Disruption of the MEKK1-MKK1/MKK2-MPK4 kinase cascade leads to activation of immunity mediated by the nucleotide-binding leucine-rich repeat(NLR)immune receptor SUMM2,which monitors the phosphorylation status of CRCK3...Disruption of the MEKK1-MKK1/MKK2-MPK4 kinase cascade leads to activation of immunity mediated by the nucleotide-binding leucine-rich repeat(NLR)immune receptor SUMM2,which monitors the phosphorylation status of CRCK3.Here we report that two receptor-like kinases(RLKs),MDS1,and MDS2,function redundantly to promote SUMM2-mediated immunity.Activation of SUMM2-mediated immunity is dependent on MDS1,and to a less extent on MDS2.MDS1 associates with CRCK3 in planta and can phosphorylate CRCK3 in vitro,suggesting that it may target CRCK3 to positively regulate SUMM2-mediated signaling.Our finding highlights a new defense mechanism where RLKs promote NLR-mediated immunity.展开更多
Objective To investigate expression of Evi 1 and MDS1 Evi 1 genes in myelodysplastic syndromes (MDS) and post MDS acute myeloid leukemia (post MDS AML), and its role in pathogenesis or progression of MDS and pos...Objective To investigate expression of Evi 1 and MDS1 Evi 1 genes in myelodysplastic syndromes (MDS) and post MDS acute myeloid leukemia (post MDS AML), and its role in pathogenesis or progression of MDS and post MDS AML Methods Expression of Evi 1 and MDS1 Evi 1 genes was examined in 31 MDS, 11 post MDS AML, and 34 de novo AML patients by a semi quantitative reverse transcription polymerase chain reaction (RT PCR) Results Evi 1 expression was not detected in bone marrow samples of 8 normal controls, but low MDS1 Evi 1 expression levels (MDS1 Evi 1/GAPDH<0 1) were detected in 3 of the 8 controls Evi 1 RNA was expressed in 1 of 8 RA, 8 of 13 RAEB and 6 of 9 RAEB T patients, and the percentage of Evi 1 expression in RAEB(T) patients was higher than that in RA (P<0 05) MDS1 Evi 1 expression was detected in 5 of 8 RA, 9 of 13 RAEB and 5 of 9 RAEB T patients, and MDS1 Evi 1 expression levels (MDS1 Evi 1/GAPDH>0 1) were markedly higher than those in the controls Evi 1 expression was gradually increased in 4 of 5 RAEB T patients with transformation from MDS to AML The percentages of Evi 1 and MDS1 Evi 1 expression in post MDS AML patients were significantly (P<0 01 and P<0 05 respectively) higher than those in de novo AML The colonies of hematopoietic progenitor cells were decreased in Evi 1 and MDS1 Evi 1 positive MDS patients as compared with those in Evi 1 and MDS1 Evi 1 negative patients Conclusion Abnormal expression of the Evi 1 gene and overexpression of MDS1 Evi 1 gene may play a role in the pathogenesis or progression of MDS and post MDS AML展开更多
以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PG...以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PGDH1还有另外3个同源基因;功能域分析表明Md6PGDH蛋白含有两个保守的绑定域;亚细胞预测表明Md6PGDH定位存在差异。分析Md6PGDH1启动子发现存在多个响应非生物胁迫的顺式作用元件。定量分析显示,Md6PGDH1在苹果的不同组织中都有表达,且受非生物胁迫诱导。原核诱导Md6PGDH1蛋白并进行蛋白酶活的测定,为后续蛋白功能鉴定奠定了基础。Md6PGDH1在苹果愈伤组织中过量表达,提高其抗盐胁迫的能力。展开更多
Objective:To investigate the effects of polysacharide MD 1 on eliminating free radical O -· 2,·OH and anti peroxidation on human erythrocyte membrane. Methods:testing the effect of eliminating O -· 2?&#...Objective:To investigate the effects of polysacharide MD 1 on eliminating free radical O -· 2,·OH and anti peroxidation on human erythrocyte membrane. Methods:testing the effect of eliminating O -· 2?·OH of MD 1 at different concentrations in vitro;testing the influence of MD 1 on peroxidation of free radical of human erythrocyte membrane by fluorescence. Results:Polyssacharide MD 1 could eliminate O -· 2 and·OH(<250 mg/L).compared with the control group( P <0.01). The result also showed that MD 1 could inhibit lipid peroxidation of human erythrocyte membrane,compared with the control group( P <0.01).展开更多
文摘Disruption of the MEKK1-MKK1/MKK2-MPK4 kinase cascade leads to activation of immunity mediated by the nucleotide-binding leucine-rich repeat(NLR)immune receptor SUMM2,which monitors the phosphorylation status of CRCK3.Here we report that two receptor-like kinases(RLKs),MDS1,and MDS2,function redundantly to promote SUMM2-mediated immunity.Activation of SUMM2-mediated immunity is dependent on MDS1,and to a less extent on MDS2.MDS1 associates with CRCK3 in planta and can phosphorylate CRCK3 in vitro,suggesting that it may target CRCK3 to positively regulate SUMM2-mediated signaling.Our finding highlights a new defense mechanism where RLKs promote NLR-mediated immunity.
