AIM: To explore the value of serum M2-pyruvate kinase (M2-PK) in colorectal cancer (CRC) mass screening. METHODS: We conducted a molecular epidemiology study in Hangzhou, China, from year 2006 to year 2008. Serum samp...AIM: To explore the value of serum M2-pyruvate kinase (M2-PK) in colorectal cancer (CRC) mass screening. METHODS: We conducted a molecular epidemiology study in Hangzhou, China, from year 2006 to year 2008. Serum samples were collected from 93 CRC, 41 advanced adenomas, 137 adenomas, 47 non-adenomatous polyps, and 158 normal participants in a community setting. Serum M2-PK and carcinoembryonic antigen (CEA) were measured using Enzyme-linked immunosorbent assay. SPSS 16.0 software was used to perform data analysis. Area under the receiver operating characteristic curve (AUC), sensitivity, and specificities were estimated for serum M2-PK in diagnosis of colorectal lesions and compared with CEA. RESULTS: Average serum M2-PK value among 158 normal people was 2.96 U/mL and not affected by gender (P = 0.47) or age (P = 0.59). Average serum M2-PK (U/mL) was 14.75 among stage III and 13.10 among stage?I?and II CRC patients, about 4 times higher than that among normal people. Average serum M2-PK was 8.58, 6.70, 5.13 and 2.51 U/mL among advanced adenoma, adenomas, non-adenomatous polyps, and inflammatory bowel disease patients, respectively. AUC for serum M2-PK was greater than that for CEA among all colorectal lesions. AUC for serum M2-PK was 0.89 (0.84, 0.94) (95% confidence interval), higher than that for CEA [0.70 (0.62-0.79)] in CRC stage?I?and II, 0.89 (0.84-0.94) vs 0.73 (0.63-0.83) in CRC stage III, 0.81 (0.74-0.86) vs 0.63 (0.53 - 0.73) in advanced adenomas, 0.69 (0.64-0.76) vs 0.54 (0.47-0.60) in adenomas, and 0.69 (0.62-0.78) vs 0.58 (0.48-0.68) in non-adenomatous polyps. The diagnostic sensitivity for all colorectal lesions increased with decrease in the cut-off value of serum M2-PK. The diagnostic sensitivity (%) of serum M2-PK was 100.00 for CRC, 95.12 advanced adenoma, 82.48 adenoma, and 82.98 non-adenomatous polyp. There were no CRC cases missed and 40.51% of unnecessary colonoscopies were avoided when the cut-off value was 2.00 U/mL. CONCLUSION: Serum M2-PK can be used as a primary screening test in CRC mass screening. It may be a promising non-invasive biomarker for CRC early detection.展开更多
2022年中央一号文件《中共中央国务院关于做好2022年全面推进乡村振兴重点工作的意见》明确要求大力推进数字农业建设,推进智慧农业发展;2022年12月23日至24日中央农村工作会议上习近平总书记指出“产业振兴是乡村振兴的重中之重,要落...2022年中央一号文件《中共中央国务院关于做好2022年全面推进乡村振兴重点工作的意见》明确要求大力推进数字农业建设,推进智慧农业发展;2022年12月23日至24日中央农村工作会议上习近平总书记指出“产业振兴是乡村振兴的重中之重,要落实产业帮扶政策,做好‘土特产’文章,依托农业农村特色资源,向开发农业多种功能、挖掘乡村多元价值要效益,向一二三产业融合发展要效益,强龙头、补链条、兴业态、树品牌,推动乡村产业全链条升级,增强市场竞争力和可持续发展能力”。本文深刻领会习近平总书记、中央一号文件、中共浙江省委十五届二次全会精神,通过深入总结嘉兴与丽水近二十年开展“山海协作”的成功经验,并针对嘉兴与丽水联动发展特色农业产业链目前遇到的种养技术、销售模式、专家团队、高技能人才等瓶颈,提出构建“嘉兴农产品专业市场与丽水种养基地联动发展特色农业产业链的‘W2F+B2M’(Wholesale to Farmer+Business to Member,即嘉兴农产品专业市场与丽水种养基地+嘉兴专业市场商户与商户的会员联动)模式”,进一步完善嘉兴与丽水特色农业产业链的联动发展机制,助推嘉兴与丽水共同富裕。展开更多
