Soil salinization and alkalization have become an increasingly severe global issues,significantly limiting both the yield and quality of apples(Malus×domestica).M9-T337 is a widely used apple dwarfing rootstock;h...Soil salinization and alkalization have become an increasingly severe global issues,significantly limiting both the yield and quality of apples(Malus×domestica).M9-T337 is a widely used apple dwarfing rootstock;however,it is sensitive to saline-alkali stress.Therefore,developing saline-alkali tolerant apple rootstocks is essential.In this study,we utilized RNAi(RNA interference)technology to knock down GH3 genes in the M9-T337 background,aiming to engineer a dwarfing and stress-tolerant apple rootstock.We found that MdGH3 RNAi plants exhibited superior morphology compared to M9-T337 under saline-alkali stress conditions,characterized by more robust root systems,increased plant height,a lower Na^(+)/K^(+)ratio,and enhanced photosynthetic and antioxidant capacities.Moreover,when MdGH3 RNAi plants were used as rootstocks,the GL-3/MdGH3 RNAi plants also displayed greater plant height,root vitality,photosynthetic ability,and antioxidant capacity compared to GL-3 grafted onto M9-T337 rootstock.Taken together,our study constructed a saline-alkali-tolerant apple rootstock by knocking down MdGH3 genes.展开更多
基金supported by the National Natural Science Foundation of China(32202459,32441069)the Key S&T Special Projects of Xinjiang,China(2023B02018-2)+1 种基金Xinjiang Apple Research System of China(XJLGCYJSTX04)Chinese Universities Scientific Fund(2452023067).
文摘Soil salinization and alkalization have become an increasingly severe global issues,significantly limiting both the yield and quality of apples(Malus×domestica).M9-T337 is a widely used apple dwarfing rootstock;however,it is sensitive to saline-alkali stress.Therefore,developing saline-alkali tolerant apple rootstocks is essential.In this study,we utilized RNAi(RNA interference)technology to knock down GH3 genes in the M9-T337 background,aiming to engineer a dwarfing and stress-tolerant apple rootstock.We found that MdGH3 RNAi plants exhibited superior morphology compared to M9-T337 under saline-alkali stress conditions,characterized by more robust root systems,increased plant height,a lower Na^(+)/K^(+)ratio,and enhanced photosynthetic and antioxidant capacities.Moreover,when MdGH3 RNAi plants were used as rootstocks,the GL-3/MdGH3 RNAi plants also displayed greater plant height,root vitality,photosynthetic ability,and antioxidant capacity compared to GL-3 grafted onto M9-T337 rootstock.Taken together,our study constructed a saline-alkali-tolerant apple rootstock by knocking down MdGH3 genes.
