BACKGROUND Human milk oligosaccharides(HMOs)are bioactive components of breast milk with diverse health benefits,including shaping the gut microbiota,modulating the immune system,and protecting against infections.HMOs...BACKGROUND Human milk oligosaccharides(HMOs)are bioactive components of breast milk with diverse health benefits,including shaping the gut microbiota,modulating the immune system,and protecting against infections.HMOs exhibit dynamic secretion patterns during lactation,influenced by maternal genetics and environ-mental factors.Their direct and indirect antimicrobial properties have garnered significant research interest.However,a comprehensive understanding of the secretion dynamics of HMOs and their correlation with antimicrobial efficacy remains underexplored.AIM To synthesize current evidence on the secretion dynamics of HMOs during lactation and evaluate their antimicrobial roles against bacterial,viral,and protozoal pathogens.METHODS A systematic search of PubMed,Scopus,Web of Science,and Cochrane Library focused on studies investigating natural and synthetic HMOs,their secretion dynamics,and antimicrobial properties.Studies involving human,animal,and in vitro models were included.Data on HMO composition,temporal secretion patterns,and mechanisms of antimicrobial action were extracted.Quality assess-ment was performed using validated tools appropriate for study design.RESULTS A total of 44 studies were included,encompassing human,animal,and in vitro research.HMOs exhibited dynamic secretion patterns,with 2′-fucosyllactose(2′-FL)and lacto-N-tetraose peaking in early lactation and declining over time,while 3-fucosyllactose(3-FL)increased during later stages.HMOs demonstrated significant antimicrobial properties through pathogen adhesion inhibition,biofilm disruption,and enzymatic activity impairment.Synthetic HMOs,including bioengineered 2′-FL and 3-FL,were structurally and functionally comparable to natural HMOs,effectively inhibiting pathogens such as Pseudomonas aeruginosa,Escherichia coli,and Campylobacter jejuni.Additionally,HMOs exhibited synergistic effects with antibiotics,enhancing their efficacy against resistant pathogens.CONCLUSION HMOs are vital in antimicrobial defense,supporting infant health by targeting various pathogens.Both natural and synthetic HMOs hold significant potential for therapeutic applications,particularly in infant nutrition and as adjuncts to antibiotics.Further research,including clinical trials,is essential to address gaps in knowledge,validate findings,and explore the broader applicability of HMOs in improving maternal and neonatal health.展开更多
Background Milk synthesis is an energy-intensive process influenced by oxygen availability.This study investigates how hypoxia affects milk synthesis in BMECs,focusing on key genes involved in lactation and energy met...Background Milk synthesis is an energy-intensive process influenced by oxygen availability.This study investigates how hypoxia affects milk synthesis in BMECs,focusing on key genes involved in lactation and energy metabolism.Methods BMECs were cultured in a normoxic environment and then transferred to a hypoxia chamber with 1%O2 for specified durations.The study evaluated cellular responses through various molecular experiments and RNA sequencing.Small interfering RNA was employed to knock down HIF-1αto investigate whether the lactation-related phenotype alteration depends on HIF-1α.Results Hypoxia disrupted milk protein production by reducing mTOR/P70S6K/4EBP1 signaling and downregulating genes critical for amino acid transport and protein synthesis.Triglyceride synthesis increased due to enhanced fatty acid uptake and the upregulation of regulatory proteins,including FASN and PPARγ.Although glucose uptake was elevated under hypoxia,key enzymes for lactose synthesis were downregulated,suggesting a redirection of glucose toward energy production.Mitochondrial function was impaired under hypoxia,with reduced gene expression in TCA cycle,ETC,cytosol-mitochondrial transport,decreased ATP levels,increased ROS levels,and structural alterations.Additionally,lipid synthesis and glucose uptake depend on HIF-1α,while milk protein synthesis alterations occurred independently of HIF-1α.Conclusions Hypoxia alters milk synthesis in BMECs by disrupting milk protein synthesis,enhancing lipid metabolism,and impairing energy production.These findings provide valuable insights into the molecular mechanisms underlying the effect of oxygen deprivation on lactation efficiency,offering potential targets for mitigating hypoxic stress in the mammary glands of dairy animals.展开更多
BACKGROUND Although breast milk is ideal for newborns,in some cases,it is replaced with cow’s milk,which contains proteins that increase the risk of cow’s milk protein allergy(CMPA).AIM To evaluate CMPA prevalence i...BACKGROUND Although breast milk is ideal for newborns,in some cases,it is replaced with cow’s milk,which contains proteins that increase the risk of cow’s milk protein allergy(CMPA).AIM To evaluate CMPA prevalence in Bahrain and compare clinical characteristics of children with immunoglobulin E(IgE)-and non-IgE-mediated CMPA.METHODS This retrospective cohort study examined children with CMPA diagnosed at the pediatric gastroenterology outpatient clinic of the Salmaniya Medical Complex,Bahrain,between 2014 and 2022,and assessed CMPA prevalence.Clinical presentations,laboratory findings,dietary modifications,and outcomes were compared between children with IgE-mediated and non-IgE-mediated CMPA.Categorical variables were compared using Fisher’s exact test or Pearson’sχ2 test,whereas continuous variables were compared using Student’s t-test or the Mann-Whitney U test.RESULTS Of 8332 patients,6022(72.3%)adhered to their appointment.Of them,618(10.3%)were suggested of having CMPA and 595(96.3%)were included;CMPA prevalence was 2%.Most patients were Bahraini(93.8%)and males(55.3%).Non-IgE-mediated type accounted for 77.3%cases and IgE-mediated type,22.7%.IgE-mediated CMPA presented more in boys(P=0.030),and later in life(5.1 months±4.5 months vs 4.2 months±4.2 months,P=0.016,95%CI:0.08-1.73),had more associated diseases(P<0.001);and presented with more cutaneous(P=0.024)and respiratory(P=0.003)manifestations,severe symptoms[rash/dry skin(P=0.031),facial swelling/angioedema(P=0.003),failure to thrive(P=0.013),apparent life-threatening event(P<0.001)],and positive physical findings(P=0.002)than non-IgE-mediated CMPA.Most patients were exclusively fed cow milk formula(50.3%).The amino acid-based formula(AAF)was most prescribed(60.5%)with no difference between the two types(P=0.173).Although breastfeeding was recommended to 49.6%,only 8.2%were exclusively breastfed.IgE-mediated CMPA was associated with a longer follow-up duration than non-IgE-mediated CMPA(17.3 months±14.0 months vs 13.5 months±13.4 months,P=0.005,95%CI:1.1-6.3).CONCLUSION This study revealed a high CMPA prevalence with clinical differences between both types that can influence treatment.AAF was most prescribed,while breastfeeding with dietary modification is rarely applied.展开更多
Breast milk represents the gold standard for neonatal nutrition,especially for preterm and term infants with a low birthweight.This awareness is based not only on the nutritional properties of human milk,which is spec...Breast milk represents the gold standard for neonatal nutrition,especially for preterm and term infants with a low birthweight.This awareness is based not only on the nutritional properties of human milk,which is specifically designed for the growth of humans but also on breast milk’s non-nutritional properties,such as protection against infection.In fact,breast milk should be considered a heterogeneous ecosystem,including a wide range of cells in addition to those involved in immune function;growth factors,such as vascular endothelial growth factor;multiple noncoding microRNAs;immune cells;epithelial cells and multipotent mesenchymal stem cells.This recent identification of a pool of progenitor stem cells in human milk is the driving force behind the growing research aimed at identifying the nature of these stem/progenitor cells and their sources.展开更多
Background Lactational performance depends heavily on age,health,and nutrition.L-Citrulline(Cit)is an effective precursor of L-arginine(Arg),an amino acid that has important roles in synthesis of nitric oxide(NO)and p...Background Lactational performance depends heavily on age,health,and nutrition.L-Citrulline(Cit)is an effective precursor of L-arginine(Arg),an amino acid that has important roles in synthesis of nitric oxide(NO)and polyamines.Ruminal microbes degrade extracellular Arg;however,extracellular L-citrulline(Cit)is not degraded by ruminal microbes due to lack of uptake and can be fed unencapsulated as a precursor for Arg.As NO is a vasodilator,an increase in blood flow and transport of molecules to mammary tissue may enhance lactational performance and milk composition.Increases in polyamine production may increase milk protein synthesis within mammary tissue,thus increasing milk protein content.This study determined,for the first time,effects of dietary Cit supplementation on milk production and milk composition of Alpine dairy goats.Methods Does were synchronized to estrus and bred to Alpine bucks.Parturition was induced on d 149 of gestation and does were suckled overnight allowing kid(s)to obtain colostrum before being milked 24 h later(d 1 of lactation).Does were assigned to either control(CON,n=24)or Cit(CIT,n=23)diets.The isonitrogenous control diet consisted of 97.63%basal diet and 2.37%supplement(1.37%L-alanine and 1.00%soybean hydrogenated oil).The CIT supplemented diet consisted of 97.63%basal diet and 2.37%supplement(0.5%Cit,0.5%L-glutamine,1%soybean hydrogenated oil,0.37%cornstarch).Diets were group fed ad-libitum by treatment group.Blood samples were collected on d 0 and 30 of lactation,milk volumes measured twice daily,and on d 10,20,and 40 of lactation,milk samples were collected.Results CIT-treated does had greater daily milk production(P<0.05)and there was an effect of day of lactation on daily milk production(P<0.0001).Sire had significant effect on daily milk production as well(P<0.05).Milk compositional analyses revealed Cit supplementation increased solid-non-fat(SNF;P<0.05)and protein(P<0.05)content in milk.Conclusions Our novel results indicate that dietary supplementation of Cit fed ad-libitum in Alpine does increased daily milk yield,milk SNF content,and protein content.Supplemental Cit may be a proxy for Arg in goats to enhance lactational performance.展开更多
