目的:探讨组蛋白变异体macroH2A(mH2A)和Butein共同作用GRP78蛋白通过丝裂原激活蛋白激酶(mitogen-activation protein kinase,MAPK)信号通路调控黑色素瘤的生物学行为。方法:采用免疫组织化学检测黑色素瘤和癌旁正常组织中mH2A和葡萄...目的:探讨组蛋白变异体macroH2A(mH2A)和Butein共同作用GRP78蛋白通过丝裂原激活蛋白激酶(mitogen-activation protein kinase,MAPK)信号通路调控黑色素瘤的生物学行为。方法:采用免疫组织化学检测黑色素瘤和癌旁正常组织中mH2A和葡萄糖调节蛋白78(glucose regulated protein of 78,GRP78)的表达情况;分析临床资料与mH2A和GRP78表达的关系;免疫共沉淀实验检测GRP78和mH2A蛋白的相互作用;Western印迹检测mH2A和GRP78的相关作用及Butein与mH2A之间的作用。Transwell试验检测mH2A的表达对黑色素瘤细胞侵袭能力的影响;划痕试验检测mH2A的表达对黑色素瘤细胞迁移能力的影响;Western印迹检测沉默mH2A后细胞外信号调节激酶1(ERK1)和MAPK/ERK激酶(MEK)蛋白的表达。结果:与癌旁正常组织相比,黑色素瘤组织中mH2A和GRP78表达明显降低(P<0.05);Butein可以增强mH2A的表达水平(P<0.05),mH2A可以调节GRP78蛋白的表达(P<0.05);沉默mH2A促进黑色素瘤A375细胞的侵袭迁移能力,上调MEK和ERK1蛋白的表达。结论:Butein可以增强mH2A靶向GRP78蛋白通过MAPK信号通路抑制黑色素瘤细胞的迁移和侵袭行为。展开更多
The decapentaplegic(Dpp),a member of the TGF-β superfamily,plays a pivotal role in the control of proliferation,global patterning and induction of specific cell fates during Drosophila development.Mother against Dpp(...The decapentaplegic(Dpp),a member of the TGF-β superfamily,plays a pivotal role in the control of proliferation,global patterning and induction of specific cell fates during Drosophila development.Mother against Dpp(Mad) is the founding member of the conserved Smad protein family which spe-cifically transduces the intracellular TGF-β signaling cascade.Here we report the 2.80 structure of the MH2 domain of Mad(Mad-MH2) that was readily superposed to the mammal Smad-MH2 structures.This unphosphorylated Mad-MH2 forms a symmetric homotrimer in crystals,consistent with the result of the size-exclusion chromatography that Mad-MH2 exhibited a propensity for concentration-dependent oligomerization prior to phosphorylation.Structural analysis revealed that the formation of homotrimeric Mad-MH2 is mainly mediated by contacts involving the extreme C-terminal SSVS motif,and is strengthened by phosphorylation of the last two Ser residues which was confirmed by the gel filtration analysis of the pseudophosphorylated Mad-MH2(DVD).Intriguingly,the homotrimer within an asymmetric unit only possesses two ordered C-terminal tails,reminiscent of the arrangement of the R-Smad/Smad4 complexes,indicating that the subunit with a flexible SSXS motif would be readily replaced by Co-Smad to form a functional heterotrimer.展开更多
Objective: the elderly patients with hypothyroidism in the treatment of depression when the use of comprehensive nursing intervention, to observe the nursing effect. Methods: 72 cases of middle-aged and elderly patien...Objective: the elderly patients with hypothyroidism in the treatment of depression when the use of comprehensive nursing intervention, to observe the nursing effect. Methods: 72 cases of middle-aged and elderly patients with hypothyroidism in our department from January 2020 to January 2021 were taken as the observation objects, and divided into group a and group b, using basic nursing intervention and using comprehensive nursing intervention respectively. Results: after nursing, SAS score and SDS score in group a and group b were 54.69 ± 4.51 (points) and 58.19±4.92 (points) respectively in group a and 40.16±3.86 (points) and 48.27±4.66 (points) in group b (t=11.454, P =0.000). The results were different. Conclusion: comprehensive nursing intervention in the treatment of depression in middle-aged and elderly patients with hypothyroidism has good effect, and this method can be popularized.展开更多
Objective: to explore the causes of venous thrombosis in patients with tumor chemotherapy after PICC catheterization, and the therapeutic effect after certain nursing intervention. Methods: 119 cases of PICC catheteri...Objective: to explore the causes of venous thrombosis in patients with tumor chemotherapy after PICC catheterization, and the therapeutic effect after certain nursing intervention. Methods: 119 cases of PICC catheterization patients admitted to the hospital from January 2020 to August 2020 were selected as the research object, and they were randomly divided into two groups, control group and observation group, 59 patients in the control group and 60 patients in the observation group. After PICC catheterization in the observation group and the control group, the observation group was given certain nursing intervention measures, while the control group was given conventional nursing measures. After a period of treatment, the nursing intervention effect of the two groups of patients was judged by the number of cases of venous thrombosis formed after PICC replacement in the two groups of patients. Results: two groups of patients after accept certain nursing measures, observation group of patients with tumor in PICC catheter in 5 patients with venous thrombosis after the operation, the control group patients with tumor in 8 cases of venous thrombosis after PICC catheter surgery patients, by comparison, the observation group of patients with tumor in PICC catheter for venous thrombosis after the operation, the patients with tumor in probability is lower than the control group, the difference was statistically significant (P < 0.05). Conclusion: certain nursing intervention measures should be taken for tumor chemotherapy patients after PICC catheterization, which can reduce the probability of venous thrombosis after operation, and can be widely applied in clinical treatment.展开更多
