期刊文献+
共找到378篇文章
< 1 2 19 >
每页显示 20 50 100
The Research on the Stratagem of Feigning Madness Without Becoming Insane
1
作者 XING Cheng 《Psychology Research》 2025年第5期178-182,共5页
Feigning madness without becoming insane is a traditional Chinese stratagem recorded in Thirty-Six Stratagems,one of the most famous military works of ancient China.It can be seen in the military history and the polit... Feigning madness without becoming insane is a traditional Chinese stratagem recorded in Thirty-Six Stratagems,one of the most famous military works of ancient China.It can be seen in the military history and the political history of ancient China.It is a high-level psychological tactic,requiring the users to have extremely strong psychological resilience and great insight.Therefore,adopting the stratagem of feigning madness without becoming insane is the game of smart people.In brief,this stratagem is a kind of psychological defense tactic,helping the users protect themselves and gain enough time to prepare for the counterattack.About this stratagem,in ancient Chinese history,there were many successful examples.At the same time,failed cases are also worth studying.As a matter of fact,this traditional stratagem can be adopted in many different fields such as military,politics,and commerce.Thus,the research about this stratagem will be a good reference for the people of modern times. 展开更多
关键词 STRATAGEM psychological tactic feigning madness without becoming insane
在线阅读 下载PDF
A Deconstructive Reading of the Blurred Boundary between Madness and Reason in King Lear
2
作者 周慧娟 《海外英语》 2011年第10X期306-307,共2页
When normal, Lear can not even distinguish flattery and pat answers from sincerity and true love. While considered as insane, he learns to take care of the wretched and is able to discover the truth in life. The key c... When normal, Lear can not even distinguish flattery and pat answers from sincerity and true love. While considered as insane, he learns to take care of the wretched and is able to discover the truth in life. The key concept of anti-logo-centrism in deconstructive criticism provokes us to reconsider the boundary between madness and reason, and traditional Western philosophical ideas, thus enabling us to appreciate the despised madness in King Lear from the perspective of philosophy. 展开更多
关键词 DECONSTRUCTION anti-logo-centrism REASON madness
在线阅读 下载PDF
Tragic Experience and Critical Consciousness: Madness as the Presence of Transgression
3
作者 BAI Songzi QIN Mingli 《Journal of Philosophy Study》 2020年第4期252-256,共5页
The role of madness has been an unexcavated part in studies of Foucault on transgression,especially with literary studies.This paper considers madness as the presence of transgression,and gives an account of madness f... The role of madness has been an unexcavated part in studies of Foucault on transgression,especially with literary studies.This paper considers madness as the presence of transgression,and gives an account of madness from two different perspectives:tragic experience and critical consciousness.Madness as a tragic experience provides Foucault a way of transgressing death which is a limit that cannot be transcended;madness as a critical consciousness helps Foucault conceptualize the relation between reason and madness.The image of madmen in literary works can be recognized as the most typical embodiment of the connection between madness and transgression.Rather than aiming at a detailed explanation of Foucault’s understanding of madness,it attaches vital importance that how madness as a tragic experience and a critical consciousness becomes the presence of transgression. 展开更多
关键词 madness TRANSGRESSION TRAGIC EXPERIENCE CRITICAL CONSCIOUSNESS
暂未订购
The Feigned Madness of Ulysses and Hamlet: A Derridean Reading of Cartesian Cogito
4