BACKGROUND Health maintenance in elderly houses includes management of the gut microbiota and the environment.Lacticaseibacillus paracasei Shirota(LcS)is a probiotic strain that positively affects the human gut.Howeve...BACKGROUND Health maintenance in elderly houses includes management of the gut microbiota and the environment.Lacticaseibacillus paracasei Shirota(LcS)is a probiotic strain that positively affects the human gut.However,the evidence of its effects on the Indonesian population remains limited.AIM To investigate the effect of LcS-fermented milk on the gut microbiota and environment of Indonesian elderly houses.METHODS This double-blind,randomized,placebo-controlled trial involved 112 participants from Indonesian elderly houses,spanning a 2-week baseline and 24-week treatment.Participants were randomly assigned to probiotic or placebo groups,consuming fermented milk with or without LcS(>6.5×10^(9)colony-forming units).Fecal samples were collected every three months.Gut microbiota analysis was performed using 16S rRNA gene sequencing and reverse transcription quantitative polymerase chain reaction,while gut environment was assessed by measuring fecal organic acids,amino acid metabolites,and stool frequency.RESULTS Analyses of 16S rRNA gene sequence data at the 3-month period revealed increased Bifidobacterium and Succinivibrio and decreased Rikenellaceae RC9 gut group in the probiotic group.These shifts were associated with significant differences inβ-diversity metrics.The change in Bifidobacterium was confirmed by reverse transcription quantitative polymerase chain reaction,demonstrating higher abundance in the probiotic group than in the placebo group(8.5±1.1 vs 8.0±1.1,log10 bacterial cells/g;P=0.044).At 6-month period,the differences in Succinivibrio and Rikenellaceae RC9 gut group persisted.The probiotic group showed higher butyrate levels than the placebo group at the 6-month period(5.04±3.11 vs 3.95±2.89,μmol/g;P=0.048).The effect on amino acid metabolites and stool frequency was not significant.CONCLUSION Daily intake of LcS positively affects the gut microbiota and environment of people living in Indonesian elderly houses.展开更多
Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigest...Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigestible lactose and allergenic proteins.Coconut milk has been around for a long time,but its application is limited due to a perceived lack of specific nutrients,high saturated fat levels,and low acceptability.Recent evidence indicates,however,that the saturated fat and other plant-based components found in coconut milk are good for metabolic outcomes and brain health.The conversion of coconut milk to yoghurt will further improve its functionality by boosting its existing nutritional qualities.In this article,the nutritional value of coconut milk,as well as its potential downsides,its application as yoghurt,and suggestions for enhancing its nutritional functionality will be examined.展开更多
Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced...Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced intestinal disorders,and compared the regulation patterns associated with gut microbiome and metabolome.The results showed plant-based milk alleviated the ceftriaxone caused cecum swelling,colonic tissue damage and intestinal microecological disorders.Meanwhile,administered plant-based milk decreased levels of pro-inflammatory cytokine(tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and oxidative stresses(malondialdehyde(MDA)and myeloperoxidase(MPO)in the colon,as well as increasing the levels of tight junction proteins(Occludin,Claudin-1,and ZO-1)in the colon.Moreover,administration of plant-based milk modulated the intestinal microbiota by promoting the relative levels of beneficial bacteria(Bifidobacterium),and inhibiting the harmful bacteria genus(Enterococcus).Furthermore,plant-based milk treatment significantly modulated glycerophospholipids metabolism(e.g.glycerophosphocholine)and arachidonic acid metabolism(e.g.prostaglandin G2 and arachidonic acid)in the serum.In conclusion,plant-based milk could alleviate antibiotic-related imbalance of barrier function damage,gut microbiota disorders and the reduction of metabolic disorders,which lays a foundation for exploring anti-inflammatory and intestinal micro-ecological approach to plant-based milk.展开更多
Dynamic changes in the physiochemical,structural,and flavor characteristics of ginger-juice milk curd were explored by texture analysis,scanning electron microscopy,rheometry,electronic tongue,and gas chromatography-m...Dynamic changes in the physiochemical,structural,and flavor characteristics of ginger-juice milk curd were explored by texture analysis,scanning electron microscopy,rheometry,electronic tongue,and gas chromatography-mass spectrometry(GC-MS).Protein electrophoresis showed that ginger juice could hydrolyzeαs-,β-,andκ-casein.Curd formation was initiated at 90 s,marked by significant changes in intensity detected via intrinsic fluorescence.The contents of soluble protein and calcium decreased rapidly during coagulation,while the caseinolytic activity,storage moduli,loss moduli,hardness,adhesiveness,and water-holding capacity increased,resulting in a denser gel structure with smaller pores and fewer cavitations as observed by scanning electron microscopy.Electronic tongue analysis indicated that milk could neutralize the astringency and saltiness of ginger juice,rendering the taste of ginger-juice milk curd more akin to that of milk.Approximately 70 volatile components were detected in ginger-juice milk curd.α-Zingiberene,α-curcumene,β-sesquiphellandrene,andβ-bisabolene were the predominant volatile flavor compounds,exhibiting an initial decrease in content followed by stability after 90 s.Decanoic acid,γ-elemene,and caryophyllene were identified as unique volatile compounds after mixing of milk and ginger juice.Understanding the dynamic changes in these characteristics during coagulation holds significant importance for the production of ginger-juice milk curd.展开更多
oil aggregates profoundly impact soil sustainability and crop productivity, and they are influenced by complexinteractions between minerals and organics. This study aimed to elucidate the alterations in mineralogy and...oil aggregates profoundly impact soil sustainability and crop productivity, and they are influenced by complexinteractions between minerals and organics. This study aimed to elucidate the alterations in mineralogy and soilorganic carbon(SOC) following long-term green manure incorporation and the effect on soil aggregates. Based on 5-and 36-year field experiments, surface soil samples(0–20 cm) were collected from Alfisol and Ferrisol soilssubjected to rice–rice–winter fallow(CK) and rice–rice–Chinese milk vetch(MV) treatments to investigate aggregatestability, mineralogy, SOC composition, and soil microstructural characteristics. The results showed that high clay-content Ferrisol exhibited greater aggregate stability than low clay-content Alfisol. The phyllosilicates in Alfisolprimarily comprised illite and vermiculite, whereas those in Ferrisol with high-content free-form Fe oxides(Fed) weredominated by kaolinite. Additionally, the clay fraction in Ferrisol contained more aromatic-C than the clay fraction inAlfisol. The 36-year MV incorporation significantly increased the Ferrisol macroaggregate stability(9.57–13.37%),and it also facilitated the transformation of vermiculite into kaolinite and significantly increased the clay, Fed, and aromatic-C contents in Ferrisol. Backscattered electron(BSE)-scanning electron microscopy/energy dispersive X-ray spectroscopy(SEM/EDS) revealed a compact aggregate structure in Ferrisol with co-localization of Feoxides and kaolinite. Moreover, the partial least path model(PLS-PM) revealed that clay content directly improvedmacroaggregate stability, and that kaolinite and Fed positively and directly affected clay or indirectly modulated clay formation by increasing the aromatic-C levels. Overall, long-term MV incorporation promotes clay aggregation by affecting mineral transformation to produce more kaolinite and Fe oxides and retain aromatic-C, and it ultimately improves aggregate stability.展开更多
Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted...Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted among 60 primi postnatal mothers(30 in each group).Study was conducted at the Postnatal ward of IMS and SUM Hospital,Bhubaneswar,Odisha.Tools used were structured sociodemographic pro forma,structured breast milk adequacy questionnaire,and structured rating scale to assess the adequacy of breast milk production.The duration of the study is 1 year.All the postnatal mothers who were primigravida and undergone cesarean section were chosen as the sample through a convenience sampling technique.Then,the samples were assigned to the intervention group(receiving neck massage)and control group(receiving standard postnatal care).The data were collected and analyzed using both descriptive and inferential statistics by SPSS version 20.Results:The study resulted a significant difference between the pretest and posttest score of breast milk production after neck massage(P=0.000).The mean score of the posttest(2.90±0.36)is more than the mean score of the pretest(2.89±0.36)of the baby’s weight(P<0.001).Further,the number of urination and stool passed in 24 h was more in the intervention group than the control group(P<0.001).The Chi-square analysis showed statistical significance between breast milk production after neck massage with religion of the postnatal mothers(P<0.05).Conclusion:The neck massage is found effective in improving the breast milk production among primi c-section mothers with improve newborn parameters.展开更多
Microbial-Induced Carbonate Precipitation(MICP)is an emerging,environmental-friendly,and sustainable technology that has shown great potential for soil stabilization.However,its process efficiency has been re-cognized...Microbial-Induced Carbonate Precipitation(MICP)is an emerging,environmental-friendly,and sustainable technology that has shown great potential for soil stabilization.However,its process efficiency has been re-cognized as a major challenge for its practical application in engineering.Non-fat powdered milk(NFPM)has been shown to have positive effects in enzymatical-induced carbonate precipitation(EICP),so in this study,we evaluated its use as an additive in the MICP process.A comparison between conventional MICP and NFPM-modified MICP was conducted,including chemical conversion efficiency,urea hydrolysis rate,and mechanical performance of sandy soils.A series of laboratory tests including precipitation analysis,unconfined compressive strength(UCS),and microstructure analysis were conducted.The results showed that the addition of NFPM could improve urease activity,enhance chemical conversion efficiency,and lead to superior strength im-provement compared to conventional MICP.Microstructure and particle size analysis revealed that the presence of NFPM was beneficial for larger crystal cluster formation between sand grains,which could result in stronger bonds and better mechanical performance.In summary,this study indicates that the use of NFPM in MICP process can represent a more sustainable and economically viable approach for soil stabilization.The findings provide valuable information for engineers and researchers working in soil stabilization and environmental engineering,highlighting the potential of using natural additives such as NFPM to promote the sustainable development of MICP technique.展开更多
The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine m...The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine milk exosomes.The milk exosome partition behaviors and bovine milk separation were investigated,and the ATPSs and bovine milk whey addition was optimized.The optimal separation conditions were identified as 16%(mass)polyethylene glycol 4000,10%(mass)dipotassium phosphate,and 1%(mass)enzymatic hydrolysis bovine milk whey.During the separation process,bovine milk exosomes were predominantly enriched in the interphase,while protein impurities were primarily found in the bottom phase.The process yielded bovine milk exosomes of 2.0×10^(11)particles per ml whey with high purity(staining rate>90%,7.01×10^(10)particles per mg protein)and high uniformity(polydispersity index<0.03).The isolated exosomes were characterized and identified by transmission electron microscopy,zeta potential and size distribution.The results demonstrated aqueous two-phase extraction possesses a robust capability for the enrichment and separation of exosomes directly from bovine milk whey,presenting a novel approach for the large-scale isolation of exosomes.展开更多
Background Dysregulation of lipid metabolism and its consequences on growth performance in young ruminants have attracted attention,especially in the context of alternative feeding strategies.This study aims to elucid...Background Dysregulation of lipid metabolism and its consequences on growth performance in young ruminants have attracted attention,especially in the context of alternative feeding strategies.This study aims to elucidate the effects of milk replacer(MR)feeding on growth,lipid metabolism,colonic epithelial gene expression,colonic microbiota composition and systemic metabolism in goat kids compared to breast milk(BM)feeding,addressing a critical knowledge gap in early life nutrition.Methods Ten female goat kids were divided into 2 groups:those fed breast milk(BM group)and those fed a milk replacer(MR group).Over a period of 28 d,body weight was monitored and blood and tissue samples were collected for biochemical,transcriptomic and metabolomic analyses.Profiling of the colonial microbiota was performed using 16S rRNA gene sequencing.Intestinal microbiota transplantation(IMT)experiments in gnotobiotic mice were per-formed to validate causality.Results MR-fed pups exhibited reduced daily body-weight gain due to impaired lipid metabolism as evidenced by lower serum and liver total cholesterol(TC)and non-esterified fatty acid(NEFA)concentrations.Transcriptomic analysis of the colonic epithelium revealed upregulated genes involved in negative regulation of lipid metabolism,concomitant with microbiota shifts characterized by a decrease in Firmicutes and an increase in Actinobacteria.Specifically,genera such as Bifidobacterium and Prevotella were enriched in the MR group,while Clostridium and Fae-calibacterium were depleted.Metabolomics analyses confirmed alterations in bile acid and fatty acid metabolic path-ways.IMT experiments in mice recapitulated the metabolic phenotype observed in MR-fed goats,confirming the role of the microbiota in modulating host lipid metabolism.Conclusions Milk replacer feeding in goat kids disrupts lipid metabolism and gut microbiota dynamics,result-ing in reduced growth rates and metabolic alterations.These findings highlight the importance of early nutritional intervention on metabolic programming and suggest that modulation of the gut microbiota may be a target for improving growth and metabolic health in ruminants.This study contributes to the understanding of nutritional management strategies in livestock and their impact on animal health and productivity.展开更多
Background As an essential source of nutrients for young mammals,milk possesses a variety of biological functions.Recently identified milk-derived small extracellular vesicles(sEV)have shown potential regulatory effec...Background As an essential source of nutrients for young mammals,milk possesses a variety of biological functions.Recently identified milk-derived small extracellular vesicles(sEV)have shown potential regulatory effects on intestinal health.Current studies have highlighted the functional roles of milk-derived sEV and their RNA cargo in promoting intestinal health.However,there is a paucity of research demonstrating how milk-derived sEV influence intestinal barrier function through the transport of circRNAs.Results In this study,we aimed to investigate the effects of porcine milk sEV(PM-sEV)circRNA on intestinal barrier function.We systematically identified the circRNAs involved in this process and analyzed the miRNAs through which PM-sEV deliver circRNAs to regulate intestinal barrier function.Our findings revealed that PM-sEV promote the expression of the intestinal tight junction proteins ZO-1 and Occludin,both in vivo(mice)and in vitro(IPEC-J2).When PMsEV RNA was reduced using ultrasound treatment,their ability to enhance intestinal barrier function was significantly reduced.Bioinformatics analysis showed that circ-0000197,present in PM-sEV,can target miR-429,while miR-429 has the ability to target the 3’-UTR of ZO-1 and Occludin.Furthermore,experiments involving the overexpression or inhibition of the relevant non-coding RNAs(ncRNAs)demonstrated that circ-0000197 significantly enhances intestinal barrier function,whereas miR-429 exerts an inhibitory effect on this function.Overall,our findings identify circ-0000197 in PM-sEV as a crucial circRNA that regulates intestinal barrier function by inhibiting miR-429.Circ-0000197 carried by PM-sEV acts as a competing endogenous RNA(ceRNA)that regulates the expression of ZO-1 and Occludin by sponging miR-429,thereby promoting intestinal barrier function at both the cellular and in vivo levels.Conclusions These findings emphasize the vital role of circRNAs transported through milk-derived sEV in regulating intestinal health,offering new avenues for developing innovative functional milk components.This mechanism also underscores the importance of PM-sEV carrying circ-0000197 in preserving intestinal barrier integrity.Collectively,this study enhances our understanding of the complex regulatory networks involving PM-sEV carrying circRNAs and their impact on intestinal health.展开更多
