Objective:To explore the interference of monoclonal immunoglobulin(M protein)on the detection of serum LDL-C in patients with multiple myeloma,improve the understanding of this matter,determine and establish the corre...Objective:To explore the interference of monoclonal immunoglobulin(M protein)on the detection of serum LDL-C in patients with multiple myeloma,improve the understanding of this matter,determine and establish the correct method,and provide more accurate clinical results through this case.Methods:A case was selected for analysis by the direct method.Results:The interference of IgG kappa-type M protein on LDL-C detection could not be completely eliminated by the enzymatic method.Conclusion:IgG-type type M protein affects the detection of LDL-C by the enzymatic method;thus,light reagents can be used with the direct method for detection.展开更多
In this paper, we report a multiple sequence alignment result on the basis of 10 amino acid sequences of the M protein, which come from different coronaviruses (4 SARS associated and 6 others known). The alignment mo...In this paper, we report a multiple sequence alignment result on the basis of 10 amino acid sequences of the M protein, which come from different coronaviruses (4 SARS associated and 6 others known). The alignment model was based on the profile HMM (Hidden Markov Model), and the model training was implemented through the SAHMM (Self Adapting Hidden Markov Model) software developed by the authors.展开更多
Group A streptococcus （GAS）, an important human pathogen, can cause various kinds of infections including superficial infections and potentially lethal infections, and the search for an effective vaccine to prevent ...Group A streptococcus （GAS）, an important human pathogen, can cause various kinds of infections including superficial infections and potentially lethal infections, and the search for an effective vaccine to prevent GAS infections has been ongoing for many years. This paper compares the immunogenicity and immunoprotection of FbaA （an Fn-binding protein expressed on the surface of GAS） with that of M protein, the best immunogen of GAS. Assay for immune response showed that FbaA, similar to M protein, could induce protein-specific high IgG titer in BALB/c mice. Furthermore, following GAS challenge, the mice immunized with FbaA showed the same protective rate as those with M protein. These results indicate that FbaA is similar in ability to M protein in inducing protective immunity against GAS challenge in mice. Cellular ＆ Molecular Immunology.展开更多
Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Ar...Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Arsenazo M itself. The apparent molar absorptivity of the BSA-Arsenazo M complex is 3.21105 Lmol-1cm-1. The linear ranges for protein determination are wide (at least 0-100 mg/mL).展开更多
We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predi...We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.展开更多
BACKGROUND Studies have shown that insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)plays critical roles in the genesis and development of human cancers.AIM To investigate the clinical significance and role...BACKGROUND Studies have shown that insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)plays critical roles in the genesis and development of human cancers.AIM To investigate the clinical significance and role of IGF2BP1 in pancreatic cancer.METHODS Expression levels of IGF2BP1 and microRNA-494(miR-494)were mined based on Gene Expression Omnibus datasets and validated in both clinical samples and cell lines by quantitative real-time polymerase chain reaction and Western blot.The relationship between IGF2BP1 