The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of pote...The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of potentially harmful elements(PHEs)that threaten human health and destabilize microbial communities.Anthropogenic climate change,driven by greenhouse gas emissions,alters water availability and soil composition,further affecting ecosystem balance and microbial diversity.This review aggregates findings from studies covering the last two decades to assess how mining pollution and climate change impact microbial diversity,their adaptation mechanisms,and the associated health risks.It reveals that environmental stressors favour resistant microbial taxa while eliminating sensitive species,thereby reshaping microbial ecosystems.Microorganisms demonstrate genetic,biochemical,and physiological adaptations that enable them to survive in polluted or changing environments,often resulting in a higher prevalence of pathogenic and antibiotic-resistant strains.These variations in microbial dynamics contribute to health challenges such as respiratory infections,foodborne illnesses,and increased exposure in children and immune-compromised individuals.The review highlights the linkages between environmental degradation,microbial ecology,and human health,underscoring the need for integrative policies and interventions to mitigate long-term risks,support microbial ecosystem stability,promote sustainable health and agricultural outcomes.展开更多
This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from...This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.展开更多
Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon&...Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.展开更多
To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metageno...To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metagenomic analysis.An airlift inner-circulation partition bioreactor(AIPBR)was developed,featuring an inner cylinder and a flow guide tube to create distinct oxygen gradients,facilitating the study of microbial adaptation under varying salt conditions.The AIPBR was operated with synthetic wastewater containing ammonium concentrations of 1800±100 mg/L and salinity gradients ranging from 1 to 10 g/L,followed by a fixed salinity period at 6 g/L,with ammonium concentrations approximately 850 mg/L.High-throughput metagenomic analysis revealed shifts in functional genes and metabolic pathways in response to salinity stress.Anammox bacteria adapted by enriching genes involved in the synthesis of osmoprotective compounds and activating energy-producing pathways like the tricarboxylic acid cycle(TCA).These adaptations,along with modifications in membrane composition,were essential for sustaining system stability under elevated salinity.Under prolonged high salinity stress,anaerobic ammonium oxidizing(AnAOB)exhibited improved salt tolerance,maintaining a total nitrogen removal efficiency above 85%and stabilizing after an adaptation phase.The metagenomic data revealed a marked enrichment of genes associated with ion transport,stress response mechanisms,and DNA repair pathways.Changes in microbial community composition favored salt-tolerant species,supporting system stability.These findings highlight the applicability of the developed bioreactor for scaling up the PNA process to handle high-salinity wastewater,providing a promising avenue for sustainable nitrogen removal in challenging environments.展开更多
Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),sal...Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),saline-alkaline(SA),and their combined(DSA)stress influence these ecological components remain poorly understood.This study investigated these mechanisms along natural gradients in a semi-arid grassland of China by analyzing soil physical-chemical properties,microbial communities,and vegetation characteristics.The results showed that as the environmental stress shifted from the D group to the DSA group and then to the SA group,soil electrical conductivity significantly increased,while urease and phosphatase activities significantly decreased.Soil organic carbon,total nitrogen,total phosphorus,and microbial biomass carbon and nitrogen were lower in the D and SA groups than in the DSA group.Meanwhile,plant biomass showed an increasing trend along the treatment gradient,primarily driven by dominant species,while plant diversity did not exhibit significant differences.Further analysis identified the soil water content and salinity as the key determinants of soil microbial diversity and community complexity.Soil enzyme activities exhibited contrasting relationships with microbial composition,correlating positively with the richness of bacterial amplicon sequence variants(ASVs)but negatively with the richness of fungal ASVs.Notably,microbial biomass,which varied significantly across different groups,emerged as a key predictor of changes in EMF,with its critical role confirmed through structural equation modeling.These findings collectively elucidate the responses of ecological communities to synergistic soil hydro-saline stress in semi-arid ecosystems,while highlighting the critical role of microbial biomass in maintaining EMF.展开更多
Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen micro...Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.展开更多
Agricultural ecosystems play a pivotal role in global carbon(C)sequestration efforts.Microbial C use efficiency(CUE)serves as a comprehensive metric that reflects the balance between microbial contributions to the acc...Agricultural ecosystems play a pivotal role in global carbon(C)sequestration efforts.Microbial C use efficiency(CUE)serves as a comprehensive metric that reflects the balance between microbial contributions to the accumulation and decomposition of soil organic C.However,the overall distribution patterns and underlying drivers of microbial CUE at the national scale remain unclear.Herein,data from 209 paired samples from 55 studies were analyzed to assess the distribution patterns and influencing factors of microbial CUE based on enzyme stoichiometry(CUE_(ST))in agricultural ecosystems across China.Results revealed that farmlands exhibited the highest CUE_(ST)value(mean=0.51),exceeding those of grasslands(0.46)and forests(0.44).Contrasting patterns of CUE_(ST)regulation were observed across land-use types,with farmlands showing significant(P<0.001)positive relationships of CUE_(ST)with phosphorus vs.nitrogen(N/P)limitation index,while grasslands and forests demonstrated inverse(P<0.05)relationships of CUE_(ST)with C limitation index.Nutrient stoichiometry emerged as the dominant driver of CUE_(ST),with enzyme ratios and mean annual precipitation playing secondary roles.Moreover,land management practices,including fertilization,grazing,and tillage,as well as land-use transition,significantly influenced microbial CUE_(ST)by potentially altering nutrient availability and soil properties;notably,water addition in grasslands had particularly positive effects.These findings provide a critical foundation for harnessing microbial CUE in agriculture and may inform scalable strategies to enhance soil C sequestration and climate-smart land management.展开更多