作者 Dina Mohamed Abd Elsalam 《Journal of Literature and Art Studies》 2016年第2期135-146,共12页
Ulysses, the towering mythological figure and the hero of the world's most famous epic The Odyssey, put on the pretence of madness to shirk the Trojan War. This is not mentioned in Homer, but in Fabulae by Gaius Juli... Ulysses, the towering mythological figure and the hero of the world's most famous epic The Odyssey, put on the pretence of madness to shirk the Trojan War. This is not mentioned in Homer, but in Fabulae by Gaius Julius Hyginus. Similarly, Hamlet put on an "antic disposition" after the ghost exhorted him to kill King Claudius. In these two cases taken from mythology and literature, and pertaining to the Classical period and the Elizabethan age respectively, intelligent characters put on the pretence of madness, in their battle with society though they are in control of their senses. It is the aim of this paper to examine the dynamics of reason and non-reason when combined and brought so close to one another, that they could be easily confounded, in light of Derrida's reading of Descartes' formulations about reason. Since the two examined works pertain to the Classical and Elizabethan ages respectively, the paper will shed light on the historical background of madness in these periods to give a broader perspective of reason and madness in these works. 展开更多
关键词 feigned madness reason ULYSSES HAMLET DERRIDA DESCARTES Gaius Julius Hyginus Shakespeare
在线阅读 下载PDF
Illness in the BrontE Sisters' Novels Alcoholism, Madness, and Civilization
5
作者 Ioana Boghian 《Sino-US English Teaching》 2013年第8期638-649,共12页
Images of illness and disease, for example, cholera, consumption, rabies, rheumatism, fevers, alcoholism, hypochondria, hysteria, monomania, and madness, are present in all the seven novels written by the Bronte siste... Images of illness and disease, for example, cholera, consumption, rabies, rheumatism, fevers, alcoholism, hypochondria, hysteria, monomania, and madness, are present in all the seven novels written by the Bronte sisters during the years of 1847-1853. The lack of cures for most of these diseases which often led to death during the respective period seems to account for such literary representations of poor bodily conditions. The purpose of this paper is, however, to go beyond this handy and obvious explanation regarding illness in the BrontE sisters' novels and approach aspects such as: Victorian relationships between women and men, the condition of Victorian women in terms of social and domestic status, positions and roles, and rights and education in a patriarchal age. The novels that shall constitute the support for our analysis are The Tenant of Wildfell Hall (2001) by Anne Bronte, Jane Eyre (2001) by CharIotte Bronte, and Wuthering Heights (1985) by Emily Bronte. 展开更多
关键词 illness ALCOHOLISM madness CIVILIZATION patriarchal system
在线阅读 下载PDF
Marriage in A Midsummer Night's Dream: A Return From Madness to Reason
6
作者 康杰 《海外英语》 2017年第3期172-174,共3页
Shakespeare’s A Midsummer Night’s Dream is a play of love in which Shakespeare depicts a vivid picture of the madness and irrationality in romantic love.Yet Shakespeare entitles this play"a dream"and concl... Shakespeare’s A Midsummer Night’s Dream is a play of love in which Shakespeare depicts a vivid picture of the madness and irrationality in romantic love.Yet Shakespeare entitles this play"a dream"and concludes the play with a happy ending of marriage for all lovers.Shakespeare thus clearly views romantic love as an inevitable but short episode of life,an adolescent dream that should finally come to an end with the lovers’marriage which signifies their attainment of maturity and brings them back to reason and reality. 展开更多
关键词 SHAKESPEARE love DREAM madness REASON
在线阅读 下载PDF
There’s Method in His Madness
7
作者 Ni Yanshuo 《ChinAfrica》 2011年第8期18-19,共2页
