N6-甲基腺苷(m6A)是RNA分子中最丰富的表观遗传修饰,m6A甲基转移酶KIAA1429(又称VIRMA)是构成m6A写入器中调控亚基的核心组分。近年研究表明,KIAA1429在多种肿瘤中发挥致癌作用,其功能机制已逐步被阐明。KIAA1429通过调控MAP3K2 m RNA、...N6-甲基腺苷(m6A)是RNA分子中最丰富的表观遗传修饰,m6A甲基转移酶KIAA1429(又称VIRMA)是构成m6A写入器中调控亚基的核心组分。近年研究表明,KIAA1429在多种肿瘤中发挥致癌作用,其功能机制已逐步被阐明。KIAA1429通过调控MAP3K2 m RNA、CHST11 m RNA等RNA的m6A修饰位点,参与肿瘤的发生发展进程。同时,其表达水平也受到上游靶点的精密调控。展开更多
T cell-mediated immunity in the intestine is stringently controlled to ensure proper immunity against pathogenic microbes and to prevent autoimmunity,a known cause of inflammatory bowel disease.However,precisely how T...T cell-mediated immunity in the intestine is stringently controlled to ensure proper immunity against pathogenic microbes and to prevent autoimmunity,a known cause of inflammatory bowel disease.However,precisely how T cells regulate intestine immunity remains to be fully understood.In this study,we found that mitogen-activated protein kinase kinase kinase 2(MAP3K2)is required for the CD4^(+)T cell-mediated inflammation in the intestine.Using a T cell transfer colitis model,we found that MAP3K2-deficient naïve CD4^(+)T cells had a dramatically reduced ability to induce colitis compared to wild type T cells.In addition,significantly fewer IFN-γ-but more IL-17A-producing CD4^(+)T cells in the intestines of mice receiving MAP3K2-deficient T cells than in those from mice receiving wild type T cells was observed.Interestingly,under well-defined in vitro differentiation conditions,MAP3K2-deficient naïve T cells were not impaired in their ability to differentiate into Th1,Th17 and Treg.Furthermore,the MAP3K2-regulated colitis severity was mediated by Th1 but not Th17 cells in the intestine.At the molecular level,we showed that MAP3K2-mediated Th1 cell differentiation in the intestine was regulated by IL-18 and required specific JNK activation.Together,our study reveals a novel regulatory role of MAP3K2 in intestinal T cell immunity via the IL-18-MAP3K2-JNK axis and may provide a novel target for intervention in T cell-mediated colitis.展开更多
文摘N6-甲基腺苷(m6A)是RNA分子中最丰富的表观遗传修饰,m6A甲基转移酶KIAA1429(又称VIRMA)是构成m6A写入器中调控亚基的核心组分。近年研究表明,KIAA1429在多种肿瘤中发挥致癌作用,其功能机制已逐步被阐明。KIAA1429通过调控MAP3K2 m RNA、CHST11 m RNA等RNA的m6A修饰位点,参与肿瘤的发生发展进程。同时,其表达水平也受到上游靶点的精密调控。
基金the National Natural Science Foundation of China(81430033,81871269,91942311 and 31930035)。
文摘T cell-mediated immunity in the intestine is stringently controlled to ensure proper immunity against pathogenic microbes and to prevent autoimmunity,a known cause of inflammatory bowel disease.However,precisely how T cells regulate intestine immunity remains to be fully understood.In this study,we found that mitogen-activated protein kinase kinase kinase 2(MAP3K2)is required for the CD4^(+)T cell-mediated inflammation in the intestine.Using a T cell transfer colitis model,we found that MAP3K2-deficient naïve CD4^(+)T cells had a dramatically reduced ability to induce colitis compared to wild type T cells.In addition,significantly fewer IFN-γ-but more IL-17A-producing CD4^(+)T cells in the intestines of mice receiving MAP3K2-deficient T cells than in those from mice receiving wild type T cells was observed.Interestingly,under well-defined in vitro differentiation conditions,MAP3K2-deficient naïve T cells were not impaired in their ability to differentiate into Th1,Th17 and Treg.Furthermore,the MAP3K2-regulated colitis severity was mediated by Th1 but not Th17 cells in the intestine.At the molecular level,we showed that MAP3K2-mediated Th1 cell differentiation in the intestine was regulated by IL-18 and required specific JNK activation.Together,our study reveals a novel regulatory role of MAP3K2 in intestinal T cell immunity via the IL-18-MAP3K2-JNK axis and may provide a novel target for intervention in T cell-mediated colitis.
