Mastitis is a complex, multifactorial disease. Pathogens, cows and farmers （via management） all play a role. It is costly and annoying for the farmer and threatens the image of the entire dairy industry. Prevention ...Mastitis is a complex, multifactorial disease. Pathogens, cows and farmers （via management） all play a role. It is costly and annoying for the farmer and threatens the image of the entire dairy industry. Prevention and control of mastitis is based on multiple principles that have been known for a long time. To implement them successfully, they should be put forward by a motivated and motivating advisor that transfers the existing knowledge to the farmer. When the changes are data-driven, applied by an encouraged farmer through a farm-specific implementation, prevention and control of mastitis will be successful and result in happy cows, happy farmers, happy advisors, happy consumers, and a happy industry. Nationwide projects focussing on communication and transfer of existing knowledge in prevention and control are very helpful in reaching high numbers of farmers and advisors and harmonizing the message brought by different parties. This paper gives an overview of multifactorial approach of mastitis management and prevention with a focus on milking, bedding and data-analysis.展开更多
This study was to supply the systemic and full milking process data to support the implementation of both dairy herd improvement （DHI） and digital feeding of dairy cattle. This study designed the relational structur...This study was to supply the systemic and full milking process data to support the implementation of both dairy herd improvement （DHI） and digital feeding of dairy cattle. This study designed the relational structured database and developed a set of digital management information system on milking process of intensive dairy farm using Visual Basic 6.0, Access databases, and Crystal report combining the milking characteristics of a grown cow, such as quality and sanitation testing indexes of raw milk. The system supplies a series of convenient, intelligent input interfaces of crude datum, and can count, analyze, and graphically show milking datum based on different types and different parities of cows or herds in a specific duration, and can dynamically produce some important derived data, such as days of grown cow, daily average of milk production of grown cow, days of cow milk production, and daily average of milking cow production; and can carry out all-pervasive data mining. With the help of system analysis and software design techniques, it is possible to realize precision farming for a dairy cattle herd based on whole digital management of milking process and realtime prediction on nutrient requirements and ration of dairy cattle, as well as dairy herd improvement.展开更多
The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein composi...The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein compositions were studied. The results showed thatβ-CN,κ-CN andα-La were monomorphic,αs1-CN andβ-Lg were polymorphic, the dominantgenes were αs1-CN D and β-Lg E,respectively. The frequencies of αs1-CN D were 0.8073and 0.6000 and β-Lg E were 0.9770 and 0.9700 in two populations respectively.The meanheterozygosities were 0.1021 and 0.1867 in two populations. No significant effects onmilking traits and milk protein compositions were observed except for αs1-CN locus onfat percentage in Jiulong yak.展开更多
Prolonged milk provisioning and extended parental care for nutritionally independent offspring, previously considered to only co-occur in long-lived mammals (Clutton-Brock, 1991;Royle et al., 2012), were recently repo...Prolonged milk provisioning and extended parental care for nutritionally independent offspring, previously considered to only co-occur in long-lived mammals (Clutton-Brock, 1991;Royle et al., 2012), were recently reported in the reproduction of the milking spider, Toxeus magnus (Chen et al. 2018). Newly hatched T. magnus spiderlings require 53 days to develop to maturity, with an average adult body length of 6.6 mm.展开更多
BACKGROUND Umbilical cord milking(UCM)is an alternative placental transfusion method for delayed umbilical cord clamping in routine obstetric practice,allowing prompt resuscitation of an infant.Thus,UCM has been adopt...BACKGROUND Umbilical cord milking(UCM)is an alternative placental transfusion method for delayed umbilical cord clamping in routine obstetric practice,allowing prompt resuscitation of an infant.Thus,UCM has been adopted at some tertiary neonatal centers for preterm infants to enhance placental-to-fetal transfusion.It is not suggested for babies less than 28 wk of gestational age because it is associated with severe brain hemorrhage.For late preterm or term infants who do not require resuscitation,cord management is recommended to increase iron levels and prevent the development of iron deficiency anemia,which is associated with impaired motor development,behavioral problems,and cognitive delays.Concerns remain about whether UCM increases the incidence of intraventricular hemorrhage.However,there are very few reports of late preterm infants presenting with neonatal hemorrhage stroke(NHS)and severe coagulopathy after receiving UCM.Here,we report a case of a late preterm infant born at 34 wk of gestation.She abruptly deteriorated,exhibiting signs and symptoms of NHS and severe coagulopathy after receiving UCM on the first day of life.CASE SUMMARY A female preterm infant born at 34 wk of gestation received UCM after birth.She was small for her gestational age and described as vigorous with Apgar scores of 9 and 10 at one minute and five minutes of life,respectively.After hospitalization in the neonatal intensive care unit,she showed hypoglycemia and metabolic acidosis.The baby was administered glucose and sodium bicarbonate infusions.Intramuscular vitamin K1 was also used to prevent vitamin K deficiency.The baby developed umbilical cord bleeding and gastric bleeding on day 1 of life;a physical examination showed bilateral conjunctival hemorrhage,and a blood test showed thrombocytopenia,prolonged prothrombin time,prolonged activated partial thromboplastin time,low fibrinogen,raised D-dimer levels and anemia.A subsequent cranial ultrasound and computed tomography scan showed a left parenchymal brain hemorrhage with extension into the ventricular and subarachnoid spaces.The patient was diagnosed with NHS in addition to disseminated intravascular coagulation(DIC).Fresh frozen plasma(FFP)and prothrombin complex concentrate were given for coagulopathy.Red blood cell and platelet transfusions were provided for thrombocytopenia and anemia.A bolus of midazolam,intravenous calcium and phenobarbital sodium were administered to control seizures.The baby’s clinical condition improved on day 5 of life,and the baby was hospitalized for 46 d and recovered well without seizure recurrence.Our case report suggests that preterm infants who receive UCM should undergo careful clinical assessment for intracranial hemorrhage,NHS and severe coagulopathy that may develop under certain circumstances.Supportive management,such as intensive care,FFP and blood transfusion,is recommended when the development of massive NHS and associated DIC is suspected.CONCLUSION Our case report suggests that for late preterm infants who are small for gestational age and who receive UCM for alternative placental transfusion,neonatal health care professionals should be cautious in assessing the development of NHS and severe coagulopathy.Neonatal health care professionals should also be more cautious in assessing the complications of late preterm infants after they receive UCM.展开更多
