BACKGROUND Well-differentiated small bowel mesenteric liposarcoma(LPS)is rare,with high malignancy,poor prognosis,and high preponderance to local recurrence.CASE SUMMARY Here we described a 71-year-old male,who compla...BACKGROUND Well-differentiated small bowel mesenteric liposarcoma(LPS)is rare,with high malignancy,poor prognosis,and high preponderance to local recurrence.CASE SUMMARY Here we described a 71-year-old male,who complains of persistent abdominal distension for a month.The clinical manifestation is a huge abdominal mass occupying almost the entire abdomen.Physical examination indicated palpable massive mass in the abdomen,hard texture,indefinable boundary,poor mobility.The abdominal enhanced computed tomography at another hospital scan showed multiple abdominal masses originating from the small bowel mesentery.Abdominal and pelvic magnetic resonance imaging at our hospital showed multiple masses in the abdominal and pelvic cavities,indicating that the tumor originated from the mesentery or peritoneum.Results of exploratory laparotomy indicated that the tremendous mass primarily results from the mesentery of the small intestine,occupying the entire abdominal cavity in a polymorphic and lobulated shape.The patient underwent complete surgical resection of the tumor,and the weight of the tumor was approximately 11 kg.The histopathological examination of the resected specimens confirmed the diagnosis of well-differentiated LPS of the small bowel mesentery.CONCLUSION Completed surgical resection was cornerstone,and histopathological and molecular confirmations were crucial.The necessity of adjuvant therapy should be phrased as a potential consideration to improve patient’s survival time.展开更多
Oroxylin A(OA),a natural compound extracted from Scutellaria baicalensis,demonstrates preventive potential against ultraviolet B(UVB)-induced non-melanoma skin cancer(NMSC),the most prevalent cancer worldwide with inc...Oroxylin A(OA),a natural compound extracted from Scutellaria baicalensis,demonstrates preventive potential against ultraviolet B(UVB)-induced non-melanoma skin cancer(NMSC),the most prevalent cancer worldwide with increasing incidence.Utilizing SKH-1 hairless mice exposed to UVB,this study showed that OA delayed NMSC onset and alleviated acute skin damage.Mechanistic investigations revealed its dual action:inhibiting inflammation and enhancing nucleotide excision repair(NER)by stabilizing XPA,a crucial deoxyribonucleic acid(DNA)repair protein.This stabilization occurred through OA's interaction with glucose-regulated protein 94(GRP94),which disrupted murine double minute 2(MDM2)-mediated XPA ubiquitination and proteasomal degradation.By maintaining XPA levels,OA expedited photoproduct clearance and diminished genomic instability,ultimately impeding NMSC development.These findings suggest OA as a promising chemopreventive agent targeting the GRP94/MDM2-XPA axis to counteract UVB-induced carcinogenesis.展开更多
Objective:Identifying biomarkers that predict the efficacy and prognosis of chemoradiotherapy is important for individualized clinical treatment.We previously reported that high murine double minute 1(MDM1)expression ...Objective:Identifying biomarkers that predict the efficacy and prognosis of chemoradiotherapy is important for individualized clinical treatment.We previously reported that high murine double minute 1(MDM1)expression in patients with rectal cancer is linked to a favorable chemoradiation response.In this study the role of MDM1 in the chemoradiotherapy response in colorectal cancer(CRC)patients was evaluated.Methods:Colony formation and cell proliferation assays as well as xenograft models were used to determine if MDM1 expression affects the sensitivity of CRC cells to chemoradiation.RNA sequencing revealed that MDM1 regulates tumor protein 53(TP53)expression and apoptosis.A series of molecular biology experiments were performed to determine how MDM1 affects p53 expression.The effects of inhibitors targeting apoptosis on MDM1 knockout cells were evaluated.Results:Gene expression profiling revealed that MDM1 is a potential chemoradiotherapy sensitivity marker.The sensitivity of CRC cells to chemoradiation treatment decreased after MDM1 knockout and increased after MDM1 overexpression.MDM1 affected p53 expression,thereby regulating apoptosis.MDM1 overexpression limited YBX1 binding to TP53 promoter,regulated TP53 expression,and rendered CRC cells more sensitive to chemoradiation.In CRC cells with low MDM1 expression,a combination of apoptosis-inducing inhibitors and chemoradiation treatment restored sensitivity to cancer therapy.Conclusions:The current study showed that MDM1 expression influences the sensitivity of CRC cells to chemoradiation by influencing p53 and apoptosis pathways,which is the basis for the underlying molecular mechanism,and serves as a possible predictive marker for chemoradiotherapy prognosis.展开更多
Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in man...Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
文摘BACKGROUND Well-differentiated small bowel mesenteric liposarcoma(LPS)is rare,with high malignancy,poor prognosis,and high preponderance to local recurrence.CASE SUMMARY Here we described a 71-year-old male,who complains of persistent abdominal distension for a month.The clinical manifestation is a huge abdominal mass occupying almost the entire abdomen.Physical examination indicated palpable massive mass in the abdomen,hard texture,indefinable boundary,poor mobility.The abdominal enhanced computed tomography at another hospital scan showed multiple abdominal masses originating from the small bowel mesentery.Abdominal and pelvic magnetic resonance imaging at our hospital showed multiple masses in the abdominal and pelvic cavities,indicating that the tumor originated from the mesentery or peritoneum.Results of exploratory laparotomy indicated that the tremendous mass primarily results from the mesentery of the small intestine,occupying the entire abdominal cavity in a polymorphic and lobulated shape.The patient underwent complete surgical resection of the tumor,and the weight of the tumor was approximately 11 kg.The histopathological examination of the resected specimens confirmed the diagnosis of well-differentiated LPS of the small bowel mesentery.CONCLUSION Completed surgical resection was cornerstone,and histopathological and molecular confirmations were crucial.The necessity of adjuvant therapy should be phrased as a potential consideration to improve patient’s survival time.
