A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant...A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 μ column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ± 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.展开更多
A method is described to isolate mfinosine degrading bacteria from mmen juice from German steers which had never been ted on either leucaena or mimosine. The isolate was cultivated in continuous culture in a bench top...A method is described to isolate mfinosine degrading bacteria from mmen juice from German steers which had never been ted on either leucaena or mimosine. The isolate was cultivated in continuous culture in a bench top fermenter in Medium 98-5 amended with an increasing amount of mimosine for 16 days. Degradation of mimosine was determined with a colorrinetfic method Incubated at 39 ℃ under anaerobic conditions, the isolate showed complete degradation of mimosine within one ~veek. The bacteriological pure isolate is an aero-tolerant gram-negative cocco-bacillus. By DNA sequencing analysis it belongs to genus Klebsiella. It was multiplied hi brain-heart-infusion, entrapped in alginate, and dried （37 ℃）. Feeding trials in sheep in Myalmlar showed complete innocuity＇ and complete detoxification of mimosine from leucaena leaves.展开更多
Evidence suggests that increased level/aggregation of beta-amyloid(Aβ)peptides initiate neurodegeneration and subsequent development of Alzheimer’s disease(AD).At present,there is no effective treatment for AD.In th...Evidence suggests that increased level/aggregation of beta-amyloid(Aβ)peptides initiate neurodegeneration and subsequent development of Alzheimer’s disease(AD).At present,there is no effective treatment for AD.In this study,we reported the effects of gold nanoparticles surface-functionalized with a plant-based amino acid mimosine(Mimo-AuNPs),which is found to cross the blood-brain barrier,on the Aβfibrillization process and toxicity.Thioflavin T kinetic assays,fluorescence imaging and electron microscopy data showed that Mimo-AuNPs were able to suppress the spontaneous and seed-induced A_(1-42) aggregation.Spectroscopic studies,molecular docking and biochemical analyses further revealed that Mimo-AuNPs stabilize A_(1-42) to remain in its monomeric state by interacting with the hydrophobic domain of A_(1-42)(i.e.,Lys16 to Ala21)there by preventing a conformational shift towards theβ-sheet structure.Additionally,Mimo-AuNPs were found to trigger the disassembly of matured A_(1-42) fibers and increased neuronal viability by reducing phosphorylation of tau protein and the production of oxyradicals.Collectively,these results reveal that the surface-functionalization of gold nanoparticles with mimosine can attenuate Aβfibrillization and neuronal toxicity.Thus,we propose Mimo-AuNPs may be used as a potential treatment strategy towards AD-related pathologies.展开更多
Mimosine, a plant amino acid, is a reversible cell cycle inhibitor. Biochemical studies have indicated that mimosine may act at multiple levels near the Gl/S interface. By using mi-croinjection technique, it is shown ...Mimosine, a plant amino acid, is a reversible cell cycle inhibitor. Biochemical studies have indicated that mimosine may act at multiple levels near the Gl/S interface. By using mi-croinjection technique, it is shown that mimosine can also inhibit the replication of Xenopus ribo-some DNA (rDNA) plasmid reversibly. The high structure similarity between mimosine展开更多
Objective:To evaluate whether hypoxia inducible factor(HIF-1α) targeting pharmacological drugs,echinomycin,resveratrol and CdCl_2 which inhibit HIF-1α stimulation,and mimosine,which enhances the stability of HIF-1α...Objective:To evaluate whether hypoxia inducible factor(HIF-1α) targeting pharmacological drugs,echinomycin,resveratrol and CdCl_2 which inhibit HIF-1α stimulation,and mimosine,which enhances the stability of HIF-1α present antileishmanial properties.Methods:The leishmanicidal effect of drugs was evaluated in mouse macrophages and Balb/c mouse model for cutaneous leishmaniosis.Results:Resveratrol and CdCl_2 reduced the parasite load [IC50,(27.3±2.25) μM and(24.8±0.95) μM,respectively].The IC50 value of echinomycin was(22.7±7.36) nM and mimosine did not alter the parasite load in primary macrophages.The macrophage viability IC50 values for resveratrol,echinomycin and CdCl_2 and mimosine were >40 μM,>100 nM,> 200 μM and>2 000 μM,respectively.In vivo no differences between cutaneous lesions from control,resveratrol-and echinomycin-treated Balb/c mice were detected.Conclusions:Resveratrol,echinomycin and CdCl_2 reduce parasite survival in vitro.The HIF-1α targeting pharmacological drugs require further study to more fully determine their anti-Leishmania potential and their role in therapeutic strategies.展开更多
