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小鼠胎肝基质细胞系MFLC的建立及鉴定
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作者 刘倩 曹良弦 +2 位作者 刘祝公 吴江声 陈慰峰 《解剖学报》 CAS CSCD 北大核心 1997年第4期400-403,I012,共5页
用体外长期培养的方法建立了小鼠胎肝基质细胞系(murinefetallivercel,MFLC),并运用免疫荧光技术、电镜技术、倍增时间的测定及染色体检查等方法,对此细胞系进行了细胞学鉴定。此细胞系建立至今已2年,历... 用体外长期培养的方法建立了小鼠胎肝基质细胞系(murinefetallivercel,MFLC),并运用免疫荧光技术、电镜技术、倍增时间的测定及染色体检查等方法,对此细胞系进行了细胞学鉴定。此细胞系建立至今已2年,历经3个阶段。细胞为多角形,用免疫荧光法显示胞浆内含角蛋白,同时也表达S-100和波形蛋白。电镜观察可见细胞形态多样,有些细胞含成束的张力丝,有些相邻细胞间有桥粒结构,部分细胞内可见病毒颗粒。细胞Ia表达呈强阳性,细胞倍增时间为24h。染色体为异倍体(50~78条),结果表明,本实验所建成的MFLC是以上皮细胞为主的已转化的胎肝混合细胞系。 展开更多
关键词 上皮细胞 细胞学 小鼠 基质细胞 肝脏 mflc
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Synthesis of Nonlinear Control of Switching Topologies of Buck-Boost Converter Using Fuzzy Logic on Field Programmable Gate Array (FPGA) 被引量:1
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作者 Johnson A. Asumadu Vaidhyanathan Jagannathan Arkhom Chachavalnanont 《Journal of Intelligent Learning Systems and Applications》 2010年第1期36-42,共7页
An intelligent fuzzy logic inference pipeline for the control of a dc-dc buck-boost converter was designed and built using a semi-custom VLSI chip. The fuzzy linguistics describing the switching topologies of the conv... An intelligent fuzzy logic inference pipeline for the control of a dc-dc buck-boost converter was designed and built using a semi-custom VLSI chip. The fuzzy linguistics describing the switching topologies of the converter was mapped into a look-up table that was synthesized into a set of Boolean equations. A VLSI chip–a field programmable gate array (FPGA) was used to implement the Boolean equations. Features include the size of RAM chip independent of number of rules in the knowledge base, on-chip fuzzification and defuzzification, faster response with speeds over giga fuzzy logic inferences per sec (FLIPS), and an inexpensive VLSI chip. The key application areas are: 1) on-chip integrated controllers;and 2) on-chip co-integration for entire system of sensors, circuits, controllers, and detectors for building complete instrument systems. 展开更多
关键词 Multi-Fuzzy Logic Controller (mflc) Field PROGRAMMABLE Gate Array (FPGA) BUCK-BOOST Converter BOOLEAN Look-Up TABLE CO-INTEGRATION
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