马来西亚国际家具展(MIFF)于3月3日在吉隆坡国际贸易展览中心(MITEC)举办了其备受瞩目的2025年度颁奖典礼,将活动打造为一场设计巧思与工艺的灵感盛宴。马来西亚种植与原产业部副部长YBhg Dato'Razali Bin Mohammad作为主礼嘉宾出...马来西亚国际家具展(MIFF)于3月3日在吉隆坡国际贸易展览中心(MITEC)举办了其备受瞩目的2025年度颁奖典礼,将活动打造为一场设计巧思与工艺的灵感盛宴。马来西亚种植与原产业部副部长YBhg Dato'Razali Bin Mohammad作为主礼嘉宾出席典礼,代表该部秘书长YBhg Dato’Yusran Shah,表彰了东南亚具有家具设计全球影响力的杰出人才。展开更多
Object: To identify transcript variants and expression patterns of porcine Mitf. Materials and methods: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequent...Object: To identify transcript variants and expression patterns of porcine Mitf. Materials and methods: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. Results: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS.Conclusion: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF.展开更多
基金supported by grants from the Major State Basic Research Development Program of China(973 Program)(#2011CBA01000)the National Basic Research Program of China(973 Program)(#2012CB967900)the National Natural Science Foundation of China(81400472)
文摘Object: To identify transcript variants and expression patterns of porcine Mitf. Materials and methods: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. Results: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS.Conclusion: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF.