Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making ...Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making it a promising target for CH_(4) mitigation.This study aimed to identify and validate plant-derived inhibitors by using molecular docking to screen compounds with strong binding affinity to the F430 active site of MCR and assessing their efficacy in reducing CH_(4) emissions.Results Molecular docking analysis identified salvianolic acid C(SAC)as a potent inhibitor of MCR,showing a strong binding affinity to the F430 active site(binding energy:-8.2 kcal/mol).Enzymatic inhibition assays confirmed its inhibitory effect,with a half-maximal inhibitory concentration(IC50)of 692.3μmol/L.In vitro rumen fermentation experiments demonstrated that SAC supplementation(1.5 mg/g DM)significantly reduced CH_(4)production(P<0.01)without negatively affecting major fermentation parameters.Microbial community analysis using 16S rRNA sequencing and metagenomics revealed that SAC selectively altered the rumen microbiota,increasing the relative abundance of Bacteroidota while significantly reducing Methanobrevibacter(P=0.04).Moreover,metagenomic analysis showed the downregulation of key methanogenesis-related genes(mcrA and rnfC),suggesting a dual mechanism involving direct enzymatic inhibition and microbial community modulation.Conclusions These findings indicate that SAC effectively reduces CH_(4)production by inhibiting MCR activity and reshaping the rumen microbial community.As a plant-derived compound with strong inhibitory effects on methanogenesis,SAC presents a promising and sustainable alternative to synthetic CH_(4) inhibitors,offering potential applications for mitigating CH_(4)emissions in livestock production.展开更多
The mitochondrial 3243A>G mutation(m.3243A>G)is associated with diverse clinical phenotypes.To elucidate the underlying mechanisms and explore intervention strategies in m.3243A>G patients,urine-derived stem ...The mitochondrial 3243A>G mutation(m.3243A>G)is associated with diverse clinical phenotypes.To elucidate the underlying mechanisms and explore intervention strategies in m.3243A>G patients,urine-derived stem cells(USCs)and a mitochondrial leucyl-tRNA synthetase gene(lars-2)deficient Caenorhabditis elegans(C.elegans)model are used to assess mitochondrial homeostasis and neuromuscular dysfunction.Patient-derived USCs with high levels of m.3243A>G heteroplasmy exhibit impaired mitochondrial function,disrupted mitochondrial dynamics,and inhibited mitophagy,which are reversed by MitoQ through suppression of OMA1 zinc metallopeptidase(OMA1)-induced mitochondrial phosphatase and tensin(PTEN)induced kinase 1(PINK1)degradation.Furthermore,lars-2 knockdown in C.elegans induces mitochondrial stress and mimics the loss of neural and muscle functions observed in patients with the m.3243A>G mutation.MitoQ treatment partially improves neurobehavioral function by promoting the PINK1 pathway.These findings suggest that MitoQ has therapeutic potential in the context of the m.3243A>G mutation.展开更多
“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此...“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此背景下,由贝壳集团旗下贝好家推出的C2M(Customer to Manufacturer,从消费者到制造者)全链解决方案,经过一段时间的探索和落地实践,已取得不错的实践成效和市场反馈。展开更多
To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuou...To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuous rotation electron diffraction(cryo-cRED)with a low-dose strategy to determine the crystal structure of CL30,a novel silicogermanate framework.It is confirmed that CL30 crystallizes in the C2/m space group and has layered topology composed of discontinuous zigzag chains connected by double four-membered ring(d4r)units,with fluoride anions(F^(-))occluded in the d4r units.In CL30,charge balance involves organic structure-directing agent(OSDA)cations,occluded F^(-),and terminal oxygen sites whose protonation state cannot be established from the present three dimensional(3D)ED data.F^(-)encapsulated in the d4r units contributes to charge compensation as the counter-anion to OSDA cations,rather than only balancing the framework charge.Although the refinement indices(R_(1)=0.29,wR_(2)=0.71)exceeded typical small-molecule crystallography standards,the structural model remained highly reliable,as supported by geometric restraints and validation.In electron diffraction,elevated R_(1) values are commonly attributed to the intrinsic factors of the technique,such as dynamic scattering,detector noise from scintillator-based detectors,and TEM stage instability(large spheres of confusion).This study introduces a new structural prototype to the silicogermanate family and establishes a feasible workflow for determining the structures of radiation-sensitive microcrystalline porous materials.展开更多