[Objectives]This study was conducted to investigate the influence mechanisms of microbial succession in raw milk under cold storage at different temperatures.[Methods]A raw milk sample was collected from a local large...[Objectives]This study was conducted to investigate the influence mechanisms of microbial succession in raw milk under cold storage at different temperatures.[Methods]A raw milk sample was collected from a local large-scale farm in Tangshan and divided into four treatment gradients:a control group(M)rapidly frozen at-80℃,and three experimental groups stored at 4℃(T),6℃(F),and 8℃(Y),respectively.A time series experiment was carried out according to time intervals of 24,48 and 72 h in each experimental group.Traditional microbial culture methods and 16S rRNA high-throughput sequencing were combined to analyze the dynamic changes in microbial abundance and structural variation.[Results]Plate counting revealed significantly lower total bacterial count and psychrotrophic bacteria in the 4℃storage group within 24 h compared with other treatment groups(P<0.01),confirming that maintaining low-temperature cold chain integrity and controlling treatment time(<24 h)can effectively inhibit microbial metabolic activity.16S rRNA sequencing analysis revealed high initial microbial diversity in raw milk,with dominant genera being Lactococcus,Acinetobacter,and Pseudomonas.Low-temperature treatment effectively reduced theαdiversity index of the microbial community.During the later stage of cold storage at 4℃,the relative abundance of Pseudomonas increased to over 90%,making it the dominant bacterial genus.[Conclusions]This study has significant application value for maintaining the quality of milk and dairy products and prolonging their shelf life.展开更多
The increased risk of chronic diseases has led to increasing importance of coarse foods in daily life,but the inclusion of new ingredients has a great degree of influence on the structural characteristics and sensory ...The increased risk of chronic diseases has led to increasing importance of coarse foods in daily life,but the inclusion of new ingredients has a great degree of influence on the structural characteristics and sensory qualities of the food.The effects of five different particle size variations on the physicochemical characteristics,dough,and steamed bread structure of Flaxseed-based milk coproduct(FMC)were investigated.As the particle size decreases,the structure of the dough becomes denser due to an increase in water retention capacity and dissolution capacity,weakening the competition for dough moisture and allowing for an increase in air-holding capacity.The reduction in particle size increased the specific volume of the steamed bread,a decrease in the spread ratio,and an optimization of hardness and elasticity,as well as an increase in consumer acceptance of the FMC steamed bread.However,it is not the smaller the particle size,the higher the quality of steamed bread,appropriate reduction of particle size can improve the quality of steamed bread.In addition,the addition of FMC reduces fat digestion.Therefore,the present study proposes a method to change the particle size of FMC to optimize the quality of the steamed bread and to reduce fat digestibility by adding FMC.展开更多
Autism spectrum disorder(ASD)is a neurodevelopmental disorder influenced by genes and the environment.This study investigated the protective effects of bovine milk fat globule membrane(BMFGM)and goat milk fat globule ...Autism spectrum disorder(ASD)is a neurodevelopmental disorder influenced by genes and the environment.This study investigated the protective effects of bovine milk fat globule membrane(BMFGM)and goat milk fat globule membrane(GMFGM)supplementation on ASD model mice.Analysis of phospholipid composition showed higher levels of phosphatidylcholine in BMFGM and phosphatidylethanolamine in GMFGM.Behavioral results indicated MFGMs ameliorated social deficits,with GMFGM being more effective.Milk fat globule membrane(MFGM)mitigated neuroinflammation by suppressing microglial overactivation and proinflammatory cytokines expression,meanwhile GMFGM increased the anti-inflammatory factor interleukin(IL)-10.MFGMs also altered gut microbiota composition and maintained gut barrier integrity.Uniquely,GMFGM increased butyrate production.Correlation analysis revealed positive associations between social behavior,levels of phosphatidylcholine,sphingomyelin,and the abundance of Allobaculum,Clostridium_sensu_stricto,and Turicibacter.Overall,these findings revealed the protective effects of MFGMs on neurodevelopment in ASD animal models and the underlying mechanism could be partly explained by their regulation of gut microbiota by the phospholipid components in MFGM.展开更多
Milk fat globules(MFG)are the primary source of energy and functional factor in dairy-based products.To evaluate the suitability of commercial MFG substitutes for infant and adult consumers,the lipolysis properties an...Milk fat globules(MFG)are the primary source of energy and functional factor in dairy-based products.To evaluate the suitability of commercial MFG substitutes for infant and adult consumers,the lipolysis properties and regulatory factors in digesting bovine,caprine,and human MFG were compared.The lipolysis kinetic models of bovine and caprine MFG are comparable,with bovine MFG displaying the highest degree of lipolysis(50.21%)during gastric digestion in infants,resulting in the production short-chain saturated fatty acids.Caprine MFG has a high rate of lipolysis due to its weak phospholipid-glycoprotein layer,which is prominent in both infants'and adults'digestion.The effects of consuming bovine and caprine MFG for adults are comparable in terms of lipolysis properties and nutritional value of fatty acid.After gastrointestinal digestion,human MFG has the highest digestibility(93.98%).The advantages of human MFG include its phospholipid structure,exogenous lipase,and lipolysis-regulating proteins.This study provides recommendation for the selection of milk fat substitutes and improvements in commercial dairy.展开更多
BACKGROUND Upper gastrointestinal hemorrhage is a life-threatening manifestation of cow’s milk protein allergy(CMPA).We analyze the clinical characteristics of a case of milk protein allergy manifested as severe uppe...BACKGROUND Upper gastrointestinal hemorrhage is a life-threatening manifestation of cow’s milk protein allergy(CMPA).We analyze the clinical characteristics of a case of milk protein allergy manifested as severe upper gastrointestinal hemorrhage.CASE SUMMARY The hospital admitted a 2-month-old male infant due to“melena for 6 days,he-matemesis twice”.The main symptom was melena,initially occurring once or twice per day,then gradually increasing to five or six times per day at their peak.During the course of the illness,the infant vomited blood,but there were no re-ports of vomiting,fever,pale complexion,dyspnea,wheezing,or difficulty brea-thing.Laboratory tests showed hemoglobin level of 87 g/L,platelet count of 349×109/L,and eosinophil percentage of 0.031.Coagulation studies were normal.After avoiding certain foods and feeding with an amino acid formula for 2 weeks,a repeat gastroscopy revealed less bleeding.After six weeks,a positive oral food challenge test confirmed a severe CMPA.At the 4-month follow-up,there was no gastrointestinal bleeding,and the infant was growing and developing well.CONCLUSION The manifestations of milk protein allergy are diverse and nonspecific,with gas-trointestinal bleeding being less common,especially in infants.When infants present with unexplained massive hematemesis,it’s critical to investigate the possibility of CMPA.展开更多
文摘BACKGROUND Human milk oligosaccharides(HMOs)are bioactive components of breast milk with diverse health benefits,including shaping the gut microbiota,modulating the immune system,and protecting against infections.HMOs exhibit dynamic secretion patterns during lactation,influenced by maternal genetics and environ-mental factors.Their direct and indirect antimicrobial properties have garnered significant research interest.However,a comprehensive understanding of the secretion dynamics of HMOs and their correlation with antimicrobial efficacy remains underexplored.AIM To synthesize current evidence on the secretion dynamics of HMOs during lactation and evaluate their antimicrobial roles against bacterial,viral,and protozoal pathogens.METHODS A systematic search of PubMed,Scopus,Web of Science,and Cochrane Library focused on studies investigating natural and synthetic HMOs,their secretion dynamics,and antimicrobial properties.Studies involving human,animal,and in vitro models were included.Data on HMO composition,temporal secretion patterns,and mechanisms of antimicrobial action were extracted.Quality assess-ment was performed using validated tools appropriate for study design.RESULTS A total of 44 studies were included,encompassing human,animal,and in vitro research.HMOs exhibited dynamic secretion patterns,with 2′-fucosyllactose(2′-FL)and lacto-N-tetraose peaking in early lactation and declining over time,while 3-fucosyllactose(3-FL)increased during later stages.HMOs demonstrated significant antimicrobial properties through pathogen adhesion inhibition,biofilm disruption,and enzymatic activity impairment.Synthetic HMOs,including bioengineered 2′-FL and 3-FL,were structurally and functionally comparable to natural HMOs,effectively inhibiting pathogens such as Pseudomonas aeruginosa,Escherichia coli,and Campylobacter jejuni.Additionally,HMOs exhibited synergistic effects with antibiotics,enhancing their efficacy against resistant pathogens.CONCLUSION HMOs are vital in antimicrobial defense,supporting infant health by targeting various pathogens.Both natural and synthetic HMOs hold significant potential for therapeutic applications,particularly in infant nutrition and as adjuncts to antibiotics.Further research,including clinical trials,is essential to address gaps in knowledge,validate findings,and explore the broader applicability of HMOs in improving maternal and neonatal health.
