Photochemical aging of volatile organic compounds(VOCs)in the atmosphere is an important source of secondary organic aerosol(SOA).To evaluate the formation potential of SOA at an urban site in Lyon(France),an outdoor ...Photochemical aging of volatile organic compounds(VOCs)in the atmosphere is an important source of secondary organic aerosol(SOA).To evaluate the formation potential of SOA at an urban site in Lyon(France),an outdoor experiment using a Potential Aerosol Mass(PAM)oxidation flow reactor(OFR)was conducted throughout entire days during JanuaryFebruary 2017.Diurnal variation of SOA formations and their correlation with OH radical exposure(OHexp),ambient pollutants(VOCs and particulate matters,PM),Relative Humidity(RH),and temperature were explored in this study.Ambient urban air was exposed to high concentration of OH radicals with OHexp in range of(0.2-1.2)×10^12 molecule/(cm^3·sec),corresponding to several days to weeks of equivalent atmospheric photochemical aging.The results informed that urban air at Lyon has high potency to contribute to SOA,and these SOA productions were favored from OH radical photochemical oxidation rather than via ozonolysis.Maximum SOA formation(36μg/m^3)was obtained at OHexp of about 7.4×10^11 molecule/(cm^3·sec),equivalent to approximately 5 days of atmospheric oxidation.The correlation between SOA formation and ambient environment conditions(RH&temperature,VOCs and PM)was observed.It was the first time to estimate SOA formation potential from ambient air over a long period in urban environment of Lyon.展开更多
Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结...Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结果显示:该检测方法的Ct值与标准品模板线性关系良好,相关系数为0.9989;利用该方法检测CBoV、FBoV、AGV2这几种病毒的基因组均没有出现非特异性扩增;敏感性比普通PCR检测方法高100倍;组内和组间重复性试验的变异系数小于1%;通过60份样本检测发现LIPyV阳性率为6.67%,比普通PCR更灵敏可靠。结果表明,本试验建立的SYBR Green I实时荧光定量PCR方法可用于LIPyV的快速检测。展开更多
Clinical practice guidelines drive clinical practice and clinicians rely to them when trying to answer their most common questions.One of the most important position papers in the field of gastro-esophageal reflux dis...Clinical practice guidelines drive clinical practice and clinicians rely to them when trying to answer their most common questions.One of the most important position papers in the field of gastro-esophageal reflux disease(GERD)is the one produced by the Lyon Consensus.Recently an updated second version has been released.Mean nocturnal baseline impedance(MNBI)was proposed by the first Consensus to act as supportive evidence for GERD diagnosis.Originally a cut-off of 2292 Ohms was proposed,a value revised in the second edition.The updated Consensus recommended that an MNBI<1500 Ohms strongly suggests GERD while a value>2500 Ohms can be used to refute GERD.The proposed cut-offs move in the correct direction by diminishing the original cut-off,nevertheless they arise from a study of normal subjects where cut-offs were provided by measuring the mean value±2SD and not in symptomatic patients.However,data exist that even symptomatic patients with inconclusive disease or reflux hypersensitivity(RH)show lower MNBI values in comparison to normal subjects or patients with functional heartburn(FH).Moreover,according to the data,MNBI,even among symptomatic patients,is affected by age and body mass index.Also,various studies have proposed different cut-offs by using receiver operating characteristic curve analysis even lower than the one proposed.Finally,no information is given for patients submitted to on-proton pump inhibitors pH-impedance studies even if new and extremely important data now exist.Therefore,even if MNBI is an extremely important tool when trying to approach patients with reflux symptoms and could distinguish conclusive GERD from RH or FH,its values should be interpreted with caution.展开更多
Background and Aims:Recently,Lyon Consensus 2.0 recommended Los Angeles(LA)-B esophagitis as conclusive evidence and LAA esophagitis as borderline evidence for gastroesophageal reflux disease(GERD).This study aimed to...Background and Aims:Recently,Lyon Consensus 2.0 recommended Los Angeles(LA)-B esophagitis as conclusive evidence and LAA esophagitis as borderline evidence for gastroesophageal reflux disease(GERD).This study aimed to investigate the diagnostic value of LA-B and LA-A esophagitis.Methods:Patients with typical reflux symptoms who underwent endoscopy examination and received acid-suppressive therapy from two tertiary hospitals[the First Affiliated Hospital of Sun Yat-sen University(Guangzhou,P.R.China)and the Third People’s Hospital of Chengdu(Chengdu,P.R.China)]were retrospectively included.Acid-suppression response rates,endoscopy results,motility,and reflux parameters were compared between patients with different grades of esophagitis.Results:In total,401 patients were enrolled,among whom 254 were without reflux esophagitis(RE),51 had LA-A esophagitis,44 had LA-B esophagitis,and 52 had LA-C/D esophagitis.Patients with LA-B esophagitis and LA-C/D esophagitis had significantly higher acid-suppressive response rates than non-RE patients(P<0.05),whereas no significant difference was found between patients with LA-A esophagitis and non-RE patients(non-RE vs LA-A vs LA-B vs LA-C/D:52.4%vs 70.6%vs 75.0%vs 82.7%).Among patients with LA-A esophagitis,those with a number of reflux episodes that exceeded 80 per day(90.0%vs 52.4%,P=0.044)or hypotensive esophagogastric junction(72.4%vs 52.4%,P=0.040)had significantly higher acid-suppressive response rates than non-RE patients.Conclusions:LA-B esophagitis can be regarded as conclusive evidence for GERD and initiate acid-suppressive therapy.LA-A esophagitis did not establish a definite GERD diagnosis alone.When combined with adjunctive or supportive evidence,the acid-suppressive therapy response rate of LA-A esophagitis improved.展开更多
基金the Institute for Research on Catalysis and the Environment of Lyon(IRCELYON)supported by the"Investissement d’Avenir"PEPS Program Project(ASTRAL)of the University of Lyon and French National center for Scientific Research(French:center national de la recherche scientifique,CNRS)as part of the ANR-11-IDEX-0007 programby the European Research Council under the Horizon 2020 Research and Innovation Program Project of the European Union under Convention N°690958(MARSU)。
文摘Photochemical aging of volatile organic compounds(VOCs)in the atmosphere is an important source of secondary organic aerosol(SOA).To evaluate the formation potential of SOA at an urban site in Lyon(France),an outdoor experiment using a Potential Aerosol Mass(PAM)oxidation flow reactor(OFR)was conducted throughout entire days during JanuaryFebruary 2017.Diurnal variation of SOA formations and their correlation with OH radical exposure(OHexp),ambient pollutants(VOCs and particulate matters,PM),Relative Humidity(RH),and temperature were explored in this study.Ambient urban air was exposed to high concentration of OH radicals with OHexp in range of(0.2-1.2)×10^12 molecule/(cm^3·sec),corresponding to several days to weeks of equivalent atmospheric photochemical aging.The results informed that urban air at Lyon has high potency to contribute to SOA,and these SOA productions were favored from OH radical photochemical oxidation rather than via ozonolysis.Maximum SOA formation(36μg/m^3)was obtained at OHexp of about 7.4×10^11 molecule/(cm^3·sec),equivalent to approximately 5 days of atmospheric oxidation.The correlation between SOA formation and ambient environment conditions(RH&temperature,VOCs and PM)was observed.It was the first time to estimate SOA formation potential from ambient air over a long period in urban environment of Lyon.
