Objective:To synthesize nanoformulated naringenin(NF-n)and evaluate its anti-angiogenic and anticancer activities.Methods:NF-n was synthesized using the solvent evaporation method and characterized by dynamic light sc...Objective:To synthesize nanoformulated naringenin(NF-n)and evaluate its anti-angiogenic and anticancer activities.Methods:NF-n was synthesized using the solvent evaporation method and characterized by dynamic light scattering,Fourier transform infrared spectroscopy,and scanning electron microscopy.Molecular docking studies were performed to assess NF-n’s binding affinity to vascular endothelial growth factor(VEGF).In vitro assays using HUVEC and MCF-7 cell lines were conducted to evaluate cytotoxicity and cell migration inhibition.The mRNA expression levels of angiogenesis-and inflammation-related markers(nestin,NRP-1,NRP-2,CD93,IL-1β,TNF-α,NF-κB,and Bcl-2)were quantified via RT-PCR.The anti-angiogenic effect of NF-n was further investigated using the chick chorioallantoic membrane assay.Results:Molecular docking revealed effective binding of naringenin to VEGF.NF-n demonstrated significantly reduced particle size and improved physicochemical properties.In in vitro studies,NF-n reduced cell viability and inhibited migration in both HUVEC and MCF-7 cells.RT-PCR analysis showed that NF-n significantly downregulated pro-angiogenic and inflammatory markers.Furthermore,NF-n significantly decreased blood vessel density,total branching points,and vessel length in heparin-induced chick chorioallantoic membrane.Conclusions:NF-n exhibits anti-angiogenic and anticancer properties,positioning it as a promising candidate for therapeutic application in cancer and other pathological conditions involving abnormal angiogenesis.Further preclinical studies are recommended to explore its translational potential.展开更多
文摘Objective:To synthesize nanoformulated naringenin(NF-n)and evaluate its anti-angiogenic and anticancer activities.Methods:NF-n was synthesized using the solvent evaporation method and characterized by dynamic light scattering,Fourier transform infrared spectroscopy,and scanning electron microscopy.Molecular docking studies were performed to assess NF-n’s binding affinity to vascular endothelial growth factor(VEGF).In vitro assays using HUVEC and MCF-7 cell lines were conducted to evaluate cytotoxicity and cell migration inhibition.The mRNA expression levels of angiogenesis-and inflammation-related markers(nestin,NRP-1,NRP-2,CD93,IL-1β,TNF-α,NF-κB,and Bcl-2)were quantified via RT-PCR.The anti-angiogenic effect of NF-n was further investigated using the chick chorioallantoic membrane assay.Results:Molecular docking revealed effective binding of naringenin to VEGF.NF-n demonstrated significantly reduced particle size and improved physicochemical properties.In in vitro studies,NF-n reduced cell viability and inhibited migration in both HUVEC and MCF-7 cells.RT-PCR analysis showed that NF-n significantly downregulated pro-angiogenic and inflammatory markers.Furthermore,NF-n significantly decreased blood vessel density,total branching points,and vessel length in heparin-induced chick chorioallantoic membrane.Conclusions:NF-n exhibits anti-angiogenic and anticancer properties,positioning it as a promising candidate for therapeutic application in cancer and other pathological conditions involving abnormal angiogenesis.Further preclinical studies are recommended to explore its translational potential.
