The preparation of silver nanoparticles(AgNPs)with microbe or plant tissues as bio-template offers green approach,while it suffers from low harvest and purification is needed.Herein,we propose a facile protocol for on...The preparation of silver nanoparticles(AgNPs)with microbe or plant tissues as bio-template offers green approach,while it suffers from low harvest and purification is needed.Herein,we propose a facile protocol for one-pot preparation of AgNPs using M13 phage as bio-template by simply mixing AgN03 solution with alkali M13 phage.In the obtained AgNPs-M13 phage composite,Cr(Ⅲ)selectively coordinates with the amino residues on phage surface and leads to the aggregation of AgNPs through the bridging of M13 phages.This makes it feasible for colorimetric sensing of Cr(Ⅲ)by measuring the absorbance ratio of AgNPs at 600 and 405 nm,which provides a LOD of 14 nmol/L.The composite also showed favorable bactericidal activity for both Gram-positive and Gram-negative bacteria,making it a promising candidate as antibacterial film in chromium-containing dental alloys and meanwhile serve as a sensing probe for monitoring the corrosion of the dental alloys.展开更多
The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane ha...The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon exposure to polar organic solvents. We show that this finding can be beneficial for the macromolecular self-assembly and in broader aspects, to enhance the spatial arrangement of desired inorganic nanoparticles in the rapidly developing field of virotronics.展开更多
M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins...M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.展开更多
As one of the most delicate bioprocesses in nature,biosilicification is closely related to biosilica with various morphologies,and has provided abundant inspiration to materials synthesis.In the present study,to explo...As one of the most delicate bioprocesses in nature,biosilicification is closely related to biosilica with various morphologies,and has provided abundant inspiration to materials synthesis.In the present study,to explore the biosilica formation process and fabricate silica with an exquisite microstructure for lithiumion battery(LIB)electrodes,a bacterial phage(M13)is used as a biotemplate to synthesize silica with diverse morphologies:cylinders,hexagonal prisms,assemblies of smaller cylinders and nanowires.A facile ethanol bath method is conducted to coat the nanowires with nitrogen-containing carbon and carbon-coated SiO_(2) nanowires with mesochannels(C@msSiO_(2) NWs)are first used as anode materials for LIBs.Attributed to the uniform carbon coating and parallel mesochannel structure,the electronic conductivity and capacity to accommodate volume variations were significantly improved.In the electrochemical perfo rmance test,the composites calcined at 750℃(C@msSiO_(2) NWs-750)show an impressive capacity of 653 mA h g^(-1) at a current density of 500 mA g^(-1) and stability(1000 cycles).In view of the electrochemical test outcomes,the prepa ration of a sophisticated structure with an outstanding potential is easily achieved via a biomimetic strategy.展开更多
基金Financial support from the National Natural Science Foundation of China (Nos.21874014,21727811,21675019,21605161)the Fundamental Research Funds for the Central Universities (No. N180505021)
文摘The preparation of silver nanoparticles(AgNPs)with microbe or plant tissues as bio-template offers green approach,while it suffers from low harvest and purification is needed.Herein,we propose a facile protocol for one-pot preparation of AgNPs using M13 phage as bio-template by simply mixing AgN03 solution with alkali M13 phage.In the obtained AgNPs-M13 phage composite,Cr(Ⅲ)selectively coordinates with the amino residues on phage surface and leads to the aggregation of AgNPs through the bridging of M13 phages.This makes it feasible for colorimetric sensing of Cr(Ⅲ)by measuring the absorbance ratio of AgNPs at 600 and 405 nm,which provides a LOD of 14 nmol/L.The composite also showed favorable bactericidal activity for both Gram-positive and Gram-negative bacteria,making it a promising candidate as antibacterial film in chromium-containing dental alloys and meanwhile serve as a sensing probe for monitoring the corrosion of the dental alloys.
文摘The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon exposure to polar organic solvents. We show that this finding can be beneficial for the macromolecular self-assembly and in broader aspects, to enhance the spatial arrangement of desired inorganic nanoparticles in the rapidly developing field of virotronics.
文摘M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.
基金supported financially by the National Natural Science Foundation of China(Nos.51521001,51832003,51902236 and 31771032)。
文摘As one of the most delicate bioprocesses in nature,biosilicification is closely related to biosilica with various morphologies,and has provided abundant inspiration to materials synthesis.In the present study,to explore the biosilica formation process and fabricate silica with an exquisite microstructure for lithiumion battery(LIB)electrodes,a bacterial phage(M13)is used as a biotemplate to synthesize silica with diverse morphologies:cylinders,hexagonal prisms,assemblies of smaller cylinders and nanowires.A facile ethanol bath method is conducted to coat the nanowires with nitrogen-containing carbon and carbon-coated SiO_(2) nanowires with mesochannels(C@msSiO_(2) NWs)are first used as anode materials for LIBs.Attributed to the uniform carbon coating and parallel mesochannel structure,the electronic conductivity and capacity to accommodate volume variations were significantly improved.In the electrochemical perfo rmance test,the composites calcined at 750℃(C@msSiO_(2) NWs-750)show an impressive capacity of 653 mA h g^(-1) at a current density of 500 mA g^(-1) and stability(1000 cycles).In view of the electrochemical test outcomes,the prepa ration of a sophisticated structure with an outstanding potential is easily achieved via a biomimetic strategy.
