我得承认,我是个愚钝且晚熟的家伙,直到我成功踏上摇滚这趟贼船,并开始处处以一个文艺青年的高标准要求自己的时候,我才知道那本叫做《百年孤独》的书,在那之前,关于哥伦比亚这个国家的所有记忆都只与足球有关,金毛狮王的大名远比... 我得承认,我是个愚钝且晚熟的家伙,直到我成功踏上摇滚这趟贼船,并开始处处以一个文艺青年的高标准要求自己的时候,我才知道那本叫做《百年孤独》的书,在那之前,关于哥伦比亚这个国家的所有记忆都只与足球有关,金毛狮王的大名远比什么魔幻现实主义来的如雷贯耳,然而比这更可惜的是,当我发现如今文艺仍未成功青年亦早已不再的时候,关于这个国家我第一个想起来的仍然还是足球--他们刚刚战胜了不可一世的阿根廷,天啊,这真是太魔幻现实主义了,不过,现在我知道了Las malas amistades,起码在接下来我趴在电脑上完成这篇文字的数个小时内,这个名宇成功的排到了《百年孤独》的前面.……展开更多
Metformin is known to be an effective treatment for patients with type 2 diabetes mellitus. Metformin induced lactic acidosis (MALA) is a serious illness which should identify and treat urgently. In this review articl...Metformin is known to be an effective treatment for patients with type 2 diabetes mellitus. Metformin induced lactic acidosis (MALA) is a serious illness which should identify and treat urgently. In this review article, we will present our own data and review the literature to highlight the importance of recognizing MALA;causes, presentations and the proper treatment, even though there are few published studies discussing MALA treatment modalities. The two cases that we presented here were patients admitted to the ICU and undergoing hemodynamic instability, which improved after removal of Metformin and clearance of associated lactic acidosis with SLED and CVVH. A case could be made for SLED and CVVH as modalities of choice for patients with MALA and hemodynamic instability, but further research is needed in this direction.展开更多
To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.Method:LO2 hepatocytes was cultured in vitro,hepatocytes were randomly divided into blank control group,25 mmol/...To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.Method:LO2 hepatocytes was cultured in vitro,hepatocytes were randomly divided into blank control group,25 mmol/L glucose solution,27 mmol/L glucose solution,29 mmol/L glucose solution,31 mmol/L glucose solution,33 mmol/L glucose solution,35 mmol/L glucose solution treatment group,after determining the optimal concentration as 31 mmol/L,use 30 mmol/L metformin solution,and then divided into blank control group,normal hepatocytes+the optimal concentration of glucose solution,normal hepatocytes+metformin solution,normal hepatocytes+.The optimal concentration of glucose solution normal hepatocytes+metformin solution,calculate the number of hepatocytes on cell count plate respectively in the 12 h,24 h,48 h,and use the lactic acid kit to determine the lactic acid value of the cell culture medium of normal liver cells+optimal concentration glucose solution and normal liver cells+optimal concentration glucose solution+metformin solution at 12 h,24 h,and 48 h,respectively.Results:There was no significant change in the lactic acid concentration but significant increase in the number of surviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Conclusions:Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stress in vitro,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminarily believed that metformin is not the direct factor leading to diabetic lactic acidosis.展开更多
Objective To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.In vitro LO2 cells,liver cells were randomly divided into blank control group,25 tendency/L glucose so...Objective To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.In vitro LO2 cells,liver cells were randomly divided into blank control group,25 tendency/L glucose solution,27 tendency/L glucose solution,29 tendency/L glucose solution,31 tendency/L glucose solution,33 tendency/L glucose solution,35 tendency/L glucose solution treatment group,the optimal concentation of 31 tendency after L,use 30 tendency for L metformin solution,and then divided into blank control group,the optimal concentration of glucose solution,normal liver cells+metformin solution normal liver cells.The optimal concentration of glucose solution normal liver cells+metformin solution respectively in the 12h,24 h,48 h on cell count plate to calculate the mumber of liver cells,and using lactic acid determination kit the optimal concentration of glucose solution+normal liver cells and normal liver cells+the optimal concentration of glucose solution+metformin solution respectively in the 12 h,24 h,48 h of cell cultures of lactic acid value.There was no significant change in the lactic acid concentration but significant increase in the number of suviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stess in vito,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminanily believed that metformin is not the direct factor leading to diabetic lactic acidosis.展开更多