基金supported by grants from the National Natural Science Foundation of China(grant number:32072756)Agricultural Research System of China(grant number:CARS-36).
文摘Background Milk synthesis is an energy-intensive process influenced by oxygen availability.This study investigates how hypoxia affects milk synthesis in BMECs,focusing on key genes involved in lactation and energy metabolism.Methods BMECs were cultured in a normoxic environment and then transferred to a hypoxia chamber with 1%O2 for specified durations.The study evaluated cellular responses through various molecular experiments and RNA sequencing.Small interfering RNA was employed to knock down HIF-1αto investigate whether the lactation-related phenotype alteration depends on HIF-1α.Results Hypoxia disrupted milk protein production by reducing mTOR/P70S6K/4EBP1 signaling and downregulating genes critical for amino acid transport and protein synthesis.Triglyceride synthesis increased due to enhanced fatty acid uptake and the upregulation of regulatory proteins,including FASN and PPARγ.Although glucose uptake was elevated under hypoxia,key enzymes for lactose synthesis were downregulated,suggesting a redirection of glucose toward energy production.Mitochondrial function was impaired under hypoxia,with reduced gene expression in TCA cycle,ETC,cytosol-mitochondrial transport,decreased ATP levels,increased ROS levels,and structural alterations.Additionally,lipid synthesis and glucose uptake depend on HIF-1α,while milk protein synthesis alterations occurred independently of HIF-1α.Conclusions Hypoxia alters milk synthesis in BMECs by disrupting milk protein synthesis,enhancing lipid metabolism,and impairing energy production.These findings provide valuable insights into the molecular mechanisms underlying the effect of oxygen deprivation on lactation efficiency,offering potential targets for mitigating hypoxic stress in the mammary glands of dairy animals.
文摘BACKGROUND Although breast milk is ideal for newborns,in some cases,it is replaced with cow’s milk,which contains proteins that increase the risk of cow’s milk protein allergy(CMPA).AIM To evaluate CMPA prevalence in Bahrain and compare clinical characteristics of children with immunoglobulin E(IgE)-and non-IgE-mediated CMPA.METHODS This retrospective cohort study examined children with CMPA diagnosed at the pediatric gastroenterology outpatient clinic of the Salmaniya Medical Complex,Bahrain,between 2014 and 2022,and assessed CMPA prevalence.Clinical presentations,laboratory findings,dietary modifications,and outcomes were compared between children with IgE-mediated and non-IgE-mediated CMPA.Categorical variables were compared using Fisher’s exact test or Pearson’sχ2 test,whereas continuous variables were compared using Student’s t-test or the Mann-Whitney U test.RESULTS Of 8332 patients,6022(72.3%)adhered to their appointment.Of them,618(10.3%)were suggested of having CMPA and 595(96.3%)were included;CMPA prevalence was 2%.Most patients were Bahraini(93.8%)and males(55.3%).Non-IgE-mediated type accounted for 77.3%cases and IgE-mediated type,22.7%.IgE-mediated CMPA presented more in boys(P=0.030),and later in life(5.1 months±4.5 months vs 4.2 months±4.2 months,P=0.016,95%CI:0.08-1.73),had more associated diseases(P<0.001);and presented with more cutaneous(P=0.024)and respiratory(P=0.003)manifestations,severe symptoms[rash/dry skin(P=0.031),facial swelling/angioedema(P=0.003),failure to thrive(P=0.013),apparent life-threatening event(P<0.001)],and positive physical findings(P=0.002)than non-IgE-mediated CMPA.Most patients were exclusively fed cow milk formula(50.3%).The amino acid-based formula(AAF)was most prescribed(60.5%)with no difference between the two types(P=0.173).Although breastfeeding was recommended to 49.6%,only 8.2%were exclusively breastfed.IgE-mediated CMPA was associated with a longer follow-up duration than non-IgE-mediated CMPA(17.3 months±14.0 months vs 13.5 months±13.4 months,P=0.005,95%CI:1.1-6.3).CONCLUSION This study revealed a high CMPA prevalence with clinical differences between both types that can influence treatment.AAF was most prescribed,while breastfeeding with dietary modification is rarely applied.
文摘Breast milk represents the gold standard for neonatal nutrition,especially for preterm and term infants with a low birthweight.This awareness is based not only on the nutritional properties of human milk,which is specifically designed for the growth of humans but also on breast milk’s non-nutritional properties,such as protection against infection.In fact,breast milk should be considered a heterogeneous ecosystem,including a wide range of cells in addition to those involved in immune function;growth factors,such as vascular endothelial growth factor;multiple noncoding microRNAs;immune cells;epithelial cells and multipotent mesenchymal stem cells.This recent identification of a pool of progenitor stem cells in human milk is the driving force behind the growing research aimed at identifying the nature of these stem/progenitor cells and their sources.
基金supported by funding from Texas A&M AgriLife ResearchPrairie View A&M University International Center for Goat Research。
文摘Background Lactational performance depends heavily on age,health,and nutrition.L-Citrulline(Cit)is an effective precursor of L-arginine(Arg),an amino acid that has important roles in synthesis of nitric oxide(NO)and polyamines.Ruminal microbes degrade extracellular Arg;however,extracellular L-citrulline(Cit)is not degraded by ruminal microbes due to lack of uptake and can be fed unencapsulated as a precursor for Arg.As NO is a vasodilator,an increase in blood flow and transport of molecules to mammary tissue may enhance lactational performance and milk composition.Increases in polyamine production may increase milk protein synthesis within mammary tissue,thus increasing milk protein content.This study determined,for the first time,effects of dietary Cit supplementation on milk production and milk composition of Alpine dairy goats.Methods Does were synchronized to estrus and bred to Alpine bucks.Parturition was induced on d 149 of gestation and does were suckled overnight allowing kid(s)to obtain colostrum before being milked 24 h later(d 1 of lactation).Does were assigned to either control(CON,n=24)or Cit(CIT,n=23)diets.The isonitrogenous control diet consisted of 97.63%basal diet and 2.37%supplement(1.37%L-alanine and 1.00%soybean hydrogenated oil).The CIT supplemented diet consisted of 97.63%basal diet and 2.37%supplement(0.5%Cit,0.5%L-glutamine,1%soybean hydrogenated oil,0.37%cornstarch).Diets were group fed ad-libitum by treatment group.Blood samples were collected on d 0 and 30 of lactation,milk volumes measured twice daily,and on d 10,20,and 40 of lactation,milk samples were collected.Results CIT-treated does had greater daily milk production(P<0.05)and there was an effect of day of lactation on daily milk production(P<0.0001).Sire had significant effect on daily milk production as well(P<0.05).Milk compositional analyses revealed Cit supplementation increased solid-non-fat(SNF;P<0.05)and protein(P<0.05)content in milk.Conclusions Our novel results indicate that dietary supplementation of Cit fed ad-libitum in Alpine does increased daily milk yield,milk SNF content,and protein content.Supplemental Cit may be a proxy for Arg in goats to enhance lactational performance.