Big-hearted Chinese entrepreneur cherishes the spotlight HE isn’t as widely known as philanthropist heavyweights Bill Gates and Warren Buffett, but Chinese tycoon Chen Guangbiao is no stranger to publicity. Bucking t... Big-hearted Chinese entrepreneur cherishes the spotlight HE isn’t as widely known as philanthropist heavyweights Bill Gates and Warren Buffett, but Chinese tycoon Chen Guangbiao is no stranger to publicity. Bucking the Chinese tradition of remaining low-key when making donations, Chen widely publicizes every 展开更多
关键词 There’s Method in His madness
原文传递
新型一期给药方案与传统三期给药方案对骨折愈合的疗效及机制研究
8
作者 王琳珏 古恩鹏 +1 位作者 郭锐 王佳乐 《中国中西医结合外科杂志》 2026年第1期24-29,共6页
目的:比较新型一期给药方案与传统三期给药方案对骨折愈合的促进作用,并初步探究其对骨形态发生蛋白(BMP)/Sma和Mad相关蛋白(Smad)通路的激活效应。方法:选取24只雄性SD大鼠,随机分为空白对照组、模型对照组、一期给药组(全程予综合方)... 目的:比较新型一期给药方案与传统三期给药方案对骨折愈合的促进作用,并初步探究其对骨形态发生蛋白(BMP)/Sma和Mad相关蛋白(Smad)通路的激活效应。方法:选取24只雄性SD大鼠,随机分为空白对照组、模型对照组、一期给药组(全程予综合方)和三期给药组(1~7 d予复元活血汤,8~28 d予和营止痛汤,28 d后予健步虎潜丸)。术后行X射线评估大鼠骨折愈合程度;测定(Ca)、磷(P)、碱性磷酸酶(ALP)及酸性磷酸酶(ACP)水平;苏木精-伊红(HE)染色观察骨痂组织愈合情况;免疫组化检测BMP2、Runt相关转录因子(Runx2)、Smad1蛋白水平;RT-qPCR检测BMP2、Runx2、Smad1的mRNA表达水平。结果:与模型对照组相比,一期给药组和三期给药组的骨折愈合进程均明显加快,一期给药组尤为显著。模型对照组的ALP为(165±4.7)U/L,一期给药组和三期给药组分别为(233±12.2)U/L和(174±20.3)U/L,ALP在一期给药组中显著升高,提示其加速骨折修复显著优于模型对照组和三期给药组。一期给药组的ACP水平为(0.192±0.027),显著低于模型对照组的(0.340±0.017)和三期给药组的(0.265±0.014)。HE染色显示,一期给药组的局部炎症浸润明显减少,软骨及新骨形成丰富;三期给药组亦见炎症减轻及胶原/软骨组织增多。免疫组化表明,一期给药组的Runx2表达水平为(57.1±5.9)%,显著高于模型对照组的(41.6±3.1)%和三期给药组的(48.3±4.0)%,Smad1和BMP2亦有不同程度的升高,RT-qPCR检测结果与上述免疫组化结果一致。结论:一期给药方案通过激活BMP/Smad信号通路有效促进骨折成骨与重塑,其效果优于三期给药方案。 展开更多
关键词 骨折 中药复方 骨形态发生蛋白 Sma和Mad相关蛋白 一期给药 三期给药
暂未订购
FaMADS6 negatively regulates anthocyanin accumulation in strawberry by inhibiting FaMYB10 expression and ABA biosynthesis
9
作者 Yang Zhang Shuaishuai Wang +9 位作者 Xiaoshan Wang Ting Qian Yanan Sun Mauren Jaudal Ross GAtkinson Jing Zhao Guanghui Zheng Peipei Sun Congbing Fang Xingbin Xie 《Horticultural Plant Journal》 2026年第2期300-316,共17页
Anthocyanins are the main pigments in ripe strawberry fruits.FaMYB10 and abscisic acid(ABA)are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits.However,the transcript... Anthocyanins are the main pigments in ripe strawberry fruits.FaMYB10 and abscisic acid(ABA)are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits.However,the transcriptional regulation of FaMYB10,ABA biosynthesis,and anthocyanin accumulation in strawberry(Fragaria×ananassa)remain largely unknown.Here,a yeast one-hybrid screen using the FaMYB10 promoter identified a class B MADS-domain transcription factor,FaMADS6 in strawberry.FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit,a pattern opposite to accumulation of anthocyanins.Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation and expression of FaMYB10 and the anthocyanin biosynthetic genes,FaCHS,FaCHI,FaF3H,FaANS,and FaUFGT.In contrast,downregulation of FaMADS6 promoted the expression of FaMYB10 and the anthocyanin biosynthetic genes.The promoters of the anthocyanin biosynthetic genes were not directly bound by FaMADS6,in contrast to FaMYB10.Analysis of the DNA binding sequences of FaMADS6 revealed that it also interacted with the promoters of FaNCED2 and FaPYR1,which are involved in the biosynthesis and perception of ABA.Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry.Together,our findings suggest that FaMADS6 functions as a suppressor of anthocyanin accumulation by directly downregulating FaMYB10 and ABA production during strawberry fruit maturation. 展开更多
关键词 Fragaria×ananassa Duch. ANTHOCYANIN ABA REPRESSOR MADS Transcriptional regulation
在线阅读 下载PDF
MICHELIN MADNESS What Four Guangzhou Chefs ReallyThink of the 2018 Michelin Guide
10
作者 Matthew Bossons 《城市漫步(GBA版)》 2018年第8期51-51,共1页
The first-ever Guangzhou version of the esteemed Michelin Guide has arived,and people-particularly those in the F&B industry-have opinions.