expression and clinicopathological factors of pancreatic cancer patients was analyzed.The effect and mechanism of IGF2BP1 on pancreatic cancer cell proliferation were investigated in vitro and in vivo.Analyses were performed to explore underlying mechanisms of IGF2BP1 upregulation in pancreatic cancer and assays were carried out to verify the posttranscriptional regulation of IGF2BP1 by miR-494.RESULTS We found that IGF2BP1 was upregulated and associated with a poor prognosis in pancreatic cancer patients.We showed that downregulation of IGF2BP1 inhibited pancreatic cancer cell growth in vitro and in vivo via the AKT signaling pathway.Mechanistically,we showed that the frequent upregulation of IGF2BP1 was attributed to the downregulation of miR-494 expression in pancreatic cancer.Furthermore,we discovered that reexpression of miR-494 could partially abrogate the oncogenic role of IGF2BP1.CONCLUSION Our results revealed that upregulated IGF2BP1 promotes the proliferation of pancreatic cancer cells via the AKT signaling pathway and confirmed that the activation of IGF2BP1 is partly due to the silencing of miR-494.展开更多
Background： To investigate the effects of dietary crude protein（CP） restriction on muscle fiber characteristics and key regulators related to protein deposition in skeletal muscle, a total of 18 growing-finishing p...Background： To investigate the effects of dietary crude protein（CP） restriction on muscle fiber characteristics and key regulators related to protein deposition in skeletal muscle, a total of 18 growing-finishing pigs（62.30 ± 0.88 kg）were allotted to 3 groups and fed with the recommended adequate protein（AP, 16 % CP） diet, moderately restricted protein（MP, 13 % CP） diet and low protein（LP, 10 % CP） diet, respectively. The skeletal muscle of different locations in pigs, including longissimus dorsi muscle（LDM）, psoas major muscle（PMM） and biceps femoris muscle（BFM） were collected and analyzed.Results： Results showed that growing-finishing pigs fed the MP or AP diet improved（P 〈 0.01） the average daily gain and feed： gain ratio compared with those fed the LP diet, and the MP diet tended to increase（P = 0.09） the weight of LDM. Moreover, the ATP content and energy charge value were varied among muscle samples from different locations of pigs fed the reduced protein diets. We also observed that pigs fed the MP diet up-regulated（P 〈 0.05） muscular m RNA expression of all the selected key genes, except that myosin heavy chain（My HC） IIb,My HC IIx, while m RNA expression of ubiquitin ligases genes was not affected by dietary CP level. Additionally, the activation of mammalian target of rapamycin complex 1（m TORC1） pathway was stimulated（P 〈 0.05） in skeletal muscle of the pigs fed the MP or AP diet compared with those fed the LP diet.Conclusion： The results suggest that the pigs fed the MP diet could catch up to the growth performance and the LDM weight of the pigs fed the AP diet, and the underlying mechanism may be partly due to the alteration in energy status, modulation of muscle fiber characteristics and m TORC1 activation as well as its downstream effectors in skeletal muscle of different locations in growing-finishing pigs.展开更多
Previous studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In ...Previous studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.展开更多