Background: Delayed cord clamping (DCC) and umbilical cord milking (UCM) are two recently advocated interventions to enhance placental transfusion at birth. DCC and MCM might increase blood volume and help neonates to...Background: Delayed cord clamping (DCC) and umbilical cord milking (UCM) are two recently advocated interventions to enhance placental transfusion at birth. DCC and MCM might increase blood volume and help neonates to transition from fetal to neonatal circulation. Objective: To evaluate the effects of delayed cord clamping (DCC), umbilical cord milking (UCM), and immediate cord clamping (ICC) on very low birth weight (VLBW) neonates. Methods: At our institution, ICC was routinely done until December, 2012 when it was replaced by DCC. UCM was implemented in March, 2015. Neonatal outcomes were compared among ICC, DCC, and UCM. Results: A total of 94 neonates met inclusion criteria. Comparing with ICC neonates, DCC and UCM neonates had higher hematocrits, fewer blood transfusions, and lower incidence of chronic lung disease at 36 completed weeks of gestation. All groups were similar in APGAR scores, vasopressor use, intraventricular hemorrhage (IVH) and necrotizing enterocolitis (NEC). Conclusion: Compared to ICC, DCC and UCM appear to be more beneficial to VLBW neonates without apparent adverse risks.展开更多
Conventional vacuum control in a milking system is accomplished by using a vacuum pump, sized for the maximum air flows into the milking system, running at a full speed. The difference between the pump capacity and th...Conventional vacuum control in a milking system is accomplished by using a vacuum pump, sized for the maximum air flows into the milking system, running at a full speed. The difference between the pump capacity and the necessary flow of air is compensated by allowing air to enter the system through a regulator. The solution presented in this paper uses a VFD （variable frequency driver） in order to drive the vacuum pump at a controlled speed, so that the air removed equals the air entering the milking system. The VFD technology is able to adjust the rate of air removal from the milking system, by changing the speed of the vacuum pump motor. The VFD is controlled by a computer using a virtual instrument in order to emulate a PID （proportion integration differentiation） regulator. The tests aimed to evaluate the vacuum regulator characteristics and vacuum stability. A statistical analysis of the experimental results was performed and it showed that there was a significant difference between the experimental results obtained for the two methods of vacuum regulation （with vacuum regulator and VFD controller respectively）. The experimental results proved that the used of the VFD controller led to a higher vacuum stability in terms of the error between the set vacuum value and the achieved values.展开更多
Wild yak males were crossed with domesticated female yaks to produce a breeding herd of crossbred animals. The milk production of the progeny of this herd was measured in the present study. Yaks were milked once daily...Wild yak males were crossed with domesticated female yaks to produce a breeding herd of crossbred animals. The milk production of the progeny of this herd was measured in the present study. Yaks were milked once daily after prior suckling of the calf. yaks were allowed to graze for 15 h/day on high-altitude(3200 to 3500 m) pastures. No supplementary food was given. Average daily milk yield of 1.77(s. e. 0. 16)kg and milk yield over 120 days,212.2(s. e. 20.2)kg were significantly higher(P＜0.01)than the corresponding figures for uninlproved domestic yaks(1.53(s. e. 0. 10)kg and 164. 6(s. e. 10.5)kg respectively). The fat content of milk from crossbred yaks, 52(s. e. 2. 9)g/kg, was not significantly dlfferent from that of unimproved animals, 53.5(s. e. 4. 1), g/kg.展开更多
Automatic milking systems(AMS) were designed to replace existing, labor-intensive machine milking and are an area of rapid development in modern dairy farming. The popularity of AMS lies in the convenience of manageme...Automatic milking systems(AMS) were designed to replace existing, labor-intensive machine milking and are an area of rapid development in modern dairy farming. The popularity of AMS lies in the convenience of management, decreasing workloads and the consistency of milking compared with non-automated machine milking. Nevertheless, this innovation has not been reviewed comprehensively and the practical benefits of AMS are still unclear. This review gives a brief overview of the historical development of milking machines and the workflow process of state-of-the-art AMS. In addition, a series of comparisons between AMS and current milking machines are made with respect to labor savings, quality parameters, udder health, herd behavior and mastitis detection and are summarized on the basis of relevant studies to show the benefits of the technological changes achieved by AMS. Finally, this review addresses several deficiencies in the technology and procedures of current AMS that need to be improved and also assesses recent advances in milking techniques with a particular focus on their potential for application in AMS.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic a...Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.展开更多
Background Nicotinamide riboside(NR)supplementation has been demonstrated efficacy in enhancing female reproductive outcomes,but its regulatory role in sow performance and gut microbiome remains undefined.This study s...Background Nicotinamide riboside(NR)supplementation has been demonstrated efficacy in enhancing female reproductive outcomes,but its regulatory role in sow performance and gut microbiome remains undefined.This study systematically evaluated the impacts of dietary NR supplementation during late gestation and lactation on sow performance and gut microbiome remodeling.A total of 280 sows were randomized assigned to one of four groups:a control group fed basal diet or one of three groups receiving NR-supplemented diets(2,4,or 8 g/d;n=70/group).Sow reproductive performance,blood metabolic parameters,milk metabolome,and fecal 16S rRNA sequencing were measured.Results Maternal NR supplementation linearly shortened farrowing duration(P<0.01)and tended to decrease the incidence of intrauterine growth restriction and the number of late gestation mummies(P<0.1),while concurrently increasing the within-litter uniformity(P=0.1).Litter weaning weight and average daily gain increased quadratically with NR dosage(P<0.05).NR supplementation orchestrated plasma metabolite regulation(triglycerides and total cholesterol;P<0.05),enhanced antioxidant biomarkers(T-AOC,GSH-Px,T-SOD;P<0.05),and reduced inflammatory cytokines(TNF-α;P<0.05)across gestation and lactation.Milk yield,colostrum/milk dry matter,crude protein,and crude fat were increased(P<0.05),together with higher levels of NAD^(+)metabolites(NAD^(+),NR,nicotinamide)and beneficial bioactive factors(milk polar lipids,3-aminosalicylic acid,fenugreekine;P<0.05).Gut microbiota analyses at lactation day 14 revealed NR-enriched beneficial taxa(Bifidobacterium,Ruminococcus,Lachnospiraceae,Subdoligranulum,Clostridium butyricum,Succiniclasticum)across sow-offspring dyads,which was associated with the activation of microbial NAD^(+)enzymes(NadR/NAMPT;P<0.05)and enhancement of systemic short-chain fatty acid flux,notably an increase in plasma butyrate acid(P<0.05).Conclusion Maternal supplementation of NR during late gestation and lactation increases sow performance and promotes gut NAD^(+)metabolic-associated microbiome remodeling.These findings propose maternal NR intervention as a novel strategy to enhance mammary lactogenesis and lactation metabolism in swine production,with potential applications for therapeutic strategies for lactation insufficiency.展开更多
Paying an additional RMB 2 could have your next milk tea delivered by drone to your balcony in just five minutes.This small fee represents the vast potential of the trillion-yuan lowaltitude economy.