Objective Evidence suggests that depleted gut microbialα-diversity is associated with hypertension;however,whether metabolic markers affect this relationship remains unknown.We aimed to determine the potential metabo...Objective Evidence suggests that depleted gut microbialα-diversity is associated with hypertension;however,whether metabolic markers affect this relationship remains unknown.We aimed to determine the potential metabolites mediating the associations ofα-diversity with blood pressure(BP)and BP variability(BPV).Methods Metagenomics and plasma targeted metabolomics were conducted on 523 Chinese participants from the MetaSalt study.The 24-hour,daytime,and nighttime BP and BPV were calculated based on ambulatory BP measurements.Linear mixed models were used to characterize the relationships betweenα-diversity(Shannon and Chao1 index)and BP indices.Mediation analyses were performed to assess the contribution of metabolites to the observed associations.The influence of key metabolites on hypertension was further evaluated in a prospective cohort of 2,169 participants.Results Gut microbial richness(Chao1)was negatively associated with 24-hour systolic BP,daytime systolic BP,daytime diastolic BP,24-hour systolic BPV,and nighttime systolic BPV(P<0.05).Moreover,26 metabolites were strongly associated with richness(Bonferroni P<0.05).Among them,four key metabolites(imidazole propionate,2-hydroxy-3-methylbutyric acid,homovanillic acid,and hydrocinnamic acid)mediated the associations between richness and BP indices(proportions of mediating effects:14.1%–67.4%).These key metabolites were also associated with hypertension in the prospective cohort.For example,each 1-standard deviation unit increase in hydrocinnamic acid significantly reduced the risk of prevalent(OR[95%CI]=0.90[0.82,0.99];P=0.03)and incident hypertension(HR[95%CI]=0.83[0.71,0.96];P=0.01).Conclusion Our results suggest that gut microbial richness correlates with lower BP and BPV,and that certain metabolites mediate these associations.These findings provide novel insights into the pathogenesis and prevention of hypertension.展开更多
Elevation patterns and assembly processes of soil microbial community structures are essential for understanding biogeo-chemical processes in mountain systems.Differences in soil properties caused by elevation gradien...Elevation patterns and assembly processes of soil microbial community structures are essential for understanding biogeo-chemical processes in mountain systems.Differences in soil properties caused by elevation gradients can regulate the spatial distribu-tion and network complexity of the community structure.To explore the variations in soil microbial community structures and their as-sembly mechanisms across different elevations of the Changbai Mountains,as well as their responses to environmental factors,we col-lected microbial samples along an elevational gradient(seven elevations containing four vegetation zones)on the western slope of the Changbai Mountains using the method of metagenomic sequencing.The results showed a significant difference(P<0.05)for the Chao1 index across different elevations,but no significant difference was observed for the Shannon and Simpson indices.With increasing elev-ation,the number of nodes and links in the microbial network gradually decreased.Acidobacteria were highly connected to many nodes.The microbial communities indicated a significant distance-decay relationship(P<0.001)and were affected more by stochastic pro-cesses along the elevation gradient.The results of the Structural Equation Model(SEM)showed that elevation had direct significant ef-fect on carbon(C,P<0.01),nitrogen(N,P<0.01),and phosphorus(P,P<0.05)and weak negative effect on their ecological stoi-chiometry.Elevation was one of the major variables contributing to microbial network topology.The contribution of C and N to micro-bial network complexity was higher than that of P.Our study provides valuable insights into the responses of soil microbial communit-ies to elevation variations.展开更多
[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential e...[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential ecological functions.[Methods]Amplification sequencing analysis of bacterial and fungal communities in the rhizosphere soil of a peach orchard in Qian'an County,Tangshan City,Hebei Province,was performed using Illumina MiSeq high-throughput sequencing technology.[Results]The indices of Sobs,Chao,ACE,and Shannon for soil bacteria in the rhizosphere soil of peach trees were all higher than those for fungi,indicating a more uniform and diverse bacterial community structure.At the phylum level,the bacteria with relatively high abundance included Pseudomonadota(28.29%),Acidobacteriota(18.10%),Bacillota(12.17%),and Actinomycetota(11.73%).In contrast,the fungi with relatively high abundance were Ascomycota(64.64%),Basidiomycota(14.22%),and Mortierellomycota(14.09%).At the genus level,the bacteria with relatively high abundance comprised Sphingomonas(5.00%),Priestia(3.38%),Nitrospira(2.05%),etc.The fungi with relatively high abundance included Fusarium(13.13%),Mortierella(12.86%),Tausonia(6.97%),Neocosmospora(4.77%),etc.[Conclusions]This study offers a foundational dataset and theoretical reference for the regulation of rhizosphere microecology and the management of soil health in peach orchards in Tangshan.展开更多
Microbial contamination and the resulting corrosion in aircraft fuel system pose a serious threat to flight safety.Revealing the corrosion behavior and mechanism of fuel-degrading microorganisms on tank materials is c...Microbial contamination and the resulting corrosion in aircraft fuel system pose a serious threat to flight safety.Revealing the corrosion behavior and mechanism of fuel-degrading microorganisms on tank materials is crucial for developing effective mitigation strategies.In this study,the corrosion mechanisms of two representative hydrocarbon-degrading bacteria,Alcanivorax dieselolei and Microbacterium oxydans,toward AA7075 aluminum alloy,were systematically investigated.A combination of biofilm characterization,electrochemical testing,and surface/corrosion product characterization was employed.Both strains markedly accelerated the corrosion of AA7075,as evidence by the progressive decrease in polarization resistance and the pronounced rightward shift of the potentiodynamic polarization curves.Moreover,the difference between the pitting potential(E_(pit))and the corrosion potential(E_(corr))(ΔE=E_(pit)‒E_(corr))decreased due to microbial activities,indicating a pronounced tendency toward accelerated pitting corrosion.Corrosion morphology analysis revealed that both microbes promoted localized pitting corrosion.Furthermore,analysis of aviation kerosene composition indicated that both bacteria accelerated the degradation of C8 and C9 alkanes.These findings highlight the multiple threats of microbial contamination,material degradation,and fuel quality deterioration in fuel systems and underscore the need for targeted protection strategies for marine aviation operations.展开更多
As the core of cathode materials,sensitive metals play important roles in the optimization of acetate production from carbon dioxide(CO_(2))in microbial electrochemical system(MES).In this work,iron(Fe),copper(Cu),and...As the core of cathode materials,sensitive metals play important roles in the optimization of acetate production from carbon dioxide(CO_(2))in microbial electrochemical system(MES).In this work,iron(Fe),copper(Cu),and nickel(Ni)as sensitive metal cathode materials were evaluated for CO_(2) conversion in MES.The MES with Feelectrode as a promising electrode material demonstrated a superior CO_(2) reduction performance with a maximum acetate accumulation of 417.9±39.2 mg/L,which was 1.5 and 1.7 folds higher than that in the Ni-electrode and Cu-electrode groups,respectively.Furthermore,an outstanding electron recovery efficiency of 67.7%was shown in the Fe-electrode group.The electron transfer between electrode-suspended sludge was systematically cross-evaluated by the electrochemical behavior and extracellular polymeric substances.The Fe-electrode group had the highest electron transfer rate with 0.194 s-1(k_(app)),which was 17.6 and 21.5 times higher than that of the Cu-and Ni-electrode groups,respectively.Fe-electrode was beneficial for reducing electrochemical impedance between the electrode and suspended sludge.Additionally,redox substances in extracellular polymeric substances of the Fe-electrode group were increased,implying more favorable electron transport dynamics.Simultaneously,enrichments of functional bacteria Acetoanerobium and increased key enzymes involved in the carbonyl pathway of the Fe-electrode group were observed,which also promoted CO_(2) conversion in MES.This study provides a perspective on evaluating the promising sensitive metal electrode material for the process of CO_(2) valorization in MES and offers a reference for the subsequent electrode modification.展开更多