In this paper, using the stochastic decomposition and renewal theory we provide the direct method for analysis the departure process of single sever M/G/1 queueing system, and further discuss the departure process of ...In this paper, using the stochastic decomposition and renewal theory we provide the direct method for analysis the departure process of single sever M/G/1 queueing system, and further discuss the departure process of GI/G/1 queueing system. The method provided in this paper is new and concise, which make us see dearly the structure of the departure process of a single server queueing system.展开更多
By studying the spectral properties of the underlying operator corresponding to the M/G/1 queueing model with optional second service we obtain that the time-dependent solution of the model strongly converges to its s...By studying the spectral properties of the underlying operator corresponding to the M/G/1 queueing model with optional second service we obtain that the time-dependent solution of the model strongly converges to its steady-state solution. We also show that the time-dependent queueing size at the departure point converges to the corresponding steady-state queueing size at the departure point.展开更多
In this paper, we consider point spectra of the operator corresponding to the M/M/1 queueing model with working vacation and vacation interruption. We prove that the underlying operator has uncountable eigenvalues on ...In this paper, we consider point spectra of the operator corresponding to the M/M/1 queueing model with working vacation and vacation interruption. We prove that the underlying operator has uncountable eigenvalues on the left real line and these results describe the point spectra of the operator. Then, we show that the essential growth bound of the C_0-semigroup generated by the operator is 0 and therefore it is not quasi compact, the essential spectral bound of the C_0-semigroup is equal to 1. Moreover, our results imply it is impossible that the time-dependent solution of the model exponentially converges to its steady-state solution.展开更多
It is well known, in queueing theory, that the system performance is greatly influenced by scheduling policy. No universal optimum scheduling strategy exists in systems where individual customer service demands are no...It is well known, in queueing theory, that the system performance is greatly influenced by scheduling policy. No universal optimum scheduling strategy exists in systems where individual customer service demands are not known a priori. However, if the distribution of job times is known, then the residual time (expected time remaining for a job), based on the service it has already received, can be calculated. Our particular research contribution is in exploring the use of this function to enhance system performance by increasing the probability that a job will meet its deadline. In a detailed discrete event simulation, we have tested many different distributions with a wide range of C2 and shapes, as well as for single and dual processor system. Results of four distributions are reported here. We compare with RR and FCFS, and find that in all distributions studied our algorithm performs best. In the study of the use of two slow servers versus one fast server, we have discovered that they provide comparable performance, and in a few cases the double server system does better.展开更多
