Essential oil from seeds of Foeniculum vulgare was extracted on Clevenger apparatus. Essential oil was analyzed on Gas-Chromatography-Mass spectrometry (GC-MS) from which thirty six components were identified, among w...Essential oil from seeds of Foeniculum vulgare was extracted on Clevenger apparatus. Essential oil was analyzed on Gas-Chromatography-Mass spectrometry (GC-MS) from which thirty six components were identified, among which 6 major and 30 minor components having different structural formulae and molecular weight representing total 99.98% of oil. Essential was investigated for its antibacterial and antifungal activity against seven infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. The Foeniculum vulgare essential oil showed the Diameter of Inhibition Zone (DIZ) ranging from 19.4 ± 0.07 - 26.4 ± 0.09 mm at a concentration level of 28 μg/disc in all the ten strains tested. The minimum inhibitory concentration (MIC) of essential oil against bacterial and fungal strains was obtained in the range of 7.0 - 56 μg/ml. Antibacterial and antifungal activity of Foeniculum vulgare essential oil is due to the presence of certain secondary plant metabolites such as terpenoids, steroids and flavonoids, esters and acids which are identified in the essential oil. The oil components can be further studied for their biological activity and overcome the problem of drug resistance in microbes.展开更多
Aims: The aim of this undertaken investigation was designed to determine the comparative antimicrobial potential of ethanol extract of six commonly consumed spices such as Garlic (Allium satilyvum), Ginger (Zingiber o...Aims: The aim of this undertaken investigation was designed to determine the comparative antimicrobial potential of ethanol extract of six commonly consumed spices such as Garlic (Allium satilyvum), Ginger (Zingiber officinale), Turmeric (Curcuma longa), Cinnamon (Cinnamomum zeylanicum), Cumin (Cuminum cyminum) and Black cumin (Nigella sativa). Method: This study includes, the efficacy of individual and synergistic effect of these extracts that was tested against bacteria by agar well-diffusion method employing 100 μL spices-extract solution per well and was conducted in (Centre of Excellence Laboratory) Department of Microbiology, Primeasia University during November 2018 to April 2019. Minimum inhibitory concentration (MIC) was determined by the micro-broth dilution method and compared with commercial antibiotic discs such as Amoxicillin, Vancomycin, Erythromycin, Ceftriaxone, Chloramphenicol, and Ciprofloxacin. Result: According to the findings of the antibacterial assay, the ethanol extracts of the spices showed inhibitory activity against common infectious bacterial pathogens. Spice extracts have the most significant activity against B. cereus and E. coli was the least sensitive among the tested organisms. The ethanol extract had individual antibacterial activity with mean zone of inhibition 22 ± 0.5 and 20.08 ± 0.58 mm and the synergistic effect of ethanol extract had a mean zone of inhibition 30 ± 0.75 and 28.25 ± 0.9 mm against B. cereus and V. cholera, respectively, which is highly comparable to the commercial antibiotic, Ciprofloxacin (25 mm). Conclusion: The ethanol extract of indigenous spices was shown to be highly potential to be applied as an alternative of commercial drugs.展开更多
The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span s...The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span style="font-family:Verdana;">L</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">monocitogenes</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;"> is considered one of the most important pathogens responsible for food-borne infection. Antimicrobial properties were evaluated using the standard Kirby-Bauer disk diffusion method. Bacterial inactivation networks were determined and compared, as well as the possibility of using powders and extracts of berries to control the risk of </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogees</span></i><span style="font-family:Verdana;"> infestation in the milk and dairy industry as well as in the meat industry. The effect of pH (4.78 - 4.43) and water activity (0.90 - 0.80) on the relationship between optical density (OD) at 600 nm and the plate count (CFU ml<sup>-</sup></span><span style="font-family:Verdana;"><sup>1</sup></span><span style="font-family:Verdana;">) was investigated for </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;">. It was determined Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) </span><span style="font-family:Verdana;">of berries for</span><span style="font-family:;" "=""> </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">The most relevant bacteriostatic and bactericidal </span><span style="font-family:;" "=""><span style="font-family:Verdana;">effect on </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;"> in the tested berries demonstrated sea buckthorn and rosehip.</span></span>展开更多
文摘Essential oil from seeds of Foeniculum vulgare was extracted on Clevenger apparatus. Essential oil was analyzed on Gas-Chromatography-Mass spectrometry (GC-MS) from which thirty six components were identified, among which 6 major and 30 minor components having different structural formulae and molecular weight representing total 99.98% of oil. Essential was investigated for its antibacterial and antifungal activity against seven infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. The Foeniculum vulgare essential oil showed the Diameter of Inhibition Zone (DIZ) ranging from 19.4 ± 0.07 - 26.4 ± 0.09 mm at a concentration level of 28 μg/disc in all the ten strains tested. The minimum inhibitory concentration (MIC) of essential oil against bacterial and fungal strains was obtained in the range of 7.0 - 56 μg/ml. Antibacterial and antifungal activity of Foeniculum vulgare essential oil is due to the presence of certain secondary plant metabolites such as terpenoids, steroids and flavonoids, esters and acids which are identified in the essential oil. The oil components can be further studied for their biological activity and overcome the problem of drug resistance in microbes.
