Nitrogen(N)serves both as a vital macronutrient and a signaling molecule for plants.Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism,which is essential for develop...Nitrogen(N)serves both as a vital macronutrient and a signaling molecule for plants.Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism,which is essential for developing maize with high N use efficiency.Two maize lines,B73 and Ki11,show differential chlorate and low-N tolerance.Time-course transcriptomic analysis reveals that the expression of N utilization genes(NUGs)in B73 and Ki11 have distinct responsive patterns to nitrate variation.By the coexpression networks,significant differences in the number of N response modules and regulatory networks of transcription factors(TFs)are revealed between B73 and Ki11.There are 23 unique TFs in B73 and 41 unique TFs in Ki11.MADS26 is a unique TF in the B73 N response network,with different expression levels and N response patterns in B73 and Ki11.Overexpression of MADS26 enhances the sensitivity to chlorate and the utilization of nitrate in maize,at least partially explaining the differential chlorate tolerance and low-N sensitivity between B73 and Ki11.The findings in this work provide unique insights and promising candidates for maize breeding to reduce unnecessary N overuse.展开更多
基金supported by the National Natural Science Foundation of China(32272133)the“JBGS”Project of Seed Industry Revitalization in Jiangsu Province(JBGS(2021)012)+2 种基金Jiangsu Agriculture Science and Technology Innovation Fund(CX(21)1003)Anhui Provincial Major Science and Technology Project(202003a06020004)Jiangsu Funding Program for Excellent Postdoctoral Talent(JB23058,2023ZB422).
文摘Nitrogen(N)serves both as a vital macronutrient and a signaling molecule for plants.Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism,which is essential for developing maize with high N use efficiency.Two maize lines,B73 and Ki11,show differential chlorate and low-N tolerance.Time-course transcriptomic analysis reveals that the expression of N utilization genes(NUGs)in B73 and Ki11 have distinct responsive patterns to nitrate variation.By the coexpression networks,significant differences in the number of N response modules and regulatory networks of transcription factors(TFs)are revealed between B73 and Ki11.There are 23 unique TFs in B73 and 41 unique TFs in Ki11.MADS26 is a unique TF in the B73 N response network,with different expression levels and N response patterns in B73 and Ki11.Overexpression of MADS26 enhances the sensitivity to chlorate and the utilization of nitrate in maize,at least partially explaining the differential chlorate tolerance and low-N sensitivity between B73 and Ki11.The findings in this work provide unique insights and promising candidates for maize breeding to reduce unnecessary N overuse.