Abuse of chloramphenicol(CAP)could cause serious side effects to human health.Therefore,it is necessary to detect CAP residues in animal sourced food effectively.Here,the superiority(better stability,higher Apt-CAP lo...Abuse of chloramphenicol(CAP)could cause serious side effects to human health.Therefore,it is necessary to detect CAP residues in animal sourced food effectively.Here,the superiority(better stability,higher Apt-CAP loading efficiency,and higher CAP binding associated conformational change,etc.)of tetrahedral structure to double-chained structure for developing aptasensor was evaluated.Then,a self-assembled DNA tetrahedral structure-based electrochemical aptasensor targeting CAP was developed.Under the optimized conditions,the aptasensor exhibited high sensitivity toward CAP with a limit of detection(LOD)of 0.0676 ng/m L(linear range 0.19-387.76 ng/m L),and high selectivity against the structural analogs of CAP.Moreover,the recovery rate of CAP from spiked milk samples ranged from 100.57%to 101.69%,and only USD 1.34 is needed for detecting CAP in 1 sample.These results suggested the application potential of this aptasesor for detecting CAP in animal sourced foods.展开更多
Background Exosomes are crucial mediators of intercellular communication.As a key component of milk,milkderived exosomes are abundant in genetic cargo,particularly micro RNAs(mi RNAs),indicating their potential role i...Background Exosomes are crucial mediators of intercellular communication.As a key component of milk,milkderived exosomes are abundant in genetic cargo,particularly micro RNAs(mi RNAs),indicating their potential role in regulating mammary gland physiology.Therefore,this study aimed to investigate the specificity of mi RNAs in milkderived exosomes and their regulatory roles in lipid synthesis in bovine mammary epithelial cells(BMECs).Results Based on 17,838 DHI records showing a significantly higher milk fat percentage(MFP)in late lactation(4.24%±1.07%),10 high-(5.96%±0.26%,HMF)and 10 low-MFP(1.68%±0.23%,LMF)cows were selected during this stage for milk-derived exosome isolation and mi RNA profiling.Exosomes isolated via differential ultracentrifugation were verified as 50-150 nm vesicles expressing CD9,CD81,and TSG101.mi RNA sequencing identified 1,320 differentially expressed mi RNAs(496 upregulated and 824 downregulated)between the HMF_EXO and LMF_EXO groups.Uptake assays confirmed that BMECs internalized these exosomes,and q RT-PCR validation showed that mi R-423-5p and mi R-125b were significantly upregulated and downregulated in HMF_EXO-and LMF_EXO-treated BMECs,respectively.Functionally,exosomal mi R-423-5p promoted intracellular lipid accumulation and TG synthesis in BMECs by targeting APOA5,whereas mi R-125b inhibited lipolysis and fatty acid oxidation by repressing SLC27A1.Conclusions This study demonstrates that milk-derived exosomal mi RNAs represent a novel mechanism for regulating milk fat synthesis.Specifically,mi R-423-5p and mi R-125b directly modulated lipid metabolism in BMECs via the mi R-423-5p/APOA5 and mi R-125b/SLC27A1 pathways.These findings provide new insights into the molecular regulation of milk fat synthesis and highlight the importance of exosome-mediated intercellular communication in the lactating mammary gland.展开更多
Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the pr...Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the prevention and treatment of AD.This study aimed to investigate the ameliorative effects of a new rice milk product fermented with Saccharomyces cerevisiae on AD mice.Fermented rice milk could effectively attenuated the symptoms of AD mice,such as skin lesions,scaliness and lichenification.Furthermore,fermented rice milk decreased the levels of inflammatory cytokines interleukin(IL)-4,IL-6 and tumor necrosis factor(TNF)-αin 2,4-dinitrochlorobenzene(DNCB)induced mice.It was also observed that fermented rice milk treatment downregulated the levels of p-IKBα,p-NF-κB,p-ERK,p-JNK and p-p38 proteins expression in skin of AD mice,which showed the anti-inflammatory function of fermented rice milk,mainly via the NF-κB and MAPK signaling pathways.In addition,S.cerevisiae fermentation reduced original flavor of rice milk,and increased the acid and alcohol compounds.Overall,the findings indicated that S.cerevisiae fermentation improved the flavor characteristics of rice milk,and fermented rice milk exhibited protective effects against DNCB-induced atopic dermatitis by downregulating NF-κB and MAPK signaling pathways and protecting the skin barrier.展开更多
Although human milk fat substitute(HMFS)has been wildly applied in infant formula(IF),there are still some differences in fat composition between human milk and IF,which is extremely attributed to the phospholipid com...Although human milk fat substitute(HMFS)has been wildly applied in infant formula(IF),there are still some differences in fat composition between human milk and IF,which is extremely attributed to the phospholipid composition.We therefore designed a human milk phospholipid substitute(HMPS),and investigated its nutritional outcomes on brain development using C57BL/6J mice.Results showed that HMPS improved mice's cognitive behavior and dendritic development compared to soybean phospholipids and nophospholipid diets.Based on the lipidomics,we revealed that these beneficial outcomes were potentially associated with the increased biosynthesis of N-acylethanolamines,phosphatidylethanolamine(PE),and ether PE,and the decreased metabolism of diacylglycerol and hexosylceramide in brain.It was further found in Kyoto Encyclopedia of Genes and Genomes analysis that glycerophospholipid metabolic pathway participated in the improvement of HMPS on mice's neurodevelopment.In conclusion,this study demonstrated that HMPS improves mice's neurodevelopment,and the glycerophospholipid metabolic pathway plays a major role in this beneficial result,and provided evidence for future application of HMPS in commercial IF.展开更多
