[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Ki...[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.展开更多
Two new isosteroid alkaloids, named pengbeimine A and pengbeimine C, were isolated from the fresh bulbs of FritiUaria monatha Migo. Their structures were determined by spectral methods, and were proved by X-ray diffra...Two new isosteroid alkaloids, named pengbeimine A and pengbeimine C, were isolated from the fresh bulbs of FritiUaria monatha Migo. Their structures were determined by spectral methods, and were proved by X-ray diffraction as well.展开更多
[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substa...[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.展开更多
[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The contr...[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.展开更多
The effects of light on the quality and related genes of Dendrobium officinale were summarized, and the main chemical components and pharmacological effects of D. officinale were analyzed. The biomass accumulation of ...The effects of light on the quality and related genes of Dendrobium officinale were summarized, and the main chemical components and pharmacological effects of D. officinale were analyzed. The biomass accumulation of D. officinale is related to light quality and light intensity. Monochromatic light quality can make polysaccharide accumulate, and mixed light quality can promote cell proliferation and component accumulation of D. officinale. Under different light intensity, the polysaccharide content of D. officinale is different, and it changes with the change of light intensity. When a certain intensity is exceeded, the polysaccharide content decreases with the increase of light intensity. The chemical components of D. officinale are polysaccharides, alkaloids, amino acids, volatile components, etc. The analysis of the growth and components of D. officinale provides reference and basis for improving the quality and chemical components of D. officinale and promotes the research and development of related products of D. officinale.展开更多
The Yunnan-Guangxi-Guizhou rocky desertified area in southwestern China is one of the contiguous extremely poor areas identified in the Outline of Poverty Alleviation and Development in the Rural Areas of China(2011-2...The Yunnan-Guangxi-Guizhou rocky desertified area in southwestern China is one of the contiguous extremely poor areas identified in the Outline of Poverty Alleviation and Development in the Rural Areas of China(2011-2020).In rocky desertified areas,due to long-term severe soil erosion,large areas of bedrock are exposed or gravels are accumulated.This bare rock and gravel-mulched land has become the main land type in the rocky mountains.Under normal circumstances,it cannot be directly used for agriculture,forestry,animal husbandry,and fishery production,and is classified as land that is difficult to be utilized.In recent years,in Debao County,Baise City,Guangxi Zhuang Autonomous Region,as a deeply impoverished county in the Yunnan-Guangxi-Guizhou rocky desertified area,a new land use model of karst rocky desertified land,that is,planting Dendrobium officinale Kimura et Migo on bare rocky ground,has emerged,and certain poverty alleviation benefits have been achieved initially.In this article,on the basis of analyzing the suitability of planting D.officinale Kimura et Migo on karst rocky desertified land,the practice of planning D.officinale Kimura et Migo on the rocky desertified land in Debao County was elucidated,and then suggestions for reasonable promotion of ecological planting of D.officinale Kimura et Migo in karst rocky desertified areas were put forward.展开更多
[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skelet...[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skeletal muscle was established by treadmill training.Forty two SD rats were randomly divided into a control group,1,12 and 24 h exercise groups,D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group,and D.officinale 2 ml+24 h exercise group,with 6 rats in each group.Various D.officinale groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time,a week in advance.Except for the quiet group,the samples were collected from the 1,12 and 24 h exercise groups after anesthesia following 1,12 and 24 h of exercise for the last time,respectively,and the D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group and D.officinale 2 ml+24 h exercise group were also sampled after anesthesia following 1,12 and 24 h of exercise for the last time,respectively.The contents of ATP,CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay(ELISA).The histopathological changes of gastrocnemius muscle were observed by HE staining.PCR and Western-blot detection were carried out to analyze the effects of D.officinale on IGF-1 mRNA and protein expression in gastrocnemius muscle.