关键词 GUANGZHOU MAD OPINION
原文传递
MOBILE MADNESS 5 Depressing Stats About Chinese Mobile Phone Users
11
作者 Ryan Gandolfo 《城市漫步(上海版、英文)》 2019年第7期7-7,共1页
Anew survey has come out that reveals Chinese people are seriously addicted to their phones.Surprised?We didn't think so.Reports have surfaced in recent years showing a disturbing trend in how much peo・pie use the... Anew survey has come out that reveals Chinese people are seriously addicted to their phones.Surprised?We didn't think so.Reports have surfaced in recent years showing a disturbing trend in how much peo・pie use their phones on a daily basis,and it doesn't seem to be improving anytime soon.China Youth Dailys survey center wenjuan. 展开更多
关键词 MAD seriously SURVEY
原文传递
转录因子对园艺作物果肉颜色的调控 被引量:1
12
作者 袁路明 朱红菊 刘文革 《园艺学报》 北大核心 2025年第4期1051-1064,共14页
果肉颜色是园艺作物果实的重要性状,对自身种子传播、营养物质评价,消费者选择都十分重要。果肉颜色表现出多样性及复杂性,比如猕猴桃果肉存在绿、黄、红心,西瓜果肉存在黄、红、白、混合等多种颜色。果肉颜色的形成主要受色素类物质的... 果肉颜色是园艺作物果实的重要性状,对自身种子传播、营养物质评价,消费者选择都十分重要。果肉颜色表现出多样性及复杂性,比如猕猴桃果肉存在绿、黄、红心,西瓜果肉存在黄、红、白、混合等多种颜色。果肉颜色的形成主要受色素类物质的组成及含量影响,如类胡萝卜素、叶绿素、花青素苷等。通过调控色素类物质代谢通路中的关键基因表达,可以实现对园艺产品颜色的调控。转录因子(Transcription factor,TF)通过与相关基因的启动子区域结合,激活或抑制基因的表达,实现对基因表达和颜色的调控。在前人的研究中,多种转录因子被发掘并证明对色素代谢通路中相关基因及颜色具有重要调控作用,如MYB、NAC、MADS、WRKY等。 展开更多
关键词 转录因子 果肉颜色 色素调控 MYB NAC MADS WRKY
原文传递
滇水金凤花距发育相关基因MADS-EJ2和MADS2的克隆及表达分析 被引量:1
13
作者 王天野 杨梦情 +3 位作者 马慧 梁光容 黄美娟 黄海泉 《山东农业科学》 北大核心 2025年第9期1-8,共8页
E类MADS-box转录因子基因SEPALLATA(SEP)-like在花器官发育方面发挥着重要作用。为了探究基因SEP-like在滇水金凤(Impatiens uliginosa)花距发育中的功能,本研究成功克隆出基因MADS-EJ2和MADS2,分别命名为IuMADS-EJ2和IuMADS2,其cDNA全... E类MADS-box转录因子基因SEPALLATA(SEP)-like在花器官发育方面发挥着重要作用。为了探究基因SEP-like在滇水金凤(Impatiens uliginosa)花距发育中的功能,本研究成功克隆出基因MADS-EJ2和MADS2,分别命名为IuMADS-EJ2和IuMADS2,其cDNA全长均为762 bp,编码253 aa。生物信息学分析表明,IuMADS-EJ2为亲水性稳定蛋白,IuMADS2为亲水性不稳定蛋白,二者均含MIKC结构,在C-端都有其亚家族特异的SEPⅠ和SEPⅡ基序,均为E类基因;亚细胞定位预测IuMADS-EJ2和IuMADS2均定位在细胞核。IuMADS-EJ2和IuMADS2的氨基酸序列与喜马拉雅凤仙花的同源序列相似性分别为98.03%和95.26%。qRT-PCR分析表明,两基因在滇水金凤花距发育的3个时期及2个不同部位均有表达,IuMADS-EJ2在花苞期距部的表达量最高,IuMADS2在花苞期檐部的表达量最高,推测两基因调控花距发育且主要在花距发育初期发挥作用。本研究结果可为凤仙花花距发育分子机制解析、花形改良和新品种培育提供一定的基础数据和理论依据。 展开更多