Background An imbalance in the rumen microbiota caused by high-concentrate diets(HCD)is a significant endogenous trigger of mastitis.However,the underlying mechanisms remain largely unknown.Microbial extracellular ves...Background An imbalance in the rumen microbiota caused by high-concentrate diets(HCD)is a significant endogenous trigger of mastitis.However,the underlying mechanisms remain largely unknown.Microbial extracellular vesicles(mEVs)are critical mediators of microbe-host communication.However,the role of mEVs in rumen microbiota-mediated mastitis has not yet been reported.In this study,we used an HCD-induced rumen microbiota dysbiosis model to investigate the role of mEVs-derived from rumen microbiota in the pathogenesis of mastitis.Results Our results indicate that HCD leads to mastitis and systemic inflammation.Meanwhile,HCD-fed goats exhibited substantial rumen microbiota dysbiosis and the disruption of the rumen barrier.Transplanting rumen microbiota from HCD goats into mice induced both mastitis and systemic inflammation in the recipients.Specifically,HCD increases the production of mEVs carrying microbial DNA,which can translocate across the compromised rumen barrier to the mammary gland,triggering a mammary inflammatory response via activation of the cGAS-STING-NF-κB/NLRP3 pathway.Furthermore,treating mice with mEVs isolated from the rumen fluid of HCD goats directly induced mastitis,whereas depletion of microbial DNA attenuated mEVs-induced mastitis.Conclusion Our findings suggest that HCD induces rumen microbiota dysbiosis and impairs rumen barrier function.This dysfunction leads to an increase in microbial DNA-containing mEVs,which subsequently leak into the mammary gland.Once there,these mEVs activate the cGAS-STING-NF-κB/NLRP3 signaling pathway,ultimately inducing mastitis.This study provides a new perspective on the“rumen microbiota-mammary gland axis”and enhances the understanding of the pathogenesis of mastitis.展开更多
Liquid-solid phase transfer promotes the interaction of perfluoroalkyl acids(PFAAs)with the microbial system of river sediments,which may affect the environmental behavior of antibiotic resistance genes(ARGs)contained...Liquid-solid phase transfer promotes the interaction of perfluoroalkyl acids(PFAAs)with the microbial system of river sediments,which may affect the environmental behavior of antibiotic resistance genes(ARGs)contained in benthic environments.Sediments collected from the receiving water of the largest fluoropolymer production facility in China were analyzed to investigate the impact of PFAAs on microbial communities and ARG profiles.The main contributors to the PFAAs were perfluorooctanoic acid and perfluorobutanoic acid,whose proportions(86.9%-93.4%)in the downstream surface sediments affected by industrial effluents were significantly higher than in the corresponding upstream samples(53.3%).A reduction in microbial diversity and richness was observed in the presence of high concentrations of PFAAs at the downstream sites.144 ARG subtypes,including three high-risk subtypes(bacA,aac(6′)-I and aadA),were identified in sediment samples.The discharge of fluorochemical effluents also results in a reduction of ARG diversity at subtype level.PFAAs exert a pronounced influence on the profile of ARGs in sediment.PFAAs and water quality parameters(e.g.pH and total phosphorus)were key drivers of the microbial community composition in the sediment.The regulation of microbial communities by PFAAs may represent an important pathway by which these compounds affect ARG profiles.展开更多
In this work,ofloxacin(OFL),a kind of frequently detected antibiotic in groundwater,was selected to explore its impact(at ng/L-μg/L-level)on denitrification performance in an autotrophic denitrification system driven...In this work,ofloxacin(OFL),a kind of frequently detected antibiotic in groundwater,was selected to explore its impact(at ng/L-μg/L-level)on denitrification performance in an autotrophic denitrification system driven by pyrite/sulfur(FeS2/S0).Results showed that OFL restrained nitrate removal efficiency,and the inhibition degree was positively related to the concentration of OFL.After being exposed to increased OFL(200 ng/L-100μg/L)for 69 days,higher inhibition of electron transport activity(ETSA),enzyme activities of nitrate reductase(NAR),and nitrite reductase(NIR)were acquired.Meanwhile,the extracellular protein(PN)content of sludge samples was remarkably stimulated by OFL to resist the augmented toxicity.OFL contributed to increased microbial diversity and sulfur/sulfide oxidation functional genes in ng/L-level bioreactors,whereas led to a decline inμg/L level experiments.With OFL at concentrations of 200 ng/L and 100μg/L,the whole expression of 10 key denitrification functional genes was depressed,and the higher the OFL concentration,the lower the expression level.However,no significant proliferation of antibiotic resistance genes(ARGs)either in 200 ng/L-OFL or 100μg/L-OFL groups was observed.Two-factor correlation analysis results indicated that Thiobacillus,Anaerolineae,Anaerolineales,and Nitrospirae might be the main hosts of existing ARGs in this system.展开更多
Land-use change and associated landscape pattern modifications are key drivers of biodiversity dynamics.However,the influence of landscape heterogeneity on soil microbial diversity remains poorly understood.This study...Land-use change and associated landscape pattern modifications are key drivers of biodiversity dynamics.However,the influence of landscape heterogeneity on soil microbial diversity remains poorly understood.This study analyzed soil microbial communities(bacteria and fungi)and soil properties(organic carbon,pH,available potassium,nitrogen,and phosphorus)across 74 sites in Hainan,China,covering six land-use types(rubber,areca,banana,farmland,longan,and mango).We evaluated the effects of both landscape composition(habitat coverage and patch richness)and configuration heterogeneity(patch density and largest patch index)on soil microbial community structure and diversity.Results show that land-use types and landscape patterns weakly affect microbial composition but strongly shape diversity in a taxon-specific way.Bacterial Chao1 richness and fungal Shannon diversity varied significantly among land-use types,mainly due to soil available phosphorus.Rubber coverage positively correlated with bacterial and fungal diversity,while the largest patch index negatively affected only fungal diversity.Available phosphorus reduced this negative effect.This study demonstrates that land-use change and landscape heterogeneity differentially influence soil microbial composition and diversity,with available phosphorus emerging as a key determinant.Our findings highlight the need for careful landscape planning and soil nutrient management to preserve soil microbial diversity.展开更多
Heavy metal contamination of soil is one of the major challenges to sustainable agriculture.This contamination can be transmitted through the soil food chain and poses a serious threat to human health.In this study,we...Heavy metal contamination of soil is one of the major challenges to sustainable agriculture.This contamination can be transmitted through the soil food chain and poses a serious threat to human health.In this study,we found that freeze-thaw leaching(FTL)effectively complements the low removal rate of chemical leaching,and investigated the effects of different numbers of FTL on Cd contamination,soil chemical properties and microbial communities.The results showed that repeated FTL significantly reduced(P<0.05)the total Cd content in the top soil(19.02%-49.35%)and subsoil(0.41%-21.13%)and promoted the transformation of Cd to a more stable form,mainly through various removal mechanisms such as complexation,ion-exchange,and chemical precipitation.This finding was supported by reductions in several soil properties,including pH,available potassium(AK),and available phosphorus(AP).FTL treatment initially increased the bioavailability of Cd compared to chemical leaching,but bioavailability of Cd progressively decreased as the number of freeze-thaw cycles increased.Additionally,FTL reduced the richness and diversity of bacteria communities,destabilized ecological symbiotic networks,while increasing the richness and diversity of fungi in the soil.Various model analyses indicated that FTL treatment,available Cd,soil pH,AP and AK were the key drivers influencing the changes in microbial community structure.This study provides new insights and scientific bases for the effective management of heavy metal pollution in agricultural soils,the restoration of ecosystem health,and the improvement of soil sustainability.展开更多