[Results]Compared with the quiet group,the ATP contents in the serum of rats in the 1,12 and 24 h exercise groups significantly decreased(P<0.01),while the CK and CK-MM contents significantly increased(P<0.01).The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased(P<0.01).Compared with the 1 h exercise group,the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+1 h exercise group significantly increased(P<0.05),while the CK and CK-MM contents significantly decreased(P<0.01).Compared with the 12 h exercise group,the D.officinale liquid+12 h exercise group showed a significant increase in ATP content(P<0.01),significant increases in IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue(P<0.01),and significant decreases in CK and CK-MM contents(P<0.01).Compared with the 24 h exercise group,the ATP content and IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+24 h exercise group significantly increased(P<0.01),while the CK and CK-MM contents significantly decreased(P<0.01).From the pathological tissue morphology of the gastrocnemius muscle in rats with EIMD treated with D.officinale,it could be concluded that the gastrocnemius muscle of each exercise group was significantly damaged,and the damage was significantly alleviated after administration of D.officinale liquid.[Conclusions]The effects and mechanism of D.officinale on prevention and treatment of EIMD in rats might be related to the promotion of IGF-1 mRNA and protein expression in injured tissues by reducing ATP energy consumption,CK-MM and CK activity.展开更多
[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divi...[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divided into group A,group B and control group by randomized control method. Group A adopted D. officinale Kimura et Migo and metformin hydrochloride; group B adopted D. officinale Kimura et Migo; the control group adopted metformin hydrochloride. The fasting plasma glucose( FPG) of 3 groups was measured before the treatment and 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after the treatment,Hb A1 c was measured and diabetes symptom grade score was counted before the treatment and 12 weeks after treatment,observation indicators and clinical effect of 3 groups of patients were compared. [Results] The fasting plasma glucose level in group A was lower than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P >0. 05); Hb A1 c of group A for 12 weeks after treatment was lower than that of other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05); the difference in diabetes symptom grade score of group A was greater than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05). [Conclusions]D. officinale Kimura et Migo from Longhushan can effectively reduce the fasting plasma glucose level and Hb A1 c of type 2 diabetic patients and effectively improve the symptoms of patients; clinical application of D. officinale Kimura et Migo has equivalent effect with metformin hydrochloride,and combination of D. officinale Kimura et Migo with metformin hydrochloride has better effect than single use.展开更多
基金Supported by Plan Project of Science and Technology Committee of Guangxi Province (0322024-3A) Scientific Research Foundation for Returned Scholars of Guangxi Province~~
文摘[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
基金financially supported by the National Natural Science Foundation of China(No.30160097).
文摘Two new isosteroid alkaloids, named pengbeimine A and pengbeimine C, were isolated from the fresh bulbs of FritiUaria monatha Migo. Their structures were determined by spectral methods, and were proved by X-ray diffraction as well.
基金Supported by Project of National Natural Science Foundation(81460587)Program of Collaborative Innovation Center for Zhuang Medicine of Guangxi(Gui Jiao Ke Yan[2013]20)+2 种基金Key Laboratory Project of Zhuang Medicine of Guangxi(Gui Ke Ji Zi[2014]32)Key Discipline Zhuang Medicine Program of Guangxi(Gui Jiao Ke Yan[2013]16)Eight Scholar Program of Guangxi"TCM Innovation Theory and Efficacy Research"
文摘[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.
文摘[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.
基金Supported by National Natural Science Foundation of China (82060695)Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine (GKJZ[2014]32)+5 种基金Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine (ZYYDC-02)Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine (GJKY[2013]20)Guangxi Key Discipline:Zhuang Pharmacology (GJKY[2013]16)First-class Discipline in Guangxi:Chinese Pharmacology (0501802815)Development of a New Zhuang Medicine Huotanmu Capsule for the Treatment of Hepatitis B (NKF[2018]11)The 8th Batch of Specially Appointed Experts Project in Guangxi (GRCTZ[2019]13)。
文摘The effects of light on the quality and related genes of Dendrobium officinale were summarized, and the main chemical components and pharmacological effects of D. officinale were analyzed. The biomass accumulation of D. officinale is related to light quality and light intensity. Monochromatic light quality can make polysaccharide accumulate, and mixed light quality can promote cell proliferation and component accumulation of D. officinale. Under different light intensity, the polysaccharide content of D. officinale is different, and it changes with the change of light intensity. When a certain intensity is exceeded, the polysaccharide content decreases with the increase of light intensity. The chemical components of D. officinale are polysaccharides, alkaloids, amino acids, volatile components, etc. The analysis of the growth and components of D. officinale provides reference and basis for improving the quality and chemical components of D. officinale and promotes the research and development of related products of D. officinale.