基金Supported by the Yakult Honsha Co.,Ltd.,No.1226/FTP-UGM/HK/2018.
文摘BACKGROUND Health maintenance in elderly houses includes management of the gut microbiota and the environment.Lacticaseibacillus paracasei Shirota(LcS)is a probiotic strain that positively affects the human gut.However,the evidence of its effects on the Indonesian population remains limited.AIM To investigate the effect of LcS-fermented milk on the gut microbiota and environment of Indonesian elderly houses.METHODS This double-blind,randomized,placebo-controlled trial involved 112 participants from Indonesian elderly houses,spanning a 2-week baseline and 24-week treatment.Participants were randomly assigned to probiotic or placebo groups,consuming fermented milk with or without LcS(>6.5×10^(9)colony-forming units).Fecal samples were collected every three months.Gut microbiota analysis was performed using 16S rRNA gene sequencing and reverse transcription quantitative polymerase chain reaction,while gut environment was assessed by measuring fecal organic acids,amino acid metabolites,and stool frequency.RESULTS Analyses of 16S rRNA gene sequence data at the 3-month period revealed increased Bifidobacterium and Succinivibrio and decreased Rikenellaceae RC9 gut group in the probiotic group.These shifts were associated with significant differences inβ-diversity metrics.The change in Bifidobacterium was confirmed by reverse transcription quantitative polymerase chain reaction,demonstrating higher abundance in the probiotic group than in the placebo group(8.5±1.1 vs 8.0±1.1,log10 bacterial cells/g;P=0.044).At 6-month period,the differences in Succinivibrio and Rikenellaceae RC9 gut group persisted.The probiotic group showed higher butyrate levels than the placebo group at the 6-month period(5.04±3.11 vs 3.95±2.89,μmol/g;P=0.048).The effect on amino acid metabolites and stool frequency was not significant.CONCLUSION Daily intake of LcS positively affects the gut microbiota and environment of people living in Indonesian elderly houses.
基金Universiti Putra Malaysia Inisiatif Putra Siswazah Grant,with a reference to UPM.RMC.800-2/1/2022/GPIPS/9740400Ministry of Higher Education,Malaysia(01-01-20-2323FR,with reference code:FRGS/1/2020/STG01/UPM/02/2)for the financial support。
文摘Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigestible lactose and allergenic proteins.Coconut milk has been around for a long time,but its application is limited due to a perceived lack of specific nutrients,high saturated fat levels,and low acceptability.Recent evidence indicates,however,that the saturated fat and other plant-based components found in coconut milk are good for metabolic outcomes and brain health.The conversion of coconut milk to yoghurt will further improve its functionality by boosting its existing nutritional qualities.In this article,the nutritional value of coconut milk,as well as its potential downsides,its application as yoghurt,and suggestions for enhancing its nutritional functionality will be examined.
基金funded by Earmarked Fund for China Agriculture Research System(CARS-14)the Central Public-interest Scientific Institution Basal Research Fund(11021716001100B)。
文摘Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced intestinal disorders,and compared the regulation patterns associated with gut microbiome and metabolome.The results showed plant-based milk alleviated the ceftriaxone caused cecum swelling,colonic tissue damage and intestinal microecological disorders.Meanwhile,administered plant-based milk decreased levels of pro-inflammatory cytokine(tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and oxidative stresses(malondialdehyde(MDA)and myeloperoxidase(MPO)in the colon,as well as increasing the levels of tight junction proteins(Occludin,Claudin-1,and ZO-1)in the colon.Moreover,administration of plant-based milk modulated the intestinal microbiota by promoting the relative levels of beneficial bacteria(Bifidobacterium),and inhibiting the harmful bacteria genus(Enterococcus).Furthermore,plant-based milk treatment significantly modulated glycerophospholipids metabolism(e.g.glycerophosphocholine)and arachidonic acid metabolism(e.g.prostaglandin G2 and arachidonic acid)in the serum.In conclusion,plant-based milk could alleviate antibiotic-related imbalance of barrier function damage,gut microbiota disorders and the reduction of metabolic disorders,which lays a foundation for exploring anti-inflammatory and intestinal micro-ecological approach to plant-based milk.
基金supported by the Huzhou Science and Technology Plan Project(No.2022GZ56)the Education Department of Zhejiang Province Scientific Research Project(No.Y202248484),China.
文摘Dynamic changes in the physiochemical,structural,and flavor characteristics of ginger-juice milk curd were explored by texture analysis,scanning electron microscopy,rheometry,electronic tongue,and gas chromatography-mass spectrometry(GC-MS).Protein electrophoresis showed that ginger juice could hydrolyzeαs-,β-,andκ-casein.Curd formation was initiated at 90 s,marked by significant changes in intensity detected via intrinsic fluorescence.The contents of soluble protein and calcium decreased rapidly during coagulation,while the caseinolytic activity,storage moduli,loss moduli,hardness,adhesiveness,and water-holding capacity increased,resulting in a denser gel structure with smaller pores and fewer cavitations as observed by scanning electron microscopy.Electronic tongue analysis indicated that milk could neutralize the astringency and saltiness of ginger juice,rendering the taste of ginger-juice milk curd more akin to that of milk.Approximately 70 volatile components were detected in ginger-juice milk curd.α-Zingiberene,α-curcumene,β-sesquiphellandrene,andβ-bisabolene were the predominant volatile flavor compounds,exhibiting an initial decrease in content followed by stability after 90 s.Decanoic acid,γ-elemene,and caryophyllene were identified as unique volatile compounds after mixing of milk and ginger juice.Understanding the dynamic changes in these characteristics during coagulation holds significant importance for the production of ginger-juice milk curd.
基金supported by the National Natural Science Foundation of China (41977020)the China Agriculture Research System of MOF and MARA (CARS22)。
文摘oil aggregates profoundly impact soil sustainability and crop productivity, and they are influenced by complexinteractions between minerals and organics. This study aimed to elucidate the alterations in mineralogy and soilorganic carbon(SOC) following long-term green manure incorporation and the effect on soil aggregates. Based on 5-and 36-year field experiments, surface soil samples(0–20 cm) were collected from Alfisol and Ferrisol soilssubjected to rice–rice–winter fallow(CK) and rice–rice–Chinese milk vetch(MV) treatments to investigate aggregatestability, mineralogy, SOC composition, and soil microstructural characteristics. The results showed that high clay-content Ferrisol exhibited greater aggregate stability than low clay-content Alfisol. The phyllosilicates in Alfisolprimarily comprised illite and vermiculite, whereas those in Ferrisol with high-content free-form Fe oxides(Fed) weredominated by kaolinite. Additionally, the clay fraction in Ferrisol contained more aromatic-C than the clay fraction inAlfisol. The 36-year MV incorporation significantly increased the Ferrisol macroaggregate stability(9.57–13.37%),and it also facilitated the transformation of vermiculite into kaolinite and significantly increased the clay, Fed, and aromatic-C contents in Ferrisol. Backscattered electron(BSE)-scanning electron microscopy/energy dispersive X-ray spectroscopy(SEM/EDS) revealed a compact aggregate structure in Ferrisol with co-localization of Feoxides and kaolinite. Moreover, the partial least path model(PLS-PM) revealed that clay content directly improvedmacroaggregate stability, and that kaolinite and Fed positively and directly affected clay or indirectly modulated clay formation by increasing the aromatic-C levels. Overall, long-term MV incorporation promotes clay aggregation by affecting mineral transformation to produce more kaolinite and Fe oxides and retain aromatic-C, and it ultimately improves aggregate stability.