Sedimentary microbial communities play an important role in driving biogeochemical cycles in river ecosystems.The Yellow River is one of the rivers with the highest turbidity over the world.However,limited is known ab...Sedimentary microbial communities play an important role in driving biogeochemical cycles in river ecosystems.The Yellow River is one of the rivers with the highest turbidity over the world.However,limited is known about the microbial variation and its influencing factors in the Yellow River.In this study,we examined the microbial communities and their influencing factors in the sediment of Upper and Mid–Lower reaches of the Yellow River.The results showed that Gammaproteobacteria were most dominant(with Hydrogenophilaceae being the predominant family)in the studied Yellow River sediments.Phyla of Deltaproteobacteria,Nitrospirae and family of Bacillaceae,Geobaceraceae were more abundant in the Mid–Lower reaches than in the Upper reaches,while phyla of Gammaproteobacteria,Verrucomicrobia and family of Caldilineaceae,Llumatobacteraceae were more abundant in the Upper reaches than in the Mid–Lower reaches.The microbial communities were predominantly affected by nutrient factors(such as NH_(4)^(+),TN and TP),followed by the spatial and the content of Chla in the Mid–Lower reaches,while they were by predominantly affected by spatial factors,followed by the nutrient factors and the content of Chla in the Upper reaches.The dominant microbial taxa were mostly correlated with COD,NH_(4)^(+),TP and temperature,but they responded differently to these physiochemical factors between the Upper and Mid–Lower reaches.In summary,the sedimentary microbial communities differ between the Upper and Mid–Lower reaches and respond differently to the environmental and spatial factors in the sediment of the Yellow River.展开更多
Microbially induced calcium carbonate precipitation(MICP)is an eco-friendly technology for soil improvement.Although numerous experiments have been conducted to solidify sand foundations using MICP,the mechanisms by w...Microbially induced calcium carbonate precipitation(MICP)is an eco-friendly technology for soil improvement.Although numerous experiments have been conducted to solidify sand foundations using MICP,the mechanisms by which grain interfacial morphologies influencethe MICP process remain unclear.This study utilized 3D-printed flowcells with different boundary morphologies to investigate the effects of interfacial morphologies on the MICP process.CaCO_(3)precipitation characteristics were investigated through microscopic observation and image quantificationanalysis.The results indicate that low flowvelocities near the interface promote bacterial accumulation due to reduced hydrodynamic shear forces.Rough interfaces,compared to smooth ones,enhance bacterial adsorption owing to the larger regions of low flowvelocity,increased surface area,and the formation of local eddies,which promote greater CaCO_(3)precipitation.Compared to the regions away from the interface,a higher abundance of small CaCO_(3)crystals is observed near the interface because of the high urease activity from bacteria and the reduced shear-induced entrainment due to the low flowvelocity.Besides,larger crystals also preferentially precipitate in proximity to interfaces as the low flowvelocity enhances crystal growth according to the particle attachment theory.The presence of rough interfaces further reduces flowvelocities,leading to the precipitation of larger and more densely packed CaCO_(3)crystals.Therefore,rough interfaces promote the microbially induced calcium carbonate precipitation.This work is expected to enhance the understanding of microbially induced calcium carbonate precipitation characteristics on solid surfaces such as soil grains and contribute to the optimization of MICP applications.展开更多
Microplastics (MPs), fluoride (FR), and fungicide mancozeb (MZ) are common contaminants in soil. However, there is no information about the combined impacts of MPs, FR, and MZ on tomato plant growth features in the li...Microplastics (MPs), fluoride (FR), and fungicide mancozeb (MZ) are common contaminants in soil. However, there is no information about the combined impacts of MPs, FR, and MZ on tomato plant growth features in the literature. This study aimed to investigate the effects of combined application of MPs, FR, and MZ (both analytical grade and commercial) on the growth and development of tomato plants and metagenomics of rhizosphere soil. A pot experiment was set up in an artificial greenhouse with two sets of treatments. One set was the combined application of MPs, FR, and analytical grade MZ (B2) and a control without the application of MPs, FR, and MZ (W2), and the other set was the combined application of MPs, FR, and commercial MZ (B3) and a control without the application of MPs, FR, and MZ (W3). No detrimental effects of MPs, FR, and MZ were detected on the growth parameters of tomato plants, including the number of leaves and chlorophyll concentration. However, tomato roots showed knot and nodulation-type structures, and metabolomic profiling revealed that combined exposure to MPs, FR, and MZ profoundly reprogrammed the primary metabolism in tomato roots, with marked alterations in carbohydrate and amino acid pathways. Metagenome whole genome sequencing showed that the B2 and B3 treatments profoundly affected soil microbial community composition, diversity, gene abundances, and functional gene variations compared to W2 and W3. Proteobacteria became the dominating phylum in B2 and B3, causing a significant shift in the microbiome. Its abundance soared to 66.7% in B2 and 75.4% in B3, compared to only 35.9% in W2 and 28.9% in W3. On the other hand, Actinobacteria decreased significantly from 55.6% in W2 and 63.8% in W3 to 18.1% in B2 and 9.6% in B3. This study highlights the microbial shifts due to combined application of MPs, FR, and MZ, providing evidence for understanding their environmental risks.展开更多
基金the Copperbelt University Africa Centre of Excellence for Sustainable Mining(CBUACESM)at Copperbelt University in Kitwe,Zambia for funding this work。
文摘The Earth's environment is undergoing significant transformation due to mining,pollution,and climate change.Although mining is essential for economic development,it contributes significantly to the release of potentially harmful elements(PHEs)that threaten human health and destabilize microbial communities.Anthropogenic climate change,driven by greenhouse gas emissions,alters water availability and soil composition,further affecting ecosystem balance and microbial diversity.This review aggregates findings from studies covering the last two decades to assess how mining pollution and climate change impact microbial diversity,their adaptation mechanisms,and the associated health risks.It reveals that environmental stressors favour resistant microbial taxa while eliminating sensitive species,thereby reshaping microbial ecosystems.Microorganisms demonstrate genetic,biochemical,and physiological adaptations that enable them to survive in polluted or changing environments,often resulting in a higher prevalence of pathogenic and antibiotic-resistant strains.These variations in microbial dynamics contribute to health challenges such as respiratory infections,foodborne illnesses,and increased exposure in children and immune-compromised individuals.The review highlights the linkages between environmental degradation,microbial ecology,and human health,underscoring the need for integrative policies and interventions to mitigate long-term risks,support microbial ecosystem stability,promote sustainable health and agricultural outcomes.
基金supported by the National Natural Science Foundation of China(Grant No.32160172)the Key Science-Technology Project of Inner Mongolia(2023KYPT0010)+1 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Grant No.2025QN03006)the 2023 Inner Mongolia Public Institution High-level Talent Introduction Scientific Research Support Project.