The Caesalpinioideae subfamily contains many well-known trees that are important for economic sustainability and human health,but a lack of genomic resources has hindered their breeding and utilization.Here,we present...The Caesalpinioideae subfamily contains many well-known trees that are important for economic sustainability and human health,but a lack of genomic resources has hindered their breeding and utilization.Here,we present chromosome-level reference genomes for the two food and industrial trees Gleditsia sinensis(921 Mb)and Biancaea sappan(872 Mb),the three shade and ornamental trees Albizia julibrissin(705 Mb),Delonix regia(580 Mb),and Acacia confusa(566 Mb),and the two pioneer and hedgerow trees Leucaena leucocephala(1338 Mb)and Mimosa bimucronata(641 Mb).Phylogenetic inference shows that the mimosoid clade has a much higher evolutionary rate than the other clades of Caesalpinioideae.Macrosynteny comparison suggests that the fusion and breakage of an unstable chromosome are responsible for the difference in basic chromosome number(13 or 14)for Caesalpinioideae.After an ancient whole-genome duplication(WGD)shared by all Caesalpinioideae species(CWGD,~72.0 million years ago[MYA]),there were two recent successive WGD events,LWGD-1(16.2-19.5 MYA)and LWGD-2(7.1-9.5 MYA),in L.leucocephala.Thereafter,~40%gene loss and genome-size contraction have occurred during the diploidization process in L.leucocephala.To investigate secondary metabolites,we identified all gene copies involved in mimosine metabolism in these species and found that the abundance of mimosine biosynthesis genes in L.leucocephala largely explains its high mimosine production.We also identified the set of all potential genes involved in triterpenoid saponin biosynthesis in G.sinensis,which is more complete than that based on previous transcriptome-derived unigenes.Our results and genomic resources will facilitate biological studies of Caesalpinioideae and promote the utilization of valuable secondary metabolites.展开更多
文摘A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 μ column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ± 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.
文摘A method is described to isolate mfinosine degrading bacteria from mmen juice from German steers which had never been ted on either leucaena or mimosine. The isolate was cultivated in continuous culture in a bench top fermenter in Medium 98-5 amended with an increasing amount of mimosine for 16 days. Degradation of mimosine was determined with a colorrinetfic method Incubated at 39 ℃ under anaerobic conditions, the isolate showed complete degradation of mimosine within one ~veek. The bacteriological pure isolate is an aero-tolerant gram-negative cocco-bacillus. By DNA sequencing analysis it belongs to genus Klebsiella. It was multiplied hi brain-heart-infusion, entrapped in alginate, and dried （37 ℃）. Feeding trials in sheep in Myalmlar showed complete innocuity＇ and complete detoxification of mimosine from leucaena leaves.
基金This work was supported by grants from APRI-ASANT and CIHR(MOP-84480)SK and from APRI(APRI 201600028)HW.SynAD,University of Alberta provided a part of the postdoctoral fellowships for BGA,QW and KG.
文摘Evidence suggests that increased level/aggregation of beta-amyloid(Aβ)peptides initiate neurodegeneration and subsequent development of Alzheimer’s disease(AD).At present,there is no effective treatment for AD.In this study,we reported the effects of gold nanoparticles surface-functionalized with a plant-based amino acid mimosine(Mimo-AuNPs),which is found to cross the blood-brain barrier,on the Aβfibrillization process and toxicity.Thioflavin T kinetic assays,fluorescence imaging and electron microscopy data showed that Mimo-AuNPs were able to suppress the spontaneous and seed-induced A_(1-42) aggregation.Spectroscopic studies,molecular docking and biochemical analyses further revealed that Mimo-AuNPs stabilize A_(1-42) to remain in its monomeric state by interacting with the hydrophobic domain of A_(1-42)(i.e.,Lys16 to Ala21)there by preventing a conformational shift towards theβ-sheet structure.Additionally,Mimo-AuNPs were found to trigger the disassembly of matured A_(1-42) fibers and increased neuronal viability by reducing phosphorylation of tau protein and the production of oxyradicals.Collectively,these results reveal that the surface-functionalization of gold nanoparticles with mimosine can attenuate Aβfibrillization and neuronal toxicity.Thus,we propose Mimo-AuNPs may be used as a potential treatment strategy towards AD-related pathologies.
