Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two c...Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.展开更多
RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMO...RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.展开更多
基金supported by the National Natural Science Foundation of China(32470391,32401801,and 31870322)the Open Competitive Project of the Wuhan East Lake high-tech Zone(2023KJB220)the Key Research and Development Program of Hubei Province(2025BBA002).
文摘Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.
基金supported by National Natural Science Foundation of China(31770782)the Ministry of Science and Technology of China(2016YFA0503200)+1 种基金the Shenzhen Science and Technology Program(JCYJ20200109110403829 and KQTD20190929173906742)Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes(2019KSYS006)to J.D.
文摘RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.