【目的】探究罗伊氏乳杆菌CCTCC M 2016546对慢性温和不可预知应激(chronic unpredictable mild stress,CUMS)诱导的抑郁模型大鼠的抗抑郁效果及其潜在机制。【方法】将60只雄性SD大鼠随机分为空白组(n=8)、模型组(n=43)、预防组(n=9)...【目的】探究罗伊氏乳杆菌CCTCC M 2016546对慢性温和不可预知应激(chronic unpredictable mild stress,CUMS)诱导的抑郁模型大鼠的抗抑郁效果及其潜在机制。【方法】将60只雄性SD大鼠随机分为空白组(n=8)、模型组(n=43)、预防组(n=9)。除空白组外,其余大鼠每日接受3种应激刺激,预防组大鼠于每日造模前灌服罗伊氏乳杆菌CCTCC M 2016546(1×10^(9)CFU/d)。4周后,通过糖水偏好试验、强迫游泳试验、旷场试验评估大鼠抑郁状态,并根据评估结果将建模成功的大鼠随机分为模型组(n=8)、氟西汀组(2.1 mg/kg氟西汀,n=8)、协同组(2.1 mg/kg氟西汀+1×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)、高剂量组(5×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)、低剂量组(1×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)。各组大鼠每天接受1次药物治疗,持续4周。再次进行行为学测试,观察罗伊氏乳杆菌CCTCC M 2016546对抑郁模型大鼠行为学的影响。采用酶联免疫法(enzyme-linked immunosorbent assay,ELISA)检测大鼠海马组织中5-羟色胺(5-hydroxytryptamine,5-HT)及血清中促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(cortisol,CORT)的含量;采用蛋白印迹法(Western blotting)测定脑组织中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、酪氨酸激酶受体B(tyrosine kinase receptor B,TrkB)、蛋白激酶B(protein kinase B,AKT)、磷脂酰肌醇3-激酶(phosphatidylinisitol 3-kinase,PI3K)、细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)、核转录因子cAMP反应元件结合蛋白(cAMP-response element binding protein,CREB)的蛋白表达。【结果】氟西汀、协同给药及罗伊氏乳杆菌CCTCC M 2016546均能显著改善抑郁大鼠的行为表现,可增加糖水偏好(P<0.01,P<0.001),减少悬尾不动时间(P<0.01,P<0.05),提高水平运动总距离及中央区域停留时间(P<0.01,P<0.05)。此外,氟西汀和罗伊氏乳杆菌CCTCC M 2016546还能恢复CUMS引起的5-HT、CORT和ACTH水平变化(P<0.01,P<0.05)。在蛋白表达方面,氟西汀组和协同组促进了BDNF、PI3K、CREB、TrkB、Akt、ERK的表达(P<0.01,P<0.05),其中高剂量罗伊氏乳杆菌CCTCC M 2016546可增加AKT、BDNF、CREB、PI3K的蛋白水平(P<0.01,P<0.05),而低剂量则提高了AKT和BDNF水平(P<0.01),预防性给药主要改善了TrkB的表达(P<0.05)。【结论】罗伊氏乳杆菌CCTCC M 2016546对抑郁模型大鼠具有抗抑郁效果,推测其可能通过调节下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)轴功能和PI3K/AKT/CREB/BDNF信号通路发挥作用。展开更多
文摘Objective:To explore the interference of monoclonal immunoglobulin(M protein)on the detection of serum LDL-C in patients with multiple myeloma,improve the understanding of this matter,determine and establish the correct method,and provide more accurate clinical results through this case.Methods:A case was selected for analysis by the direct method.Results:The interference of IgG kappa-type M protein on LDL-C detection could not be completely eliminated by the enzymatic method.Conclusion:IgG-type type M protein affects the detection of LDL-C by the enzymatic method;thus,light reagents can be used with the direct method for detection.
文摘In this paper, we report a multiple sequence alignment result on the basis of 10 amino acid sequences of the M protein, which come from different coronaviruses (4 SARS associated and 6 others known). The alignment model was based on the profile HMM (Hidden Markov Model), and the model training was implemented through the SAHMM (Self Adapting Hidden Markov Model) software developed by the authors.
基金supported by National Natural Science Foundation of China (30771970 and 30872399)Scientific Research Foundation of Health Bureau of Hebei Province (08054).
文摘Group A streptococcus （GAS）, an important human pathogen, can cause various kinds of infections including superficial infections and potentially lethal infections, and the search for an effective vaccine to prevent GAS infections has been ongoing for many years. This paper compares the immunogenicity and immunoprotection of FbaA （an Fn-binding protein expressed on the surface of GAS） with that of M protein, the best immunogen of GAS. Assay for immune response showed that FbaA, similar to M protein, could induce protein-specific high IgG titer in BALB/c mice. Furthermore, following GAS challenge, the mice immunized with FbaA showed the same protective rate as those with M protein. These results indicate that FbaA is similar in ability to M protein in inducing protective immunity against GAS challenge in mice. Cellular ＆ Molecular Immunology.
文摘Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Arsenazo M itself. The apparent molar absorptivity of the BSA-Arsenazo M complex is 3.21105 Lmol-1cm-1. The linear ranges for protein determination are wide (at least 0-100 mg/mL).
文摘We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.