关键词 滇水金凤 花距发育 MADS-EJ2基因 MADS2基因 生物信息学分析 表达分析
在线阅读 下载PDF
利拉鲁肽改善非酒精性脂肪性肝病小鼠肝纤维化的作用及其机制
14
作者 王仁杰 祝超瑜 +4 位作者 方云云 肖元元 王倩倩 宋雯婧 魏丽 《上海交通大学学报(医学版)》 北大核心 2025年第4期415-425,共11页
目的·探讨利拉鲁肽对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)小鼠肝纤维化的作用及其机制。方法·将20只8周龄C57BL/6J小鼠随机分为正常饮食组(Chow组)及甲硫氨酸-胆碱缺乏(methionine-choline deficien... 目的·探讨利拉鲁肽对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)小鼠肝纤维化的作用及其机制。方法·将20只8周龄C57BL/6J小鼠随机分为正常饮食组(Chow组)及甲硫氨酸-胆碱缺乏(methionine-choline deficient,MCD)饮食喂养组(MCD组),每组10只。采用MCD饮食诱导构建NAFLD小鼠。将2组小鼠随机分为4个亚组,分别为Chow+生理盐水组、Chow+利拉鲁肽组、MCD+生理盐水组、MCD+利拉鲁肽组。每日分别腹腔注射利拉鲁肽(400μg/kg)或同体积生理盐水4周后,行腹腔注射葡萄糖耐量实验(intraperitoneal glucose tolerance test,IPGTT)。测定小鼠血清天冬氨酸转氨酶(aspartate transaminase,AST)、丙氨酸转氨酶(alanine aminotransferase,ALT)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TAG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDLC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)水平。小鼠处死后取其肝组织测定TAG含量,对肝组织行苏木精-伊红(hematoxylin-eosin,HE)染色、油红O染色及马松(Masson)染色观察肝脏病理结构、脂质沉积和纤维化程度。采用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,qPCR)与Western blotting检测肝组织α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、纤连蛋白(fibronectin,FN)、Ⅰ型胶原蛋白α(collagen typeⅠα,COL1A)、基质金属蛋白酶9(matrix metallo protein 9,MMP9)、金属蛋白酶组织抑制因子1(tissue inhibitor of metal protease 1,TIMP1)和转化生长因子β(transforming growth factor-β,TGF-β)表达水平,检测Sma和Mad相关蛋白3(Sma-and Mad-related protein 3,SMAD3)及磷酸化SMAD3(SMAD3 phosphorylation,pSMAD3)蛋白表达水平。结果·利拉鲁肽干预后,IPGTT显示小鼠血糖在第15、30、60 min时均有所下降,受试者操作特征曲线(receiver operator characteristic curve,ROC曲线)的曲线下面积(area under the curve,AUC)降低(均P<0.05)。血清生化检测结果显示,利拉鲁肽干预后,MCD组小鼠AST与ALT水平降低(均P<0.001),TC、HDL-C水平升高(均P<0.05),TAG、LDL-C无明显变化。HE染色与油红O染色显示,利拉鲁肽干预后,肝细胞脂滴数量明显减少,气球样变性和炎症浸润程度减轻。Masson染色显示,利拉鲁肽干预后,MCD组小鼠肝脏蓝色胶原纤维数量明显减少。qPCR与Western blotting检测显示,MCD组小鼠肝脏α-SMA、FN、COL1A、TIMP1、TGF-β、pSMAD3/SMAD3表达明显上调,MMP9蛋白表达下降;经利拉鲁肽干预后,MCD组小鼠肝脏α-SMA、FN、COL1A、TIMP1、TGF-β、pSMAD3/SMAD3表达明显降低,MMP9表达上升。结论·利拉鲁肽能缓解NAFLD小鼠肝损伤、肝脏脂质沉积及纤维化程度,并通过调控TGF-β/SMAD3通路和纤维化相关蛋白表达改善小鼠肝功能和肝纤维化。 展开更多
