Background:Nucleolar protein 6(NOL6)is a nucleolar RNA-associated protein that is highly conserved between species.It has been proved to be associated with the prognosis of liver cancer.However,the underlying mechanis...Background:Nucleolar protein 6(NOL6)is a nucleolar RNA-associated protein that is highly conserved between species.It has been proved to be associated with the prognosis of liver cancer.However,the underlying mechanism has not been fully established.This study aimed to assess the relationship between NOL6 and liver cancer prognosis.Methods:We constructed anNOL6-short hairpin RNA(shRNA)-expressing lentivirus.Through viral transfection,cell growth assay and fluorescence-activated cell sorting,we evaluated the effect of shRNA-mediatedNOL6 knockdown on the proliferation,colony formation,and apoptosis of hepatocellular carcinoma(HCC)cells.The relationship betweenNOL6 expression and HCC patient survival has been established through bioinformatics analysis.We also explored the downstream molecular regulatory network ofNOL6 in HCC by performing an Ingenuity Pathway Analysis in the database.Results:IncreasedNOL6 expression was detected in HCC cells compared to normal controls;HCC patients with highNOL6 expression had poorer prognoses than those with low expression.NOL6 knockdown inhibited HCC cell proliferation,apoptosis,and colony formation.Also,MAPK8,CEBPA,andFOSL1 were selected as potential downstream genes ofNOL6.Conclusions:NOL6 up-regulates HCC cell proliferation and affects downstream expression of related genes.Moreover,NOL6 is considered to be associated with poor prognosis in HCC patients.展开更多
Uncontrolled and chronic microglia activation has been implicated in the process of dopaminergic neuron degeneration in sporadic Parkinson＇s disease （PD）. Elevated proinflammatory mediators, presumably from activat...Uncontrolled and chronic microglia activation has been implicated in the process of dopaminergic neuron degeneration in sporadic Parkinson＇s disease （PD）. Elevated proinflammatory mediators, presumably from activated microglia （e.g., cytokines, PGE2, nitric oxide, and superoxide radical）, have been observed in PD patients and are accompanied by dopaminergic neuronal loss. Preclinical studies have demonstrated the deleterious effects of proinflammatory mediators in various in vivo and in vitro models of PD. The use of in vitro studies provides a unique tool to investigate the interaction between neurons and microglia and is especially valuable when considering the role of activated microglia in neuronal death. Here we summarize findings highlighting the potential mechanisms of microglia- mediated neurodegeneration in PD.展开更多
基金supported by a grant from the Natural Science Foundation(No.81370069)。
文摘Background:Nucleolar protein 6(NOL6)is a nucleolar RNA-associated protein that is highly conserved between species.It has been proved to be associated with the prognosis of liver cancer.However,the underlying mechanism has not been fully established.This study aimed to assess the relationship between NOL6 and liver cancer prognosis.Methods:We constructed anNOL6-short hairpin RNA(shRNA)-expressing lentivirus.Through viral transfection,cell growth assay and fluorescence-activated cell sorting,we evaluated the effect of shRNA-mediatedNOL6 knockdown on the proliferation,colony formation,and apoptosis of hepatocellular carcinoma(HCC)cells.The relationship betweenNOL6 expression and HCC patient survival has been established through bioinformatics analysis.We also explored the downstream molecular regulatory network ofNOL6 in HCC by performing an Ingenuity Pathway Analysis in the database.Results:IncreasedNOL6 expression was detected in HCC cells compared to normal controls;HCC patients with highNOL6 expression had poorer prognoses than those with low expression.NOL6 knockdown inhibited HCC cell proliferation,apoptosis,and colony formation.Also,MAPK8,CEBPA,andFOSL1 were selected as potential downstream genes ofNOL6.Conclusions:NOL6 up-regulates HCC cell proliferation and affects downstream expression of related genes.Moreover,NOL6 is considered to be associated with poor prognosis in HCC patients.
文摘Uncontrolled and chronic microglia activation has been implicated in the process of dopaminergic neuron degeneration in sporadic Parkinson＇s disease （PD）. Elevated proinflammatory mediators, presumably from activated microglia （e.g., cytokines, PGE2, nitric oxide, and superoxide radical）, have been observed in PD patients and are accompanied by dopaminergic neuronal loss. Preclinical studies have demonstrated the deleterious effects of proinflammatory mediators in various in vivo and in vitro models of PD. The use of in vitro studies provides a unique tool to investigate the interaction between neurons and microglia and is especially valuable when considering the role of activated microglia in neuronal death. Here we summarize findings highlighting the potential mechanisms of microglia- mediated neurodegeneration in PD.