文摘Mimosine, a plant amino acid, is a reversible cell cycle inhibitor. Biochemical studies have indicated that mimosine may act at multiple levels near the Gl/S interface. By using mi-croinjection technique, it is shown that mimosine can also inhibit the replication of Xenopus ribo-some DNA (rDNA) plasmid reversibly. The high structure similarity between mimosine
基金supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo,Conselho Nacional de Desenvolvimento Científico e Tecnologico (NO.2009/10771-9)Coordenacao de Aperfeicoamento de Pessoal de Nível Superior (NO.301052/2009-3),Brazil
文摘Objective:To evaluate whether hypoxia inducible factor(HIF-1α) targeting pharmacological drugs,echinomycin,resveratrol and CdCl_2 which inhibit HIF-1α stimulation,and mimosine,which enhances the stability of HIF-1α present antileishmanial properties.Methods:The leishmanicidal effect of drugs was evaluated in mouse macrophages and Balb/c mouse model for cutaneous leishmaniosis.Results:Resveratrol and CdCl_2 reduced the parasite load [IC50,(27.3±2.25) μM and(24.8±0.95) μM,respectively].The IC50 value of echinomycin was(22.7±7.36) nM and mimosine did not alter the parasite load in primary macrophages.The macrophage viability IC50 values for resveratrol,echinomycin and CdCl_2 and mimosine were >40 μM,>100 nM,> 200 μM and>2 000 μM,respectively.In vivo no differences between cutaneous lesions from control,resveratrol-and echinomycin-treated Balb/c mice were detected.Conclusions:Resveratrol,echinomycin and CdCl_2 reduce parasite survival in vitro.The HIF-1α targeting pharmacological drugs require further study to more fully determine their anti-Leishmania potential and their role in therapeutic strategies.
基金supported by the Shenzhen Science and Technology Program(JCYJ20190814163805604,KQTD20180411143628272)the Fund of Key Laboratory of Shenzhen(ZDSYS20141118170111640)The Agricultural Science and Technology Innovation Program.
文摘The Caesalpinioideae subfamily contains many well-known trees that are important for economic sustainability and human health,but a lack of genomic resources has hindered their breeding and utilization.Here,we present chromosome-level reference genomes for the two food and industrial trees Gleditsia sinensis(921 Mb)and Biancaea sappan(872 Mb),the three shade and ornamental trees Albizia julibrissin(705 Mb),Delonix regia(580 Mb),and Acacia confusa(566 Mb),and the two pioneer and hedgerow trees Leucaena leucocephala(1338 Mb)and Mimosa bimucronata(641 Mb).Phylogenetic inference shows that the mimosoid clade has a much higher evolutionary rate than the other clades of Caesalpinioideae.Macrosynteny comparison suggests that the fusion and breakage of an unstable chromosome are responsible for the difference in basic chromosome number(13 or 14)for Caesalpinioideae.After an ancient whole-genome duplication(WGD)shared by all Caesalpinioideae species(CWGD,~72.0 million years ago[MYA]),there were two recent successive WGD events,LWGD-1(16.2-19.5 MYA)and LWGD-2(7.1-9.5 MYA),in L.leucocephala.Thereafter,~40%gene loss and genome-size contraction have occurred during the diploidization process in L.leucocephala.To investigate secondary metabolites,we identified all gene copies involved in mimosine metabolism in these species and found that the abundance of mimosine biosynthesis genes in L.leucocephala largely explains its high mimosine production.We also identified the set of all potential genes involved in triterpenoid saponin biosynthesis in G.sinensis,which is more complete than that based on previous transcriptome-derived unigenes.Our results and genomic resources will facilitate biological studies of Caesalpinioideae and promote the utilization of valuable secondary metabolites.