关键词 利拉鲁肽 转化生长因子Β Sma和Mad相关蛋白3 非酒精性脂肪性肝病 肝纤维化
暂未订购
融入中医情志相胜法的睡眠集束化模式睡眠管理对肝癌TACE术后失眠病人的影响 被引量:1
15
作者 张敏敏 袁晓利 王欣 《循证护理》 2025年第15期3143-3147,共5页
目的:探究融入中医情志相胜法的睡眠集束化(SLEEP⁃MAD)模式睡眠管理对肝癌动脉化疗栓塞术(TACE)术后失眠病人的影响。方法:选取2022年4月—2024年3月于我院行肝癌TACE术治疗并在术后出现失眠症状的151例病人作为研究对象,采用随机数字... 目的:探究融入中医情志相胜法的睡眠集束化(SLEEP⁃MAD)模式睡眠管理对肝癌动脉化疗栓塞术(TACE)术后失眠病人的影响。方法:选取2022年4月—2024年3月于我院行肝癌TACE术治疗并在术后出现失眠症状的151例病人作为研究对象,采用随机数字表法将其分为研究组(n=76)和对照组(n=75)。对照组实施常规护理,研究组在对照组基础上实施融入中医情志相胜法的SLEEP⁃MAD模式睡眠管理,两组均干预7 d。比较两组病人干预前后负性情绪、睡眠质量和生活质量。结果:干预后,研究组焦虑和抑郁维度评分低于对照组,研究组匹兹堡睡眠质量指数量表各维度评分及总分均低于对照组,研究组总睡眠时间高于对照组,研究组觉醒次数、觉醒时间和>5 min觉醒次数均低于对照组,差异均有统计学意义(P<0.001)。结论:对肝癌TACE术后失眠病人实施融入中医情志相胜法的SLEEP⁃MAD模式睡眠管理有助于改善病人负性情绪,提高病人的睡眠质量和睡眠情况。 展开更多
关键词 中医情志相胜法 SLEEP⁃MAD模式 肝癌 动脉化疗栓塞术 失眠 负性情绪 睡眠质量 护理
暂未订购
微小RNA-155-5p靶向沉默信息调节因子1调节转化生长因子-β/Sma和Mad相关蛋白信号通路对糖尿病肾脏疾病大鼠肾纤维化作用机制的研究 被引量:3
16
作者 廖青 王小平 《中国糖尿病杂志》 北大核心 2025年第1期57-64,共8页
目的 研究miR-155-5p靶向沉默信息调节因子1(SIRT1)调节TGF-β/Sma和Mad相关蛋白(TGF-β/Smad)信号通路,对DKD大鼠肾纤维化的影响机制。方法 48只大鼠随机分为正常对照(NC)组、模型(Mod)组、抑制剂阴性对照(anti-NC)组、miR-155-5p抑制... 目的 研究miR-155-5p靶向沉默信息调节因子1(SIRT1)调节TGF-β/Sma和Mad相关蛋白(TGF-β/Smad)信号通路,对DKD大鼠肾纤维化的影响机制。方法 48只大鼠随机分为正常对照(NC)组、模型(Mod)组、抑制剂阴性对照(anti-NC)组、miR-155-5p抑制剂(anti-miR-155-5p)组、miR-155-5p抑制剂+小分子干扰RNA阴性对照(anti-miR-155-5p+si-NC)组、miR-155-5p抑制剂+SIRT1小分子干扰RNA(anti-miR-155-5p+si-SIRT1)组,每组8只。检测各组FPG、24 h UAlb、BUN、血肌酐(Scr)。HE、Masson染色比较各组肾组织病理变化,计算胶原容积分数(CVF)。RT-qRCR检测miR-155-5p、SIRT1 mRNA表达,Western blot法检测各组SIRT1、TGF-β1、Smad3、Smad7、结缔组织生长因子(CTGF)、Ⅰ型胶原蛋白(COLⅠ)蛋白表达。结果 与NC组比较,Mod、anti-NC组FPG、24 hUAlb、BUN、Scr、CVF、miR-155-5p表达及TGF-β1、Smad3、CTGF、COLⅠ蛋白表达升高,SIRT1 mRNA和蛋白表达、Smad7蛋白表达降低(P<0.05)。与anti-NC组比较,anti-miR-155-5p、anti-miR-155-5p+si-NC组SIRT1 mRNA和蛋白表达、Smad7蛋白表达升高(P<0.05),FPG、24 hUAlb、BUN、Scr、CVF、miR-155-5p表达及TGF-β1、Smad3、CTGF、COLⅠ蛋白表达降低(P<0.05)。与anti-miR-155-5p、anti-miR-155-5p+si-NC组比较,anti-miR-155-5p+si-SIRT1组FPG、24h UAlb、BUN、Scr、CVF、miR-155-5p表达及TGF-β1、Smad3、CTGF、COLⅠ蛋白表达升高(P<0.05),SIRT1 mRNA和蛋白表达、Smad7蛋白表达降低(P<0.05)。SIRT1 mRNA的3’UTR含有miR-155-5p序列保守碱基。结论 DKD大鼠中升高miR-155-5p可靶向调控SIRT1,改善肾纤维化。 展开更多