文摘Aims: The aim of this undertaken investigation was designed to determine the comparative antimicrobial potential of ethanol extract of six commonly consumed spices such as Garlic (Allium satilyvum), Ginger (Zingiber officinale), Turmeric (Curcuma longa), Cinnamon (Cinnamomum zeylanicum), Cumin (Cuminum cyminum) and Black cumin (Nigella sativa). Method: This study includes, the efficacy of individual and synergistic effect of these extracts that was tested against bacteria by agar well-diffusion method employing 100 μL spices-extract solution per well and was conducted in (Centre of Excellence Laboratory) Department of Microbiology, Primeasia University during November 2018 to April 2019. Minimum inhibitory concentration (MIC) was determined by the micro-broth dilution method and compared with commercial antibiotic discs such as Amoxicillin, Vancomycin, Erythromycin, Ceftriaxone, Chloramphenicol, and Ciprofloxacin. Result: According to the findings of the antibacterial assay, the ethanol extracts of the spices showed inhibitory activity against common infectious bacterial pathogens. Spice extracts have the most significant activity against B. cereus and E. coli was the least sensitive among the tested organisms. The ethanol extract had individual antibacterial activity with mean zone of inhibition 22 ± 0.5 and 20.08 ± 0.58 mm and the synergistic effect of ethanol extract had a mean zone of inhibition 30 ± 0.75 and 28.25 ± 0.9 mm against B. cereus and V. cholera, respectively, which is highly comparable to the commercial antibiotic, Ciprofloxacin (25 mm). Conclusion: The ethanol extract of indigenous spices was shown to be highly potential to be applied as an alternative of commercial drugs.
文摘The purpose of this study was to first evaluate the antimicrobial effects of powder and extracts of berries (rose-hip, aronia, sea buckthorn and hawthorn) on the development of antibiotic-resistant <i><span style="font-family:Verdana;">L</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">monocitogenes</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;"> is considered one of the most important pathogens responsible for food-borne infection. Antimicrobial properties were evaluated using the standard Kirby-Bauer disk diffusion method. Bacterial inactivation networks were determined and compared, as well as the possibility of using powders and extracts of berries to control the risk of </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogees</span></i><span style="font-family:Verdana;"> infestation in the milk and dairy industry as well as in the meat industry. The effect of pH (4.78 - 4.43) and water activity (0.90 - 0.80) on the relationship between optical density (OD) at 600 nm and the plate count (CFU ml<sup>-</sup></span><span style="font-family:Verdana;"><sup>1</sup></span><span style="font-family:Verdana;">) was investigated for </span><i><span style="font-family:Verdana;">Listeria</span></i> <i><span style="font-family:Verdana;">monocytogenes</span></i><span style="font-family:Verdana;">. It was determined Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) </span><span style="font-family:Verdana;">of berries for</span><span style="font-family:;" "=""> </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">The most relevant bacteriostatic and bactericidal </span><span style="font-family:;" "=""><span style="font-family:Verdana;">effect on </span><i><span style="font-family:Verdana;">L. monocytogenes</span></i><span style="font-family:Verdana;"> in the tested berries demonstrated sea buckthorn and rosehip.</span></span>