文摘This study establishes and validates a method for the precise quantification of aquatic microbial loads using microbial diversity absolute quantitative sequencing.By adding synthetic spike-in DNA to water samples from the Dahei River prior to DNA extraction and 16S rRNA gene sequencing,it generates standard curves to convert sequencing data into absolute microbial copy numbers.The method,which is proved highly accurate(R^(2)>0.99),reveals a clear contrast between the river sites:the upstream community has not only a significantly higher total microbial load but also a completely different makeup of species compared to the downstream site.This approach effectively overcomes the limitations of relative abundance analysis,providing a powerful tool for environmental monitoring,and proposes key steps for future standardization to ensure data comparability and integration.
基金supported by the Key Research and Development Program of Ningxia Hui Autonomous Region,China(2023BCF01029)the Shaanxi Provincial Science and Technology Project for Innovation Team,China(2023-CXTD-59)the Innovative Team Special Project of Northwest A&F University,China(XYTD2023-12)。
文摘Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.
基金supported by China Hunan Provincial Science&Technology Department(No.2023NK2031)the Natural Science Foundation of Hunan Province(No.2023JJ40031)the Ministry of Human Resources and Social Security(No.H20240365).
文摘To explore the adaptive mechanisms of the partial nitritation-anammox(PNA)process under high salinity stress during kitchen wastewater treatment,focusing on their physiological and molecular responses through metagenomic analysis.An airlift inner-circulation partition bioreactor(AIPBR)was developed,featuring an inner cylinder and a flow guide tube to create distinct oxygen gradients,facilitating the study of microbial adaptation under varying salt conditions.The AIPBR was operated with synthetic wastewater containing ammonium concentrations of 1800±100 mg/L and salinity gradients ranging from 1 to 10 g/L,followed by a fixed salinity period at 6 g/L,with ammonium concentrations approximately 850 mg/L.High-throughput metagenomic analysis revealed shifts in functional genes and metabolic pathways in response to salinity stress.Anammox bacteria adapted by enriching genes involved in the synthesis of osmoprotective compounds and activating energy-producing pathways like the tricarboxylic acid cycle(TCA).These adaptations,along with modifications in membrane composition,were essential for sustaining system stability under elevated salinity.Under prolonged high salinity stress,anaerobic ammonium oxidizing(AnAOB)exhibited improved salt tolerance,maintaining a total nitrogen removal efficiency above 85%and stabilizing after an adaptation phase.The metagenomic data revealed a marked enrichment of genes associated with ion transport,stress response mechanisms,and DNA repair pathways.Changes in microbial community composition favored salt-tolerant species,supporting system stability.These findings highlight the applicability of the developed bioreactor for scaling up the PNA process to handle high-salinity wastewater,providing a promising avenue for sustainable nitrogen removal in challenging environments.
基金supported by the China Central Government-Guided Local Science and Technology Development Project(23ZYQA291)the Innovation Star Project for Excellent Postgraduates in Gansu Province(2025CXZX-169)the Key Science&Technology Project of Gansu Province,China(22ZD6NA007)。
文摘Soil water content and salinity critically regulate soil microbial composition,plant community structure,and ecosystem multifunctionality(EMF)in semi-arid grasslands.However,the mechanisms through which drought(D),saline-alkaline(SA),and their combined(DSA)stress influence these ecological components remain poorly understood.This study investigated these mechanisms along natural gradients in a semi-arid grassland of China by analyzing soil physical-chemical properties,microbial communities,and vegetation characteristics.The results showed that as the environmental stress shifted from the D group to the DSA group and then to the SA group,soil electrical conductivity significantly increased,while urease and phosphatase activities significantly decreased.Soil organic carbon,total nitrogen,total phosphorus,and microbial biomass carbon and nitrogen were lower in the D and SA groups than in the DSA group.Meanwhile,plant biomass showed an increasing trend along the treatment gradient,primarily driven by dominant species,while plant diversity did not exhibit significant differences.Further analysis identified the soil water content and salinity as the key determinants of soil microbial diversity and community complexity.Soil enzyme activities exhibited contrasting relationships with microbial composition,correlating positively with the richness of bacterial amplicon sequence variants(ASVs)but negatively with the richness of fungal ASVs.Notably,microbial biomass,which varied significantly across different groups,emerged as a key predictor of changes in EMF,with its critical role confirmed through structural equation modeling.These findings collectively elucidate the responses of ecological communities to synergistic soil hydro-saline stress in semi-arid ecosystems,while highlighting the critical role of microbial biomass in maintaining EMF.
基金funded by the Beef Cattle Research Council Cluster(FDE.18.21C)Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery,NSERC Canadian Research Chair(Tier 1)program+2 种基金NSERC Alliance program(ALLRP 588541‐23)Foundation for Food&Agriculture Research Greener Cattle Initiative(Award ID 22‐000373)DSM Nutritional Products,Kaiseraugst,Switzerland。
文摘Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.
基金financially supported by the National Natural Science Foundation of China(Nos.42225706,42377297,42407408,42177283)the Fundamental Research Funds for the Central Universities of China(No.2662023PY010)the support from the Postdoctoral Fellowship Program of the China Postdoctoral Science Foundation(No.GZB20230246)。
文摘Agricultural ecosystems play a pivotal role in global carbon(C)sequestration efforts.Microbial C use efficiency(CUE)serves as a comprehensive metric that reflects the balance between microbial contributions to the accumulation and decomposition of soil organic C.However,the overall distribution patterns and underlying drivers of microbial CUE at the national scale remain unclear.Herein,data from 209 paired samples from 55 studies were analyzed to assess the distribution patterns and influencing factors of microbial CUE based on enzyme stoichiometry(CUE_(ST))in agricultural ecosystems across China.Results revealed that farmlands exhibited the highest CUE_(ST)value(mean=0.51),exceeding those of grasslands(0.46)and forests(0.44).Contrasting patterns of CUE_(ST)regulation were observed across land-use types,with farmlands showing significant(P<0.001)positive relationships of CUE_(ST)with phosphorus vs.nitrogen(N/P)limitation index,while grasslands and forests demonstrated inverse(P<0.05)relationships of CUE_(ST)with C limitation index.Nutrient stoichiometry emerged as the dominant driver of CUE_(ST),with enzyme ratios and mean annual precipitation playing secondary roles.Moreover,land management practices,including fertilization,grazing,and tillage,as well as land-use transition,significantly influenced microbial CUE_(ST)by potentially altering nutrient availability and soil properties;notably,water addition in grasslands had particularly positive effects.These findings provide a critical foundation for harnessing microbial CUE in agriculture and may inform scalable strategies to enhance soil C sequestration and climate-smart land management.
