Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other q...Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.展开更多
Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].M...Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].Moreover,lipids are biomarkers for many diseases,including coronary heart disease,hepatitis C,and diabetes.A substantial number of studies have shown that obtaining accurate and comprehensive data on the spatial distribution of lipids is essential for investigating pathogenesis and identifying diagnostic biomarkers[2].展开更多
Triclocarban(TCC)is a common antimicrobial agent that has been widely used in medical care.Given the close association between TCC treatment and metabolic disorders,we assessed whether long-term treatment to TCC at a ...Triclocarban(TCC)is a common antimicrobial agent that has been widely used in medical care.Given the close association between TCC treatment and metabolic disorders,we assessed whether long-term treatment to TCC at a human-relevant concentration could induce nephrotoxicity by disrupting the metabolic levels in a mouse model.Matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)was applied to investigate the alterations in the spatial distributions and abundances of TCC,endogenous and exogenous metabolites in the kidney after TCC treatment.The results showed that TCC treatment induced the changes in the organ weight,organ coefficient and histopathology of the mouse kidney.MSI data revealed that TCC accumulated in all regions of the kidney,while its five metabolites mainly distributed in the cortex regions.The abundances of 79 biomolecules associated with pathways of leukotriene E4 metabolism,biosynthesis and degradation of glycerophospholipids and glycerolipids,ceramide-to-sphingomyelin signaling were significantly altered in the kidney after TCC treatment.These biomolecules showed distinctive distributions in the kidney and displayed a favorable spatial correlation with the pathological damage.This work offers new insights into the related mechanisms of TCC-induced nephrotocicity and exhibits the potential of MALDI-MSI-based spatial metabolomics as a promising approach for the risk assessment of agents in medical care.展开更多
A novel method for enhanced resolution,termed expansion mass spectrometry imaging,has been developed for lipid mass spectrometry imaging,utilizing existing commercially available mass spectrometers without necessitati...A novel method for enhanced resolution,termed expansion mass spectrometry imaging,has been developed for lipid mass spectrometry imaging,utilizing existing commercially available mass spectrometers without necessitating modifications.This approach involves embedding tissue sections in a swellable polyelectrolyte gel,with the target biomolecules indirectly anchored to the gel network.By employing matrix-assisted laser desorption ionization mass spectrometry imaging,the method has realized an enhanced spatial resolution that surpasses the conventional resolution limits of commercial instruments by approximately 4.5 fold.This enhancement permits the detailed visualization of intricate structures within the mouse brain at a subcellular level,with a lateral resolution nearing 1μm.As a physical technique for achieving resolution beyond standard capabilities,this readily adaptable approach presents a powerful tool for high-definition imaging in biological research.展开更多
基金supported by the National Natural Science Foundation of China (No. 21205041)the Fundamental Research Funds for the Central Universities (No. 222201314039)a grant from Shanghai Municipal Education Committee (No. YJ0114209)
文摘Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.
基金supported by the Key Research and Development Program of Zhejiang Province(2025C01135)the National Natural Science Foundation of China(31770384,21605164)+2 种基金the Youth Academic Team Project of Minzu University of China(10301-02200301)the Huayi Technology Innovation Center for Research Resources(HTIC P01 RR2017001A)the Key Laboratory Construction Funds of State Ethnic Affairs Commission of China(10301-02200303)。
文摘Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].Moreover,lipids are biomarkers for many diseases,including coronary heart disease,hepatitis C,and diabetes.A substantial number of studies have shown that obtaining accurate and comprehensive data on the spatial distribution of lipids is essential for investigating pathogenesis and identifying diagnostic biomarkers[2].
基金supported by National Natural Science Foundation of China(Grant Nos.:22036001 and 22276034).
文摘Triclocarban(TCC)is a common antimicrobial agent that has been widely used in medical care.Given the close association between TCC treatment and metabolic disorders,we assessed whether long-term treatment to TCC at a human-relevant concentration could induce nephrotoxicity by disrupting the metabolic levels in a mouse model.Matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)was applied to investigate the alterations in the spatial distributions and abundances of TCC,endogenous and exogenous metabolites in the kidney after TCC treatment.The results showed that TCC treatment induced the changes in the organ weight,organ coefficient and histopathology of the mouse kidney.MSI data revealed that TCC accumulated in all regions of the kidney,while its five metabolites mainly distributed in the cortex regions.The abundances of 79 biomolecules associated with pathways of leukotriene E4 metabolism,biosynthesis and degradation of glycerophospholipids and glycerolipids,ceramide-to-sphingomyelin signaling were significantly altered in the kidney after TCC treatment.These biomolecules showed distinctive distributions in the kidney and displayed a favorable spatial correlation with the pathological damage.This work offers new insights into the related mechanisms of TCC-induced nephrotocicity and exhibits the potential of MALDI-MSI-based spatial metabolomics as a promising approach for the risk assessment of agents in medical care.
基金supported by the General Research Fund(grant no.12302122)of the Research Grants CouncilHong Kong Special Administrative Region,China,the Start-up Grant from Hong Kong Baptist University,and the State Key Laboratory of Environmental and Biological Analysis Research Grant(grant no.SKLP_2021_P04)。
文摘A novel method for enhanced resolution,termed expansion mass spectrometry imaging,has been developed for lipid mass spectrometry imaging,utilizing existing commercially available mass spectrometers without necessitating modifications.This approach involves embedding tissue sections in a swellable polyelectrolyte gel,with the target biomolecules indirectly anchored to the gel network.By employing matrix-assisted laser desorption ionization mass spectrometry imaging,the method has realized an enhanced spatial resolution that surpasses the conventional resolution limits of commercial instruments by approximately 4.5 fold.This enhancement permits the detailed visualization of intricate structures within the mouse brain at a subcellular level,with a lateral resolution nearing 1μm.As a physical technique for achieving resolution beyond standard capabilities,this readily adaptable approach presents a powerful tool for high-definition imaging in biological research.
