In insects,the juvenile hormone(JH)and 20-hydroxyecdysone(20E)pathways jointly regulate fecundity,but only methyl farnesoate(MF)and ponasterone A exist in mites.Comparative transcriptomic analysis in Panonychus citri ...In insects,the juvenile hormone(JH)and 20-hydroxyecdysone(20E)pathways jointly regulate fecundity,but only methyl farnesoate(MF)and ponasterone A exist in mites.Comparative transcriptomic analysis in Panonychus citri showed that E75B was significantly downregulated when exposed to lufenuron.Knockdown of E75B significantly affects the expression of vitellogenin(Vg),Fushi tarazu factor 1(Ftz-f1)and juvenile hormone acid O-methyltransferase(JHAMT),reducing fecundity in mites.The knockdown of Ftz-f1 produced a more significant effect than the knockdown of E75B,indicating that the ponasterone A pathway positively regulates fecundity in P.citri.After the knockdown of JHAMT,the expression levels of both Vg and Ftz-f1 and fecundity were significantly increased,along with the inhibition of Kr-h1,suggesting that JHAMT was negatively correlated with fecundity in the regulatory network.Knockdown of Kr-h1 inhibited the expression of Vg and Ftz-f1 and fecundity,and whether the drop in fecundity is caused by Kr-h1 or Ftz-f1 is unclear.Subsequent feeding with MF induced Kr-h1 and Vg expression,whereas no significant effects were observed for JHAMT and Ftz-f1.Therefore,the MF pathway stimulates fecundity independently.RNA interference(RNAi)showed that JHAMT and Ftz-f1 inhibited each other,resulting in opposite effects of MF and ponasterone A pathways on steady-state fecundity when either factor changed.Meanwhile,JHAMT knockdown led to increased fecundity,indicating that the stimulating effect of the ponasterone A pathway was greater than the inhibiting effect of the MF pathway,and demonstrating the dominant role of the ponasterone A pathway.Therefore,the interaction between JHAMT and Ftz-f1 may be closely associated with the maintenance of MF-ponasterone A regulatory network homeostasis and is involved in the reduction of fecundity in P.citri induced by exposure to lufenuron.展开更多
基金supported financially by the Fundamental Research Funds for the Central Universities(SWU-XDPY22001)of China“Pioneer"and“Leading Goose"R&D Program of Zhejiang Province(2023C04014)the China Agriculture Research System of MOF and MARA.
文摘In insects,the juvenile hormone(JH)and 20-hydroxyecdysone(20E)pathways jointly regulate fecundity,but only methyl farnesoate(MF)and ponasterone A exist in mites.Comparative transcriptomic analysis in Panonychus citri showed that E75B was significantly downregulated when exposed to lufenuron.Knockdown of E75B significantly affects the expression of vitellogenin(Vg),Fushi tarazu factor 1(Ftz-f1)and juvenile hormone acid O-methyltransferase(JHAMT),reducing fecundity in mites.The knockdown of Ftz-f1 produced a more significant effect than the knockdown of E75B,indicating that the ponasterone A pathway positively regulates fecundity in P.citri.After the knockdown of JHAMT,the expression levels of both Vg and Ftz-f1 and fecundity were significantly increased,along with the inhibition of Kr-h1,suggesting that JHAMT was negatively correlated with fecundity in the regulatory network.Knockdown of Kr-h1 inhibited the expression of Vg and Ftz-f1 and fecundity,and whether the drop in fecundity is caused by Kr-h1 or Ftz-f1 is unclear.Subsequent feeding with MF induced Kr-h1 and Vg expression,whereas no significant effects were observed for JHAMT and Ftz-f1.Therefore,the MF pathway stimulates fecundity independently.RNA interference(RNAi)showed that JHAMT and Ftz-f1 inhibited each other,resulting in opposite effects of MF and ponasterone A pathways on steady-state fecundity when either factor changed.Meanwhile,JHAMT knockdown led to increased fecundity,indicating that the stimulating effect of the ponasterone A pathway was greater than the inhibiting effect of the MF pathway,and demonstrating the dominant role of the ponasterone A pathway.Therefore,the interaction between JHAMT and Ftz-f1 may be closely associated with the maintenance of MF-ponasterone A regulatory network homeostasis and is involved in the reduction of fecundity in P.citri induced by exposure to lufenuron.