文摘The Yunnan-Guangxi-Guizhou rocky desertified area in southwestern China is one of the contiguous extremely poor areas identified in the Outline of Poverty Alleviation and Development in the Rural Areas of China(2011-2020).In rocky desertified areas,due to long-term severe soil erosion,large areas of bedrock are exposed or gravels are accumulated.This bare rock and gravel-mulched land has become the main land type in the rocky mountains.Under normal circumstances,it cannot be directly used for agriculture,forestry,animal husbandry,and fishery production,and is classified as land that is difficult to be utilized.In recent years,in Debao County,Baise City,Guangxi Zhuang Autonomous Region,as a deeply impoverished county in the Yunnan-Guangxi-Guizhou rocky desertified area,a new land use model of karst rocky desertified land,that is,planting Dendrobium officinale Kimura et Migo on bare rocky ground,has emerged,and certain poverty alleviation benefits have been achieved initially.In this article,on the basis of analyzing the suitability of planting D.officinale Kimura et Migo on karst rocky desertified land,the practice of planning D.officinale Kimura et Migo on the rocky desertified land in Debao County was elucidated,and then suggestions for reasonable promotion of ecological planting of D.officinale Kimura et Migo in karst rocky desertified areas were put forward.
基金Supported by Undergraduate Innovation and Entrepreneurship Training Program of Guizhou University of Traditional Chinese Medicine(GZYDCHZ[2019]42)National Key R&D Plan(2019YFC1712500)Guizhou Provincial Science and Technology Planning Project(QKHHBZ[2020]3003),QSKH[2019006].
文摘[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skeletal muscle was established by treadmill training.Forty two SD rats were randomly divided into a control group,1,12 and 24 h exercise groups,D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group,and D.officinale 2 ml+24 h exercise group,with 6 rats in each group.Various D.officinale groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time,a week in advance.Except for the quiet group,the samples were collected from the 1,12 and 24 h exercise groups after anesthesia following 1,12 and 24 h of exercise for the last time,respectively,and the D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group and D.officinale 2 ml+24 h exercise group were also sampled after anesthesia following 1,12 and 24 h of exercise for the last time,respectively.The contents of ATP,CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay(ELISA).The histopathological changes of gastrocnemius muscle were observed by HE staining.PCR and Western-blot detection were carried out to analyze the effects of D.officinale on IGF-1 mRNA and protein expression in gastrocnemius muscle.[Results]Compared with the quiet group,the ATP contents in the serum of rats in the 1,12 and 24 h exercise groups significantly decreased(P<0.01),while the CK and CK-MM contents significantly increased(P<0.01).The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased(P<0.01).Compared with the 1 h exercise group,the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+1 h exercise group significantly increased(P<0.05),while the CK and CK-MM contents significantly decreased(P<0.01).Compared with the 12 h exercise group,the D.officinale liquid+12 h exercise group showed a significant increase in ATP content(P<0.01),significant increases in IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue(P<0.01),and significant decreases in CK and CK-MM contents(P<0.01).Compared with the 24 h exercise group,the ATP content and IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+24 h exercise group significantly increased(P<0.01),while the CK and CK-MM contents significantly decreased(P<0.01).From the pathological tissue morphology of the gastrocnemius muscle in rats with EIMD treated with D.officinale,it could be concluded that the gastrocnemius muscle of each exercise group was significantly damaged,and the damage was significantly alleviated after administration of D.officinale liquid.[Conclusions]The effects and mechanism of D.officinale on prevention and treatment of EIMD in rats might be related to the promotion of IGF-1 mRNA and protein expression in injured tissues by reducing ATP energy consumption,CK-MM and CK activity.
文摘[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divided into group A,group B and control group by randomized control method. Group A adopted D. officinale Kimura et Migo and metformin hydrochloride; group B adopted D. officinale Kimura et Migo; the control group adopted metformin hydrochloride. The fasting plasma glucose( FPG) of 3 groups was measured before the treatment and 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after the treatment,Hb A1 c was measured and diabetes symptom grade score was counted before the treatment and 12 weeks after treatment,observation indicators and clinical effect of 3 groups of patients were compared. [Results] The fasting plasma glucose level in group A was lower than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P >0. 05); Hb A1 c of group A for 12 weeks after treatment was lower than that of other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05); the difference in diabetes symptom grade score of group A was greater than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05). [Conclusions]D. officinale Kimura et Migo from Longhushan can effectively reduce the fasting plasma glucose level and Hb A1 c of type 2 diabetic patients and effectively improve the symptoms of patients; clinical application of D. officinale Kimura et Migo has equivalent effect with metformin hydrochloride,and combination of D. officinale Kimura et Migo with metformin hydrochloride has better effect than single use.