Background This study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics.Eighteen dairy cows w...Background This study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics.Eighteen dairy cows were subjected to no heat stress(No-HS),mild heat stress(Mild-HS),and moderate heat stress(Mod-HS).Blood and milk samples were collected to determine the content and composition of amino acids(AA),and milk samples were used for metabolomic and proteomic analyses.Results Milk protein yield was significantly lower under Mild-HS and Mod-HS than No-HS(P<0.001).During Mild-HS,no significant difference was found in total AA concentration in both arterial(P=0.545)and venous blood(P=0.057),but arterial AA supply to the mammary gland significantly increased(P=0.045)when compared with No-HS.Under Mod-HS,the supply(P<0.001)and uptake(P=0.001)of total AA in the mammary gland decreased significantly,affecting the availability of precursors necessary for milk protein synthesis.Milk metabolomic analysis revealed that Mod-HS significantly impacted nucleotide metabolism,energy metabolism,and protein synthesis processes including translation,folding,and transport.Milk proteomic analysis showed significant downregulation of ribosomal and heat shock proteins which are crucial for protein synthesis and folding.Conclusions These findings suggest that heat stress disrupts AA utilization and energy metabolism in the mammary gland,leading to the reduced efficiency in milk protein synthesis and lowered milk protein yield.This study offers valuable insights into the metabolic and proteomic changes in dairy cows under heat stress,highlighting potential strategies to mitigate the adverse effects of heat stress on dairy production and milk quality.展开更多
Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,whi...Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,which were not well summarized.Here,we summarized these values in 9898 human breast milk samples of 6664 mothers from 50 countries in 81 studies.A literature search was conducted using PubMed,Embase,and Web of Science between January 1980 and October 2023.The 95%confidence interval of LA/ALA ratio across lactation and gestation ranged from 14.24 to 31.26.The LA content was higher in China and Turkey(>20%)whereas the ALA content was below 1%in Africa.The LA/ALA ratio in countries along the Mediterranean coast exceeded 20 or even 30.LA and ALA contents increased significantly(P<0.01)while the ratio remained stable over the last 40 years.Multivariate meta-regression results showed that regions significantly(P<0.01)determined the LA,ALA,and LA/ALA ratio.Especially,maternal diet could definitely explain the variation while the effects of gestational age,lactation period was not significant.Clinical trials demonstrated that decreasing the LA/ALA ratio increased docosahexaenoic acid(22:6 n-3,DHA)status,reduced arachidonic acid(20:4 n-6,AA)contents,exerted no effect on the visual function of infants,and reached no consensus on growth.The current review aims to provide an overview on the LA and ALA contents and their ratio in human breast milk to raise concern in infant formula.展开更多
Traditional Chinese medicine has long regarded flaxseed as a natural remedy for constipation.However,the efficacy or mechanism of flaxseed plant milk(FPM),a functional beverage derived from flaxseed,in alleviating con...Traditional Chinese medicine has long regarded flaxseed as a natural remedy for constipation.However,the efficacy or mechanism of flaxseed plant milk(FPM),a functional beverage derived from flaxseed,in alleviating constipation has not yet been clearly established.In this study,network pharmacology was combined with in vivo experiments to evaluate the anti-constipation activity of FPM on water restriction-induced constipation mouse model.Compared with the model group,administration of FPM(100 mg/kg and 200 mg/kg)significantly shortened the time to first black stool excretion,increased number of fecal pellets and water content of fecal pellets,and improved Small Intestinal Transit Rate(p<0.05).Histological analysis revealed that FPM(200 mg/kg)preserved colonic epithelial integrity,reduced lamina propria damage,and restored goblet cell populations.Serum biochemical assays showed that FPM decreased vasoactive intestinal peptide(VIP)levels and increased 5-hydroxytryptamine(5-HT)concentrations.Network pharmacology identified 214 overlapping targets between flaxseed bioactive compounds and constipation-related genes,with key hub targets including TP53,CASP3,TNF,and IL6.KEGG pathway enrichment suggested that the PI3K/AKT signaling pathway may serve as a central regulatory axis.Western blot further confirmed that p-PI3K and p-AKT protein levels in the colon were reduced in constipated mice,whereas FPM administration markedly restored their expression,indicating activation of the PI3K/AKT pathway.Overall,these findings demonstrate that FPM alleviates constipation by improving stool characteristics,enhancing intestinal motility,and maintaining mucosal integrity,potentially via modulation of the PI3K/AKT pathway.This study provides new insights into the gastrointestinal benefits of flaxseed-derived functional foods and supports their development as dietary supplements for intestinal health.展开更多
文摘Mastitis is a complex, multifactorial disease. Pathogens, cows and farmers （via management） all play a role. It is costly and annoying for the farmer and threatens the image of the entire dairy industry. Prevention and control of mastitis is based on multiple principles that have been known for a long time. To implement them successfully, they should be put forward by a motivated and motivating advisor that transfers the existing knowledge to the farmer. When the changes are data-driven, applied by an encouraged farmer through a farm-specific implementation, prevention and control of mastitis will be successful and result in happy cows, happy farmers, happy advisors, happy consumers, and a happy industry. Nationwide projects focussing on communication and transfer of existing knowledge in prevention and control are very helpful in reaching high numbers of farmers and advisors and harmonizing the message brought by different parties. This paper gives an overview of multifactorial approach of mastitis management and prevention with a focus on milking, bedding and data-analysis.
