Objective:To improve the efficiency of drug delivery,a mannose vinyl stearate mannose ligand(Man ligand)with active liver-targeting properties was synthesized.Methods:Non-aqueous enzymatic synthesis was used to modify...Objective:To improve the efficiency of drug delivery,a mannose vinyl stearate mannose ligand(Man ligand)with active liver-targeting properties was synthesized.Methods:Non-aqueous enzymatic synthesis was used to modify the structure of mannose.Glycyrrhetinic acid-tanshinone lipid nanoparticles(GT-LN)and liver-targeted glycyrrhetinic acid-tanshinone mannose-modified lipid nanoparticles(GT-MLN)were prepared.The physicochemical properties and release profiles of both formulations were evaluated,and their pharmacokinetic behavior and tissue distribution were investigated.Results:The average particle sizes of GT-LN and GT-MLN were 190.20±1.35 and 204.83±3.86 nm,respectively,with corresponding surface Zeta potentials of-28.0±1.68 and-30.24±2.10 mV.The drug release profile of GT-LN conformed to the Higuchi equation,whereas that of GT-MLN followed both the first-order kinetic and RitgerePeppas equations.Both formulations significantly enhanced the gastrointestinal stability of the drug.In vivo studies in mice demonstrated that hepatic GA and TSN concentrations in both groups were significantly higher than those in the original drug suspension group(P=.01).Notably,the concentrations in the GT-MLN group were significantly higher compared to the GTLN group(P=.01).Conclusion:Man ligand was formed via the linkage of vinyl stearate with the hydroxyl group at C-6 in mannose.The Manligand endowed these lipid nanoparticles with obvious active liver-targeting properties.Our results provide an efficient and stable route of drug delivery to the liver with improved drug availability.展开更多
【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探...【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探针,优化反应体系中引物和探针的浓度,建立Taq Man RT-qPCR检测方法,并通过特异性、灵敏性和重复性的结果以及临床应用对该方法进行评价。【结果】该方法可特异性扩增PoRV核酸,最低检出限度为27.0 copies·μL^(-1),灵敏度高于普通RT-PCR 100倍;与猪流行性腹泻病毒(porcine epidemicdiarrheavirus,PEDV)、猪德尔塔冠状病毒(porcinedeltacoronavirus,PDCoV)、猪传染性胃肠炎病毒(transmissible gastroenteritis of swine, TGEV)核酸均无交叉反应;组内和组间变异系数均小于1.10%,重复性好。151份疑似PoRV的临床样品使用RT-qPCR进行检测,结果显示检出率为42.38%(64/151),优于常规RT-PCR的检出率(33.11%,50/151)。【结论】本研究基于猪A群轮状病毒VP6基因,建立了适用于PoRV A检测及其流行病学调查的Taq Man实时荧光定量PCR检测方法,具有灵敏度高、特异性强、重复性好等优势,为猪轮状病毒检测和流行病学调查提供了技术手段。展开更多
Scientific and precise evaluations of the megafaunal and landform characteristics of seamounts are important guides for their protection and study.A series of manned and unmanned submersibles have provided invaluable ...Scientific and precise evaluations of the megafaunal and landform characteristics of seamounts are important guides for their protection and study.A series of manned and unmanned submersibles have provided invaluable observational imaging data for the ecological study of seamounts.However,traditional methods of artificial observation of seamount imaging data cannot accurately and efficiently determine the characteristics of megafauna and landforms.This research harnesses data-driven technology to systematically investigate the distributional traits and morphological features of megafaunal organisms,as well as the topographical characteristics,in the Caiwei Guyot region of the western Pacific’s Magellan Seamounts.To construct the landform and megafauna dataset of the Caiwei Guyot region,we used a data preprocessing technology based on image enhancement to provide high-quality imaging data for data-driven technologies.A megafaunal identification and counting algorithm based on YOLOv5(You Only Look Once Version 5)was developed to efficiently assess the abundance,variety,and dominant species of megafauna.Simultaneously,a landform three-dimensional(3D)reconstruction algorithm based on PatchmatchNet was developed to reconstruct the 3D form of the terrain accurately.This study pioneers the application of data-driven technology to deep-sea imaging within the Caiwei Guyot region,offering an innovative approach to accurately and efficiently characterize the region’s unique megafauna and landforms.展开更多
Shanghai Disney Resort broke ground on a new Spider⁃Man⁃themed land,a major expansion that represents a rising global investment interest in Chinas theme park sector.Located close to Zootopia,the new land includes the...Shanghai Disney Resort broke ground on a new Spider⁃Man⁃themed land,a major expansion that represents a rising global investment interest in Chinas theme park sector.Located close to Zootopia,the new land includes the parks first major Marvel⁃themed attraction-a high⁃energy thrill roller coaster featuring Spider⁃Man,one of the most popular Marvel superheroes,according to the company.展开更多
