采用qRT-PCR等技术探究了Mel1c在鹅不同器官组织(心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及F1、F2、F3、F4、F5卵泡)中的表达分布。结果表明,Mel1c m RNA在鹅的心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及...采用qRT-PCR等技术探究了Mel1c在鹅不同器官组织(心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及F1、F2、F3、F4、F5卵泡)中的表达分布。结果表明,Mel1c m RNA在鹅的心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及各级卵泡中均有表达;其表达量在各组织中存在差异,且以胰脏、脾脏、肾脏、卵巢中表达量较高,肺脏次之,心脏、胸肌、大脑水平相当,肝脏中最少。而在卵泡组织中,F4级卵泡中最高,其次为F5级卵泡,F1级卵泡中表达量最少。Mel1c在鹅各组织中普遍表达分布,进一步说明了褪黑素在机体中有着广泛的生物学作用,特别是对卵泡发育的影响。展开更多
为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆...为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆的序列与 Gen Bank中的序列一致。利用线性化的质粒模板反转出 c RNA探针 。展开更多
Translational regulation,especially tissue-or cell type-specific gene regulation,plays essential roles in plant growth and development.Thermo-sensitive genic male sterile(TGMS)lines have been widely used for hybrid br...Translational regulation,especially tissue-or cell type-specific gene regulation,plays essential roles in plant growth and development.Thermo-sensitive genic male sterile(TGMS)lines have been widely used for hybrid breeding in rice(Oryza sativa).However,little is known about translational regulation during reproductive stage in TGMS rice.Here,we use translating ribosome affinity purification(TRAP)combined with RNA sequencing to investigate the reproductive tissue-specific translatome of TGMS rice expressing FLAG-tagged ribosomal protein L18(RPL18)from the germline-specific promoter MEIOSIS ARRESTED AT LEPTOTENE1(MEL1).Differentially expressed genes at the transcriptional and translational levels are enriched in pollen and anther-related formation and development processes.These contain a number of genes reported to be involved in tapetum programmed cell death(PCD)and lipid metabolism during pollen development and anther dehiscence in rice,including several encoding transcription factors and key enzymes,as well as several long non-coding RNAs(lnc RNAs)that potentially affect tapetum and pollenrelated genes in male sterility.This study represents the comprehensive reproductive tissue-specific characterization of the translatome in TGMS rice.These results contribute to our understanding of the molecular basis of sterility in TGMS rice and will facilitate further genetic manipulation of TGMS rice in two-line breeding systems.展开更多
文摘采用qRT-PCR等技术探究了Mel1c在鹅不同器官组织(心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及F1、F2、F3、F4、F5卵泡)中的表达分布。结果表明,Mel1c m RNA在鹅的心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及各级卵泡中均有表达;其表达量在各组织中存在差异,且以胰脏、脾脏、肾脏、卵巢中表达量较高,肺脏次之,心脏、胸肌、大脑水平相当,肝脏中最少。而在卵泡组织中,F4级卵泡中最高,其次为F5级卵泡,F1级卵泡中表达量最少。Mel1c在鹅各组织中普遍表达分布,进一步说明了褪黑素在机体中有着广泛的生物学作用,特别是对卵泡发育的影响。
文摘为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆的序列与 Gen Bank中的序列一致。利用线性化的质粒模板反转出 c RNA探针 。
基金supported by grants from the National Key Research and Development Program of China (2020YFA0509900)the National Natural Science Foundation of China (31788103, 32171284, 31991184 and 31701096)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences (XDA24010302)the State Key Laboratory of Plant Genomics, China
文摘Translational regulation,especially tissue-or cell type-specific gene regulation,plays essential roles in plant growth and development.Thermo-sensitive genic male sterile(TGMS)lines have been widely used for hybrid breeding in rice(Oryza sativa).However,little is known about translational regulation during reproductive stage in TGMS rice.Here,we use translating ribosome affinity purification(TRAP)combined with RNA sequencing to investigate the reproductive tissue-specific translatome of TGMS rice expressing FLAG-tagged ribosomal protein L18(RPL18)from the germline-specific promoter MEIOSIS ARRESTED AT LEPTOTENE1(MEL1).Differentially expressed genes at the transcriptional and translational levels are enriched in pollen and anther-related formation and development processes.These contain a number of genes reported to be involved in tapetum programmed cell death(PCD)and lipid metabolism during pollen development and anther dehiscence in rice,including several encoding transcription factors and key enzymes,as well as several long non-coding RNAs(lnc RNAs)that potentially affect tapetum and pollenrelated genes in male sterility.This study represents the comprehensive reproductive tissue-specific characterization of the translatome in TGMS rice.These results contribute to our understanding of the molecular basis of sterility in TGMS rice and will facilitate further genetic manipulation of TGMS rice in two-line breeding systems.
