Anthocyanins are the main pigments in ripe strawberry fruits.FaMYB10 and abscisic acid(ABA)are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits.However,the transcript...Anthocyanins are the main pigments in ripe strawberry fruits.FaMYB10 and abscisic acid(ABA)are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits.However,the transcriptional regulation of FaMYB10,ABA biosynthesis,and anthocyanin accumulation in strawberry(Fragaria×ananassa)remain largely unknown.Here,a yeast one-hybrid screen using the FaMYB10 promoter identified a class B MADS-domain transcription factor,FaMADS6 in strawberry.FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit,a pattern opposite to accumulation of anthocyanins.Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation and expression of FaMYB10 and the anthocyanin biosynthetic genes,FaCHS,FaCHI,FaF3H,FaANS,and FaUFGT.In contrast,downregulation of FaMADS6 promoted the expression of FaMYB10 and the anthocyanin biosynthetic genes.The promoters of the anthocyanin biosynthetic genes were not directly bound by FaMADS6,in contrast to FaMYB10.Analysis of the DNA binding sequences of FaMADS6 revealed that it also interacted with the promoters of FaNCED2 and FaPYR1,which are involved in the biosynthesis and perception of ABA.Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry.Together,our findings suggest that FaMADS6 functions as a suppressor of anthocyanin accumulation by directly downregulating FaMYB10 and ABA production during strawberry fruit maturation.展开更多
植物MADS-box基因家族编码高度保守的转录因子,参与了包括花器官发育和开花在内的多种发育进程。为阐释兰科植物成花的分子调控机制,根据MADS-box基因保守序列设计简并引物,用RACE方法从朵丽蝶兰花葶中克隆到1个MADS-box家族基因,该基因...植物MADS-box基因家族编码高度保守的转录因子,参与了包括花器官发育和开花在内的多种发育进程。为阐释兰科植物成花的分子调控机制,根据MADS-box基因保守序列设计简并引物,用RACE方法从朵丽蝶兰花葶中克隆到1个MADS-box家族基因,该基因cDNA全长960 bp,包含37 bp 5'UTR,一个738 bp的开放阅读框(ORF)和185 bp 3'UTR,共编码245个氨基酸。序列和系统进化树分析表明,该基因与其他植物的MADS-box基因具有很高的同源性,属于AP1/FUL-like亚家族,命名为DtpsMADS1,GeneBank登录号为JQ065097。实时荧光定量PCR检测结果显示:DtpsMADS1具有明显的组织表达特异性;在根和叶中,DtpsMADS1在花前期和花后期表达量较高;苗期和盛花期表达量较低;DtpsMADS1在花葶中的表达趋势与根和叶相似;而在花器官中,DtpsMADS1只有痕量表达。由此推断,DtpsMADS1可能参与开花进程调控,而不参与花器官的形态建成。展开更多
基金supported by the National Key R&D Program of China(Grant No.2022YFD1600700)Natural Science Foundation of China(Grant Nos.32372644,32472661)+1 种基金Natural Science Foundation of Universities in Anhui Province,China(Grant Nos.2022AH050931,2023AH051043)Anhui Provincial Natural Science Foundation,China(Grant No.2108085MC105).
文摘Anthocyanins are the main pigments in ripe strawberry fruits.FaMYB10 and abscisic acid(ABA)are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits.However,the transcriptional regulation of FaMYB10,ABA biosynthesis,and anthocyanin accumulation in strawberry(Fragaria×ananassa)remain largely unknown.Here,a yeast one-hybrid screen using the FaMYB10 promoter identified a class B MADS-domain transcription factor,FaMADS6 in strawberry.FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit,a pattern opposite to accumulation of anthocyanins.Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation and expression of FaMYB10 and the anthocyanin biosynthetic genes,FaCHS,FaCHI,FaF3H,FaANS,and FaUFGT.In contrast,downregulation of FaMADS6 promoted the expression of FaMYB10 and the anthocyanin biosynthetic genes.The promoters of the anthocyanin biosynthetic genes were not directly bound by FaMADS6,in contrast to FaMYB10.Analysis of the DNA binding sequences of FaMADS6 revealed that it also interacted with the promoters of FaNCED2 and FaPYR1,which are involved in the biosynthesis and perception of ABA.Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry.Together,our findings suggest that FaMADS6 functions as a suppressor of anthocyanin accumulation by directly downregulating FaMYB10 and ABA production during strawberry fruit maturation.
文摘植物MADS-box基因家族编码高度保守的转录因子,参与了包括花器官发育和开花在内的多种发育进程。为阐释兰科植物成花的分子调控机制,根据MADS-box基因保守序列设计简并引物,用RACE方法从朵丽蝶兰花葶中克隆到1个MADS-box家族基因,该基因cDNA全长960 bp,包含37 bp 5'UTR,一个738 bp的开放阅读框(ORF)和185 bp 3'UTR,共编码245个氨基酸。序列和系统进化树分析表明,该基因与其他植物的MADS-box基因具有很高的同源性,属于AP1/FUL-like亚家族,命名为DtpsMADS1,GeneBank登录号为JQ065097。实时荧光定量PCR检测结果显示:DtpsMADS1具有明显的组织表达特异性;在根和叶中,DtpsMADS1在花前期和花后期表达量较高;苗期和盛花期表达量较低;DtpsMADS1在花葶中的表达趋势与根和叶相似;而在花器官中,DtpsMADS1只有痕量表达。由此推断,DtpsMADS1可能参与开花进程调控,而不参与花器官的形态建成。