文摘目的研究肝细胞肝癌(HCC)中甲基转移酶样蛋白5(METTL5)、病毒样m^(6)A甲基转化酶(VIRMA)的表达及与临床预后的关系。方法选取2018年3月至2021年3月就诊于该院的89例HCC患者。应用免疫组化和荧光定量PCR检测组织METTL5、VIRMA蛋白和m RNA表达。利用Kaplan-Meier曲线和Cox回归分析METTL5、VIRMA蛋白对HCC预后的影响。结果HCC癌组织METTL5 m RNA(3.23±0.55 vs.0.61±0.20),VIRMA m RNA(2.97±0.42 vs.0.58±0.17)高于癌旁组织,差异有统计学意义(t=42.234、49.762,均P<0.001)。METTL5、VIRMA蛋白位于细胞质和细胞膜,部分位于细胞核。HCC癌组织METTL5[71.91%(64/89)vs.5.62%(5/89)]、VIRMA[69.66%(62/89)vs.6.74%(6/89)]阳性率高于癌旁组织,差异有统计学意义(χ^(2)=82.385、74.627,P<0.001,P=0.001)。HCC中METTL5、VIRMA蛋白表达与肝癌分期标准(CNLC)、肿瘤最大径及血管侵犯有关,CNLC分期Ⅱ~Ⅲ期、肿瘤最大径≥5 cm及血管侵犯的HCC癌组织中METTL5、VIRMA蛋白阳性率较高(均P<0.05)。METTL5阳性组和阴性组3年生存率为34.88%(15/43),67.39%(31/46),差异有统计学意义(Log-rankχ^(2)=7.893,P=0.005)。VIRMA阳性组和阴性组3年生存率为36.36%(16/44)、66.67%(30/45),差异有统计学意义(Log-rankχ^(2)=9.828,P=0.002)。CNLC分期Ⅱ~Ⅲ期、肿瘤最大径≥5 cm、血管侵犯、METTL5阳性、VIRMA阳性是影响HCC患者不良预后的危险因素。结论HCC中METTL5、VIRMA表达上调,均与CNLC分期Ⅱ~Ⅲ期、肿瘤最大径及血管侵犯有关,是评估HCC预后的肿瘤标志物。
基金Supported by the National Natural Science Foundation of China,No.61802350
文摘BACKGROUND Studies have shown that insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)plays critical roles in the genesis and development of human cancers.AIM To investigate the clinical significance and role of IGF2BP1 in pancreatic cancer.METHODS Expression levels of IGF2BP1 and microRNA-494(miR-494)were mined based on Gene Expression Omnibus datasets and validated in both clinical samples and cell lines by quantitative real-time polymerase chain reaction and Western blot.The relationship between IGF2BP1 expression and clinicopathological factors of pancreatic cancer patients was analyzed.The effect and mechanism of IGF2BP1 on pancreatic cancer cell proliferation were investigated in vitro and in vivo.Analyses were performed to explore underlying mechanisms of IGF2BP1 upregulation in pancreatic cancer and assays were carried out to verify the posttranscriptional regulation of IGF2BP1 by miR-494.RESULTS We found that IGF2BP1 was upregulated and associated with a poor prognosis in pancreatic cancer patients.We showed that downregulation of IGF2BP1 inhibited pancreatic cancer cell growth in vitro and in vivo via the AKT signaling pathway.Mechanistically,we showed that the frequent upregulation of IGF2BP1 was attributed to the downregulation of miR-494 expression in pancreatic cancer.Furthermore,we discovered that reexpression of miR-494 could partially abrogate the oncogenic role of IGF2BP1.CONCLUSION Our results revealed that upregulated IGF2BP1 promotes the proliferation of pancreatic cancer cells via the AKT signaling pathway and confirmed that the activation of IGF2BP1 is partly due to the silencing of miR-494.
基金financially supported by the National Basic Research Program of China(2013CB127305)the Nature Science Foundation of Hunan Province(S2014J504I)+1 种基金the Major Project of Hunan Province(2015NK1002)the National Science and Technology Ministry(2014BAD08B11)
文摘Background： To investigate the effects of dietary crude protein（CP） restriction on muscle fiber characteristics and key regulators related to protein deposition in skeletal muscle, a total of 18 growing-finishing pigs（62.30 ± 0.88 kg）were allotted to 3 groups and fed with the recommended adequate protein（AP, 16 % CP） diet, moderately restricted protein（MP, 13 % CP） diet and low protein（LP, 10 % CP） diet, respectively. The skeletal muscle of different locations in pigs, including longissimus dorsi muscle（LDM）, psoas major muscle（PMM） and biceps femoris muscle（BFM） were collected and analyzed.Results： Results showed that growing-finishing pigs fed the MP or AP diet improved（P 〈 0.01） the average daily gain and feed： gain ratio compared with those fed the LP diet, and the MP diet tended to increase（P = 0.09） the weight of LDM. Moreover, the ATP content and energy charge value were varied among muscle samples from different locations of pigs fed the reduced protein diets. We also observed that pigs fed the MP diet up-regulated（P 〈 0.05） muscular m RNA expression of all the selected key genes, except that myosin heavy chain（My HC） IIb,My HC IIx, while m RNA expression of ubiquitin ligases genes was not affected by dietary CP level. Additionally, the activation of mammalian target of rapamycin complex 1（m TORC1） pathway was stimulated（P 〈 0.05） in skeletal muscle of the pigs fed the MP or AP diet compared with those fed the LP diet.Conclusion： The results suggest that the pigs fed the MP diet could catch up to the growth performance and the LDM weight of the pigs fed the AP diet, and the underlying mechanism may be partly due to the alteration in energy status, modulation of muscle fiber characteristics and m TORC1 activation as well as its downstream effectors in skeletal muscle of different locations in growing-finishing pigs.