文摘Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted among 60 primi postnatal mothers(30 in each group).Study was conducted at the Postnatal ward of IMS and SUM Hospital,Bhubaneswar,Odisha.Tools used were structured sociodemographic pro forma,structured breast milk adequacy questionnaire,and structured rating scale to assess the adequacy of breast milk production.The duration of the study is 1 year.All the postnatal mothers who were primigravida and undergone cesarean section were chosen as the sample through a convenience sampling technique.Then,the samples were assigned to the intervention group(receiving neck massage)and control group(receiving standard postnatal care).The data were collected and analyzed using both descriptive and inferential statistics by SPSS version 20.Results:The study resulted a significant difference between the pretest and posttest score of breast milk production after neck massage(P=0.000).The mean score of the posttest(2.90±0.36)is more than the mean score of the pretest(2.89±0.36)of the baby’s weight(P<0.001).Further,the number of urination and stool passed in 24 h was more in the intervention group than the control group(P<0.001).The Chi-square analysis showed statistical significance between breast milk production after neck massage with religion of the postnatal mothers(P<0.05).Conclusion:The neck massage is found effective in improving the breast milk production among primi c-section mothers with improve newborn parameters.
基金supported by NSFC Major International Joint Research Project POW3M(51920105013)The authors also gratefully acknowledge the financial support from the National Natural Science Foundation of China(Grant No.51978315)The corresponding author also acknowledges support from the Jiangsu Government Scholarship for Overseas Studies(UJS-2023-001).
文摘Microbial-Induced Carbonate Precipitation(MICP)is an emerging,environmental-friendly,and sustainable technology that has shown great potential for soil stabilization.However,its process efficiency has been re-cognized as a major challenge for its practical application in engineering.Non-fat powdered milk(NFPM)has been shown to have positive effects in enzymatical-induced carbonate precipitation(EICP),so in this study,we evaluated its use as an additive in the MICP process.A comparison between conventional MICP and NFPM-modified MICP was conducted,including chemical conversion efficiency,urea hydrolysis rate,and mechanical performance of sandy soils.A series of laboratory tests including precipitation analysis,unconfined compressive strength(UCS),and microstructure analysis were conducted.The results showed that the addition of NFPM could improve urease activity,enhance chemical conversion efficiency,and lead to superior strength im-provement compared to conventional MICP.Microstructure and particle size analysis revealed that the presence of NFPM was beneficial for larger crystal cluster formation between sand grains,which could result in stronger bonds and better mechanical performance.In summary,this study indicates that the use of NFPM in MICP process can represent a more sustainable and economically viable approach for soil stabilization.The findings provide valuable information for engineers and researchers working in soil stabilization and environmental engineering,highlighting the potential of using natural additives such as NFPM to promote the sustainable development of MICP technique.
基金supported by the National Natural Science Foundation of China(22378350).
文摘The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine milk exosomes.The milk exosome partition behaviors and bovine milk separation were investigated,and the ATPSs and bovine milk whey addition was optimized.The optimal separation conditions were identified as 16%(mass)polyethylene glycol 4000,10%(mass)dipotassium phosphate,and 1%(mass)enzymatic hydrolysis bovine milk whey.During the separation process,bovine milk exosomes were predominantly enriched in the interphase,while protein impurities were primarily found in the bottom phase.The process yielded bovine milk exosomes of 2.0×10^(11)particles per ml whey with high purity(staining rate>90%,7.01×10^(10)particles per mg protein)and high uniformity(polydispersity index<0.03).The isolated exosomes were characterized and identified by transmission electron microscopy,zeta potential and size distribution.The results demonstrated aqueous two-phase extraction possesses a robust capability for the enrichment and separation of exosomes directly from bovine milk whey,presenting a novel approach for the large-scale isolation of exosomes.
基金financially supported by National Natural Science Foundation of China (32160801)China Agriculture Research System (CARS-39-12)+1 种基金Young Talent Fund of Association for Science and Technology in Shaanxi, China (2023-6-2-1)“Double-chain” project on livestock breeding (2022GDTSLD-46)
文摘Background Dysregulation of lipid metabolism and its consequences on growth performance in young ruminants have attracted attention,especially in the context of alternative feeding strategies.This study aims to elucidate the effects of milk replacer(MR)feeding on growth,lipid metabolism,colonic epithelial gene expression,colonic microbiota composition and systemic metabolism in goat kids compared to breast milk(BM)feeding,addressing a critical knowledge gap in early life nutrition.Methods Ten female goat kids were divided into 2 groups:those fed breast milk(BM group)and those fed a milk replacer(MR group).Over a period of 28 d,body weight was monitored and blood and tissue samples were collected for biochemical,transcriptomic and metabolomic analyses.Profiling of the colonial microbiota was performed using 16S rRNA gene sequencing.Intestinal microbiota transplantation(IMT)experiments in gnotobiotic mice were per-formed to validate causality.Results MR-fed pups exhibited reduced daily body-weight gain due to impaired lipid metabolism as evidenced by lower serum and liver total cholesterol(TC)and non-esterified fatty acid(NEFA)concentrations.Transcriptomic analysis of the colonic epithelium revealed upregulated genes involved in negative regulation of lipid metabolism,concomitant with microbiota shifts characterized by a decrease in Firmicutes and an increase in Actinobacteria.Specifically,genera such as Bifidobacterium and Prevotella were enriched in the MR group,while Clostridium and Fae-calibacterium were depleted.Metabolomics analyses confirmed alterations in bile acid and fatty acid metabolic path-ways.IMT experiments in mice recapitulated the metabolic phenotype observed in MR-fed goats,confirming the role of the microbiota in modulating host lipid metabolism.Conclusions Milk replacer feeding in goat kids disrupts lipid metabolism and gut microbiota dynamics,result-ing in reduced growth rates and metabolic alterations.These findings highlight the importance of early nutritional intervention on metabolic programming and suggest that modulation of the gut microbiota may be a target for improving growth and metabolic health in ruminants.This study contributes to the understanding of nutritional management strategies in livestock and their impact on animal health and productivity.
基金supported by the National key research and development program(2022YFD1300401)Natural Science Foundation of Guangdong Province(2023 A1515012127,2024 A1515010510,2025 A1515011832)+1 种基金National Natural Science Foundation of China(32372958)Visiting Scholar Fund by China Scholarship Council(202008440191).
文摘Background As an essential source of nutrients for young mammals,milk possesses a variety of biological functions.Recently identified milk-derived small extracellular vesicles(sEV)have shown potential regulatory effects on intestinal health.Current studies have highlighted the functional roles of milk-derived sEV and their RNA cargo in promoting intestinal health.However,there is a paucity of research demonstrating how milk-derived sEV influence intestinal barrier function through the transport of circRNAs.Results In this study,we aimed to investigate the effects of porcine milk sEV(PM-sEV)circRNA on intestinal barrier function.We systematically identified the circRNAs involved in this process and analyzed the miRNAs through which PM-sEV deliver circRNAs to regulate intestinal barrier function.Our findings revealed that PM-sEV promote the expression of the intestinal tight junction proteins ZO-1 and Occludin,both in vivo(mice)and in vitro(IPEC-J2).When PMsEV RNA was reduced using ultrasound treatment,their ability to enhance intestinal barrier function was significantly reduced.Bioinformatics analysis showed that circ-0000197,present in PM-sEV,can target miR-429,while miR-429 has the ability to target the 3’-UTR of ZO-1 and Occludin.Furthermore,experiments involving the overexpression or inhibition of the relevant non-coding RNAs(ncRNAs)demonstrated that circ-0000197 significantly enhances intestinal barrier function,whereas miR-429 exerts an inhibitory effect on this function.Overall,our findings identify circ-0000197 in PM-sEV as a crucial circRNA that regulates intestinal barrier function by inhibiting miR-429.Circ-0000197 carried by PM-sEV acts as a competing endogenous RNA(ceRNA)that regulates the expression of ZO-1 and Occludin by sponging miR-429,thereby promoting intestinal barrier function at both the cellular and in vivo levels.Conclusions These findings emphasize the vital role of circRNAs transported through milk-derived sEV in regulating intestinal health,offering new avenues for developing innovative functional milk components.This mechanism also underscores the importance of PM-sEV carrying circ-0000197 in preserving intestinal barrier integrity.Collectively,this study enhances our understanding of the complex regulatory networks involving PM-sEV carrying circRNAs and their impact on intestinal health.
