A major challenge in the treatment of Tuberculosis (TB) is emergence of Multi-Drug Resistant <em>Mycobacterium tuberculosis</em> (MDRTB) strains. Efflux genes have been established to be among factors for ...A major challenge in the treatment of Tuberculosis (TB) is emergence of Multi-Drug Resistant <em>Mycobacterium tuberculosis</em> (MDRTB) strains. Efflux genes have been established to be among factors for drug resistance in <em>Mycobacterium tuberculosis</em> (<em>M. tuberculosis</em>) pulmonary infections by conferring bacterial ability to pump-out drugs from intracellular compartment, making it impossible for drugs to attain intracellular concentration lethal to the organism. There is paucity of data on the role of efflux pump in MDRTB in Nigerian strains of<em> M. tuberculosis</em>. Hence, the aim of this study was to detect the carriage, distribution and frequency of efflux pump genes among MDRTB and non-MDRTB isolates from participants with pulmonary tuberculosis in Lagos, Nigeria. This study was carried out on <em>M. tuberculosis</em> isolated from 1020 participants suspected of pulmonary tuberculosis in Lagos State, Nigeria. A total of 78 <em>M. tuberculosis</em> isolates were obtained from the participants suspected of pulmonary tuberculosis. Forty Eight isolates were confirmed as MDRTB and 30 non-MDRTB. Efflux pump genes were investigated in the isolates using the conventional polymerase chain reaction. Statistical analysis was carried out using the Statistical Package for Social Science (SPSS version 20) to compare the efflux pump gene results between MDRTB and non- MDRTB isolates. Different efflux genes types and frequency were detected in MDRTB and non-MDRTB isolates. Carriage of 2 or more alleles of efflux gene types Rv2486c (efpA), Rv2459c (jefA), Rv1877, Rv1002c, Rv0342, Rv2686c and drrC associated with MDR were detected. Additionally, the frequency of efflux genes alleles in MDRTB was significantly different from those in non- MDRTB isolates.展开更多
文摘A major challenge in the treatment of Tuberculosis (TB) is emergence of Multi-Drug Resistant <em>Mycobacterium tuberculosis</em> (MDRTB) strains. Efflux genes have been established to be among factors for drug resistance in <em>Mycobacterium tuberculosis</em> (<em>M. tuberculosis</em>) pulmonary infections by conferring bacterial ability to pump-out drugs from intracellular compartment, making it impossible for drugs to attain intracellular concentration lethal to the organism. There is paucity of data on the role of efflux pump in MDRTB in Nigerian strains of<em> M. tuberculosis</em>. Hence, the aim of this study was to detect the carriage, distribution and frequency of efflux pump genes among MDRTB and non-MDRTB isolates from participants with pulmonary tuberculosis in Lagos, Nigeria. This study was carried out on <em>M. tuberculosis</em> isolated from 1020 participants suspected of pulmonary tuberculosis in Lagos State, Nigeria. A total of 78 <em>M. tuberculosis</em> isolates were obtained from the participants suspected of pulmonary tuberculosis. Forty Eight isolates were confirmed as MDRTB and 30 non-MDRTB. Efflux pump genes were investigated in the isolates using the conventional polymerase chain reaction. Statistical analysis was carried out using the Statistical Package for Social Science (SPSS version 20) to compare the efflux pump gene results between MDRTB and non- MDRTB isolates. Different efflux genes types and frequency were detected in MDRTB and non-MDRTB isolates. Carriage of 2 or more alleles of efflux gene types Rv2486c (efpA), Rv2459c (jefA), Rv1877, Rv1002c, Rv0342, Rv2686c and drrC associated with MDR were detected. Additionally, the frequency of efflux genes alleles in MDRTB was significantly different from those in non- MDRTB isolates.