基金the National Key Technologies R&D Program of China during the 11th-Five-Year Plan period(2006BAD10A02-2)
文摘This study was to supply the systemic and full milking process data to support the implementation of both dairy herd improvement （DHI） and digital feeding of dairy cattle. This study designed the relational structured database and developed a set of digital management information system on milking process of intensive dairy farm using Visual Basic 6.0, Access databases, and Crystal report combining the milking characteristics of a grown cow, such as quality and sanitation testing indexes of raw milk. The system supplies a series of convenient, intelligent input interfaces of crude datum, and can count, analyze, and graphically show milking datum based on different types and different parities of cows or herds in a specific duration, and can dynamically produce some important derived data, such as days of grown cow, daily average of milk production of grown cow, days of cow milk production, and daily average of milking cow production; and can carry out all-pervasive data mining. With the help of system analysis and software design techniques, it is possible to realize precision farming for a dairy cattle herd based on whole digital management of milking process and realtime prediction on nutrient requirements and ration of dairy cattle, as well as dairy herd improvement.
基金supported by the Nationa1 Natura1 Science Foundation of China(39870607).
文摘The milk protein polymorphisms were typed by polyacrylamide gel electrophoresis (PAGE)from 109 Maiwa and 100 Jiulong yaks, and the relationships among milk protein polymorphisms,milking traits and milk protein compositions were studied. The results showed thatβ-CN,κ-CN andα-La were monomorphic,αs1-CN andβ-Lg were polymorphic, the dominantgenes were αs1-CN D and β-Lg E,respectively. The frequencies of αs1-CN D were 0.8073and 0.6000 and β-Lg E were 0.9770 and 0.9700 in two populations respectively.The meanheterozygosities were 0.1021 and 0.1867 in two populations. No significant effects onmilking traits and milk protein compositions were observed except for αs1-CN locus onfat percentage in Jiulong yak.
基金supported by the National Natural Science Foundation of China(30800121)
文摘Prolonged milk provisioning and extended parental care for nutritionally independent offspring, previously considered to only co-occur in long-lived mammals (Clutton-Brock, 1991;Royle et al., 2012), were recently reported in the reproduction of the milking spider, Toxeus magnus (Chen et al. 2018). Newly hatched T. magnus spiderlings require 53 days to develop to maturity, with an average adult body length of 6.6 mm.
基金Supported by Zhejiang Province Medical Science and Technology Foundation of China,No.2021PY057.
文摘BACKGROUND Umbilical cord milking(UCM)is an alternative placental transfusion method for delayed umbilical cord clamping in routine obstetric practice,allowing prompt resuscitation of an infant.Thus,UCM has been adopted at some tertiary neonatal centers for preterm infants to enhance placental-to-fetal transfusion.It is not suggested for babies less than 28 wk of gestational age because it is associated with severe brain hemorrhage.For late preterm or term infants who do not require resuscitation,cord management is recommended to increase iron levels and prevent the development of iron deficiency anemia,which is associated with impaired motor development,behavioral problems,and cognitive delays.Concerns remain about whether UCM increases the incidence of intraventricular hemorrhage.However,there are very few reports of late preterm infants presenting with neonatal hemorrhage stroke(NHS)and severe coagulopathy after receiving UCM.Here,we report a case of a late preterm infant born at 34 wk of gestation.She abruptly deteriorated,exhibiting signs and symptoms of NHS and severe coagulopathy after receiving UCM on the first day of life.CASE SUMMARY A female preterm infant born at 34 wk of gestation received UCM after birth.She was small for her gestational age and described as vigorous with Apgar scores of 9 and 10 at one minute and five minutes of life,respectively.After hospitalization in the neonatal intensive care unit,she showed hypoglycemia and metabolic acidosis.The baby was administered glucose and sodium bicarbonate infusions.Intramuscular vitamin K1 was also used to prevent vitamin K deficiency.The baby developed umbilical cord bleeding and gastric bleeding on day 1 of life;a physical examination showed bilateral conjunctival hemorrhage,and a blood test showed thrombocytopenia,prolonged prothrombin time,prolonged activated partial thromboplastin time,low fibrinogen,raised D-dimer levels and anemia.A subsequent cranial ultrasound and computed tomography scan showed a left parenchymal brain hemorrhage with extension into the ventricular and subarachnoid spaces.The patient was diagnosed with NHS in addition to disseminated intravascular coagulation(DIC).Fresh frozen plasma(FFP)and prothrombin complex concentrate were given for coagulopathy.Red blood cell and platelet transfusions were provided for thrombocytopenia and anemia.A bolus of midazolam,intravenous calcium and phenobarbital sodium were administered to control seizures.The baby’s clinical condition improved on day 5 of life,and the baby was hospitalized for 46 d and recovered well without seizure recurrence.Our case report suggests that preterm infants who receive UCM should undergo careful clinical assessment for intracranial hemorrhage,NHS and severe coagulopathy that may develop under certain circumstances.Supportive management,such as intensive care,FFP and blood transfusion,is recommended when the development of massive NHS and associated DIC is suspected.CONCLUSION Our case report suggests that for late preterm infants who are small for gestational age and who receive UCM for alternative placental transfusion,neonatal health care professionals should be cautious in assessing the development of NHS and severe coagulopathy.Neonatal health care professionals should also be more cautious in assessing the complications of late preterm infants after they receive UCM.
