Objective and Impact Statement:The microspheres were widely utilized in the field of life sciences,and we have developed an innovative microelectromechanical system(MEMS)-based bioprinting technology(MBT)system for th...Objective and Impact Statement:The microspheres were widely utilized in the field of life sciences,and we have developed an innovative microelectromechanical system(MEMS)-based bioprinting technology(MBT)system for the preparation of the microspheres.The microspheres can be automatically and high-throughput produced with this cutting-edge system.Introduction and Methods:This paper mainly introduced a novel,efficient,and cost-effective approach for the microsphere fabrication with the MBT system.In this work,the whole microsphere production equipment was built and the optimal conditions(like concentration,drying temperature,frequency,and voltage)for generating uniform hydroxypropyl cellulose-cyclosporine A(HPC-CsA)and poly-l-lactic acid(PLLA)microspheres were explored.Results:Results demonstrated that the optimal uniformity of HPC-CsA microspheres was achieved at 2%(w/v)HPC-CsA mixture,45°C(drying temperature),1,000 Hz(frequency),and 25 V(voltage amplitude).CsA microspheres[coefficient of variation(CV):~9%]are successfully synthesized,and the drug encapsulation rate was 84.8%.The methodology was further used to produce PLLA microspheres with a diameter of~2.55μm,and the best CV value achieved 6.84%.Conclusion:This investigation fully highlighted the integration of MEMS and bioprinting as a promising tool for the microsphere fabrication,and this MBT system had huge potential applications in pharmaceutical formulations and medical aesthetics.展开更多
基金sponsored by Shanghai Pujiang Program(23PJ1423400)Shanghai Rising-Star Program(23QB1405100)National Key Research and Development Program of China(2019YFA0707004 and SQ2021YFF0700202).
文摘Objective and Impact Statement:The microspheres were widely utilized in the field of life sciences,and we have developed an innovative microelectromechanical system(MEMS)-based bioprinting technology(MBT)system for the preparation of the microspheres.The microspheres can be automatically and high-throughput produced with this cutting-edge system.Introduction and Methods:This paper mainly introduced a novel,efficient,and cost-effective approach for the microsphere fabrication with the MBT system.In this work,the whole microsphere production equipment was built and the optimal conditions(like concentration,drying temperature,frequency,and voltage)for generating uniform hydroxypropyl cellulose-cyclosporine A(HPC-CsA)and poly-l-lactic acid(PLLA)microspheres were explored.Results:Results demonstrated that the optimal uniformity of HPC-CsA microspheres was achieved at 2%(w/v)HPC-CsA mixture,45°C(drying temperature),1,000 Hz(frequency),and 25 V(voltage amplitude).CsA microspheres[coefficient of variation(CV):~9%]are successfully synthesized,and the drug encapsulation rate was 84.8%.The methodology was further used to produce PLLA microspheres with a diameter of~2.55μm,and the best CV value achieved 6.84%.Conclusion:This investigation fully highlighted the integration of MEMS and bioprinting as a promising tool for the microsphere fabrication,and this MBT system had huge potential applications in pharmaceutical formulations and medical aesthetics.