The Distributed Queuing (DQ) algorithm is predicted as one of the solutions to the issues currently found in IoT networks over the use of Aloha based algorithms. Since recently, the algorithm has been of interest to m...The Distributed Queuing (DQ) algorithm is predicted as one of the solutions to the issues currently found in IoT networks over the use of Aloha based algorithms. Since recently, the algorithm has been of interest to many IoT researchers as a replacement of those Aloha variants for channel access. However, previous works analyzed and evaluated the DQ algorithm without any consideration of the stability of its queues, assuming it is stable for any given number of nodes in the network. In this paper, we define the DQ stability condition in a single-channel M2M environment considering a traffic model of periodic and urgent frames from each node in the network. Besides, a steady-state evaluation of the algorithm’s performance metrics is also presented. In general, the DQ algorithm, when it is stable, was observed not to efficiently use the contention slots for the collision resolution. In a single-channel environment, the DQ algorithm is found to outperform the Aloha based algorithms only in an idle-to-saturation scenario.展开更多
基金funded by the Integrated Demonstration of Scalable and Efficient Healthy Breeding for Cattle and Sheep(Grant No.2022YFD1301100)Instant Intelligent Diagnosis and Risk Warning Methods for Nutritional and Metabolic-Type Periparturient Cow Paralysis(Grant No.2024-YWF-ZYSQ-10)。
文摘Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making it a promising target for CH_(4) mitigation.This study aimed to identify and validate plant-derived inhibitors by using molecular docking to screen compounds with strong binding affinity to the F430 active site of MCR and assessing their efficacy in reducing CH_(4) emissions.Results Molecular docking analysis identified salvianolic acid C(SAC)as a potent inhibitor of MCR,showing a strong binding affinity to the F430 active site(binding energy:-8.2 kcal/mol).Enzymatic inhibition assays confirmed its inhibitory effect,with a half-maximal inhibitory concentration(IC50)of 692.3μmol/L.In vitro rumen fermentation experiments demonstrated that SAC supplementation(1.5 mg/g DM)significantly reduced CH_(4)production(P<0.01)without negatively affecting major fermentation parameters.Microbial community analysis using 16S rRNA sequencing and metagenomics revealed that SAC selectively altered the rumen microbiota,increasing the relative abundance of Bacteroidota while significantly reducing Methanobrevibacter(P=0.04).Moreover,metagenomic analysis showed the downregulation of key methanogenesis-related genes(mcrA and rnfC),suggesting a dual mechanism involving direct enzymatic inhibition and microbial community modulation.Conclusions These findings indicate that SAC effectively reduces CH_(4)production by inhibiting MCR activity and reshaping the rumen microbial community.As a plant-derived compound with strong inhibitory effects on methanogenesis,SAC presents a promising and sustainable alternative to synthetic CH_(4) inhibitors,offering potential applications for mitigating CH_(4)emissions in livestock production.
基金funded by the NIH Office of Research Infrastructure Programs(P40 OD010440)supported by the National Natural Science Foundation of China(82471893 and 82070913)+2 种基金Key discipline project of Hongkou District Health Commission(HKLCFC202403)Tongji Hospital Start-up Funding for Scientific Research(RCQD2301)Research fund from Shanghai Fourth People's Hospital(sykyqd01801,SYXKZT-2021-1001).
文摘The mitochondrial 3243A>G mutation(m.3243A>G)is associated with diverse clinical phenotypes.To elucidate the underlying mechanisms and explore intervention strategies in m.3243A>G patients,urine-derived stem cells(USCs)and a mitochondrial leucyl-tRNA synthetase gene(lars-2)deficient Caenorhabditis elegans(C.elegans)model are used to assess mitochondrial homeostasis and neuromuscular dysfunction.Patient-derived USCs with high levels of m.3243A>G heteroplasmy exhibit impaired mitochondrial function,disrupted mitochondrial dynamics,and inhibited mitophagy,which are reversed by MitoQ through suppression of OMA1 zinc metallopeptidase(OMA1)-induced mitochondrial phosphatase and tensin(PTEN)induced kinase 1(PINK1)degradation.Furthermore,lars-2 knockdown in C.elegans induces mitochondrial stress and mimics the loss of neural and muscle functions observed in patients with the m.3243A>G mutation.MitoQ treatment partially improves neurobehavioral function by promoting the PINK1 pathway.These findings suggest that MitoQ has therapeutic potential in the context of the m.3243A>G mutation.
