Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making ...Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making it a promising target for CH_(4) mitigation.This study aimed to identify and validate plant-derived inhibitors by using molecular docking to screen compounds with strong binding affinity to the F430 active site of MCR and assessing their efficacy in reducing CH_(4) emissions.Results Molecular docking analysis identified salvianolic acid C(SAC)as a potent inhibitor of MCR,showing a strong binding affinity to the F430 active site(binding energy:-8.2 kcal/mol).Enzymatic inhibition assays confirmed its inhibitory effect,with a half-maximal inhibitory concentration(IC50)of 692.3μmol/L.In vitro rumen fermentation experiments demonstrated that SAC supplementation(1.5 mg/g DM)significantly reduced CH_(4)production(P<0.01)without negatively affecting major fermentation parameters.Microbial community analysis using 16S rRNA sequencing and metagenomics revealed that SAC selectively altered the rumen microbiota,increasing the relative abundance of Bacteroidota while significantly reducing Methanobrevibacter(P=0.04).Moreover,metagenomic analysis showed the downregulation of key methanogenesis-related genes(mcrA and rnfC),suggesting a dual mechanism involving direct enzymatic inhibition and microbial community modulation.Conclusions These findings indicate that SAC effectively reduces CH_(4)production by inhibiting MCR activity and reshaping the rumen microbial community.As a plant-derived compound with strong inhibitory effects on methanogenesis,SAC presents a promising and sustainable alternative to synthetic CH_(4) inhibitors,offering potential applications for mitigating CH_(4)emissions in livestock production.展开更多
“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此...“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此背景下,由贝壳集团旗下贝好家推出的C2M(Customer to Manufacturer,从消费者到制造者)全链解决方案,经过一段时间的探索和落地实践,已取得不错的实践成效和市场反馈。展开更多
Improving mechanical quality factor Q_(m) is of great significance for high-power applications.Here,a new strategy of the[111]_(c) texture engineering was proposed to enhance the performances of high-power piezoelectr...Improving mechanical quality factor Q_(m) is of great significance for high-power applications.Here,a new strategy of the[111]_(c) texture engineering was proposed to enhance the performances of high-power piezoelectric ceramics.The 5 vol%BaTiO_(3)(BT)templates with the[111]c preferred orientation were in-troduced into matrix powders of 0.03 Pb(Mn_(1/3) Nb_(2/3))O_(3)-0.33Pb(Ni_(1/3) Nb_(2/3))O_(3)-0.28 PbZrO_(3)-0.36PbTiO_(3)(28PZ(R))to form the[111]c textured ceramics(28PZ(T)),possessing a texture degree of 74%.The mul-tiple of uniform density in EBSD increased from 0.63 in randomly oriented 28 PZ(R)to 6.63 in 28PZ(T).The good lattice matching between BT templates and textured grains was observed using high-resolution transmission electron microscopy,confirming the microscopic origin of the[111]c texture.The maximum phase angleθmax of 88.2°was quite near 90°in 28PZ(T),ensuring the optimal Qm value of 1275 and the great figure of merit of 255,000 pC/N.The increased Q_(m) in[111]c texture ceramics was confirmed due to the reduced intrinsic polarization directions rather than the pinning effect of the internal bias field.Larger grain sizes with larger domains restrained the movement of domain walls in 28 PZ(T),which was also favorable to higher Q_(m).This work may provide a new promising route for further high-power applications.展开更多
基金funded by the Integrated Demonstration of Scalable and Efficient Healthy Breeding for Cattle and Sheep(Grant No.2022YFD1301100)Instant Intelligent Diagnosis and Risk Warning Methods for Nutritional and Metabolic-Type Periparturient Cow Paralysis(Grant No.2024-YWF-ZYSQ-10)。
文摘Background Methane(CH_(4))emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production.Methyl-coenzyme M reductase(MCR)is the key enzyme in methanogenesis,making it a promising target for CH_(4) mitigation.This study aimed to identify and validate plant-derived inhibitors by using molecular docking to screen compounds with strong binding affinity to the F430 active site of MCR and assessing their efficacy in reducing CH_(4) emissions.Results Molecular docking analysis identified salvianolic acid C(SAC)as a potent inhibitor of MCR,showing a strong binding affinity to the F430 active site(binding energy:-8.2 kcal/mol).Enzymatic inhibition assays confirmed its inhibitory effect,with a half-maximal inhibitory concentration(IC50)of 692.3μmol/L.In vitro rumen fermentation experiments demonstrated that SAC supplementation(1.5 mg/g DM)significantly reduced CH_(4)production(P<0.01)without negatively affecting major fermentation parameters.Microbial community analysis using 16S rRNA sequencing and metagenomics revealed that SAC selectively altered the rumen microbiota,increasing the relative abundance of Bacteroidota while significantly reducing Methanobrevibacter(P=0.04).Moreover,metagenomic analysis showed the downregulation of key methanogenesis-related genes(mcrA and rnfC),suggesting a dual mechanism involving direct enzymatic inhibition and microbial community modulation.Conclusions These findings indicate that SAC effectively reduces CH_(4)production by inhibiting MCR activity and reshaping the rumen microbial community.As a plant-derived compound with strong inhibitory effects on methanogenesis,SAC presents a promising and sustainable alternative to synthetic CH_(4) inhibitors,offering potential applications for mitigating CH_(4)emissions in livestock production.
文摘“好房子”承载着千家万户对美好生活的向往,2025年被首次写入政府工作报告,住房城乡建设部及各地行业主管部门相继对“好房子”建设提出了一系列要求,并持续推动政策落地,建设“好房子”由此成为房地产领域产业转型发展的新赛道。在此背景下,由贝壳集团旗下贝好家推出的C2M(Customer to Manufacturer,从消费者到制造者)全链解决方案,经过一段时间的探索和落地实践,已取得不错的实践成效和市场反馈。
基金supported by the Natural Science Foundation of Heilongjiang Province(No.LH2021E052)the National Natural Science Foundation of China(Grant Nos.52202131 and 52002093)+1 种基金the Fundamental Research Funds for the Central Universities(Nos.HIT.NSRIF202313 and HIT.NSRIF202214)the National Key Research and Development Program of China(No.2022YFB3403902).
文摘Improving mechanical quality factor Q_(m) is of great significance for high-power applications.Here,a new strategy of the[111]_(c) texture engineering was proposed to enhance the performances of high-power piezoelectric ceramics.The 5 vol%BaTiO_(3)(BT)templates with the[111]c preferred orientation were in-troduced into matrix powders of 0.03 Pb(Mn_(1/3) Nb_(2/3))O_(3)-0.33Pb(Ni_(1/3) Nb_(2/3))O_(3)-0.28 PbZrO_(3)-0.36PbTiO_(3)(28PZ(R))to form the[111]c textured ceramics(28PZ(T)),possessing a texture degree of 74%.The mul-tiple of uniform density in EBSD increased from 0.63 in randomly oriented 28 PZ(R)to 6.63 in 28PZ(T).The good lattice matching between BT templates and textured grains was observed using high-resolution transmission electron microscopy,confirming the microscopic origin of the[111]c texture.The maximum phase angleθmax of 88.2°was quite near 90°in 28PZ(T),ensuring the optimal Qm value of 1275 and the great figure of merit of 255,000 pC/N.The increased Q_(m) in[111]c texture ceramics was confirmed due to the reduced intrinsic polarization directions rather than the pinning effect of the internal bias field.Larger grain sizes with larger domains restrained the movement of domain walls in 28 PZ(T),which was also favorable to higher Q_(m).This work may provide a new promising route for further high-power applications.
