Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DN...Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray Results Of the 102 rifampin-resistant strains 98 （96.1%） had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.展开更多
Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between M.intracellulare and M.tuberculosis.Methods Protein extracts from M.intracellulare w...Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between M.intracellulare and M.tuberculosis.Methods Protein extracts from M.intracellulare were used to immunize BALB/c mice.The antigens were evaluated using cellular and humoral immunoassays.The common genes between M.intracellular and M.tuberculosis were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome microarrays.Results Immunization with M.intracellulare proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse serum.Bone marrow-derived macrophages isolated from mice immunized with M.intracellulare antigens displayed significantly lower bacillary loads than those isolated from mice immunized with adjuvants.Whole-genome sequence analysis revealed 396 common genes between M.intracellulare and M.tuberculosis.Microchip hybridization with M.tuberculosis proteins revealed the presence of 478 proteins in the serum of mice immunized with M.intracellulare protein extracts.Sixty common antigens were found using both microchip and genomic comparative analyses.Conclusion This is the advanced study to investigate the immunogenicity of M.intracellulare proteins and the cross-reactive proteins between M.intracellulare and M.tuberculosis.The results revealed the presence of a number of cross-reactive proteins between M.intracellulare and M.tuberculosis.Therefore,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both M.intracellulare and M.tuberculosis in future.展开更多
背景:类风湿关节炎是以持续性滑膜炎和进行性骨破坏为主要病理特征的慢性自身免疫性疾病。巨噬细胞作为关键的效应细胞,主要通过极化成不同功能表型,在类风湿关节炎发病机制中发挥核心作用。目的:综述巨噬细胞极化在类风湿关节炎骨破坏...背景:类风湿关节炎是以持续性滑膜炎和进行性骨破坏为主要病理特征的慢性自身免疫性疾病。巨噬细胞作为关键的效应细胞,主要通过极化成不同功能表型,在类风湿关节炎发病机制中发挥核心作用。目的:综述巨噬细胞极化在类风湿关节炎骨破坏中的作用及调控机制和最新治疗进展。方法:利用计算机检索Web of Science核心数据库、万方数据库和中国知网2005-2024年期间发表的相关文献。中文检索词为“巨噬细胞,类风湿关节炎,极化,骨与关节,软骨,自身免疫,炎症,M1巨噬细胞,M2巨噬细胞”,英文检索词为“macrophages,rheumatoid arthritis,polarization,bone and Joints,cartilage,autoimmunity,inflammation,M1 macrophage,M2 macrophage”,最终对53篇文献展开综述。结果与结论:类风湿关节炎是一种慢性炎症性疾病,以持续炎症性骨破坏为特征,如果治疗不当,可导致关节畸形,甚至功能丧失。巨噬细胞M1/M2极化比例失衡是类风湿关节炎疾病进展的关键点,这强调了研究巨噬细胞极化在类风湿关节炎炎症性骨破坏中的作用及调控机制的必要性,巨噬细胞极化调控机制能够作为新型治疗剂的潜在靶点。展开更多
基金supported by the grant from the National Natural Science Foundation of China (No. 30400018)
文摘Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray Results Of the 102 rifampin-resistant strains 98 （96.1%） had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
基金supported by National Science and Technology Major Project of China[2018ZX10731301-002]。
文摘Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between M.intracellulare and M.tuberculosis.Methods Protein extracts from M.intracellulare were used to immunize BALB/c mice.The antigens were evaluated using cellular and humoral immunoassays.The common genes between M.intracellular and M.tuberculosis were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome microarrays.Results Immunization with M.intracellulare proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse serum.Bone marrow-derived macrophages isolated from mice immunized with M.intracellulare antigens displayed significantly lower bacillary loads than those isolated from mice immunized with adjuvants.Whole-genome sequence analysis revealed 396 common genes between M.intracellulare and M.tuberculosis.Microchip hybridization with M.tuberculosis proteins revealed the presence of 478 proteins in the serum of mice immunized with M.intracellulare protein extracts.Sixty common antigens were found using both microchip and genomic comparative analyses.Conclusion This is the advanced study to investigate the immunogenicity of M.intracellulare proteins and the cross-reactive proteins between M.intracellulare and M.tuberculosis.The results revealed the presence of a number of cross-reactive proteins between M.intracellulare and M.tuberculosis.Therefore,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both M.intracellulare and M.tuberculosis in future.
文摘背景:类风湿关节炎是以持续性滑膜炎和进行性骨破坏为主要病理特征的慢性自身免疫性疾病。巨噬细胞作为关键的效应细胞,主要通过极化成不同功能表型,在类风湿关节炎发病机制中发挥核心作用。目的:综述巨噬细胞极化在类风湿关节炎骨破坏中的作用及调控机制和最新治疗进展。方法:利用计算机检索Web of Science核心数据库、万方数据库和中国知网2005-2024年期间发表的相关文献。中文检索词为“巨噬细胞,类风湿关节炎,极化,骨与关节,软骨,自身免疫,炎症,M1巨噬细胞,M2巨噬细胞”,英文检索词为“macrophages,rheumatoid arthritis,polarization,bone and Joints,cartilage,autoimmunity,inflammation,M1 macrophage,M2 macrophage”,最终对53篇文献展开综述。结果与结论:类风湿关节炎是一种慢性炎症性疾病,以持续炎症性骨破坏为特征,如果治疗不当,可导致关节畸形,甚至功能丧失。巨噬细胞M1/M2极化比例失衡是类风湿关节炎疾病进展的关键点,这强调了研究巨噬细胞极化在类风湿关节炎炎症性骨破坏中的作用及调控机制的必要性,巨噬细胞极化调控机制能够作为新型治疗剂的潜在靶点。