文摘Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结果显示:该检测方法的Ct值与标准品模板线性关系良好,相关系数为0.9989;利用该方法检测CBoV、FBoV、AGV2这几种病毒的基因组均没有出现非特异性扩增;敏感性比普通PCR检测方法高100倍;组内和组间重复性试验的变异系数小于1%;通过60份样本检测发现LIPyV阳性率为6.67%,比普通PCR更灵敏可靠。结果表明,本试验建立的SYBR Green I实时荧光定量PCR方法可用于LIPyV的快速检测。
文摘Clinical practice guidelines drive clinical practice and clinicians rely to them when trying to answer their most common questions.One of the most important position papers in the field of gastro-esophageal reflux disease(GERD)is the one produced by the Lyon Consensus.Recently an updated second version has been released.Mean nocturnal baseline impedance(MNBI)was proposed by the first Consensus to act as supportive evidence for GERD diagnosis.Originally a cut-off of 2292 Ohms was proposed,a value revised in the second edition.The updated Consensus recommended that an MNBI<1500 Ohms strongly suggests GERD while a value>2500 Ohms can be used to refute GERD.The proposed cut-offs move in the correct direction by diminishing the original cut-off,nevertheless they arise from a study of normal subjects where cut-offs were provided by measuring the mean value±2SD and not in symptomatic patients.However,data exist that even symptomatic patients with inconclusive disease or reflux hypersensitivity(RH)show lower MNBI values in comparison to normal subjects or patients with functional heartburn(FH).Moreover,according to the data,MNBI,even among symptomatic patients,is affected by age and body mass index.Also,various studies have proposed different cut-offs by using receiver operating characteristic curve analysis even lower than the one proposed.Finally,no information is given for patients submitted to on-proton pump inhibitors pH-impedance studies even if new and extremely important data now exist.Therefore,even if MNBI is an extremely important tool when trying to approach patients with reflux symptoms and could distinguish conclusive GERD from RH or FH,its values should be interpreted with caution.
基金supported by the China Postdoctoral Science Foundation[grant number 2024M763808]the Postdoctoral Fellowship Program of CPSF[grant number GZB20240889]+1 种基金the China National Natural Science Foundation[grant number 82300647]the Guangzhou Nansha District livelihood science and technology plan[grant number KNS0301005].
文摘Background and Aims:Recently,Lyon Consensus 2.0 recommended Los Angeles(LA)-B esophagitis as conclusive evidence and LAA esophagitis as borderline evidence for gastroesophageal reflux disease(GERD).This study aimed to investigate the diagnostic value of LA-B and LA-A esophagitis.Methods:Patients with typical reflux symptoms who underwent endoscopy examination and received acid-suppressive therapy from two tertiary hospitals[the First Affiliated Hospital of Sun Yat-sen University(Guangzhou,P.R.China)and the Third People’s Hospital of Chengdu(Chengdu,P.R.China)]were retrospectively included.Acid-suppression response rates,endoscopy results,motility,and reflux parameters were compared between patients with different grades of esophagitis.Results:In total,401 patients were enrolled,among whom 254 were without reflux esophagitis(RE),51 had LA-A esophagitis,44 had LA-B esophagitis,and 52 had LA-C/D esophagitis.Patients with LA-B esophagitis and LA-C/D esophagitis had significantly higher acid-suppressive response rates than non-RE patients(P<0.05),whereas no significant difference was found between patients with LA-A esophagitis and non-RE patients(non-RE vs LA-A vs LA-B vs LA-C/D:52.4%vs 70.6%vs 75.0%vs 82.7%).Among patients with LA-A esophagitis,those with a number of reflux episodes that exceeded 80 per day(90.0%vs 52.4%,P=0.044)or hypotensive esophagogastric junction(72.4%vs 52.4%,P=0.040)had significantly higher acid-suppressive response rates than non-RE patients.Conclusions:LA-B esophagitis can be regarded as conclusive evidence for GERD and initiate acid-suppressive therapy.LA-A esophagitis did not establish a definite GERD diagnosis alone.When combined with adjunctive or supportive evidence,the acid-suppressive therapy response rate of LA-A esophagitis improved.