文摘 我得承认,我是个愚钝且晚熟的家伙,直到我成功踏上摇滚这趟贼船,并开始处处以一个文艺青年的高标准要求自己的时候,我才知道那本叫做《百年孤独》的书,在那之前,关于哥伦比亚这个国家的所有记忆都只与足球有关,金毛狮王的大名远比什么魔幻现实主义来的如雷贯耳,然而比这更可惜的是,当我发现如今文艺仍未成功青年亦早已不再的时候,关于这个国家我第一个想起来的仍然还是足球--他们刚刚战胜了不可一世的阿根廷,天啊,这真是太魔幻现实主义了,不过,现在我知道了Las malas amistades,起码在接下来我趴在电脑上完成这篇文字的数个小时内,这个名宇成功的排到了《百年孤独》的前面.……
文摘Metformin is known to be an effective treatment for patients with type 2 diabetes mellitus. Metformin induced lactic acidosis (MALA) is a serious illness which should identify and treat urgently. In this review article, we will present our own data and review the literature to highlight the importance of recognizing MALA;causes, presentations and the proper treatment, even though there are few published studies discussing MALA treatment modalities. The two cases that we presented here were patients admitted to the ICU and undergoing hemodynamic instability, which improved after removal of Metformin and clearance of associated lactic acidosis with SLED and CVVH. A case could be made for SLED and CVVH as modalities of choice for patients with MALA and hemodynamic instability, but further research is needed in this direction.
文摘To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.Method:LO2 hepatocytes was cultured in vitro,hepatocytes were randomly divided into blank control group,25 mmol/L glucose solution,27 mmol/L glucose solution,29 mmol/L glucose solution,31 mmol/L glucose solution,33 mmol/L glucose solution,35 mmol/L glucose solution treatment group,after determining the optimal concentration as 31 mmol/L,use 30 mmol/L metformin solution,and then divided into blank control group,normal hepatocytes+the optimal concentration of glucose solution,normal hepatocytes+metformin solution,normal hepatocytes+.The optimal concentration of glucose solution normal hepatocytes+metformin solution,calculate the number of hepatocytes on cell count plate respectively in the 12 h,24 h,48 h,and use the lactic acid kit to determine the lactic acid value of the cell culture medium of normal liver cells+optimal concentration glucose solution and normal liver cells+optimal concentration glucose solution+metformin solution at 12 h,24 h,and 48 h,respectively.Results:There was no significant change in the lactic acid concentration but significant increase in the number of surviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Conclusions:Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stress in vitro,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminarily believed that metformin is not the direct factor leading to diabetic lactic acidosis.
文摘Objective To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.In vitro LO2 cells,liver cells were randomly divided into blank control group,25 tendency/L glucose solution,27 tendency/L glucose solution,29 tendency/L glucose solution,31 tendency/L glucose solution,33 tendency/L glucose solution,35 tendency/L glucose solution treatment group,the optimal concentation of 31 tendency after L,use 30 tendency for L metformin solution,and then divided into blank control group,the optimal concentration of glucose solution,normal liver cells+metformin solution normal liver cells.The optimal concentration of glucose solution normal liver cells+metformin solution respectively in the 12h,24 h,48 h on cell count plate to calculate the mumber of liver cells,and using lactic acid determination kit the optimal concentration of glucose solution+normal liver cells and normal liver cells+the optimal concentration of glucose solution+metformin solution respectively in the 12 h,24 h,48 h of cell cultures of lactic acid value.There was no significant change in the lactic acid concentration but significant increase in the number of suviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stess in vito,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminanily believed that metformin is not the direct factor leading to diabetic lactic acidosis.