基金supported by the National Science and Technology Major Program for Noncommunicable Chronic Diseases(2023ZD0503500)the National Natural Science Foundation of China(82030102,12126602,91857118)+1 种基金the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-010,2019-I2M-2-003)the National High Level Hospital Clinical Research Funding(2022-GSP-GG-1,2022-GSP-GG-2)。
文摘Objective Evidence suggests that depleted gut microbialα-diversity is associated with hypertension;however,whether metabolic markers affect this relationship remains unknown.We aimed to determine the potential metabolites mediating the associations ofα-diversity with blood pressure(BP)and BP variability(BPV).Methods Metagenomics and plasma targeted metabolomics were conducted on 523 Chinese participants from the MetaSalt study.The 24-hour,daytime,and nighttime BP and BPV were calculated based on ambulatory BP measurements.Linear mixed models were used to characterize the relationships betweenα-diversity(Shannon and Chao1 index)and BP indices.Mediation analyses were performed to assess the contribution of metabolites to the observed associations.The influence of key metabolites on hypertension was further evaluated in a prospective cohort of 2,169 participants.Results Gut microbial richness(Chao1)was negatively associated with 24-hour systolic BP,daytime systolic BP,daytime diastolic BP,24-hour systolic BPV,and nighttime systolic BPV(P<0.05).Moreover,26 metabolites were strongly associated with richness(Bonferroni P<0.05).Among them,four key metabolites(imidazole propionate,2-hydroxy-3-methylbutyric acid,homovanillic acid,and hydrocinnamic acid)mediated the associations between richness and BP indices(proportions of mediating effects:14.1%–67.4%).These key metabolites were also associated with hypertension in the prospective cohort.For example,each 1-standard deviation unit increase in hydrocinnamic acid significantly reduced the risk of prevalent(OR[95%CI]=0.90[0.82,0.99];P=0.03)and incident hypertension(HR[95%CI]=0.83[0.71,0.96];P=0.01).Conclusion Our results suggest that gut microbial richness correlates with lower BP and BPV,and that certain metabolites mediate these associations.These findings provide novel insights into the pathogenesis and prevention of hypertension.
基金Under the auspices of the National Natural Science Foundation of China(No.42430511,U20A2083)the National Key Research and Development Program of China(No.2022YFF1300900)the Science and Technology Development Program of Jilin Province(No.20210509037RQ,20230101348JC)。
文摘Elevation patterns and assembly processes of soil microbial community structures are essential for understanding biogeo-chemical processes in mountain systems.Differences in soil properties caused by elevation gradients can regulate the spatial distribu-tion and network complexity of the community structure.To explore the variations in soil microbial community structures and their as-sembly mechanisms across different elevations of the Changbai Mountains,as well as their responses to environmental factors,we col-lected microbial samples along an elevational gradient(seven elevations containing four vegetation zones)on the western slope of the Changbai Mountains using the method of metagenomic sequencing.The results showed a significant difference(P<0.05)for the Chao1 index across different elevations,but no significant difference was observed for the Shannon and Simpson indices.With increasing elev-ation,the number of nodes and links in the microbial network gradually decreased.Acidobacteria were highly connected to many nodes.The microbial communities indicated a significant distance-decay relationship(P<0.001)and were affected more by stochastic pro-cesses along the elevation gradient.The results of the Structural Equation Model(SEM)showed that elevation had direct significant ef-fect on carbon(C,P<0.01),nitrogen(N,P<0.01),and phosphorus(P,P<0.05)and weak negative effect on their ecological stoi-chiometry.Elevation was one of the major variables contributing to microbial network topology.The contribution of C and N to micro-bial network complexity was higher than that of P.Our study provides valuable insights into the responses of soil microbial communit-ies to elevation variations.
文摘[Objectives]To analyze the microbial community structure and diversity in the rhizosphere soil of peach trees in the Tangshan area of Hebei Province,identify the dominant microbial groups,and explore their potential ecological functions.[Methods]Amplification sequencing analysis of bacterial and fungal communities in the rhizosphere soil of a peach orchard in Qian'an County,Tangshan City,Hebei Province,was performed using Illumina MiSeq high-throughput sequencing technology.[Results]The indices of Sobs,Chao,ACE,and Shannon for soil bacteria in the rhizosphere soil of peach trees were all higher than those for fungi,indicating a more uniform and diverse bacterial community structure.At the phylum level,the bacteria with relatively high abundance included Pseudomonadota(28.29%),Acidobacteriota(18.10%),Bacillota(12.17%),and Actinomycetota(11.73%).In contrast,the fungi with relatively high abundance were Ascomycota(64.64%),Basidiomycota(14.22%),and Mortierellomycota(14.09%).At the genus level,the bacteria with relatively high abundance comprised Sphingomonas(5.00%),Priestia(3.38%),Nitrospira(2.05%),etc.The fungi with relatively high abundance included Fusarium(13.13%),Mortierella(12.86%),Tausonia(6.97%),Neocosmospora(4.77%),etc.[Conclusions]This study offers a foundational dataset and theoretical reference for the regulation of rhizosphere microecology and the management of soil health in peach orchards in Tangshan.
基金financially supported by the National Natural Science Foundation of China (No. 52371056)the Liaoning Provincial Youth Science Fund Project, China (Category B, No. 2025JH6/101000010)+1 种基金the Guangdong Basic and Applied Basic Research Foundation, China (No. 2024A1515240055)funding from the China Scholarship Council
文摘Microbial contamination and the resulting corrosion in aircraft fuel system pose a serious threat to flight safety.Revealing the corrosion behavior and mechanism of fuel-degrading microorganisms on tank materials is crucial for developing effective mitigation strategies.In this study,the corrosion mechanisms of two representative hydrocarbon-degrading bacteria,Alcanivorax dieselolei and Microbacterium oxydans,toward AA7075 aluminum alloy,were systematically investigated.A combination of biofilm characterization,electrochemical testing,and surface/corrosion product characterization was employed.Both strains markedly accelerated the corrosion of AA7075,as evidence by the progressive decrease in polarization resistance and the pronounced rightward shift of the potentiodynamic polarization curves.Moreover,the difference between the pitting potential(E_(pit))and the corrosion potential(E_(corr))(ΔE=E_(pit)‒E_(corr))decreased due to microbial activities,indicating a pronounced tendency toward accelerated pitting corrosion.Corrosion morphology analysis revealed that both microbes promoted localized pitting corrosion.Furthermore,analysis of aviation kerosene composition indicated that both bacteria accelerated the degradation of C8 and C9 alkanes.These findings highlight the multiple threats of microbial contamination,material degradation,and fuel quality deterioration in fuel systems and underscore the need for targeted protection strategies for marine aviation operations.
基金supported by the Science and Technology Commission of Shanghai Municipality Foundation(No.22230710500)the Interdisciplinary joint research project of Tongji University(No.2023-3-YB-07).