关键词 微小RNA-155-5p 沉默信息调节因子1 转化生长因子-β/Sma和Mad相关蛋白信号通路 糖尿病肾脏疾病 肾纤维化
原文传递
薄壳山核桃MADS基因家族的全基因组鉴定及生物信息学分析
17
作者 方欣妍 何秀琼 +3 位作者 黄丹 彭兵阳 孙佳雯 吕佳斌 《江苏农业学报》 北大核心 2025年第6期1080-1094,共15页
MADS基因是一类重要的转录调控因子,具有广泛的生物学功能,参与多个物种的生长发育和信号传导,在多个物种中的生物学特性已被鉴定。本研究通过生物信息学方法对薄壳山核桃的MADS基因家族成员进行鉴定分析,最终鉴定出81个MADS基因家族成... MADS基因是一类重要的转录调控因子,具有广泛的生物学功能,参与多个物种的生长发育和信号传导,在多个物种中的生物学特性已被鉴定。本研究通过生物信息学方法对薄壳山核桃的MADS基因家族成员进行鉴定分析,最终鉴定出81个MADS基因家族成员,不均匀分布在16条染色体上,根据系统进化树分析结果MADS蛋白分为type-Ⅰ型(20个)和type-Ⅱ型(61个)。薄壳山核桃该家族基因具有较多顺式作用元件,植物激素响应元件、逆境胁迫响应元件和光响应元件等,研究发现这些作用元件广泛存在于MADS基因的启动子区域,表明MADS基因在薄壳山核桃适应环境变化及生长发育过程中充当重要角色。不同薄壳山核桃MADS家族基因在不同组织中和不同时期的表达量差异较大,如CiMADS46在茎、CiMADS7在果实具有较高表达量,CiMADS11的表达量随着时间推移递减。MADS基因具有不同的组织特异性表达模式,推测MADS基因家族可能在成花及果实发育过程中发挥重要作用,在不同发育阶段具有一定程度的功能多样性。本研究结果为进一步研究CiMADS基因功能与特性奠定了基础,并为其他物种的MADS基因家族成员的鉴定和表征提供参考。 展开更多
关键词 薄壳山核桃 MADS基因家族 生物信息学 表达分析
在线阅读 下载PDF
浅地层剖面上规律性声脉冲噪声的压制:一种基于MAD的开关中值滤波方法
18
作者 肖壹宝 李西双 +3 位作者 刘乐军 马龙 刘晨光 郭若舜 《海洋学报》 北大核心 2025年第6期119-128,共10页
海上综合工程物探调查过程中,当缺少声学同步器时,多波束等其他声学设备发出的声脉冲对于浅地层剖面测量来说是一种噪声,作为一种干扰波会严重影响浅地层剖面资料的信噪比与分辨率。此类噪声在频率上与有效信号相近,振幅强且可能出现于... 海上综合工程物探调查过程中,当缺少声学同步器时,多波束等其他声学设备发出的声脉冲对于浅地层剖面测量来说是一种噪声,作为一种干扰波会严重影响浅地层剖面资料的信噪比与分辨率。此类噪声在频率上与有效信号相近,振幅强且可能出现于地震记录的任何位置。尽管现有的中值滤波可以对此类干扰波进行压制,但也在一定程度上损害了有效信号。本文提出了一种改进的开关中值滤波方法,该方法首先基于MAD准则来判断浅地层剖面中的干扰波的位置,然后又使用中值滤波方法对干扰波附近的信号进行去噪处理,不含噪声的信号则不进行处理。利用该方法对南黄海陆架获得浅地层剖面进行处理,结果显示,与以往的方法相比,本文提出的改进的开关中值滤波法不但可以有效地衰减与多波束声脉冲类似的声学干扰波,而且尽可能地避免了有效信号的损失。 展开更多
关键词 浅地层剖面 多波束声脉冲 MAD 开关中值滤波
在线阅读 下载PDF
Time-course transcriptomic analysis reveals transcription factors involved in modulating nitrogen sensibility in maize
19
作者 Mingliang Zhang Yuancong Wang +9 位作者 Qi Wu Yangming Sun Chenxu Zhao Min Ge Ling Zhou Tifu Zhang Wei Zhang Yiliang Qian Long Ruan Han Zhao 《Journal of Genetics and Genomics》 2025年第3期400-410,共11页
