目的探讨解剖性肝切除术对肝癌患者外周血黑色素瘤抗原-1(MAGE-1)mRNA、甲胎蛋白(AFP)mRNA的影响及临床疗效。方法回顾性选取2013年3月至2015年6月治疗的原发性肝细胞癌患者117例,根据患者最终选取的手术方式分为观察组(n=69)和对照组(n...目的探讨解剖性肝切除术对肝癌患者外周血黑色素瘤抗原-1(MAGE-1)mRNA、甲胎蛋白(AFP)mRNA的影响及临床疗效。方法回顾性选取2013年3月至2015年6月治疗的原发性肝细胞癌患者117例,根据患者最终选取的手术方式分为观察组(n=69)和对照组(n=48)。观察组采用解剖性肝切除术,对照组给予非解剖性肝切除术。观察两组手术情况,术前、术后7 d的丙氨酸氨基转移酶(ALT)、谷氨酸氨基转移酶(AST)、总胆红素(TBIL)、胆碱酯酶(CHE),以及术前、术后28 d外周MAGE-1 mRNA和AFP mRNA变化,随访观察患者总体生存时间和无进展生存期。结果观察组手术时间和切缘有效率明显高于对照组(P<0.05);观察组术后7 d ALT和AST水平明显低于对照组(P<0.05);观察组和对照组术后7 d TBIL和CHE比较,差异无统计学意义(P>0.05);观察组术后28 d MAGE-1mRNA和AFP mRNA阳性率明显低于对照组(P<0.05);观察组中位总体生存时间和中位无进展生存期也明显高于对照组(P<0.05)。结论解剖性肝切除术有较好的临床效果,可改善肝功能,降低外周血MAGE-1 mRNA和AFP mRNA表达,改善患者预后。展开更多
目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1mRNA作为辅助GISTs危险度分级指标的可能性,及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例...目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1mRNA作为辅助GISTs危险度分级指标的可能性,及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例GISTs中MAGE-1和NY-ESO-1m R NA的表达,并取正常胃肠道组织作为阴性对照组,同时分析MAGE-1和NY-ESO-1mRNA表达与病理特征的关系.结果:正常对照组中无阳性表达,30例GISTs中,18例至少表达一种CTA,MAGE-1和NY-E S O-1m R NA在G I S T s中的表达率分别为30%和47%.MAGE-1和NY-ESO-1mRNA表达与患者年龄、性别和病理类型无关,而与肿瘤生长部位、肿瘤大小及危险度分级有关(P<0.05).MAGE-1和NY-ESO-1mRNA在低危、中危、高危三个组中表达量随着危险度分级的升高而增高,三组间差异有统计学意义(P<0.05).MAGE-1和NY-ESO-1mRNA在GISTs中的表达不存在相关性(r=0.018,P>0.05).结论:MAGE-1和NY-ESO-1mRNA在GISTs中的高特异性表达,其抗原有望成为GISTs免疫治疗特异性的靶点;MAGE-1和NY-ESO-1mRNA表达与GISTs危险度分级有关,MAGE-1和NY-ESO-1mRNA有望成为辅助GISTs危险度分级的诊断指标.展开更多
OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PC...OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PCR(real-time Q-PCR)assay. METHODS Peripheral blood samples were obtained from control subjects and 86 patients with hepatocellular carcinoma (HCC).Real-time Q-PCR was used to detect MAGE-1,MAGE-3, and AFP mRNAs in the blood cells. RESULTS In 86 tumor specimens,the positivity for MAGE-1, MAGE-3,and AFP genes was respectively 34.9%(30/86),60.5% (52/86)and 69.8%(60/86).All specimens expressed at least one marker.MAGE-1,MAGE-3,and AFP transcripts were detected respectively in 12(14.0%),18(20.1%)and 29(33.7%)of the 86 blood specimens from hepatocellular carcinoma patients,while 45 specimens(52.3%)were positive for at least one marker.In addition,MAGE-1,MAGE-3 and AFP gene transcripts were not detected in any peripheral blood specimens from 25 chronic liver disease patients and 28 normal healthy volunteers.The positive rate correlated with the TNM clinical stages,extrahepatic metastasis and portal vein carcinothrombosis(P<0.05).No correlation was found between tumor size,tumor number, differentiation,serum a-fetoprotein(AFP)and the positive rate. CONCLUSION Our results indicate that a multimarker real- time Q-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a hepatocyte-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.展开更多