Invisible Man is a novel written by Ralph Ellison,talking about how the protagonist struggled in society as an African-American,sought his own identity and sense of belonging,and finally accepted the reality of being...Invisible Man is a novel written by Ralph Ellison,talking about how the protagonist struggled in society as an African-American,sought his own identity and sense of belonging,and finally accepted the reality of being“invisible”to live underground.The metaphors and symbols as key writing techniques in Invisible Man were analyzed in the paper with certain typical examples to reveal their research values for explorations of race issues in the book.The paper particularly highlights the tension embedded in the metaphors and the sarcasm conveyed through the symbols.展开更多
Since modern Saudi novel vividly captures the essence of cultural and social life in Saudi Arabia,this paper aims to unveil the manifestation of historical events that generated new issues and discourses shaping moder...Since modern Saudi novel vividly captures the essence of cultural and social life in Saudi Arabia,this paper aims to unveil the manifestation of historical events that generated new issues and discourses shaping modern Saudi culture and society as depicted in Aziz Mohammed’s The Critical Case of a Man Called K(2021).The examination of its cultural and social context,thus,serves as an evaluation of a historical record that depicts the episteme of modern Saudi society.However,as ample as previous works on Saudi literature may be,most of them disregard the use of New Historicism in examining modern Saudi novels.This paper,therefore,utilizes New Historicism to unveil the nature of modern Saudi episteme as depicted by the young author Aziz Mohammed in The Critical Case of a Man Called K(2021)and to gain insight into modern Saudi discourses and issues shaped by historical and economic events.The study finds that the novel depicts a dystopian reality due to the implications of both late capitalism and globalization in modern Saudi Arabia.Furthermore,the novel highlights how far the discourse of Saudi Vision 2030,with its various aims,penetrates both society and culture,shaping a great part of modern Saudi episteme.The paper concludes by affirming Mohammed’s novel assertion of New Historicism’s main assumption that the text cannot be divorced from its historical,cultural,and social context and that it serves as a record indicative of a specific and unique episteme.展开更多
基金the High-level construction discipline of the National Administration of Traditional Chinese Medicine(zyyzdxk-2023272).
文摘Objective:To improve the efficiency of drug delivery,a mannose vinyl stearate mannose ligand(Man ligand)with active liver-targeting properties was synthesized.Methods:Non-aqueous enzymatic synthesis was used to modify the structure of mannose.Glycyrrhetinic acid-tanshinone lipid nanoparticles(GT-LN)and liver-targeted glycyrrhetinic acid-tanshinone mannose-modified lipid nanoparticles(GT-MLN)were prepared.The physicochemical properties and release profiles of both formulations were evaluated,and their pharmacokinetic behavior and tissue distribution were investigated.Results:The average particle sizes of GT-LN and GT-MLN were 190.20±1.35 and 204.83±3.86 nm,respectively,with corresponding surface Zeta potentials of-28.0±1.68 and-30.24±2.10 mV.The drug release profile of GT-LN conformed to the Higuchi equation,whereas that of GT-MLN followed both the first-order kinetic and RitgerePeppas equations.Both formulations significantly enhanced the gastrointestinal stability of the drug.In vivo studies in mice demonstrated that hepatic GA and TSN concentrations in both groups were significantly higher than those in the original drug suspension group(P=.01).Notably,the concentrations in the GT-MLN group were significantly higher compared to the GTLN group(P=.01).Conclusion:Man ligand was formed via the linkage of vinyl stearate with the hydroxyl group at C-6 in mannose.The Manligand endowed these lipid nanoparticles with obvious active liver-targeting properties.Our results provide an efficient and stable route of drug delivery to the liver with improved drug availability.