基金Supported by The National 11th 5-Year Key Programs for Department of Science and Technology of China, No. 2006BAI02A08
文摘AIM: To explore the value of serum M2-pyruvate kinase (M2-PK) in colorectal cancer (CRC) mass screening. METHODS: We conducted a molecular epidemiology study in Hangzhou, China, from year 2006 to year 2008. Serum samples were collected from 93 CRC, 41 advanced adenomas, 137 adenomas, 47 non-adenomatous polyps, and 158 normal participants in a community setting. Serum M2-PK and carcinoembryonic antigen (CEA) were measured using Enzyme-linked immunosorbent assay. SPSS 16.0 software was used to perform data analysis. Area under the receiver operating characteristic curve (AUC), sensitivity, and specificities were estimated for serum M2-PK in diagnosis of colorectal lesions and compared with CEA. RESULTS: Average serum M2-PK value among 158 normal people was 2.96 U/mL and not affected by gender (P = 0.47) or age (P = 0.59). Average serum M2-PK (U/mL) was 14.75 among stage III and 13.10 among stage?I?and II CRC patients, about 4 times higher than that among normal people. Average serum M2-PK was 8.58, 6.70, 5.13 and 2.51 U/mL among advanced adenoma, adenomas, non-adenomatous polyps, and inflammatory bowel disease patients, respectively. AUC for serum M2-PK was greater than that for CEA among all colorectal lesions. AUC for serum M2-PK was 0.89 (0.84, 0.94) (95% confidence interval), higher than that for CEA [0.70 (0.62-0.79)] in CRC stage?I?and II, 0.89 (0.84-0.94) vs 0.73 (0.63-0.83) in CRC stage III, 0.81 (0.74-0.86) vs 0.63 (0.53 - 0.73) in advanced adenomas, 0.69 (0.64-0.76) vs 0.54 (0.47-0.60) in adenomas, and 0.69 (0.62-0.78) vs 0.58 (0.48-0.68) in non-adenomatous polyps. The diagnostic sensitivity for all colorectal lesions increased with decrease in the cut-off value of serum M2-PK. The diagnostic sensitivity (%) of serum M2-PK was 100.00 for CRC, 95.12 advanced adenoma, 82.48 adenoma, and 82.98 non-adenomatous polyp. There were no CRC cases missed and 40.51% of unnecessary colonoscopies were avoided when the cut-off value was 2.00 U/mL. CONCLUSION: Serum M2-PK can be used as a primary screening test in CRC mass screening. It may be a promising non-invasive biomarker for CRC early detection.
文摘2022年中央一号文件《中共中央国务院关于做好2022年全面推进乡村振兴重点工作的意见》明确要求大力推进数字农业建设,推进智慧农业发展;2022年12月23日至24日中央农村工作会议上习近平总书记指出“产业振兴是乡村振兴的重中之重,要落实产业帮扶政策,做好‘土特产’文章,依托农业农村特色资源,向开发农业多种功能、挖掘乡村多元价值要效益,向一二三产业融合发展要效益,强龙头、补链条、兴业态、树品牌,推动乡村产业全链条升级,增强市场竞争力和可持续发展能力”。本文深刻领会习近平总书记、中央一号文件、中共浙江省委十五届二次全会精神,通过深入总结嘉兴与丽水近二十年开展“山海协作”的成功经验,并针对嘉兴与丽水联动发展特色农业产业链目前遇到的种养技术、销售模式、专家团队、高技能人才等瓶颈,提出构建“嘉兴农产品专业市场与丽水种养基地联动发展特色农业产业链的‘W2F+B2M’(Wholesale to Farmer+Business to Member,即嘉兴农产品专业市场与丽水种养基地+嘉兴专业市场商户与商户的会员联动)模式”,进一步完善嘉兴与丽水特色农业产业链的联动发展机制,助推嘉兴与丽水共同富裕。