文摘Background: Delayed cord clamping (DCC) and umbilical cord milking (UCM) are two recently advocated interventions to enhance placental transfusion at birth. DCC and MCM might increase blood volume and help neonates to transition from fetal to neonatal circulation. Objective: To evaluate the effects of delayed cord clamping (DCC), umbilical cord milking (UCM), and immediate cord clamping (ICC) on very low birth weight (VLBW) neonates. Methods: At our institution, ICC was routinely done until December, 2012 when it was replaced by DCC. UCM was implemented in March, 2015. Neonatal outcomes were compared among ICC, DCC, and UCM. Results: A total of 94 neonates met inclusion criteria. Comparing with ICC neonates, DCC and UCM neonates had higher hematocrits, fewer blood transfusions, and lower incidence of chronic lung disease at 36 completed weeks of gestation. All groups were similar in APGAR scores, vasopressor use, intraventricular hemorrhage (IVH) and necrotizing enterocolitis (NEC). Conclusion: Compared to ICC, DCC and UCM appear to be more beneficial to VLBW neonates without apparent adverse risks.
文摘Conventional vacuum control in a milking system is accomplished by using a vacuum pump, sized for the maximum air flows into the milking system, running at a full speed. The difference between the pump capacity and the necessary flow of air is compensated by allowing air to enter the system through a regulator. The solution presented in this paper uses a VFD （variable frequency driver） in order to drive the vacuum pump at a controlled speed, so that the air removed equals the air entering the milking system. The VFD technology is able to adjust the rate of air removal from the milking system, by changing the speed of the vacuum pump motor. The VFD is controlled by a computer using a virtual instrument in order to emulate a PID （proportion integration differentiation） regulator. The tests aimed to evaluate the vacuum regulator characteristics and vacuum stability. A statistical analysis of the experimental results was performed and it showed that there was a significant difference between the experimental results obtained for the two methods of vacuum regulation （with vacuum regulator and VFD controller respectively）. The experimental results proved that the used of the VFD controller led to a higher vacuum stability in terms of the error between the set vacuum value and the achieved values.
文摘Wild yak males were crossed with domesticated female yaks to produce a breeding herd of crossbred animals. The milk production of the progeny of this herd was measured in the present study. Yaks were milked once daily after prior suckling of the calf. yaks were allowed to graze for 15 h/day on high-altitude(3200 to 3500 m) pastures. No supplementary food was given. Average daily milk yield of 1.77(s. e. 0. 16)kg and milk yield over 120 days,212.2(s. e. 20.2)kg were significantly higher(P＜0.01)than the corresponding figures for uninlproved domestic yaks(1.53(s. e. 0. 10)kg and 164. 6(s. e. 10.5)kg respectively). The fat content of milk from crossbred yaks, 52(s. e. 2. 9)g/kg, was not significantly dlfferent from that of unimproved animals, 53.5(s. e. 4. 1), g/kg.
基金supported financially by the China National Science and Technology Support Program (2012BAK08B04)
文摘Automatic milking systems(AMS) were designed to replace existing, labor-intensive machine milking and are an area of rapid development in modern dairy farming. The popularity of AMS lies in the convenience of management, decreasing workloads and the consistency of milking compared with non-automated machine milking. Nevertheless, this innovation has not been reviewed comprehensively and the practical benefits of AMS are still unclear. This review gives a brief overview of the historical development of milking machines and the workflow process of state-of-the-art AMS. In addition, a series of comparisons between AMS and current milking machines are made with respect to labor savings, quality parameters, udder health, herd behavior and mastitis detection and are summarized on the basis of relevant studies to show the benefits of the technological changes achieved by AMS. Finally, this review addresses several deficiencies in the technology and procedures of current AMS that need to be improved and also assesses recent advances in milking techniques with a particular focus on their potential for application in AMS.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
基金supported by the National Key Research and Development Program of China(2021YFD2100700).
文摘Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.
基金jointly supported by the National Key R&D Program of China(2023YFD1300804)the National Natural Science Foundation of China(32472948)the earmarked fund for China Agriculture Research System(CARS-35)。
文摘Background Nicotinamide riboside(NR)supplementation has been demonstrated efficacy in enhancing female reproductive outcomes,but its regulatory role in sow performance and gut microbiome remains undefined.This study systematically evaluated the impacts of dietary NR supplementation during late gestation and lactation on sow performance and gut microbiome remodeling.A total of 280 sows were randomized assigned to one of four groups:a control group fed basal diet or one of three groups receiving NR-supplemented diets(2,4,or 8 g/d;n=70/group).Sow reproductive performance,blood metabolic parameters,milk metabolome,and fecal 16S rRNA sequencing were measured.Results Maternal NR supplementation linearly shortened farrowing duration(P<0.01)and tended to decrease the incidence of intrauterine growth restriction and the number of late gestation mummies(P<0.1),while concurrently increasing the within-litter uniformity(P=0.1).Litter weaning weight and average daily gain increased quadratically with NR dosage(P<0.05).NR supplementation orchestrated plasma metabolite regulation(triglycerides and total cholesterol;P<0.05),enhanced antioxidant biomarkers(T-AOC,GSH-Px,T-SOD;P<0.05),and reduced inflammatory cytokines(TNF-α;P<0.05)across gestation and lactation.Milk yield,colostrum/milk dry matter,crude protein,and crude fat were increased(P<0.05),together with higher levels of NAD^(+)metabolites(NAD^(+),NR,nicotinamide)and beneficial bioactive factors(milk polar lipids,3-aminosalicylic acid,fenugreekine;P<0.05).Gut microbiota analyses at lactation day 14 revealed NR-enriched beneficial taxa(Bifidobacterium,Ruminococcus,Lachnospiraceae,Subdoligranulum,Clostridium butyricum,Succiniclasticum)across sow-offspring dyads,which was associated with the activation of microbial NAD^(+)enzymes(NadR/NAMPT;P<0.05)and enhancement of systemic short-chain fatty acid flux,notably an increase in plasma butyrate acid(P<0.05).Conclusion Maternal supplementation of NR during late gestation and lactation increases sow performance and promotes gut NAD^(+)metabolic-associated microbiome remodeling.These findings propose maternal NR intervention as a novel strategy to enhance mammary lactogenesis and lactation metabolism in swine production,with potential applications for therapeutic strategies for lactation insufficiency.
