methods in three categories,including single hyperspectral image super-resolution,panchromatic-based hyperspectral image super-resolution,and multispectral-based hyperspectral image super-resolution.Subsequently,we summarize the commonly used hyperspectral dataset,and the evaluations for some representative methods in three categories are performed qualitatively and quantitatively.Moreover,we briefly introduce several typical applications of hyperspectral image super-resolution,including ground object classification,urban change detection,and ecosystem monitoring.Finally,we provide the conclusion and challenges in existing learning-based methods,looking forward to potential future research directions.