文摘As the core of cathode materials,sensitive metals play important roles in the optimization of acetate production from carbon dioxide(CO_(2))in microbial electrochemical system(MES).In this work,iron(Fe),copper(Cu),and nickel(Ni)as sensitive metal cathode materials were evaluated for CO_(2) conversion in MES.The MES with Feelectrode as a promising electrode material demonstrated a superior CO_(2) reduction performance with a maximum acetate accumulation of 417.9±39.2 mg/L,which was 1.5 and 1.7 folds higher than that in the Ni-electrode and Cu-electrode groups,respectively.Furthermore,an outstanding electron recovery efficiency of 67.7%was shown in the Fe-electrode group.The electron transfer between electrode-suspended sludge was systematically cross-evaluated by the electrochemical behavior and extracellular polymeric substances.The Fe-electrode group had the highest electron transfer rate with 0.194 s-1(k_(app)),which was 17.6 and 21.5 times higher than that of the Cu-and Ni-electrode groups,respectively.Fe-electrode was beneficial for reducing electrochemical impedance between the electrode and suspended sludge.Additionally,redox substances in extracellular polymeric substances of the Fe-electrode group were increased,implying more favorable electron transport dynamics.Simultaneously,enrichments of functional bacteria Acetoanerobium and increased key enzymes involved in the carbonyl pathway of the Fe-electrode group were observed,which also promoted CO_(2) conversion in MES.This study provides a perspective on evaluating the promising sensitive metal electrode material for the process of CO_(2) valorization in MES and offers a reference for the subsequent electrode modification.
基金supported by the National Natural Science Foundation of China(32330105 and 32301247)National Key R&D Program of China(2023YFD1801100)。
文摘Background An imbalance in the rumen microbiota caused by high-concentrate diets(HCD)is a significant endogenous trigger of mastitis.However,the underlying mechanisms remain largely unknown.Microbial extracellular vesicles(mEVs)are critical mediators of microbe-host communication.However,the role of mEVs in rumen microbiota-mediated mastitis has not yet been reported.In this study,we used an HCD-induced rumen microbiota dysbiosis model to investigate the role of mEVs-derived from rumen microbiota in the pathogenesis of mastitis.Results Our results indicate that HCD leads to mastitis and systemic inflammation.Meanwhile,HCD-fed goats exhibited substantial rumen microbiota dysbiosis and the disruption of the rumen barrier.Transplanting rumen microbiota from HCD goats into mice induced both mastitis and systemic inflammation in the recipients.Specifically,HCD increases the production of mEVs carrying microbial DNA,which can translocate across the compromised rumen barrier to the mammary gland,triggering a mammary inflammatory response via activation of the cGAS-STING-NF-κB/NLRP3 pathway.Furthermore,treating mice with mEVs isolated from the rumen fluid of HCD goats directly induced mastitis,whereas depletion of microbial DNA attenuated mEVs-induced mastitis.Conclusion Our findings suggest that HCD induces rumen microbiota dysbiosis and impairs rumen barrier function.This dysfunction leads to an increase in microbial DNA-containing mEVs,which subsequently leak into the mammary gland.Once there,these mEVs activate the cGAS-STING-NF-κB/NLRP3 signaling pathway,ultimately inducing mastitis.This study provides a new perspective on the“rumen microbiota-mammary gland axis”and enhances the understanding of the pathogenesis of mastitis.
基金supported by the National Key Research and Develop-ment Program of China(No.2021YFC3200805)the National Natu-ral Science Foundation of China(Nos.52325001 and 52170009).
文摘Liquid-solid phase transfer promotes the interaction of perfluoroalkyl acids(PFAAs)with the microbial system of river sediments,which may affect the environmental behavior of antibiotic resistance genes(ARGs)contained in benthic environments.Sediments collected from the receiving water of the largest fluoropolymer production facility in China were analyzed to investigate the impact of PFAAs on microbial communities and ARG profiles.The main contributors to the PFAAs were perfluorooctanoic acid and perfluorobutanoic acid,whose proportions(86.9%-93.4%)in the downstream surface sediments affected by industrial effluents were significantly higher than in the corresponding upstream samples(53.3%).A reduction in microbial diversity and richness was observed in the presence of high concentrations of PFAAs at the downstream sites.144 ARG subtypes,including three high-risk subtypes(bacA,aac(6′)-I and aadA),were identified in sediment samples.The discharge of fluorochemical effluents also results in a reduction of ARG diversity at subtype level.PFAAs exert a pronounced influence on the profile of ARGs in sediment.PFAAs and water quality parameters(e.g.pH and total phosphorus)were key drivers of the microbial community composition in the sediment.The regulation of microbial communities by PFAAs may represent an important pathway by which these compounds affect ARG profiles.
基金supported by the National Natural Science Foundation of China(No.42377083)the Natural Science Foundation of Sichuan Province,China(No.2025 ZNSFSC0433).
文摘In this work,ofloxacin(OFL),a kind of frequently detected antibiotic in groundwater,was selected to explore its impact(at ng/L-μg/L-level)on denitrification performance in an autotrophic denitrification system driven by pyrite/sulfur(FeS2/S0).Results showed that OFL restrained nitrate removal efficiency,and the inhibition degree was positively related to the concentration of OFL.After being exposed to increased OFL(200 ng/L-100μg/L)for 69 days,higher inhibition of electron transport activity(ETSA),enzyme activities of nitrate reductase(NAR),and nitrite reductase(NIR)were acquired.Meanwhile,the extracellular protein(PN)content of sludge samples was remarkably stimulated by OFL to resist the augmented toxicity.OFL contributed to increased microbial diversity and sulfur/sulfide oxidation functional genes in ng/L-level bioreactors,whereas led to a decline inμg/L level experiments.With OFL at concentrations of 200 ng/L and 100μg/L,the whole expression of 10 key denitrification functional genes was depressed,and the higher the OFL concentration,the lower the expression level.However,no significant proliferation of antibiotic resistance genes(ARGs)either in 200 ng/L-OFL or 100μg/L-OFL groups was observed.Two-factor correlation analysis results indicated that Thiobacillus,Anaerolineae,Anaerolineales,and Nitrospirae might be the main hosts of existing ARGs in this system.
基金supported by the National Natural Science Foundation of China(grant no.U23A2016 and 42101094).
文摘Land-use change and associated landscape pattern modifications are key drivers of biodiversity dynamics.However,the influence of landscape heterogeneity on soil microbial diversity remains poorly understood.This study analyzed soil microbial communities(bacteria and fungi)and soil properties(organic carbon,pH,available potassium,nitrogen,and phosphorus)across 74 sites in Hainan,China,covering six land-use types(rubber,areca,banana,farmland,longan,and mango).We evaluated the effects of both landscape composition(habitat coverage and patch richness)and configuration heterogeneity(patch density and largest patch index)on soil microbial community structure and diversity.Results show that land-use types and landscape patterns weakly affect microbial composition but strongly shape diversity in a taxon-specific way.Bacterial Chao1 richness and fungal Shannon diversity varied significantly among land-use types,mainly due to soil available phosphorus.Rubber coverage positively correlated with bacterial and fungal diversity,while the largest patch index negatively affected only fungal diversity.Available phosphorus reduced this negative effect.This study demonstrates that land-use change and landscape heterogeneity differentially influence soil microbial composition and diversity,with available phosphorus emerging as a key determinant.Our findings highlight the need for careful landscape planning and soil nutrient management to preserve soil microbial diversity.