文摘Paying an additional RMB 2 could have your next milk tea delivered by drone to your balcony in just five minutes.This small fee represents the vast potential of the trillion-yuan lowaltitude economy.
基金supported by the National Nature Science Foundation of China(82174531)the Medicine and Health Science Technology Development Project of Shandong Province,China(202212060626).
文摘Abuse of chloramphenicol(CAP)could cause serious side effects to human health.Therefore,it is necessary to detect CAP residues in animal sourced food effectively.Here,the superiority(better stability,higher Apt-CAP loading efficiency,and higher CAP binding associated conformational change,etc.)of tetrahedral structure to double-chained structure for developing aptasensor was evaluated.Then,a self-assembled DNA tetrahedral structure-based electrochemical aptasensor targeting CAP was developed.Under the optimized conditions,the aptasensor exhibited high sensitivity toward CAP with a limit of detection(LOD)of 0.0676 ng/m L(linear range 0.19-387.76 ng/m L),and high selectivity against the structural analogs of CAP.Moreover,the recovery rate of CAP from spiked milk samples ranged from 100.57%to 101.69%,and only USD 1.34 is needed for detecting CAP in 1 sample.These results suggested the application potential of this aptasesor for detecting CAP in animal sourced foods.
基金supported by the National Natural Science Foundation of China(no.32372838,U22A20506)the National Key Research and Development Program of China(no.2024YFD1300104)+1 种基金the scientific and technological development program of Jilin province(YDZJ202203CGZH037)the earmarked fund for JLARS-2025-070203。
文摘Background Exosomes are crucial mediators of intercellular communication.As a key component of milk,milkderived exosomes are abundant in genetic cargo,particularly micro RNAs(mi RNAs),indicating their potential role in regulating mammary gland physiology.Therefore,this study aimed to investigate the specificity of mi RNAs in milkderived exosomes and their regulatory roles in lipid synthesis in bovine mammary epithelial cells(BMECs).Results Based on 17,838 DHI records showing a significantly higher milk fat percentage(MFP)in late lactation(4.24%±1.07%),10 high-(5.96%±0.26%,HMF)and 10 low-MFP(1.68%±0.23%,LMF)cows were selected during this stage for milk-derived exosome isolation and mi RNA profiling.Exosomes isolated via differential ultracentrifugation were verified as 50-150 nm vesicles expressing CD9,CD81,and TSG101.mi RNA sequencing identified 1,320 differentially expressed mi RNAs(496 upregulated and 824 downregulated)between the HMF_EXO and LMF_EXO groups.Uptake assays confirmed that BMECs internalized these exosomes,and q RT-PCR validation showed that mi R-423-5p and mi R-125b were significantly upregulated and downregulated in HMF_EXO-and LMF_EXO-treated BMECs,respectively.Functionally,exosomal mi R-423-5p promoted intracellular lipid accumulation and TG synthesis in BMECs by targeting APOA5,whereas mi R-125b inhibited lipolysis and fatty acid oxidation by repressing SLC27A1.Conclusions This study demonstrates that milk-derived exosomal mi RNAs represent a novel mechanism for regulating milk fat synthesis.Specifically,mi R-423-5p and mi R-125b directly modulated lipid metabolism in BMECs via the mi R-423-5p/APOA5 and mi R-125b/SLC27A1 pathways.These findings provide new insights into the molecular regulation of milk fat synthesis and highlight the importance of exosome-mediated intercellular communication in the lactating mammary gland.
基金financial support received from the Shanghai Oriental Talent Youth Project(T2023218)the National Science Fund for Distinguished Young Scholars of China(32025029)+2 种基金the Young Elite Scientist Sponsorship Program of China Association for Science and Technology(YESS20220128)the Shanghai Research Center for Food Microbiology Engineering(19DZ2281100)the National Natural Science Foundation of China(Key Program)(U23A20261).
文摘Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the prevention and treatment of AD.This study aimed to investigate the ameliorative effects of a new rice milk product fermented with Saccharomyces cerevisiae on AD mice.Fermented rice milk could effectively attenuated the symptoms of AD mice,such as skin lesions,scaliness and lichenification.Furthermore,fermented rice milk decreased the levels of inflammatory cytokines interleukin(IL)-4,IL-6 and tumor necrosis factor(TNF)-αin 2,4-dinitrochlorobenzene(DNCB)induced mice.It was also observed that fermented rice milk treatment downregulated the levels of p-IKBα,p-NF-κB,p-ERK,p-JNK and p-p38 proteins expression in skin of AD mice,which showed the anti-inflammatory function of fermented rice milk,mainly via the NF-κB and MAPK signaling pathways.In addition,S.cerevisiae fermentation reduced original flavor of rice milk,and increased the acid and alcohol compounds.Overall,the findings indicated that S.cerevisiae fermentation improved the flavor characteristics of rice milk,and fermented rice milk exhibited protective effects against DNCB-induced atopic dermatitis by downregulating NF-κB and MAPK signaling pathways and protecting the skin barrier.
