Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have i...Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell death.However,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking.We performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite levels.We found that cell death and Magnaporthe oryzae(M.oryzae)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory layers.We characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance.SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M.oryzae.Our studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.展开更多
Blast diseases of rice and wheat are known to be caused by the specific pathotypes of Magnaporthe oryzae(syn.Pyricularia oryzae),M.oryzae Oryzae(MoO)and M.oryzae Triticum(MoT),respectively.Rice blast disease has been ...Blast diseases of rice and wheat are known to be caused by the specific pathotypes of Magnaporthe oryzae(syn.Pyricularia oryzae),M.oryzae Oryzae(MoO)and M.oryzae Triticum(MoT),respectively.Rice blast disease has been seen in Bangladesh from a very ancient time.However,Bangladesh’s first epidemic outbreak of wheat blast was recorded in 2016.This study aimed to investigate the cross-infection reactions of MoO and MoT in rice and wheat in a growth room condition.Artificial inoculation was done at vegetative and reproductive phases of both wheat and rice plants in a completely randomized design using virulent isolates of MoO and MoT.Artificial inoculation with MoO resulted in foliar symptoms with typical eye-shaped lesions as well as partially bleached or completely white head symptoms in both wheat and rice plants.On the other hand,MoT produced blast symptoms only on the leaves and spikes of wheat.Molecular analyses using PCR amplification(with Pot2,MoT3 and MoT6099 primers)and a recently developed rapid detection PCRD strip confirmed the presence of MoT and MoO pathotypes in the symptomatic plant samples.Our results demonstrated that MoO pathotype can infect the leaves and spikes of wheat but MoT is unable to infect rice plants under the same controlled environment in Bangladesh.This study has revealed the vulnerability of wheat to MoO pathotype and an urgent need to understand the molecular mechanism underlying host-specificity of the blast fungus M.oryzae.Our results also provided evidence for a potential wheat blast epidemic by MoO in many rice-wheat inter-cropping regions as climate change intensifies.A comprehensive study is needed to have a better understanding on the variability in virulence of MoO and MoT isolates in infecting wheat and rice under controlled environment by the inclusion of a large number of isolates and crop varieties/genotypes.展开更多
基金supported by the Collaborative Innovation Engineering“5511”(XTCXGC2021002)the National Natural Science Foundation of China(U1805232)+1 种基金the Youth Program of National Natural Science Foundation of China(31301654)the Youth Program of Fujian Academy of Agricultural Sciences(YC2019004)。
文摘Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell death.However,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking.We performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite levels.We found that cell death and Magnaporthe oryzae(M.oryzae)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory layers.We characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance.SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M.oryzae.Our studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.
基金supported by grants from the SEEDS program of the Ohio Agricultural Research and Development Center(OARDC)and the Coordinated Research Project(D23032)of the International Atomic Energy Agency(IAEA)to GLW and TIsupport from the Krishi Gobeshona Foundation(KGF)of Bangladesh under projects Nos.KGF TF50-C/17 and TF 92-FNS/21 to Tofazzal Islam of the IBGE of BSMRAU,Bangladesh is also acknowledged.
文摘Blast diseases of rice and wheat are known to be caused by the specific pathotypes of Magnaporthe oryzae(syn.Pyricularia oryzae),M.oryzae Oryzae(MoO)and M.oryzae Triticum(MoT),respectively.Rice blast disease has been seen in Bangladesh from a very ancient time.However,Bangladesh’s first epidemic outbreak of wheat blast was recorded in 2016.This study aimed to investigate the cross-infection reactions of MoO and MoT in rice and wheat in a growth room condition.Artificial inoculation was done at vegetative and reproductive phases of both wheat and rice plants in a completely randomized design using virulent isolates of MoO and MoT.Artificial inoculation with MoO resulted in foliar symptoms with typical eye-shaped lesions as well as partially bleached or completely white head symptoms in both wheat and rice plants.On the other hand,MoT produced blast symptoms only on the leaves and spikes of wheat.Molecular analyses using PCR amplification(with Pot2,MoT3 and MoT6099 primers)and a recently developed rapid detection PCRD strip confirmed the presence of MoT and MoO pathotypes in the symptomatic plant samples.Our results demonstrated that MoO pathotype can infect the leaves and spikes of wheat but MoT is unable to infect rice plants under the same controlled environment in Bangladesh.This study has revealed the vulnerability of wheat to MoO pathotype and an urgent need to understand the molecular mechanism underlying host-specificity of the blast fungus M.oryzae.Our results also provided evidence for a potential wheat blast epidemic by MoO in many rice-wheat inter-cropping regions as climate change intensifies.A comprehensive study is needed to have a better understanding on the variability in virulence of MoO and MoT isolates in infecting wheat and rice under controlled environment by the inclusion of a large number of isolates and crop varieties/genotypes.