基金supported by the National Natural Science Foundation of China,No.81350013,30872609
文摘Previous studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
文摘【目的】探究罗伊氏乳杆菌CCTCC M 2016546对慢性温和不可预知应激(chronic unpredictable mild stress,CUMS)诱导的抑郁模型大鼠的抗抑郁效果及其潜在机制。【方法】将60只雄性SD大鼠随机分为空白组(n=8)、模型组(n=43)、预防组(n=9)。除空白组外,其余大鼠每日接受3种应激刺激,预防组大鼠于每日造模前灌服罗伊氏乳杆菌CCTCC M 2016546(1×10^(9)CFU/d)。4周后,通过糖水偏好试验、强迫游泳试验、旷场试验评估大鼠抑郁状态,并根据评估结果将建模成功的大鼠随机分为模型组(n=8)、氟西汀组(2.1 mg/kg氟西汀,n=8)、协同组(2.1 mg/kg氟西汀+1×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)、高剂量组(5×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)、低剂量组(1×10^(9)CFU/d罗伊氏乳杆菌CCTCC M 2016546,n=9)。各组大鼠每天接受1次药物治疗,持续4周。再次进行行为学测试,观察罗伊氏乳杆菌CCTCC M 2016546对抑郁模型大鼠行为学的影响。采用酶联免疫法(enzyme-linked immunosorbent assay,ELISA)检测大鼠海马组织中5-羟色胺(5-hydroxytryptamine,5-HT)及血清中促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(cortisol,CORT)的含量;采用蛋白印迹法(Western blotting)测定脑组织中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、酪氨酸激酶受体B(tyrosine kinase receptor B,TrkB)、蛋白激酶B(protein kinase B,AKT)、磷脂酰肌醇3-激酶(phosphatidylinisitol 3-kinase,PI3K)、细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)、核转录因子cAMP反应元件结合蛋白(cAMP-response element binding protein,CREB)的蛋白表达。【结果】氟西汀、协同给药及罗伊氏乳杆菌CCTCC M 2016546均能显著改善抑郁大鼠的行为表现,可增加糖水偏好(P<0.01,P<0.001),减少悬尾不动时间(P<0.01,P<0.05),提高水平运动总距离及中央区域停留时间(P<0.01,P<0.05)。此外,氟西汀和罗伊氏乳杆菌CCTCC M 2016546还能恢复CUMS引起的5-HT、CORT和ACTH水平变化(P<0.01,P<0.05)。在蛋白表达方面,氟西汀组和协同组促进了BDNF、PI3K、CREB、TrkB、Akt、ERK的表达(P<0.01,P<0.05),其中高剂量罗伊氏乳杆菌CCTCC M 2016546可增加AKT、BDNF、CREB、PI3K的蛋白水平(P<0.01,P<0.05),而低剂量则提高了AKT和BDNF水平(P<0.01),预防性给药主要改善了TrkB的表达(P<0.05)。【结论】罗伊氏乳杆菌CCTCC M 2016546对抑郁模型大鼠具有抗抑郁效果,推测其可能通过调节下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)轴功能和PI3K/AKT/CREB/BDNF信号通路发挥作用。