基金supported by the National Natural Science Foundation of China(No.42077135).
文摘Heavy metal contamination of soil is one of the major challenges to sustainable agriculture.This contamination can be transmitted through the soil food chain and poses a serious threat to human health.In this study,we found that freeze-thaw leaching(FTL)effectively complements the low removal rate of chemical leaching,and investigated the effects of different numbers of FTL on Cd contamination,soil chemical properties and microbial communities.The results showed that repeated FTL significantly reduced(P<0.05)the total Cd content in the top soil(19.02%-49.35%)and subsoil(0.41%-21.13%)and promoted the transformation of Cd to a more stable form,mainly through various removal mechanisms such as complexation,ion-exchange,and chemical precipitation.This finding was supported by reductions in several soil properties,including pH,available potassium(AK),and available phosphorus(AP).FTL treatment initially increased the bioavailability of Cd compared to chemical leaching,but bioavailability of Cd progressively decreased as the number of freeze-thaw cycles increased.Additionally,FTL reduced the richness and diversity of bacteria communities,destabilized ecological symbiotic networks,while increasing the richness and diversity of fungi in the soil.Various model analyses indicated that FTL treatment,available Cd,soil pH,AP and AK were the key drivers influencing the changes in microbial community structure.This study provides new insights and scientific bases for the effective management of heavy metal pollution in agricultural soils,the restoration of ecosystem health,and the improvement of soil sustainability.
基金funded by the National Natural Science Foundation of China(No.42402310)the Natural Science Foundation of Henan Province(No.242300421657)+1 种基金the National Key Research and Development Program(No.2024YFD2402005)the China Agriculture Research System(No.CARS-50)。
文摘Sedimentary microbial communities play an important role in driving biogeochemical cycles in river ecosystems.The Yellow River is one of the rivers with the highest turbidity over the world.However,limited is known about the microbial variation and its influencing factors in the Yellow River.In this study,we examined the microbial communities and their influencing factors in the sediment of Upper and Mid–Lower reaches of the Yellow River.The results showed that Gammaproteobacteria were most dominant(with Hydrogenophilaceae being the predominant family)in the studied Yellow River sediments.Phyla of Deltaproteobacteria,Nitrospirae and family of Bacillaceae,Geobaceraceae were more abundant in the Mid–Lower reaches than in the Upper reaches,while phyla of Gammaproteobacteria,Verrucomicrobia and family of Caldilineaceae,Llumatobacteraceae were more abundant in the Upper reaches than in the Mid–Lower reaches.The microbial communities were predominantly affected by nutrient factors(such as NH_(4)^(+),TN and TP),followed by the spatial and the content of Chla in the Mid–Lower reaches,while they were by predominantly affected by spatial factors,followed by the nutrient factors and the content of Chla in the Upper reaches.The dominant microbial taxa were mostly correlated with COD,NH_(4)^(+),TP and temperature,but they responded differently to these physiochemical factors between the Upper and Mid–Lower reaches.In summary,the sedimentary microbial communities differ between the Upper and Mid–Lower reaches and respond differently to the environmental and spatial factors in the sediment of the Yellow River.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFC3707900)National Natural Science Foundation of China(Grant No.42230710,42525201)Key task project for joint research and development of the Yangtze River Delta Science and Technology Innovation Community(Grant No.2022CSJGG1200).
文摘Microbially induced calcium carbonate precipitation(MICP)is an eco-friendly technology for soil improvement.Although numerous experiments have been conducted to solidify sand foundations using MICP,the mechanisms by which grain interfacial morphologies influencethe MICP process remain unclear.This study utilized 3D-printed flowcells with different boundary morphologies to investigate the effects of interfacial morphologies on the MICP process.CaCO_(3)precipitation characteristics were investigated through microscopic observation and image quantificationanalysis.The results indicate that low flowvelocities near the interface promote bacterial accumulation due to reduced hydrodynamic shear forces.Rough interfaces,compared to smooth ones,enhance bacterial adsorption owing to the larger regions of low flowvelocity,increased surface area,and the formation of local eddies,which promote greater CaCO_(3)precipitation.Compared to the regions away from the interface,a higher abundance of small CaCO_(3)crystals is observed near the interface because of the high urease activity from bacteria and the reduced shear-induced entrainment due to the low flowvelocity.Besides,larger crystals also preferentially precipitate in proximity to interfaces as the low flowvelocity enhances crystal growth according to the particle attachment theory.The presence of rough interfaces further reduces flowvelocities,leading to the precipitation of larger and more densely packed CaCO_(3)crystals.Therefore,rough interfaces promote the microbially induced calcium carbonate precipitation.This work is expected to enhance the understanding of microbially induced calcium carbonate precipitation characteristics on solid surfaces such as soil grains and contribute to the optimization of MICP applications.
基金the Department of Science and Technology(DST)-India for providing a departmental grant to the Department of Biochemistry,Central University of Punjab,Bathinda,India,in the form of a DST-Fund for Improvement of S&T Infrastructure(FIST)grant。
文摘Microplastics (MPs), fluoride (FR), and fungicide mancozeb (MZ) are common contaminants in soil. However, there is no information about the combined impacts of MPs, FR, and MZ on tomato plant growth features in the literature. This study aimed to investigate the effects of combined application of MPs, FR, and MZ (both analytical grade and commercial) on the growth and development of tomato plants and metagenomics of rhizosphere soil. A pot experiment was set up in an artificial greenhouse with two sets of treatments. One set was the combined application of MPs, FR, and analytical grade MZ (B2) and a control without the application of MPs, FR, and MZ (W2), and the other set was the combined application of MPs, FR, and commercial MZ (B3) and a control without the application of MPs, FR, and MZ (W3). No detrimental effects of MPs, FR, and MZ were detected on the growth parameters of tomato plants, including the number of leaves and chlorophyll concentration. However, tomato roots showed knot and nodulation-type structures, and metabolomic profiling revealed that combined exposure to MPs, FR, and MZ profoundly reprogrammed the primary metabolism in tomato roots, with marked alterations in carbohydrate and amino acid pathways. Metagenome whole genome sequencing showed that the B2 and B3 treatments profoundly affected soil microbial community composition, diversity, gene abundances, and functional gene variations compared to W2 and W3. Proteobacteria became the dominating phylum in B2 and B3, causing a significant shift in the microbiome. Its abundance soared to 66.7% in B2 and 75.4% in B3, compared to only 35.9% in W2 and 28.9% in W3. On the other hand, Actinobacteria decreased significantly from 55.6% in W2 and 63.8% in W3 to 18.1% in B2 and 9.6% in B3. This study highlights the microbial shifts due to combined application of MPs, FR, and MZ, providing evidence for understanding their environmental risks.
