目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭...目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭代优化,直至收敛。结果:采用此方法分辨所得光谱还原率高,定量结果的浓度值与真实值之间线性关系良好。结论:本方法用于药物色谱重叠峰分辨结果可靠。展开更多
The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the re...The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the reaction mechanism was proposed.展开更多
The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Ram...The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features.In this study,we combined ultrabroadband coherent anti-Stokes Raman scattering(CARS)microspectroscopy across the spectral range of 500−4000 cm^(−1)with multivariate curve resolution-alternating least-squares(MCRALS)to reveal hidden Raman bands in the fingerprint region.Applying this method to mouse brain tissue,we extracted information on cholesterol and collagen,leveraging their distinctive molecular signatures,as well as on key molecules such as lipids,proteins,water,and nucleic acids.Moreover,the simultaneous detection of second harmonic generation facilitated labelfree visualization of organelles,including arachnoid membrane and Rootletin filaments.展开更多
文摘目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭代优化,直至收敛。结果:采用此方法分辨所得光谱还原率高,定量结果的浓度值与真实值之间线性关系良好。结论:本方法用于药物色谱重叠峰分辨结果可靠。
基金The work was supported by the National Natural Science Foundation of China[Grant No.20075021]visiting grant of Reutlingen University of Applied Sciences in Germany.
文摘The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the reaction mechanism was proposed.
基金supported by the Japan Society for the Promotion of Science(JSPS)through a Grant-in-Aid for Scientific Research(A)(KAKENHI,grant number 21H04961)Japan Science and the Technology Agency(JST)through a Mirai Program grant(JPMJMI22G5)+1 种基金It also benefited from a Japan−France bilateral project awarded to H.K.and the support of the French government in the form of a grant managed by the National Research Agency under the Investments for the Future program(ANR-10-LABX-0074 Sigma-LIM)awarded to P.Lsupported by AMED under Grant Number JP21zf0127005.
文摘The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features.In this study,we combined ultrabroadband coherent anti-Stokes Raman scattering(CARS)microspectroscopy across the spectral range of 500−4000 cm^(−1)with multivariate curve resolution-alternating least-squares(MCRALS)to reveal hidden Raman bands in the fingerprint region.Applying this method to mouse brain tissue,we extracted information on cholesterol and collagen,leveraging their distinctive molecular signatures,as well as on key molecules such as lipids,proteins,water,and nucleic acids.Moreover,the simultaneous detection of second harmonic generation facilitated labelfree visualization of organelles,including arachnoid membrane and Rootletin filaments.
