【目的】分离丽江猪丝裂原活化蛋白激酶10(mitogen-activated protein kinase,MAPK10)基因序列,并分析其组织mRNA和蛋白表达情况。【方法】采用PCR技术扩增丽江猪MAPK10基因,并利用生物信息学软件对获得的序列进行分析,实时荧光定量PCR...【目的】分离丽江猪丝裂原活化蛋白激酶10(mitogen-activated protein kinase,MAPK10)基因序列,并分析其组织mRNA和蛋白表达情况。【方法】采用PCR技术扩增丽江猪MAPK10基因,并利用生物信息学软件对获得的序列进行分析,实时荧光定量PCR检测丽江猪脑、肝脏、心脏、脾脏、肺脏、肾脏、胰脏、背脂和背最长肌等9种组织中MAPK10基因的相对表达量;以大白猪为对照,实时荧光定量PCR和Western-blotting检测丽江猪与大白猪背脂中mRNA和蛋白表达差异。【结果】MAPK10基因序列总长1281 bp,编码426个氨基酸;MAPK10蛋白属亲水蛋白,无跨膜结构和信号肽,有44个磷酸化位点和2个糖基化位点;二级结构以α-螺旋和无规则卷曲为主;丽江猪MAPK10基因序列与人、牛、山羊、绵羊、鼠、狗、猴和鸡的同源性分别为95.3%、93.5%、93.2%、93.0%、92.6%、91.2%、90.5%和85.6%;MAPK10基因在丽江猪9个组织中差异表达,以脑中最高;丽江猪背脂MAPK10基因的mRNA表达量极显著低于大白猪(P<0.01),蛋白表达量也有低于大白猪的趋势(P>0.05)。【结论】MAPK10基因可作为研究丽江猪脂肪沉积性状的候选基因,结果可为研究猪脂肪沉积提供参考。展开更多
Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.Ho...Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.However, the molecular mechanisms are not clear. Here,we found that gastrodin ameliorated pentylenetetrazole（PTZ）-induced epileptic seizures with improvement of the electroencephalographic pattern in mice. Further studies demonstrated that gastrodin decreased the levels of the pro-inflammatory cytokines interleukin-1b and tumor necrosis factor-a while increasing interleukin-10, an antiinflammatory cytokine in the brain. Furthermore, gastrodin attenuated the PTZ-induced microglial activation along with inhibition of mitogen-activated protein kinases,c AMP response element binding protein, and NF-j B. Our data suggest that gastrodin attenuates seizures by modulating the mitogen-activated protein kinase-associated inflammatory responses.展开更多
Objective:To investigate the expressions of MAPK10,c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel,cisplatin and 5-fluorouracil(TPF)chemotherapy.Methods:Fifty-seven patients wi...Objective:To investigate the expressions of MAPK10,c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel,cisplatin and 5-fluorouracil(TPF)chemotherapy.Methods:Fifty-seven patients with supraglottic squamous cell carcinoma,who were treated by two cycles of TPF induction chemotherapy in our hospital,were enrolled in this study and divided into groups by chemotherapy resistance or chemotherapy sensitivity.The expressions of mRNA and protein of MAPK10,c-Jun and Itga6 in tumor tissues were evaluated by immunohistochemistry.The consistency of mRNA and protein expressions was tested,and the relation with the clinicopathological features was analyzed.Results:The positive rates of MAPK10 andc-Jun in the tumor tissues of the sensitive group were significantly higher than those of there assistant group,which was 90.48%and 100.00%,respectively.The expression rate of Itga6 was significantly higher in the resistant group,which was 83.33%(P<0.05).The mRNA levels of MAPK10 and c-Jun were significantly lower in the resistant group than in the sensitive group,whilethemRNA levelof Itga6was significantly higher in the resistant group(P<0.05).The protein expressions of MAPK10,c-Jun and Itga6 were consistent with their mRNA expressions(P<0.05).The expressions of MAPK10,c-Jun and Itga6 were not correlatedwithage,gender and tumor diameter(P>0.05).However,the expressions of MAPK10 and c-Jun were negatively correlated withclinical stage and pathological grading(P<0.05).Negative correlations between MAPK 10 and Itga6,and between c-Jun and Itga6in tumor tissues were found by Spearman’srank correlation coefficient(P<0.05).The correlation was also negative in the resistant tumor tissues(P<0.05).Conclusion:The MAPK10 and c-Jun expressions were down-regulated,while the Itga6 expression was up-regulated in the chemo-resistant laryngeal carcinoma,and the expression levels of different factors were correlated witheach other.These factorsmight be important biomarkers for predicting outcomes of TPF chemotherapy in laryngeal carcinoma in the future.展开更多
