Objectives:Mitochondrial Ca^(2+)uniporter(MCU)provides a Ca^(2+)influx pathway from the cytosol into the mitochondrial matrix and a moderate mitochondrial Ca^(2+)rise stimulates ATP production and cell growth.MCU is h...Objectives:Mitochondrial Ca^(2+)uniporter(MCU)provides a Ca^(2+)influx pathway from the cytosol into the mitochondrial matrix and a moderate mitochondrial Ca^(2+)rise stimulates ATP production and cell growth.MCU is highly expressed in various cancer cells including breast cancer cells,thereby increasing the capacity of mitochondrial Ca^(2+)uptake,ATP production,and cancer cell proliferation.The objective of this study was to examine MCU inhibition as an anti-cancer mechanism.Methods:The effects of MCU-i4,a newly developed MCU inhibitor,on cell viability,apoptosis,cytosolic Ca^(2+),mitochondrial Ca^(2+)and potential,glycolytic rate,generation of ATP,and reactive oxygen species,were examined in breast cancer BT474 cells.Results:MCU-i4 caused apoptotic cell death,and it decreased and increased,respectively,mitochondrial and cytosolic Ca^(2+)concentration.Inhibition of MCU by MCU-i4 revealed that cytosolic Ca^(2+)elevation resulted from endoplasmic reticulum(ER)Ca^(2+)release via inositol 1,4,5-trisphosphate receptors(IP3R)and ryanodine receptors(RYR).Unexpectedly,MCU-i4 enhanced glycolysis and ATP production;it also triggered a large production of reactive oxygen species(ROS)and mitochondrial membrane potential collapse.Conclusion:Cytotoxic mechanisms of MCU-i4 in cancer cells involved enhanced glycolysis and heightened formation of ATP and ROS.It is conventionally believed that cancer cell death could be caused by inhibition of glycolysis.Our observations suggest cancer cell death could also be induced by increased glycolytic metabolism.展开更多
为提升船舶喷推进器双微控制单元(Microcontroller Unit,MCU)冗余系统通信与故障检测功能,提高喷水推进器控制系统的可靠性和稳定性。在RS232串口心跳检测的基础上,通过自定义控制器局域网(Controller Area Network,CAN)通信协议,对现有...为提升船舶喷推进器双微控制单元(Microcontroller Unit,MCU)冗余系统通信与故障检测功能,提高喷水推进器控制系统的可靠性和稳定性。在RS232串口心跳检测的基础上,通过自定义控制器局域网(Controller Area Network,CAN)通信协议,对现有CAN通信网络进行功能复用,提出一种基于CAN总线的双MCU冗余上位机监控检测机制。实验测试结果表明,上位机监控检测方法克服了传统故障检测方法仅针对MCU故障而忽视其外围通信故障的缺点,避免了“双主机现象”,提高了冗余控制系统故障检测的速度与精准度,可以保障双MCU冗余系统平稳、可靠运行。研究成果可为喷水推进器双MCU冗余系统故障检测方式提供一定参考。展开更多
微控制器(Microcontroller Unit,MCU)芯片以其高性能、多功能、可编程等优点被广泛应用于各种信号处理和嵌入式系统。MCU芯片测试是保证产品可靠性的重要手段之一,由于市场上的MCU芯片愈发多样化,如何实现高性能和复杂功能的高效测试是...微控制器(Microcontroller Unit,MCU)芯片以其高性能、多功能、可编程等优点被广泛应用于各种信号处理和嵌入式系统。MCU芯片测试是保证产品可靠性的重要手段之一,由于市场上的MCU芯片愈发多样化,如何实现高性能和复杂功能的高效测试是MCU芯片测试的难点。论文针对一款32位高性能MCU芯片,基于自动测试设备(Automatic Test Equipment,ATE)开展了MCU芯片在线测试技术研究,详细说明了MCU电性能测试系统的硬件电路设计方法和软件程序实现流程,实现了批量MCU芯片的产线测试,保障了MCU芯片的可靠性。展开更多
大规模视频会议系统所面对的用户数量较多,且在运行时存在资源分配不均、负载过高等问题。为解决这一问题,需运用多点控制单元(Multipoint Control Unit,MCU)资源池实现多点控制,确保资源得到合理分配,提升视频会议系统性能。文章主要...大规模视频会议系统所面对的用户数量较多,且在运行时存在资源分配不均、负载过高等问题。为解决这一问题,需运用多点控制单元(Multipoint Control Unit,MCU)资源池实现多点控制,确保资源得到合理分配,提升视频会议系统性能。文章主要从组织、权限、服务区、网守号码、备份路由、组网规划等方面对MCU资源池进行设计,并提出资源池在大规模视频会议系统中的实际应用,以提升系统扩展性,确保MCU资源池满足大规模视频会议的需求。展开更多
基金China Medical University and China Medical University Hospital,Taiwan for providing fundings(CMU111-S-20,CMU112-S-59,DMR-112-067)ECS thanks An Nan Hospital for support(ANHRF112-04).
