Molecular genetic polymorphisms were studied for MHC–B–L IⅡß(β1 exon)locus in ten breeds of chicken by PCR-RFLPs.Thirty-seven genotypes were detectedin these chicken.In the 305bp fragment derived from MHC-B-L...Molecular genetic polymorphisms were studied for MHC–B–L IⅡß(β1 exon)locus in ten breeds of chicken by PCR-RFLPs.Thirty-seven genotypes were detectedin these chicken.In the 305bp fragment derived from MHC-B-LIⅡβ(β,exon)locusdigested with Hha I,EcoRV,HaeⅢ,Xba I,there were significent difference with thefrequencies of genotype and combined genotype among individuals or breeds.Statistical results showed Hardy-Weinberg equilibrium in polymorphic sites wereinconsistent among various breeds.Sequencing results suggested there are morepolymorphism at the level of amino acid than at that of nucleotide.Furthermore,asantigen binding site,the highly polymorphisms of 8,exon sequence was a reflectionto the antigen diversity of immune systems.Moreover,it was implied that there washighly association between genetic polymorphisms of B exon and reproductionperformance in chicken.展开更多
文摘Molecular genetic polymorphisms were studied for MHC–B–L IⅡß(β1 exon)locus in ten breeds of chicken by PCR-RFLPs.Thirty-seven genotypes were detectedin these chicken.In the 305bp fragment derived from MHC-B-LIⅡβ(β,exon)locusdigested with Hha I,EcoRV,HaeⅢ,Xba I,there were significent difference with thefrequencies of genotype and combined genotype among individuals or breeds.Statistical results showed Hardy-Weinberg equilibrium in polymorphic sites wereinconsistent among various breeds.Sequencing results suggested there are morepolymorphism at the level of amino acid than at that of nucleotide.Furthermore,asantigen binding site,the highly polymorphisms of 8,exon sequence was a reflectionto the antigen diversity of immune systems.Moreover,it was implied that there washighly association between genetic polymorphisms of B exon and reproductionperformance in chicken.