Nitrogen(N)serves both as a vital macronutrient and a signaling molecule for plants.Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism,which is essential for develop... Nitrogen(N)serves both as a vital macronutrient and a signaling molecule for plants.Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism,which is essential for developing maize with high N use efficiency.Two maize lines,B73 and Ki11,show differential chlorate and low-N tolerance.Time-course transcriptomic analysis reveals that the expression of N utilization genes(NUGs)in B73 and Ki11 have distinct responsive patterns to nitrate variation.By the coexpression networks,significant differences in the number of N response modules and regulatory networks of transcription factors(TFs)are revealed between B73 and Ki11.There are 23 unique TFs in B73 and 41 unique TFs in Ki11.MADS26 is a unique TF in the B73 N response network,with different expression levels and N response patterns in B73 and Ki11.Overexpression of MADS26 enhances the sensitivity to chlorate and the utilization of nitrate in maize,at least partially explaining the differential chlorate tolerance and low-N sensitivity between B73 and Ki11.The findings in this work provide unique insights and promising candidates for maize breeding to reduce unnecessary N overuse. 展开更多
关键词 MAIZE GENOTYPE N response DiffCoEx network MADS26
原文传递
金钗石斛花发育和胁迫的转录模式及MADS家族表达分析
20
作者 陈宏宇 于莹 桑思宏 《安徽农学通报》 2025年第20期104-109,共6页
本研究以金钗石斛花为试验材料,利用转录组学对该植物的3个发育阶段[小花苞期(Ⅰ期)、大花苞期(Ⅱ期)、开放期(Ⅲ期)]和3种胁迫(低温、高温和机械损伤,均以Ⅲ期样本为材料)的样本进行测序,分析其发育(Ⅰ期vsⅡ期、Ⅱ期vsⅢ期和Ⅰ期vsⅢ... 本研究以金钗石斛花为试验材料,利用转录组学对该植物的3个发育阶段[小花苞期(Ⅰ期)、大花苞期(Ⅱ期)、开放期(Ⅲ期)]和3种胁迫(低温、高温和机械损伤,均以Ⅲ期样本为材料)的样本进行测序,分析其发育(Ⅰ期vsⅡ期、Ⅱ期vsⅢ期和Ⅰ期vsⅢ期)和胁迫(Ⅲ期胁迫vsⅢ期)的转录模式及MADS转录因子的差异表达特征。结果表明,Ⅰ期vsⅡ期、Ⅱ期vsⅢ期和Ⅰ期vsⅢ期、低温、高温和机械损伤分别识别了26 598、29 680、30 263、7 495、13 713和8 764个差异表达基因。GO功能聚类表明,差异表达基因主要聚集在生物过程、细胞组分和分子功能;KEGG调控通路分析表明,差异表达基因主要与植物激素信号转导、氨基糖和核苷酸糖代谢、植物病原体相互作用及植物MAPK信号通路等过程有关。金钗石斛花不同发育阶段和胁迫下差异表达的MADS转录因子共有74个,Ⅰ期vsⅡ期、Ⅱ期vsⅢ期和Ⅰ期vsⅢ期、低温、高温、机械损伤分别有40、34、40、17、25、16个。研究结果为解析金钗石斛花发育和胁迫应答分子机制提供参考。 展开更多
关键词 金钗石斛花 转录模式 发育 胁迫 MADS家族
在线阅读 下载PDF
上一页 1 2 19 下一页 到第
使用帮助 返回顶部