促分裂原活化蛋白激酶(Mitogen-Activated Protein Kinase, MAPK)级联途径能够将细胞外刺激传导至细胞内,在植物生长发育和逆境响应中发挥重要作用。为进一步探究大豆MAPK基因在盐胁迫响应中的功能,本研究对大豆MAPK基因家族成员进行了...促分裂原活化蛋白激酶(Mitogen-Activated Protein Kinase, MAPK)级联途径能够将细胞外刺激传导至细胞内,在植物生长发育和逆境响应中发挥重要作用。为进一步探究大豆MAPK基因在盐胁迫响应中的功能,本研究对大豆MAPK基因家族成员进行了系统进化分析、共线性分析、motif分析、基因结构分析、顺式作用元件分析、组织特异性表达分析以及NaCl、NaHCO_(3)、PEG和甘露醇胁迫下的表达分析,旨在解析MAPK基因家族的盐胁迫响应机制。通过比较不同非生物胁迫下的表达模式,证实该基因家族通过差异表达调控网络介导大豆对盐胁迫的适应性响应。结果表明:共筛选出19个大豆MAPK家族成员,分布于12条染色体上,其中16号染色体分布最多。理化性质分析表明,MAPK家族成员氨基酸长度为373~571 aa,分子量为41.03~62.81 kDa。系统进化树分析显示,大豆MAPK家族成员可分为4个亚家族。共线性、motif和基因结构分析表明,各亚家族成员间联系紧密,保守性高。启动子顺式作用元件分析发现,大豆MAPK家族成员启动子中含有多种激素和应激响应元件,推测该家族参与大豆非生物胁迫响应。已有研究证实B、C和D亚家族成员参与植物逆境响应机制,因此本研究未将这3族作为重点。表达模式分析显示,在A亚家族的6个成员中,GmMAPK23-4在NaCl、NaHCO_(3)、PEG和甘露醇胁迫下的表达量变化最为显著。本研究为MAPK基因家族的深入研究提供了理论依据,为进一步理解植物逆境适应性及提升作物耐逆性提供了重要参考。展开更多
Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we iden...Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we identified an ethylene response factor,VqERF1B,that exhibits sustained high expression during E.necator infection in Chinese wild grape Vitis quinquangularis accession ‘Danfeng-2'.Transient overexpression of VqERF1B in grape leaves enhanced resistance to E.necator by elevating transcript levels of pathogenesis-related(PR) genes,including PR1,PR2,PR5,and PR10.Conversely,silencing VqERF1B resulted in increased susceptibility.Moreover,transgenic Arabidopsis lines stably overexpressing VqERF1B exhibited enhanced resistance to powdery mildew,associated with elevated PR gene expression and increased accumulation of reactive oxygen species(ROS).A series of assays identified VqMAPK3,a phosphorylated mitogen-activated protein kinase,as a direct interactor of VqERF1B.Furthermore,VqERF1B was shown to bind directly to the promoters of VqPRs,thereby activating their transcription.Notably,the VqMAPK3-VqERF1B complex exhibited greater transactivation activity on VqPR promoters than VqERF1B alone,indicating that VqMAPK3 positively modulates VqERF1Bmediated transcription of PR genes.This work advances understanding of the molecular basis of grape resistance to E.necator and provides a foundation for molecular breeding strategies.展开更多
基金This study is granted by the National Natural Science Foundation of China(52209055,52379041,and 32272667)the Yunnan Fundamental Research Projects,China (202401AU070197,202501AW070013,202501BC070015,and 202501AT070377)+3 种基金the Yunnan Education Department Project,China (2024J0079)the Kunming University of Science and Technology Talent Development Project,China (KKZ3202423161)the Yunnan Key Laboratory of Efficient Utilization of Agricultural Water Resources and Intelligent Control,China (202449CE340014)the Yunnan Intelligent Water-Fertilizer-Pesticide Integration Technology and Equipment Innovation Team,China (202505AS350025)。
文摘Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we identified an ethylene response factor,VqERF1B,that exhibits sustained high expression during E.necator infection in Chinese wild grape Vitis quinquangularis accession ‘Danfeng-2'.Transient overexpression of VqERF1B in grape leaves enhanced resistance to E.necator by elevating transcript levels of pathogenesis-related(PR) genes,including PR1,PR2,PR5,and PR10.Conversely,silencing VqERF1B resulted in increased susceptibility.Moreover,transgenic Arabidopsis lines stably overexpressing VqERF1B exhibited enhanced resistance to powdery mildew,associated with elevated PR gene expression and increased accumulation of reactive oxygen species(ROS).A series of assays identified VqMAPK3,a phosphorylated mitogen-activated protein kinase,as a direct interactor of VqERF1B.Furthermore,VqERF1B was shown to bind directly to the promoters of VqPRs,thereby activating their transcription.Notably,the VqMAPK3-VqERF1B complex exhibited greater transactivation activity on VqPR promoters than VqERF1B alone,indicating that VqMAPK3 positively modulates VqERF1Bmediated transcription of PR genes.This work advances understanding of the molecular basis of grape resistance to E.necator and provides a foundation for molecular breeding strategies.
