Fungal pathogens are among the main destructive microorganisms for crops and ecosystems worldwide,causing substantial agricultural and economic losses.Plant cell surface-localized lysin motif(LysM)-containing receptor...Fungal pathogens are among the main destructive microorganisms for crops and ecosystems worldwide,causing substantial agricultural and economic losses.Plant cell surface-localized lysin motif(LysM)-containing receptor-like kinases(RLKs)or receptor-like proteins(RLPs)enhance plant resistance to fungal pathogens via sensing chitin,which is a conserved component of the fungal cell wall.Other types of RLKs also regulate chitin signaling via distinct mechanisms in plants.In this study,we identified a G-type lectin RLK,NbERK1,which positively regulated chitin signaling and resistance to the fungal pathogen Sclerotinia sclerotiorum in the model plant Nicotiana benthamiana.In addition,the LysM-RLK NbCERK1/NbLYK4 was shown to mediate plant resistance to S.sclerotiorum positively.Further,the association of chitin-induced NbCERK1-NbLYK4 was found to be essential for chitin perception and signaling.Importantly,NbERK1 was associated with NbCERK1/NbLYK4 and positively regulated chitin-induced NbCERK1-NbLYK4 association.Moreover,chitin could induce the dissociation of NbERK1 from the NbCERK1-NbLYK4 complex.Also,the kinase activity of NbERK1 was likely essential for this dissociation and plant resistance-enhancing activity of NbERK1.Together,these results suggest that NbERK1 is a novel component of the chitin receptor complex and enhances plant resistance to fungal pathogens via regulating chitin signaling.展开更多
基金supported by the National Natural Science Foundation of China(32100155)the Natural Science Foundation of Jiangsu Province(BK20221000)+1 种基金the Fundamental Research Funds for the Central Universities(KYLH201703)the Jiangsu Funding Program for Excellent Postdoctoral Talent(2022ZB343).
文摘Fungal pathogens are among the main destructive microorganisms for crops and ecosystems worldwide,causing substantial agricultural and economic losses.Plant cell surface-localized lysin motif(LysM)-containing receptor-like kinases(RLKs)or receptor-like proteins(RLPs)enhance plant resistance to fungal pathogens via sensing chitin,which is a conserved component of the fungal cell wall.Other types of RLKs also regulate chitin signaling via distinct mechanisms in plants.In this study,we identified a G-type lectin RLK,NbERK1,which positively regulated chitin signaling and resistance to the fungal pathogen Sclerotinia sclerotiorum in the model plant Nicotiana benthamiana.In addition,the LysM-RLK NbCERK1/NbLYK4 was shown to mediate plant resistance to S.sclerotiorum positively.Further,the association of chitin-induced NbCERK1-NbLYK4 was found to be essential for chitin perception and signaling.Importantly,NbERK1 was associated with NbCERK1/NbLYK4 and positively regulated chitin-induced NbCERK1-NbLYK4 association.Moreover,chitin could induce the dissociation of NbERK1 from the NbCERK1-NbLYK4 complex.Also,the kinase activity of NbERK1 was likely essential for this dissociation and plant resistance-enhancing activity of NbERK1.Together,these results suggest that NbERK1 is a novel component of the chitin receptor complex and enhances plant resistance to fungal pathogens via regulating chitin signaling.