基金supported by Huhhot Science&Technology Plan(2021-National Center of Technology innovation for Dairy-4)Innovation Consortium Project(2023RC Consortium-7)+3 种基金National Key Research and Development Program(2023YFF1104502)Ganpo Juncai supports the plan(20232BCJ22046)Nanchang University Jiangxi Financial Science and Technology Special Project(ZBG20230418011)Free Exploration Project of State Key Laboratory(SKLF-ZZB-202317).
文摘Although human milk fat substitute(HMFS)has been wildly applied in infant formula(IF),there are still some differences in fat composition between human milk and IF,which is extremely attributed to the phospholipid composition.We therefore designed a human milk phospholipid substitute(HMPS),and investigated its nutritional outcomes on brain development using C57BL/6J mice.Results showed that HMPS improved mice's cognitive behavior and dendritic development compared to soybean phospholipids and nophospholipid diets.Based on the lipidomics,we revealed that these beneficial outcomes were potentially associated with the increased biosynthesis of N-acylethanolamines,phosphatidylethanolamine(PE),and ether PE,and the decreased metabolism of diacylglycerol and hexosylceramide in brain.It was further found in Kyoto Encyclopedia of Genes and Genomes analysis that glycerophospholipid metabolic pathway participated in the improvement of HMPS on mice's neurodevelopment.In conclusion,this study demonstrated that HMPS improves mice's neurodevelopment,and the glycerophospholipid metabolic pathway plays a major role in this beneficial result,and provided evidence for future application of HMPS in commercial IF.
基金funded by grants from the National Nature Science Foundation(Grant number:U23A20231)。
文摘Background This study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics.Eighteen dairy cows were subjected to no heat stress(No-HS),mild heat stress(Mild-HS),and moderate heat stress(Mod-HS).Blood and milk samples were collected to determine the content and composition of amino acids(AA),and milk samples were used for metabolomic and proteomic analyses.Results Milk protein yield was significantly lower under Mild-HS and Mod-HS than No-HS(P<0.001).During Mild-HS,no significant difference was found in total AA concentration in both arterial(P=0.545)and venous blood(P=0.057),but arterial AA supply to the mammary gland significantly increased(P=0.045)when compared with No-HS.Under Mod-HS,the supply(P<0.001)and uptake(P=0.001)of total AA in the mammary gland decreased significantly,affecting the availability of precursors necessary for milk protein synthesis.Milk metabolomic analysis revealed that Mod-HS significantly impacted nucleotide metabolism,energy metabolism,and protein synthesis processes including translation,folding,and transport.Milk proteomic analysis showed significant downregulation of ribosomal and heat shock proteins which are crucial for protein synthesis and folding.Conclusions These findings suggest that heat stress disrupts AA utilization and energy metabolism in the mammary gland,leading to the reduced efficiency in milk protein synthesis and lowered milk protein yield.This study offers valuable insights into the metabolic and proteomic changes in dairy cows under heat stress,highlighting potential strategies to mitigate the adverse effects of heat stress on dairy production and milk quality.
基金supported by National Key R&D Program of China(2021YFD2100700).
文摘Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,which were not well summarized.Here,we summarized these values in 9898 human breast milk samples of 6664 mothers from 50 countries in 81 studies.A literature search was conducted using PubMed,Embase,and Web of Science between January 1980 and October 2023.The 95%confidence interval of LA/ALA ratio across lactation and gestation ranged from 14.24 to 31.26.The LA content was higher in China and Turkey(>20%)whereas the ALA content was below 1%in Africa.The LA/ALA ratio in countries along the Mediterranean coast exceeded 20 or even 30.LA and ALA contents increased significantly(P<0.01)while the ratio remained stable over the last 40 years.Multivariate meta-regression results showed that regions significantly(P<0.01)determined the LA,ALA,and LA/ALA ratio.Especially,maternal diet could definitely explain the variation while the effects of gestational age,lactation period was not significant.Clinical trials demonstrated that decreasing the LA/ALA ratio increased docosahexaenoic acid(22:6 n-3,DHA)status,reduced arachidonic acid(20:4 n-6,AA)contents,exerted no effect on the visual function of infants,and reached no consensus on growth.The current review aims to provide an overview on the LA and ALA contents and their ratio in human breast milk to raise concern in infant formula.
基金supported by the Earmarked Fund for China Agricultural Research System(CARS-14)the Innovation Group Project of Hubei Province(2023AFA042)the Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI)。
文摘Traditional Chinese medicine has long regarded flaxseed as a natural remedy for constipation.However,the efficacy or mechanism of flaxseed plant milk(FPM),a functional beverage derived from flaxseed,in alleviating constipation has not yet been clearly established.In this study,network pharmacology was combined with in vivo experiments to evaluate the anti-constipation activity of FPM on water restriction-induced constipation mouse model.Compared with the model group,administration of FPM(100 mg/kg and 200 mg/kg)significantly shortened the time to first black stool excretion,increased number of fecal pellets and water content of fecal pellets,and improved Small Intestinal Transit Rate(p<0.05).Histological analysis revealed that FPM(200 mg/kg)preserved colonic epithelial integrity,reduced lamina propria damage,and restored goblet cell populations.Serum biochemical assays showed that FPM decreased vasoactive intestinal peptide(VIP)levels and increased 5-hydroxytryptamine(5-HT)concentrations.Network pharmacology identified 214 overlapping targets between flaxseed bioactive compounds and constipation-related genes,with key hub targets including TP53,CASP3,TNF,and IL6.KEGG pathway enrichment suggested that the PI3K/AKT signaling pathway may serve as a central regulatory axis.Western blot further confirmed that p-PI3K and p-AKT protein levels in the colon were reduced in constipated mice,whereas FPM administration markedly restored their expression,indicating activation of the PI3K/AKT pathway.Overall,these findings demonstrate that FPM alleviates constipation by improving stool characteristics,enhancing intestinal motility,and maintaining mucosal integrity,potentially via modulation of the PI3K/AKT pathway.This study provides new insights into the gastrointestinal benefits of flaxseed-derived functional foods and supports their development as dietary supplements for intestinal health.