基金Supported by Tangshan Talent Funding Project(A202202005)Dairy Industry Revitalization Major Technological Innovation Project of Hebei Key Research and Development Program(19227516D)High-level Talents Funding Project of Hebei Province(A201803034).
文摘[Objectives]This study was conducted to investigate the influence mechanisms of microbial succession in raw milk under cold storage at different temperatures.[Methods]A raw milk sample was collected from a local large-scale farm in Tangshan and divided into four treatment gradients:a control group(M)rapidly frozen at-80℃,and three experimental groups stored at 4℃(T),6℃(F),and 8℃(Y),respectively.A time series experiment was carried out according to time intervals of 24,48 and 72 h in each experimental group.Traditional microbial culture methods and 16S rRNA high-throughput sequencing were combined to analyze the dynamic changes in microbial abundance and structural variation.[Results]Plate counting revealed significantly lower total bacterial count and psychrotrophic bacteria in the 4℃storage group within 24 h compared with other treatment groups(P<0.01),confirming that maintaining low-temperature cold chain integrity and controlling treatment time(<24 h)can effectively inhibit microbial metabolic activity.16S rRNA sequencing analysis revealed high initial microbial diversity in raw milk,with dominant genera being Lactococcus,Acinetobacter,and Pseudomonas.Low-temperature treatment effectively reduced theαdiversity index of the microbial community.During the later stage of cold storage at 4℃,the relative abundance of Pseudomonas increased to over 90%,making it the dominant bacterial genus.[Conclusions]This study has significant application value for maintaining the quality of milk and dairy products and prolonging their shelf life.
基金support of the National Key Research and Development Program of China(2023YFD2100403)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI)+3 种基金the Earmarked Fund for CARS-14the Innovation Group Project of Hubei Province(2023AFA042)the Key Research Projects of Hubei Province(2020BBA045)the Knowledge Innovation Program of Wuhan-Basic Research(3562).
文摘The increased risk of chronic diseases has led to increasing importance of coarse foods in daily life,but the inclusion of new ingredients has a great degree of influence on the structural characteristics and sensory qualities of the food.The effects of five different particle size variations on the physicochemical characteristics,dough,and steamed bread structure of Flaxseed-based milk coproduct(FMC)were investigated.As the particle size decreases,the structure of the dough becomes denser due to an increase in water retention capacity and dissolution capacity,weakening the competition for dough moisture and allowing for an increase in air-holding capacity.The reduction in particle size increased the specific volume of the steamed bread,a decrease in the spread ratio,and an optimization of hardness and elasticity,as well as an increase in consumer acceptance of the FMC steamed bread.However,it is not the smaller the particle size,the higher the quality of steamed bread,appropriate reduction of particle size can improve the quality of steamed bread.In addition,the addition of FMC reduces fat digestion.Therefore,the present study proposes a method to change the particle size of FMC to optimize the quality of the steamed bread and to reduce fat digestibility by adding FMC.
基金supported by the Central Government Guidance Local Science and Technology Development Fund Project(2021Szvup119)the Key Research and Development Plan of Shaanxi Province(2023-YBNY-184)+3 种基金the Regional Consolidated Fund-Youth Fund Project in Guangdong Province(2022A1515110717)China Postdoctoral Science Foundation(2022M72261)Guangdong Basic and Applied Basic Research Foundation(2021A1515110813)the 2021-National Center of Technology Innovation for Dairy-5.
文摘Autism spectrum disorder(ASD)is a neurodevelopmental disorder influenced by genes and the environment.This study investigated the protective effects of bovine milk fat globule membrane(BMFGM)and goat milk fat globule membrane(GMFGM)supplementation on ASD model mice.Analysis of phospholipid composition showed higher levels of phosphatidylcholine in BMFGM and phosphatidylethanolamine in GMFGM.Behavioral results indicated MFGMs ameliorated social deficits,with GMFGM being more effective.Milk fat globule membrane(MFGM)mitigated neuroinflammation by suppressing microglial overactivation and proinflammatory cytokines expression,meanwhile GMFGM increased the anti-inflammatory factor interleukin(IL)-10.MFGMs also altered gut microbiota composition and maintained gut barrier integrity.Uniquely,GMFGM increased butyrate production.Correlation analysis revealed positive associations between social behavior,levels of phosphatidylcholine,sphingomyelin,and the abundance of Allobaculum,Clostridium_sensu_stricto,and Turicibacter.Overall,these findings revealed the protective effects of MFGMs on neurodevelopment in ASD animal models and the underlying mechanism could be partly explained by their regulation of gut microbiota by the phospholipid components in MFGM.
基金supported by the Young Scientists Fund in National Natural Science Foundation of China(31901650)Ministry of Science and Technology of the People’s Republic of China(2016YFD0401404)Innovation Fund of Tianjin University of Science and Technology for Young Teachers(2017LG01)。
文摘Milk fat globules(MFG)are the primary source of energy and functional factor in dairy-based products.To evaluate the suitability of commercial MFG substitutes for infant and adult consumers,the lipolysis properties and regulatory factors in digesting bovine,caprine,and human MFG were compared.The lipolysis kinetic models of bovine and caprine MFG are comparable,with bovine MFG displaying the highest degree of lipolysis(50.21%)during gastric digestion in infants,resulting in the production short-chain saturated fatty acids.Caprine MFG has a high rate of lipolysis due to its weak phospholipid-glycoprotein layer,which is prominent in both infants'and adults'digestion.The effects of consuming bovine and caprine MFG for adults are comparable in terms of lipolysis properties and nutritional value of fatty acid.After gastrointestinal digestion,human MFG has the highest digestibility(93.98%).The advantages of human MFG include its phospholipid structure,exogenous lipase,and lipolysis-regulating proteins.This study provides recommendation for the selection of milk fat substitutes and improvements in commercial dairy.
基金Supported by the Excellent Medical Talents Training Program of the First Affiliated Hospital of Guangxi Medical University,Difficult and Critical illness Center,Pediatric Clinical Medical Research Center of Guangxi,No.Gui Ke AD22035219the Key Laboratory of Children’s Disease Research in Guangxi’s Colleges and Universities,Education Department of Guangxi Zhuang Autonomous Region.
文摘BACKGROUND Upper gastrointestinal hemorrhage is a life-threatening manifestation of cow’s milk protein allergy(CMPA).We analyze the clinical characteristics of a case of milk protein allergy manifested as severe upper gastrointestinal hemorrhage.CASE SUMMARY The hospital admitted a 2-month-old male infant due to“melena for 6 days,he-matemesis twice”.The main symptom was melena,initially occurring once or twice per day,then gradually increasing to five or six times per day at their peak.During the course of the illness,the infant vomited blood,but there were no re-ports of vomiting,fever,pale complexion,dyspnea,wheezing,or difficulty brea-thing.Laboratory tests showed hemoglobin level of 87 g/L,platelet count of 349×109/L,and eosinophil percentage of 0.031.Coagulation studies were normal.After avoiding certain foods and feeding with an amino acid formula for 2 weeks,a repeat gastroscopy revealed less bleeding.After six weeks,a positive oral food challenge test confirmed a severe CMPA.At the 4-month follow-up,there was no gastrointestinal bleeding,and the infant was growing and developing well.CONCLUSION The manifestations of milk protein allergy are diverse and nonspecific,with gas-trointestinal bleeding being less common,especially in infants.When infants present with unexplained massive hematemesis,it’s critical to investigate the possibility of CMPA.