文摘“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此背景下,由贝壳集团旗下贝好家推出的C2M(Customer to Manufacturer,从消费者到制造者)全链解决方案,经过一段时间的探索和落地实践,已取得不错的实践成效和市场反馈。
基金supported by the National Natural Science Foundation of China (Grant No.12374021)Beijing Natural Science Foundation (Grant No.1252031)。
文摘To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuous rotation electron diffraction(cryo-cRED)with a low-dose strategy to determine the crystal structure of CL30,a novel silicogermanate framework.It is confirmed that CL30 crystallizes in the C2/m space group and has layered topology composed of discontinuous zigzag chains connected by double four-membered ring(d4r)units,with fluoride anions(F^(-))occluded in the d4r units.In CL30,charge balance involves organic structure-directing agent(OSDA)cations,occluded F^(-),and terminal oxygen sites whose protonation state cannot be established from the present three dimensional(3D)ED data.F^(-)encapsulated in the d4r units contributes to charge compensation as the counter-anion to OSDA cations,rather than only balancing the framework charge.Although the refinement indices(R_(1)=0.29,wR_(2)=0.71)exceeded typical small-molecule crystallography standards,the structural model remained highly reliable,as supported by geometric restraints and validation.In electron diffraction,elevated R_(1) values are commonly attributed to the intrinsic factors of the technique,such as dynamic scattering,detector noise from scintillator-based detectors,and TEM stage instability(large spheres of confusion).This study introduces a new structural prototype to the silicogermanate family and establishes a feasible workflow for determining the structures of radiation-sensitive microcrystalline porous materials.
文摘In this paper, using the stochastic decomposition and renewal theory we provide the direct method for analysis the departure process of single sever M/G/1 queueing system, and further discuss the departure process of GI/G/1 queueing system. The method provided in this paper is new and concise, which make us see dearly the structure of the departure process of a single server queueing system.
基金supported by the National Natural Science Foundation of China(11371303)Natural Science Foundation of Xinjiang(2012211A023)Science Foundation of Xinjiang University(XY110101)
文摘By studying the spectral properties of the underlying operator corresponding to the M/G/1 queueing model with optional second service we obtain that the time-dependent solution of the model strongly converges to its steady-state solution. We also show that the time-dependent queueing size at the departure point converges to the corresponding steady-state queueing size at the departure point.
基金Supported by the National Natural Science Foundation of China(Grant No.11801485)
文摘In this paper, we consider point spectra of the operator corresponding to the M/M/1 queueing model with working vacation and vacation interruption. We prove that the underlying operator has uncountable eigenvalues on the left real line and these results describe the point spectra of the operator. Then, we show that the essential growth bound of the C_0-semigroup generated by the operator is 0 and therefore it is not quasi compact, the essential spectral bound of the C_0-semigroup is equal to 1. Moreover, our results imply it is impossible that the time-dependent solution of the model exponentially converges to its steady-state solution.
文摘It is well known, in queueing theory, that the system performance is greatly influenced by scheduling policy. No universal optimum scheduling strategy exists in systems where individual customer service demands are not known a priori. However, if the distribution of job times is known, then the residual time (expected time remaining for a job), based on the service it has already received, can be calculated. Our particular research contribution is in exploring the use of this function to enhance system performance by increasing the probability that a job will meet its deadline. In a detailed discrete event simulation, we have tested many different distributions with a wide range of C2 and shapes, as well as for single and dual processor system. Results of four distributions are reported here. We compare with RR and FCFS, and find that in all distributions studied our algorithm performs best. In the study of the use of two slow servers versus one fast server, we have discovered that they provide comparable performance, and in a few cases the double server system does better.
文摘The Distributed Queuing (DQ) algorithm is predicted as one of the solutions to the issues currently found in IoT networks over the use of Aloha based algorithms. Since recently, the algorithm has been of interest to many IoT researchers as a replacement of those Aloha variants for channel access. However, previous works analyzed and evaluated the DQ algorithm without any consideration of the stability of its queues, assuming it is stable for any given number of nodes in the network. In this paper, we define the DQ stability condition in a single-channel M2M environment considering a traffic model of periodic and urgent frames from each node in the network. Besides, a steady-state evaluation of the algorithm’s performance metrics is also presented. In general, the DQ algorithm, when it is stable, was observed not to efficiently use the contention slots for the collision resolution. In a single-channel environment, the DQ algorithm is found to outperform the Aloha based algorithms only in an idle-to-saturation scenario.