基金supported by the National Natural Science Foundation of China(No.81974425)the Natural Science Foundation of Jiangsu Province(Nos.BK20211578 and BK20210419)+1 种基金the China Postdoctoral Science Foundation Grant(No.2021M693513)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22-0794)。
文摘Oroxylin A(OA),a natural compound extracted from Scutellaria baicalensis,demonstrates preventive potential against ultraviolet B(UVB)-induced non-melanoma skin cancer(NMSC),the most prevalent cancer worldwide with increasing incidence.Utilizing SKH-1 hairless mice exposed to UVB,this study showed that OA delayed NMSC onset and alleviated acute skin damage.Mechanistic investigations revealed its dual action:inhibiting inflammation and enhancing nucleotide excision repair(NER)by stabilizing XPA,a crucial deoxyribonucleic acid(DNA)repair protein.This stabilization occurred through OA's interaction with glucose-regulated protein 94(GRP94),which disrupted murine double minute 2(MDM2)-mediated XPA ubiquitination and proteasomal degradation.By maintaining XPA levels,OA expedited photoproduct clearance and diminished genomic instability,ultimately impeding NMSC development.These findings suggest OA as a promising chemopreventive agent targeting the GRP94/MDM2-XPA axis to counteract UVB-induced carcinogenesis.
基金supported by grants from the National Natural Science Foundation(Grant No.81972859 to W.T.)Beijing Municipal Science&Technology Commission Grant(Grant No.D0905001040531 to D.L.)State Key Laboratory of Molecular Oncology Grant(Grant No.SKLMO-KF2023-03 to D.L.).
文摘Objective:Identifying biomarkers that predict the efficacy and prognosis of chemoradiotherapy is important for individualized clinical treatment.We previously reported that high murine double minute 1(MDM1)expression in patients with rectal cancer is linked to a favorable chemoradiation response.In this study the role of MDM1 in the chemoradiotherapy response in colorectal cancer(CRC)patients was evaluated.Methods:Colony formation and cell proliferation assays as well as xenograft models were used to determine if MDM1 expression affects the sensitivity of CRC cells to chemoradiation.RNA sequencing revealed that MDM1 regulates tumor protein 53(TP53)expression and apoptosis.A series of molecular biology experiments were performed to determine how MDM1 affects p53 expression.The effects of inhibitors targeting apoptosis on MDM1 knockout cells were evaluated.Results:Gene expression profiling revealed that MDM1 is a potential chemoradiotherapy sensitivity marker.The sensitivity of CRC cells to chemoradiation treatment decreased after MDM1 knockout and increased after MDM1 overexpression.MDM1 affected p53 expression,thereby regulating apoptosis.MDM1 overexpression limited YBX1 binding to TP53 promoter,regulated TP53 expression,and rendered CRC cells more sensitive to chemoradiation.In CRC cells with low MDM1 expression,a combination of apoptosis-inducing inhibitors and chemoradiation treatment restored sensitivity to cancer therapy.Conclusions:The current study showed that MDM1 expression influences the sensitivity of CRC cells to chemoradiation by influencing p53 and apoptosis pathways,which is the basis for the underlying molecular mechanism,and serves as a possible predictive marker for chemoradiotherapy prognosis.
文摘Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