文摘【目的】分离丽江猪丝裂原活化蛋白激酶10(mitogen-activated protein kinase,MAPK10)基因序列,并分析其组织mRNA和蛋白表达情况。【方法】采用PCR技术扩增丽江猪MAPK10基因,并利用生物信息学软件对获得的序列进行分析,实时荧光定量PCR检测丽江猪脑、肝脏、心脏、脾脏、肺脏、肾脏、胰脏、背脂和背最长肌等9种组织中MAPK10基因的相对表达量;以大白猪为对照,实时荧光定量PCR和Western-blotting检测丽江猪与大白猪背脂中mRNA和蛋白表达差异。【结果】MAPK10基因序列总长1281 bp,编码426个氨基酸;MAPK10蛋白属亲水蛋白,无跨膜结构和信号肽,有44个磷酸化位点和2个糖基化位点;二级结构以α-螺旋和无规则卷曲为主;丽江猪MAPK10基因序列与人、牛、山羊、绵羊、鼠、狗、猴和鸡的同源性分别为95.3%、93.5%、93.2%、93.0%、92.6%、91.2%、90.5%和85.6%;MAPK10基因在丽江猪9个组织中差异表达,以脑中最高;丽江猪背脂MAPK10基因的mRNA表达量极显著低于大白猪(P<0.01),蛋白表达量也有低于大白猪的趋势(P>0.05)。【结论】MAPK10基因可作为研究丽江猪脂肪沉积性状的候选基因,结果可为研究猪脂肪沉积提供参考。
文摘Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.However, the molecular mechanisms are not clear. Here,we found that gastrodin ameliorated pentylenetetrazole（PTZ）-induced epileptic seizures with improvement of the electroencephalographic pattern in mice. Further studies demonstrated that gastrodin decreased the levels of the pro-inflammatory cytokines interleukin-1b and tumor necrosis factor-a while increasing interleukin-10, an antiinflammatory cytokine in the brain. Furthermore, gastrodin attenuated the PTZ-induced microglial activation along with inhibition of mitogen-activated protein kinases,c AMP response element binding protein, and NF-j B. Our data suggest that gastrodin attenuates seizures by modulating the mitogen-activated protein kinase-associated inflammatory responses.
基金Beijing Municipal Administration of Hospitals Incubating Program(PX2018009).
文摘Objective:To investigate the expressions of MAPK10,c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel,cisplatin and 5-fluorouracil(TPF)chemotherapy.Methods:Fifty-seven patients with supraglottic squamous cell carcinoma,who were treated by two cycles of TPF induction chemotherapy in our hospital,were enrolled in this study and divided into groups by chemotherapy resistance or chemotherapy sensitivity.The expressions of mRNA and protein of MAPK10,c-Jun and Itga6 in tumor tissues were evaluated by immunohistochemistry.The consistency of mRNA and protein expressions was tested,and the relation with the clinicopathological features was analyzed.Results:The positive rates of MAPK10 andc-Jun in the tumor tissues of the sensitive group were significantly higher than those of there assistant group,which was 90.48%and 100.00%,respectively.The expression rate of Itga6 was significantly higher in the resistant group,which was 83.33%(P<0.05).The mRNA levels of MAPK10 and c-Jun were significantly lower in the resistant group than in the sensitive group,whilethemRNA levelof Itga6was significantly higher in the resistant group(P<0.05).The protein expressions of MAPK10,c-Jun and Itga6 were consistent with their mRNA expressions(P<0.05).The expressions of MAPK10,c-Jun and Itga6 were not correlatedwithage,gender and tumor diameter(P>0.05).However,the expressions of MAPK10 and c-Jun were negatively correlated withclinical stage and pathological grading(P<0.05).Negative correlations between MAPK 10 and Itga6,and between c-Jun and Itga6in tumor tissues were found by Spearman’srank correlation coefficient(P<0.05).The correlation was also negative in the resistant tumor tissues(P<0.05).Conclusion:The MAPK10 and c-Jun expressions were down-regulated,while the Itga6 expression was up-regulated in the chemo-resistant laryngeal carcinoma,and the expression levels of different factors were correlated witheach other.These factorsmight be important biomarkers for predicting outcomes of TPF chemotherapy in laryngeal carcinoma in the future.