文摘目的探讨解剖性肝切除术对肝癌患者外周血黑色素瘤抗原-1(MAGE-1)mRNA、甲胎蛋白(AFP)mRNA的影响及临床疗效。方法回顾性选取2013年3月至2015年6月治疗的原发性肝细胞癌患者117例,根据患者最终选取的手术方式分为观察组(n=69)和对照组(n=48)。观察组采用解剖性肝切除术,对照组给予非解剖性肝切除术。观察两组手术情况,术前、术后7 d的丙氨酸氨基转移酶(ALT)、谷氨酸氨基转移酶(AST)、总胆红素(TBIL)、胆碱酯酶(CHE),以及术前、术后28 d外周MAGE-1 mRNA和AFP mRNA变化,随访观察患者总体生存时间和无进展生存期。结果观察组手术时间和切缘有效率明显高于对照组(P<0.05);观察组术后7 d ALT和AST水平明显低于对照组(P<0.05);观察组和对照组术后7 d TBIL和CHE比较,差异无统计学意义(P>0.05);观察组术后28 d MAGE-1mRNA和AFP mRNA阳性率明显低于对照组(P<0.05);观察组中位总体生存时间和中位无进展生存期也明显高于对照组(P<0.05)。结论解剖性肝切除术有较好的临床效果,可改善肝功能,降低外周血MAGE-1 mRNA和AFP mRNA表达,改善患者预后。
文摘目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1mRNA作为辅助GISTs危险度分级指标的可能性,及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例GISTs中MAGE-1和NY-ESO-1m R NA的表达,并取正常胃肠道组织作为阴性对照组,同时分析MAGE-1和NY-ESO-1mRNA表达与病理特征的关系.结果:正常对照组中无阳性表达,30例GISTs中,18例至少表达一种CTA,MAGE-1和NY-E S O-1m R NA在G I S T s中的表达率分别为30%和47%.MAGE-1和NY-ESO-1mRNA表达与患者年龄、性别和病理类型无关,而与肿瘤生长部位、肿瘤大小及危险度分级有关(P<0.05).MAGE-1和NY-ESO-1mRNA在低危、中危、高危三个组中表达量随着危险度分级的升高而增高,三组间差异有统计学意义(P<0.05).MAGE-1和NY-ESO-1mRNA在GISTs中的表达不存在相关性(r=0.018,P>0.05).结论:MAGE-1和NY-ESO-1mRNA在GISTs中的高特异性表达,其抗原有望成为GISTs免疫治疗特异性的靶点;MAGE-1和NY-ESO-1mRNA表达与GISTs危险度分级有关,MAGE-1和NY-ESO-1mRNA有望成为辅助GISTs危险度分级的诊断指标.
基金This work was supported by a grant from the Tianjin Natural Science Foundation of China(No.023610811)
文摘OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PCR(real-time Q-PCR)assay. METHODS Peripheral blood samples were obtained from control subjects and 86 patients with hepatocellular carcinoma (HCC).Real-time Q-PCR was used to detect MAGE-1,MAGE-3, and AFP mRNAs in the blood cells. RESULTS In 86 tumor specimens,the positivity for MAGE-1, MAGE-3,and AFP genes was respectively 34.9%(30/86),60.5% (52/86)and 69.8%(60/86).All specimens expressed at least one marker.MAGE-1,MAGE-3,and AFP transcripts were detected respectively in 12(14.0%),18(20.1%)and 29(33.7%)of the 86 blood specimens from hepatocellular carcinoma patients,while 45 specimens(52.3%)were positive for at least one marker.In addition,MAGE-1,MAGE-3 and AFP gene transcripts were not detected in any peripheral blood specimens from 25 chronic liver disease patients and 28 normal healthy volunteers.The positive rate correlated with the TNM clinical stages,extrahepatic metastasis and portal vein carcinothrombosis(P<0.05).No correlation was found between tumor size,tumor number, differentiation,serum a-fetoprotein(AFP)and the positive rate. CONCLUSION Our results indicate that a multimarker real- time Q-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a hepatocyte-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.
