以赛里木拉丝酸奶中提取的瑞士乳杆菌MB 2-1(Lactobacillus helveticus MB 2-1)为研究对象,探讨MTT法用于细菌计数的可行性及测量细菌数量的范围,优化MTT法用于瑞士乳杆菌活菌计数的工作波长、MTT和DMSO用量、反应时间。结果表明:在107...以赛里木拉丝酸奶中提取的瑞士乳杆菌MB 2-1(Lactobacillus helveticus MB 2-1)为研究对象,探讨MTT法用于细菌计数的可行性及测量细菌数量的范围,优化MTT法用于瑞士乳杆菌活菌计数的工作波长、MTT和DMSO用量、反应时间。结果表明:在107～109CFU/mL内测出的OD值与细菌浓度呈正向线性关系(R2>0.99),最佳反应条件为:1.0mL待测菌液与0.2mL MTT溶液在40℃条件下染色反应1h,反应液需要经过12000r/min离心10min,取离心沉淀物溶解于1.0mL二甲基亚砜中,于工作波长510nm处测定此溶液的OD值。MTT比色分析法可用于检测乳酸菌活菌数量。展开更多
We try to give a quantitative and global discrimination function by studying mb/MS data using Fisher method that is a kind of pattern recognition methods. The reliability of the function is also analyzed. The results ...We try to give a quantitative and global discrimination function by studying mb/MS data using Fisher method that is a kind of pattern recognition methods. The reliability of the function is also analyzed. The results show that this criterion works well and has a global feature, which can be used as first-level filtering criterions in event identification. The quantitative and linear discrimination function makes it possible to identify events automatically and achieve the goal to react the events quickly and effectively.展开更多
The simulation of real contact area between materials is foundationally important for the contact mechanics of mechanical structures. The Greenwood and Williamson(GW) model and the Majumdar(MB) model are the basic mod...The simulation of real contact area between materials is foundationally important for the contact mechanics of mechanical structures. The Greenwood and Williamson(GW) model and the Majumdar(MB) model are the basic models in this field, which are widely accepted and proven to be valid in many experiments and engineering. Although the contact models have evolved considerably in recent years, the verifications of the models are most based on the indirect methods such as electrical conductivity and contact stiffness, because of the lack of effective methods to directly measure the variation of contact surface. In this paper, the total reflection(TR) method is introduced into the verification of contact models.An experiment system based on TR method is constructed to measure the real contact area of two PMMA specimens. The comparison analysis between the results of experiment and models suggests that the experiment result has the same trend with simulation, the MB model has better agreement with the experimental result because this method can take into account the variation of radius and the merging of asperities, while the GW model has a huge deviation because of the dependence on resolution and the lack of considering the variation of radius and asperity's merging process. Taking the interaction of asperities into account could give a better result that is closer to the experiment. Our results and analysis prove that the experimental methods in this paper could be used as a more direct and valid method to quantitatively measure the real contact area and to verify the contact models.展开更多
基金Contribution No.05FE3018,Institute of Geophysics,China Earthquake Administrstion
文摘We try to give a quantitative and global discrimination function by studying mb/MS data using Fisher method that is a kind of pattern recognition methods. The reliability of the function is also analyzed. The results show that this criterion works well and has a global feature, which can be used as first-level filtering criterions in event identification. The quantitative and linear discrimination function makes it possible to identify events automatically and achieve the goal to react the events quickly and effectively.
基金Project supported by the National Natural Science Foundation of China(Grant No.11872033)the Beijing Natural Science Foundation,China(Grant No.3172017)
文摘The simulation of real contact area between materials is foundationally important for the contact mechanics of mechanical structures. The Greenwood and Williamson(GW) model and the Majumdar(MB) model are the basic models in this field, which are widely accepted and proven to be valid in many experiments and engineering. Although the contact models have evolved considerably in recent years, the verifications of the models are most based on the indirect methods such as electrical conductivity and contact stiffness, because of the lack of effective methods to directly measure the variation of contact surface. In this paper, the total reflection(TR) method is introduced into the verification of contact models.An experiment system based on TR method is constructed to measure the real contact area of two PMMA specimens. The comparison analysis between the results of experiment and models suggests that the experiment result has the same trend with simulation, the MB model has better agreement with the experimental result because this method can take into account the variation of radius and the merging of asperities, while the GW model has a huge deviation because of the dependence on resolution and the lack of considering the variation of radius and asperity's merging process. Taking the interaction of asperities into account could give a better result that is closer to the experiment. Our results and analysis prove that the experimental methods in this paper could be used as a more direct and valid method to quantitatively measure the real contact area and to verify the contact models.