文摘Objective To investigate expression of Evi 1 and MDS1 Evi 1 genes in myelodysplastic syndromes (MDS) and post MDS acute myeloid leukemia (post MDS AML), and its role in pathogenesis or progression of MDS and post MDS AML Methods Expression of Evi 1 and MDS1 Evi 1 genes was examined in 31 MDS, 11 post MDS AML, and 34 de novo AML patients by a semi quantitative reverse transcription polymerase chain reaction (RT PCR) Results Evi 1 expression was not detected in bone marrow samples of 8 normal controls, but low MDS1 Evi 1 expression levels (MDS1 Evi 1/GAPDH<0 1) were detected in 3 of the 8 controls Evi 1 RNA was expressed in 1 of 8 RA, 8 of 13 RAEB and 6 of 9 RAEB T patients, and the percentage of Evi 1 expression in RAEB(T) patients was higher than that in RA (P<0 05) MDS1 Evi 1 expression was detected in 5 of 8 RA, 9 of 13 RAEB and 5 of 9 RAEB T patients, and MDS1 Evi 1 expression levels (MDS1 Evi 1/GAPDH>0 1) were markedly higher than those in the controls Evi 1 expression was gradually increased in 4 of 5 RAEB T patients with transformation from MDS to AML The percentages of Evi 1 and MDS1 Evi 1 expression in post MDS AML patients were significantly (P<0 01 and P<0 05 respectively) higher than those in de novo AML The colonies of hematopoietic progenitor cells were decreased in Evi 1 and MDS1 Evi 1 positive MDS patients as compared with those in Evi 1 and MDS1 Evi 1 negative patients Conclusion Abnormal expression of the Evi 1 gene and overexpression of MDS1 Evi 1 gene may play a role in the pathogenesis or progression of MDS and post MDS AML
文摘以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PGDH1还有另外3个同源基因;功能域分析表明Md6PGDH蛋白含有两个保守的绑定域;亚细胞预测表明Md6PGDH定位存在差异。分析Md6PGDH1启动子发现存在多个响应非生物胁迫的顺式作用元件。定量分析显示,Md6PGDH1在苹果的不同组织中都有表达,且受非生物胁迫诱导。原核诱导Md6PGDH1蛋白并进行蛋白酶活的测定,为后续蛋白功能鉴定奠定了基础。Md6PGDH1在苹果愈伤组织中过量表达,提高其抗盐胁迫的能力。
文摘Objective:To investigate the effects of polysacharide MD 1 on eliminating free radical O -· 2,·OH and anti peroxidation on human erythrocyte membrane. Methods:testing the effect of eliminating O -· 2?·OH of MD 1 at different concentrations in vitro;testing the influence of MD 1 on peroxidation of free radical of human erythrocyte membrane by fluorescence. Results:Polyssacharide MD 1 could eliminate O -· 2 and·OH(<250 mg/L).compared with the control group( P <0.01). The result also showed that MD 1 could inhibit lipid peroxidation of human erythrocyte membrane,compared with the control group( P <0.01).
