supported by grants from the National Basic Research Program of China （2014CB138404）;the China National Science Fund for Innovative Research Groups of Biological Control （31321063）;the National Basic Research Prog...supported by grants from the National Basic Research Program of China （2014CB138404）;the China National Science Fund for Innovative Research Groups of Biological Control （31321063）;the National Basic Research Program of China （973 Program, 2009CB119203）;the National Natural Science Foundation for Young Scientists in China （31101674）展开更多
Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity orB. tabaci and their associated endosymbion...Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity orB. tabaci and their associated endosymbionts in Africa. More than 28 species have been described in the complex ofB. tabaci cryptic species; among them, 2 are invasive pests worldwide： MED and MEAM1. In order to assess the species diversity orB. tabaci in vegetable crops in Senegal, several samplings in different localities, hosts and seasons were collected and analyzed with nuclear （microsatellite） and mitochondrial （COI） markers. The bacterial endosymbiont community was also studied for each sample. Two species were detected： MED Q1 and MEAM1 B. Patterns of MED Q1 （dominance on most of the samples and sites, highest nuclear and mitochondrial diversity and broader secondary endosymbiont community： Hamiltonella, Cardinium, Wolbachia and Rickettsia）, point toward a predominant resident begomovirus vector group for MED Q 1 on market gardening crops. Furthermore, the lower prevalence of the second species MEAM1 B, its lower nuclear and mitochondrial diversity and a narrower secondary endosymbiont community （Hamiltonella/Rickettsia）, indicate that this genetic group is exotic and results from a recent invasion in this area.展开更多
The whitefly Bemisia tabaci （Gennadius） is one of the most important pests causing economic losses in a variety of cropping systems around the world. This species was recently found in a coastal region of Colombia a...The whitefly Bemisia tabaci （Gennadius） is one of the most important pests causing economic losses in a variety of cropping systems around the world. This species was recently found in a coastal region of Colombia and has now spread inland. To investigate this invasive process, the genetic structure of B. tabaci was examined in 8 sampling locations from 2 infested regions （coastal, inland） using 9 microsatellite markers and the mitochondrial COI gene. The mitochondrial analysis indicated that only the invasive species of the B. tabaci complex Middle East-Asia Minor 1 （MEAM 1 known previously as biotype B） was present. The microsatellite data pointed to genetic differences among the regions and no isolation by distance within regions. The coastal region in the Caribbean appears to have been the initial point of invasion, while the inland region in the Southwest showed genetic variation among populations most likely reflecting founder events and ongoing changes associated with climatic and topographical heterogeneity. These findings have implications for tracking and managing B. tabaci.展开更多
文摘[目的]植物体内蛋白酶抑制剂(Protease inhibitors,PIs)主要参与防御病原物和植食性害虫为害以及应对非生物胁迫。但关于辣椒受MEAM1烟粉虱为害后,PIs的诱导和功能研究鲜见,相关研究有待深入。[方法]本研究拟通过对辣椒PIs的cDNA序列进行聚类分析,并运用qRT-PCR比较MEAM1烟粉虱(Middle East-Asia Minor 1)取食辣椒与对照未受害辣椒叶片PIs生物合成基因表达量差异,探究PIs在辣椒防御MEAM1为害过程中发挥作用。[结果]研究结果显示,辣椒体内的PI-Ⅱ类型基因进化保守,MEAM1烟粉虱取食可以诱导辣椒PIs生物合成相关基因表达显著上调。[结论]推测辣椒PIs在辣椒防御MEAM1烟粉虱为害过程中发挥重要作用。
基金supported by grants from the National Basic Research Program of China (2014CB138404)the China National Science Fund for Innovative Research Groups of Biological Control (31321063)+1 种基金the National Basic Research Program of China (973 Program, 2009CB119203)the National Natural Science Foundation for Young Scientists in China (31101674)
文摘supported by grants from the National Basic Research Program of China （2014CB138404）;the China National Science Fund for Innovative Research Groups of Biological Control （31321063）;the National Basic Research Program of China （973 Program, 2009CB119203）;the National Natural Science Foundation for Young Scientists in China （31101674）
文摘Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity orB. tabaci and their associated endosymbionts in Africa. More than 28 species have been described in the complex ofB. tabaci cryptic species; among them, 2 are invasive pests worldwide： MED and MEAM1. In order to assess the species diversity orB. tabaci in vegetable crops in Senegal, several samplings in different localities, hosts and seasons were collected and analyzed with nuclear （microsatellite） and mitochondrial （COI） markers. The bacterial endosymbiont community was also studied for each sample. Two species were detected： MED Q1 and MEAM1 B. Patterns of MED Q1 （dominance on most of the samples and sites, highest nuclear and mitochondrial diversity and broader secondary endosymbiont community： Hamiltonella, Cardinium, Wolbachia and Rickettsia）, point toward a predominant resident begomovirus vector group for MED Q 1 on market gardening crops. Furthermore, the lower prevalence of the second species MEAM1 B, its lower nuclear and mitochondrial diversity and a narrower secondary endosymbiont community （Hamiltonella/Rickettsia）, indicate that this genetic group is exotic and results from a recent invasion in this area.
文摘The whitefly Bemisia tabaci （Gennadius） is one of the most important pests causing economic losses in a variety of cropping systems around the world. This species was recently found in a coastal region of Colombia and has now spread inland. To investigate this invasive process, the genetic structure of B. tabaci was examined in 8 sampling locations from 2 infested regions （coastal, inland） using 9 microsatellite markers and the mitochondrial COI gene. The mitochondrial analysis indicated that only the invasive species of the B. tabaci complex Middle East-Asia Minor 1 （MEAM 1 known previously as biotype B） was present. The microsatellite data pointed to genetic differences among the regions and no isolation by distance within regions. The coastal region in the Caribbean appears to have been the initial point of invasion, while the inland region in the Southwest showed genetic variation among populations most likely reflecting founder events and ongoing changes associated with climatic and topographical heterogeneity. These findings have implications for tracking and managing B. tabaci.