文摘【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探针,优化反应体系中引物和探针的浓度,建立Taq Man RT-qPCR检测方法,并通过特异性、灵敏性和重复性的结果以及临床应用对该方法进行评价。【结果】该方法可特异性扩增PoRV核酸,最低检出限度为27.0 copies·μL^(-1),灵敏度高于普通RT-PCR 100倍;与猪流行性腹泻病毒(porcine epidemicdiarrheavirus,PEDV)、猪德尔塔冠状病毒(porcinedeltacoronavirus,PDCoV)、猪传染性胃肠炎病毒(transmissible gastroenteritis of swine, TGEV)核酸均无交叉反应;组内和组间变异系数均小于1.10%,重复性好。151份疑似PoRV的临床样品使用RT-qPCR进行检测,结果显示检出率为42.38%(64/151),优于常规RT-PCR的检出率(33.11%,50/151)。【结论】本研究基于猪A群轮状病毒VP6基因,建立了适用于PoRV A检测及其流行病学调查的Taq Man实时荧光定量PCR检测方法,具有灵敏度高、特异性强、重复性好等优势,为猪轮状病毒检测和流行病学调查提供了技术手段。
基金The Key Research and Development Program of Shandong Province of China under contract No.2020JMRH0101the National Key Research and Development Project of China under contract No.2021YFC2802100the Qingdao Natural Science Foundation under contract No.24-4-4-zrij-127-jch.
文摘Scientific and precise evaluations of the megafaunal and landform characteristics of seamounts are important guides for their protection and study.A series of manned and unmanned submersibles have provided invaluable observational imaging data for the ecological study of seamounts.However,traditional methods of artificial observation of seamount imaging data cannot accurately and efficiently determine the characteristics of megafauna and landforms.This research harnesses data-driven technology to systematically investigate the distributional traits and morphological features of megafaunal organisms,as well as the topographical characteristics,in the Caiwei Guyot region of the western Pacific’s Magellan Seamounts.To construct the landform and megafauna dataset of the Caiwei Guyot region,we used a data preprocessing technology based on image enhancement to provide high-quality imaging data for data-driven technologies.A megafaunal identification and counting algorithm based on YOLOv5(You Only Look Once Version 5)was developed to efficiently assess the abundance,variety,and dominant species of megafauna.Simultaneously,a landform three-dimensional(3D)reconstruction algorithm based on PatchmatchNet was developed to reconstruct the 3D form of the terrain accurately.This study pioneers the application of data-driven technology to deep-sea imaging within the Caiwei Guyot region,offering an innovative approach to accurately and efficiently characterize the region’s unique megafauna and landforms.
文摘Shanghai Disney Resort broke ground on a new Spider⁃Man⁃themed land,a major expansion that represents a rising global investment interest in Chinas theme park sector.Located close to Zootopia,the new land includes the parks first major Marvel⁃themed attraction-a high⁃energy thrill roller coaster featuring Spider⁃Man,one of the most popular Marvel superheroes,according to the company.
文摘Invisible Man is a novel written by Ralph Ellison,talking about how the protagonist struggled in society as an African-American,sought his own identity and sense of belonging,and finally accepted the reality of being“invisible”to live underground.The metaphors and symbols as key writing techniques in Invisible Man were analyzed in the paper with certain typical examples to reveal their research values for explorations of race issues in the book.The paper particularly highlights the tension embedded in the metaphors and the sarcasm conveyed through the symbols.
文摘Since modern Saudi novel vividly captures the essence of cultural and social life in Saudi Arabia,this paper aims to unveil the manifestation of historical events that generated new issues and discourses shaping modern Saudi culture and society as depicted in Aziz Mohammed’s The Critical Case of a Man Called K(2021).The examination of its cultural and social context,thus,serves as an evaluation of a historical record that depicts the episteme of modern Saudi society.However,as ample as previous works on Saudi literature may be,most of them disregard the use of New Historicism in examining modern Saudi novels.This paper,therefore,utilizes New Historicism to unveil the nature of modern Saudi episteme as depicted by the young author Aziz Mohammed in The Critical Case of a Man Called K(2021)and to gain insight into modern Saudi discourses and issues shaped by historical and economic events.The study finds that the novel depicts a dystopian reality due to the implications of both late capitalism and globalization in modern Saudi Arabia.Furthermore,the novel highlights how far the discourse of Saudi Vision 2030,with its various aims,penetrates both society and culture,shaping a great part of modern Saudi episteme.The paper concludes by affirming Mohammed’s novel assertion of New Historicism’s main assumption that the text cannot be divorced from its historical,cultural,and social context and that it serves as a record indicative of a specific and unique episteme.
