BACKGROUND Diabetic retinopathy(DR)is a major microvascular complication of diabetes mellitus,leading to significant visual impairment and blindness among adults.Current treatment options are limited,making it essenti...BACKGROUND Diabetic retinopathy(DR)is a major microvascular complication of diabetes mellitus,leading to significant visual impairment and blindness among adults.Current treatment options are limited,making it essential to explore novel therapeutic strategies.Curcumol,a sesquiterpenoid derived from traditional Chinese medicine,has shown anti-inflammatory and anti-cancer properties,but its potential role in DR remains unclear.AIM To investigate the therapeutic effects of curcumol on the progression of DR and to elucidate the underlying molecular mechanisms,particularly its impact on the fat mass and obesity-associated(FTO)protein and the long non-coding RNA(lncRNA)MAF transcription factor G antisense RNA 1(MAFG-AS1).METHODS A streptozotocin-induced mouse model of DR was established,followed by treatment with curcumol.Retinal damage and inflammation were evaluated through histological analysis and molecular assays.Human retinal vascular endothelial cells were exposed to high glucose conditions to simulate diabetic environments in vitro.Cell proliferation,migration,and inflammation markers were assessed in curcumoltreated cells.LncRNA microarray analysis identified key molecules regulated by curcumol,and further experiments were conducted to confirm the involvement of FTO and MAFG-AS1 in the progression of DR.RESULTS Curcumol treatment significantly reduced blood glucose levels and alleviated retinal damage in streptozotocininduced DR mouse models.In high-glucose-treated human retinal vascular endothelial cells,curcumol inhibited cell proliferation,migration,and inflammatory responses.LncRNA microarray analysis identified MAFG-AS1 as the most upregulated lncRNA following curcumol treatment.Mechanistically,FTO demethylated MAFG-AS1,stabilizing its expression.Rescue experiments demonstrated that the protective effects of curcumol against DR were mediated through the FTO/MAFG-AS1 signaling pathway.CONCLUSION Curcumol ameliorates the progression of DR by modulating the FTO/MAFG-AS1 axis,providing a novel therapeutic pathway for the treatment of DR.These findings suggest that curcumol-based therapies could offer a promising alternative for managing this debilitating complication of diabetes.展开更多
由于分布式电网中的非线性、不平衡负载和无功负载的增加,造成了谐波电流、负载不平衡电流、中性线电流和低功率因数等影响电能质量的问题,通常使用并联型有源电力滤波器来实现谐波消除、无功补偿、负载不平衡电流和中性线电流消除。在...由于分布式电网中的非线性、不平衡负载和无功负载的增加,造成了谐波电流、负载不平衡电流、中性线电流和低功率因数等影响电能质量的问题,通常使用并联型有源电力滤波器来实现谐波消除、无功补偿、负载不平衡电流和中性线电流消除。在有源电力滤波器的谐波检测方面,分析了传统基于瞬时无功功率理论方法的不足,提出了新的谐波检测方法,采用滑动平均滤波器(moving average filter,MAF)代替低通滤波器(low pass filter,LPF)进行直流分量的提取,克服了低通滤波器导致的传统检测方法实时性和动态性不强的缺陷,同时简化了模型结构。搭建了仿真与实验平台,仿真与实验结果表明新的谐波检测方法具有良好的检测效果和优越性。展开更多
文摘BACKGROUND Diabetic retinopathy(DR)is a major microvascular complication of diabetes mellitus,leading to significant visual impairment and blindness among adults.Current treatment options are limited,making it essential to explore novel therapeutic strategies.Curcumol,a sesquiterpenoid derived from traditional Chinese medicine,has shown anti-inflammatory and anti-cancer properties,but its potential role in DR remains unclear.AIM To investigate the therapeutic effects of curcumol on the progression of DR and to elucidate the underlying molecular mechanisms,particularly its impact on the fat mass and obesity-associated(FTO)protein and the long non-coding RNA(lncRNA)MAF transcription factor G antisense RNA 1(MAFG-AS1).METHODS A streptozotocin-induced mouse model of DR was established,followed by treatment with curcumol.Retinal damage and inflammation were evaluated through histological analysis and molecular assays.Human retinal vascular endothelial cells were exposed to high glucose conditions to simulate diabetic environments in vitro.Cell proliferation,migration,and inflammation markers were assessed in curcumoltreated cells.LncRNA microarray analysis identified key molecules regulated by curcumol,and further experiments were conducted to confirm the involvement of FTO and MAFG-AS1 in the progression of DR.RESULTS Curcumol treatment significantly reduced blood glucose levels and alleviated retinal damage in streptozotocininduced DR mouse models.In high-glucose-treated human retinal vascular endothelial cells,curcumol inhibited cell proliferation,migration,and inflammatory responses.LncRNA microarray analysis identified MAFG-AS1 as the most upregulated lncRNA following curcumol treatment.Mechanistically,FTO demethylated MAFG-AS1,stabilizing its expression.Rescue experiments demonstrated that the protective effects of curcumol against DR were mediated through the FTO/MAFG-AS1 signaling pathway.CONCLUSION Curcumol ameliorates the progression of DR by modulating the FTO/MAFG-AS1 axis,providing a novel therapeutic pathway for the treatment of DR.These findings suggest that curcumol-based therapies could offer a promising alternative for managing this debilitating complication of diabetes.
文摘由于分布式电网中的非线性、不平衡负载和无功负载的增加,造成了谐波电流、负载不平衡电流、中性线电流和低功率因数等影响电能质量的问题,通常使用并联型有源电力滤波器来实现谐波消除、无功补偿、负载不平衡电流和中性线电流消除。在有源电力滤波器的谐波检测方面,分析了传统基于瞬时无功功率理论方法的不足,提出了新的谐波检测方法,采用滑动平均滤波器(moving average filter,MAF)代替低通滤波器(low pass filter,LPF)进行直流分量的提取,克服了低通滤波器导致的传统检测方法实时性和动态性不强的缺陷,同时简化了模型结构。搭建了仿真与实验平台,仿真与实验结果表明新的谐波检测方法具有良好的检测效果和优越性。
