Background:The major histocompatibility complex class I chain-related protein B(MICB)gene,part of the non-classical human leukocyte antigen(HLA)class I family,plays an important role in tumor escaping immune surveilla...Background:The major histocompatibility complex class I chain-related protein B(MICB)gene,part of the non-classical human leukocyte antigen(HLA)class I family,plays an important role in tumor escaping immune surveillance.Methods:This study used bioinformatics to assess MICB’s diagnostic and prognostic value across various cancers and its role in liver cancer drug resistance and immune escape.Immunohistochemistry and western blotting were used to explore MICB expression in liver cancer tissues and adjacent tissues(para-carcinoma tissue).Results:MICB showed diagnostic value for 15 cancer types and prognostic value for two.Its expression correlated with several anticancer drugs,including fludarabine.Identifying the ceRNA regulatory network could speed up hepatocellular carcinoma(HCC)development and immune evasion.The study observed elevated MICB protein levels in liver cancer tissues relative to para-carcinoma tissue,and noted that the HCC cell line,HLE,exhibited high sensitivity to fludarabine.Conclusion:MICB shows diagnostic and prognostic potential in various cancers,with high expression possibly linked to fludarabine resistance.The SNHG14-hsa-miR-122-5p-MICB ceRNA network may significantly influence HCC progression and immune escape.展开更多
目的MHCI类相关基因（MHC class Ⅰ chain-related gene，MIC）与MHCI类基因连锁，同源性很高。我们的前期研究发现MICA和MICB微卫星多态性与溃疡性结肠炎（UC）相关，本研究继续探讨MICB第2、3、4外显子基因多态性与UC的相关性，以期发...目的MHCI类相关基因（MHC class Ⅰ chain-related gene，MIC）与MHCI类基因连锁，同源性很高。我们的前期研究发现MICA和MICB微卫星多态性与溃疡性结肠炎（UC）相关，本研究继续探讨MICB第2、3、4外显子基因多态性与UC的相关性，以期发现UC的易感基因。方法采用聚合酶链反应．单链构象多态性方法（PCR-SSCP），对105例湖北汉族UC患者和213例正常对照者进行MICB基因分型。结果UC患者的MICB 0106等位基因频率较正常组显著增高（19．0％比8．9％，P=0．000，Pc〈0．001，OR=2．402，95％CI为1．488～3．879）。在临床亚型分析中，MICB0106等位基因频率在全结肠炎、中重度UC组及有肠外表现的UC患者中均显著增高（分别为24．4％比8．9％，P=0．000，Pc〈0．001，OR=3．294，95％CI为1．800～6．027；24．1％比8．9％，P=0．000，Pc〈0．001，OR=3．249，95％CI为1．893～5．576；20．5％比8．9％，P=0．002，Pc=0．012，OR=2．626，95％CI为1．418～4．861）。而且MICB0106等位基因频率在男性和年龄≥40岁的患者中比正常组显著增高（分别为22．1％比8．0％，P=0．001，Pc=0．006，OR=3．276，95％CI为1．737～6．178；28．8％比8．3％，P=0．000，Pc〈0．001，OR=4．500，95％CI为2．381～8．504）。结论MICB0106等位基因与湖北汉族人UC存在显著相关性，而且与全结肠炎、中重度UC、肠外表现、男性及年龄≥4JD岁的患者有关，提示携带MICB0106等位基因的个体对UC存在遗传易感性。展开更多
基金supported by the National Natural Science Foundation of China(Nos.82460602,82060514,82560459,and 82573045)the Natural Science Foundation of Hainan Province(Nos.824RC517 and 822RC700)+2 种基金the Hainan Province Science and Technology Special Foundation(No.ZDYF2021SHFZ222)the Hainan Medical University Natural Science Foundation(No.JBGS202106)the Hainan Provincial Association for Science and Technology,Program of Youth Science Talent and Academic Innovation(No.QCXM201922).
文摘Background:The major histocompatibility complex class I chain-related protein B(MICB)gene,part of the non-classical human leukocyte antigen(HLA)class I family,plays an important role in tumor escaping immune surveillance.Methods:This study used bioinformatics to assess MICB’s diagnostic and prognostic value across various cancers and its role in liver cancer drug resistance and immune escape.Immunohistochemistry and western blotting were used to explore MICB expression in liver cancer tissues and adjacent tissues(para-carcinoma tissue).Results:MICB showed diagnostic value for 15 cancer types and prognostic value for two.Its expression correlated with several anticancer drugs,including fludarabine.Identifying the ceRNA regulatory network could speed up hepatocellular carcinoma(HCC)development and immune evasion.The study observed elevated MICB protein levels in liver cancer tissues relative to para-carcinoma tissue,and noted that the HCC cell line,HLE,exhibited high sensitivity to fludarabine.Conclusion:MICB shows diagnostic and prognostic potential in various cancers,with high expression possibly linked to fludarabine resistance.The SNHG14-hsa-miR-122-5p-MICB ceRNA network may significantly influence HCC progression and immune escape.