文摘目的:探讨组蛋白变异体macroH2A(mH2A)和Butein共同作用GRP78蛋白通过丝裂原激活蛋白激酶(mitogen-activation protein kinase,MAPK)信号通路调控黑色素瘤的生物学行为。方法:采用免疫组织化学检测黑色素瘤和癌旁正常组织中mH2A和葡萄糖调节蛋白78(glucose regulated protein of 78,GRP78)的表达情况;分析临床资料与mH2A和GRP78表达的关系;免疫共沉淀实验检测GRP78和mH2A蛋白的相互作用;Western印迹检测mH2A和GRP78的相关作用及Butein与mH2A之间的作用。Transwell试验检测mH2A的表达对黑色素瘤细胞侵袭能力的影响;划痕试验检测mH2A的表达对黑色素瘤细胞迁移能力的影响;Western印迹检测沉默mH2A后细胞外信号调节激酶1(ERK1)和MAPK/ERK激酶(MEK)蛋白的表达。结果:与癌旁正常组织相比,黑色素瘤组织中mH2A和GRP78表达明显降低(P<0.05);Butein可以增强mH2A的表达水平(P<0.05),mH2A可以调节GRP78蛋白的表达(P<0.05);沉默mH2A促进黑色素瘤A375细胞的侵袭迁移能力,上调MEK和ERK1蛋白的表达。结论:Butein可以增强mH2A靶向GRP78蛋白通过MAPK信号通路抑制黑色素瘤细胞的迁移和侵袭行为。
基金Supported by National Key Basic Research and Development Program of China (Grant Nos. 2006CB503900 and 2007CB914400)China National Funds for Distinguished Young Scientists (Grant No. 30425005)
文摘The decapentaplegic(Dpp),a member of the TGF-β superfamily,plays a pivotal role in the control of proliferation,global patterning and induction of specific cell fates during Drosophila development.Mother against Dpp(Mad) is the founding member of the conserved Smad protein family which spe-cifically transduces the intracellular TGF-β signaling cascade.Here we report the 2.80 structure of the MH2 domain of Mad(Mad-MH2) that was readily superposed to the mammal Smad-MH2 structures.This unphosphorylated Mad-MH2 forms a symmetric homotrimer in crystals,consistent with the result of the size-exclusion chromatography that Mad-MH2 exhibited a propensity for concentration-dependent oligomerization prior to phosphorylation.Structural analysis revealed that the formation of homotrimeric Mad-MH2 is mainly mediated by contacts involving the extreme C-terminal SSVS motif,and is strengthened by phosphorylation of the last two Ser residues which was confirmed by the gel filtration analysis of the pseudophosphorylated Mad-MH2(DVD).Intriguingly,the homotrimer within an asymmetric unit only possesses two ordered C-terminal tails,reminiscent of the arrangement of the R-Smad/Smad4 complexes,indicating that the subunit with a flexible SSXS motif would be readily replaced by Co-Smad to form a functional heterotrimer.
文摘Objective: the elderly patients with hypothyroidism in the treatment of depression when the use of comprehensive nursing intervention, to observe the nursing effect. Methods: 72 cases of middle-aged and elderly patients with hypothyroidism in our department from January 2020 to January 2021 were taken as the observation objects, and divided into group a and group b, using basic nursing intervention and using comprehensive nursing intervention respectively. Results: after nursing, SAS score and SDS score in group a and group b were 54.69 ± 4.51 (points) and 58.19±4.92 (points) respectively in group a and 40.16±3.86 (points) and 48.27±4.66 (points) in group b (t=11.454, P =0.000). The results were different. Conclusion: comprehensive nursing intervention in the treatment of depression in middle-aged and elderly patients with hypothyroidism has good effect, and this method can be popularized.
文摘Objective: to explore the causes of venous thrombosis in patients with tumor chemotherapy after PICC catheterization, and the therapeutic effect after certain nursing intervention. Methods: 119 cases of PICC catheterization patients admitted to the hospital from January 2020 to August 2020 were selected as the research object, and they were randomly divided into two groups, control group and observation group, 59 patients in the control group and 60 patients in the observation group. After PICC catheterization in the observation group and the control group, the observation group was given certain nursing intervention measures, while the control group was given conventional nursing measures. After a period of treatment, the nursing intervention effect of the two groups of patients was judged by the number of cases of venous thrombosis formed after PICC replacement in the two groups of patients. Results: two groups of patients after accept certain nursing measures, observation group of patients with tumor in PICC catheter in 5 patients with venous thrombosis after the operation, the control group patients with tumor in 8 cases of venous thrombosis after PICC catheter surgery patients, by comparison, the observation group of patients with tumor in PICC catheter for venous thrombosis after the operation, the patients with tumor in probability is lower than the control group, the difference was statistically significant (P < 0.05). Conclusion: certain nursing intervention measures should be taken for tumor chemotherapy patients after PICC catheterization, which can reduce the probability of venous thrombosis after operation, and can be widely applied in clinical treatment.