文摘Objectives:Mitochondrial Ca^(2+)uniporter(MCU)provides a Ca^(2+)influx pathway from the cytosol into the mitochondrial matrix and a moderate mitochondrial Ca^(2+)rise stimulates ATP production and cell growth.MCU is highly expressed in various cancer cells including breast cancer cells,thereby increasing the capacity of mitochondrial Ca^(2+)uptake,ATP production,and cancer cell proliferation.The objective of this study was to examine MCU inhibition as an anti-cancer mechanism.Methods:The effects of MCU-i4,a newly developed MCU inhibitor,on cell viability,apoptosis,cytosolic Ca^(2+),mitochondrial Ca^(2+)and potential,glycolytic rate,generation of ATP,and reactive oxygen species,were examined in breast cancer BT474 cells.Results:MCU-i4 caused apoptotic cell death,and it decreased and increased,respectively,mitochondrial and cytosolic Ca^(2+)concentration.Inhibition of MCU by MCU-i4 revealed that cytosolic Ca^(2+)elevation resulted from endoplasmic reticulum(ER)Ca^(2+)release via inositol 1,4,5-trisphosphate receptors(IP3R)and ryanodine receptors(RYR).Unexpectedly,MCU-i4 enhanced glycolysis and ATP production;it also triggered a large production of reactive oxygen species(ROS)and mitochondrial membrane potential collapse.Conclusion:Cytotoxic mechanisms of MCU-i4 in cancer cells involved enhanced glycolysis and heightened formation of ATP and ROS.It is conventionally believed that cancer cell death could be caused by inhibition of glycolysis.Our observations suggest cancer cell death could also be induced by increased glycolytic metabolism.
文摘为提升船舶喷推进器双微控制单元(Microcontroller Unit,MCU)冗余系统通信与故障检测功能,提高喷水推进器控制系统的可靠性和稳定性。在RS232串口心跳检测的基础上,通过自定义控制器局域网(Controller Area Network,CAN)通信协议,对现有CAN通信网络进行功能复用,提出一种基于CAN总线的双MCU冗余上位机监控检测机制。实验测试结果表明,上位机监控检测方法克服了传统故障检测方法仅针对MCU故障而忽视其外围通信故障的缺点,避免了“双主机现象”,提高了冗余控制系统故障检测的速度与精准度,可以保障双MCU冗余系统平稳、可靠运行。研究成果可为喷水推进器双MCU冗余系统故障检测方式提供一定参考。
文摘微控制器(Microcontroller Unit,MCU)芯片以其高性能、多功能、可编程等优点被广泛应用于各种信号处理和嵌入式系统。MCU芯片测试是保证产品可靠性的重要手段之一,由于市场上的MCU芯片愈发多样化,如何实现高性能和复杂功能的高效测试是MCU芯片测试的难点。论文针对一款32位高性能MCU芯片,基于自动测试设备(Automatic Test Equipment,ATE)开展了MCU芯片在线测试技术研究,详细说明了MCU电性能测试系统的硬件电路设计方法和软件程序实现流程,实现了批量MCU芯片的产线测试,保障了MCU芯片的可靠性。
文摘大规模视频会议系统所面对的用户数量较多,且在运行时存在资源分配不均、负载过高等问题。为解决这一问题,需运用多点控制单元(Multipoint Control Unit,MCU)资源池实现多点控制,确保资源得到合理分配,提升视频会议系统性能。文章主要从组织、权限、服务区、网守号码、备份路由、组网规划等方面对MCU资源池进行设计,并提出资源池在大规模视频会议系统中的实际应用,以提升系统扩展性,确保MCU资源池满足大规模视频会议的需求。
