Mycoplasma gallisepticum(MG) is a common avian pathogen that mainly infects poultry, causingsignificant reductions in body weight gain and egg production, along with damage to immune organs andimmunosuppression. MG is...Mycoplasma gallisepticum(MG) is a common avian pathogen that mainly infects poultry, causingsignificant reductions in body weight gain and egg production, along with damage to immune organs andimmunosuppression. MG is susceptible to co-infections with other pathogens, leading to increased mortalityrates and significant economic losses in the global poultry industry. While antibiotics have been extensivelyapplied worldwide to treat MG infections in poultry production, concerns regarding antibiotic resistanceand residue remain prevalent. Traditional Chinese medicine(TCM), renowned for its natural, safe, andnon-toxic properties, has shown significant anti-inflammatory and immune-enhancing effects. This studyaimed to investigate the protective effect of TCM on production performance and its impact on MG-inducedimmunosuppression through the MAPK/ERK/JNK signaling pathway in chickens. Our results showed that TCMalleviated the negative effects of MG infection on production performance, as evidenced by improvements inbody weight gain, feed conversion rate, survival rate, and immune organ index. TCM exhibited direct inhibitionof the MG proliferation in vitro and in vivo. Furthermore, TCM treatment promoted the normalization of tracheaand lung tissue structure in MG-infected chickens, leading to a significant reduction in inflammatory damage.Moreover, following the treatment with the TCM, the production of pro-inflammatory cytokines interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) decreased significantly, accompanied by the downregulation of pro-apoptotic genes caspase3, caspase9, and BAX, both in vitro and in vivo. A mechanism-based study showed that in vitro and in vivo treatment with the TCM significantly reduced the expression of key proteins, including early growth response gene 1(EGR1), p-ERK, p-JNK, and p-JUN. Altogether, TCM improved body weight gain, inhibited pro-inflammation responses, and alleviated tissue damage by inhibiting the MAPK/ERK/JNK signaling pathway to protect the performance and immune system of MG-infected chickens.展开更多
目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1...目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响;Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果 益气解毒方水提物能抑制CNE1、CNE2细胞增殖( P <0.05)、诱导凋亡( P <0.05);药物作用48 h后,Survivin、XIAP、Bcl-2表达下降,Bax表达上升,MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK表达下降( P <0.05);在此基础上,加入激活剂ISO和益气解毒方水提物后,与单用益气解毒方水提物相比,p-c-Raf、p-MEK、p-ERK1/2表达上调,Survivin、XIAP、Bcl-2表达增加,Bax表达下降,促凋亡效应也降低( P <0.05)。结论 益气解毒方水提物可诱导鼻咽癌细胞凋亡,该效应与其抑制MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK1/2表达,进而下调Survivin、XIAP、Bcl-2表达,上调Bax表达有关。展开更多
基金supported by the National Natural Science Foundation of China (32273010 and 31972681)the National Key Research and Development Program of China (2017YFD0501500)。
文摘Mycoplasma gallisepticum(MG) is a common avian pathogen that mainly infects poultry, causingsignificant reductions in body weight gain and egg production, along with damage to immune organs andimmunosuppression. MG is susceptible to co-infections with other pathogens, leading to increased mortalityrates and significant economic losses in the global poultry industry. While antibiotics have been extensivelyapplied worldwide to treat MG infections in poultry production, concerns regarding antibiotic resistanceand residue remain prevalent. Traditional Chinese medicine(TCM), renowned for its natural, safe, andnon-toxic properties, has shown significant anti-inflammatory and immune-enhancing effects. This studyaimed to investigate the protective effect of TCM on production performance and its impact on MG-inducedimmunosuppression through the MAPK/ERK/JNK signaling pathway in chickens. Our results showed that TCMalleviated the negative effects of MG infection on production performance, as evidenced by improvements inbody weight gain, feed conversion rate, survival rate, and immune organ index. TCM exhibited direct inhibitionof the MG proliferation in vitro and in vivo. Furthermore, TCM treatment promoted the normalization of tracheaand lung tissue structure in MG-infected chickens, leading to a significant reduction in inflammatory damage.Moreover, following the treatment with the TCM, the production of pro-inflammatory cytokines interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) decreased significantly, accompanied by the downregulation of pro-apoptotic genes caspase3, caspase9, and BAX, both in vitro and in vivo. A mechanism-based study showed that in vitro and in vivo treatment with the TCM significantly reduced the expression of key proteins, including early growth response gene 1(EGR1), p-ERK, p-JNK, and p-JUN. Altogether, TCM improved body weight gain, inhibited pro-inflammation responses, and alleviated tissue damage by inhibiting the MAPK/ERK/JNK signaling pathway to protect the performance and immune system of MG-infected chickens.
文摘目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响;Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果 益气解毒方水提物能抑制CNE1、CNE2细胞增殖( P <0.05)、诱导凋亡( P <0.05);药物作用48 h后,Survivin、XIAP、Bcl-2表达下降,Bax表达上升,MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK表达下降( P <0.05);在此基础上,加入激活剂ISO和益气解毒方水提物后,与单用益气解毒方水提物相比,p-c-Raf、p-MEK、p-ERK1/2表达上调,Survivin、XIAP、Bcl-2表达增加,Bax表达下降,促凋亡效应也降低( P <0.05)。结论 益气解毒方水提物可诱导鼻咽癌细胞凋亡,该效应与其抑制MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK1/2表达,进而下调Survivin、XIAP、Bcl-2表达,上调Bax表达有关。
