Heat and acid treatments were reported to be a promising substitute for SO2 fumigation in color protection of postharvest lychee （Litchi chinensis） fruits, but the mechanism was not clear. In the present study, hot ...Heat and acid treatments were reported to be a promising substitute for SO2 fumigation in color protection of postharvest lychee （Litchi chinensis） fruits, but the mechanism was not clear. In the present study, hot water （70℃） dipping followed by immersion in 2% HC1 （heat-acid） substantially protected the red color of the fruit during storage at 25℃ and inhibited anthocyanin degradation while hot water dipping alone （heat） led to rapidly browning and about 90% loss in anthocyanin content. The pH values in the pericarp of the heat-acid treated fruit dropped to 3.2, while the values maintained around 5.0 in the heat-treated and control fruit. No significantly different pH values were detected among the arils of heat-acid, heat treated and control fruit. Heat-acid treatment dramatically reduced the activities of anthocyanin degradation enzyme （ADE）, peroxidase （POD） and polyphenol oxidase in the pericarp. A marked reduction in LcPOD gene expression was also detected in heat-acid treated fruit, in contrast, induction was found in heat treated fruit. The pericarp of heat-acid treated fruit exhibited significantly lower respiration rate but faster water loss than that of the untreated or heat treated fruit. Taken together, heat treatment triggered quick browning and anthocyanin loss in lychee fruit, while heat-acid treatment protected the fruit color by a great reduction in the activities/gene expression of anthocyanin degradation enzymes and acidification of lychee pericarp.展开更多
OBJECTIVE Lychee seed,a famous traditional Chinese medicine,recently were reported to improve the learning and memory abilities in mice.However,it is still unclear whether lychee seed saponins(LSS)can improve the cogn...OBJECTIVE Lychee seed,a famous traditional Chinese medicine,recently were reported to improve the learning and memory abilities in mice.However,it is still unclear whether lychee seed saponins(LSS)can improve the cognitive function and associated mechanisms.METHODS In present studies,we established the Alzheimer disease(AD)model by injecting Aβ25-35 into the lateral ventricle of rats.Then the spatial learning and memory abilities of LSS-treated rats were evaluated with the Morris water maze,meanwhile the protein expressions of AKT,GSK3β and Tau in the hippocampal neuron were analyzed by immunohistochemistry and Western blotting.RESULTS The results showed LSS can improve the cognitive functions of AD rats through shortening the escape latency,increasing the number across the platform,platform quadrant dwell time and the percentage of the total distance run platform quadrant.The protein expression of AKT was significantly up-regulated and that of GSK3β and Tau were decreased remarkably in the hippocampal CA1 area.CONCLUSION Our study is the first to show that LSS significantly improve the cognitive function and prevent hippocampal neuronal injury of the rats with AD by activation of the PI3K/AKT/GSK3βsignaling pathway,suggesting LSS may be developed into the nutrient supplement for the treatment of AD.展开更多
The aim was to optimize ultrasonic extraction conditions and study antioxidant activities of lychee pericarp polysaccharides. The effects of extraction temperature,time,ultrasonic power,solid-liquid ratio on polysacch...The aim was to optimize ultrasonic extraction conditions and study antioxidant activities of lychee pericarp polysaccharides. The effects of extraction temperature,time,ultrasonic power,solid-liquid ratio on polysaccharides yield were investigated by single-factor experiment and orthogonal experiment to obtain optimum extraction conditions; the antioxidant activities of lychee pericarp polysaccharides were evaluated by the total antioxidant activity. Optimum extraction process was under extraction temperature of 80 ℃,extraction time of 2. 5 h,ultrasonic power of 180 W,solid-liquid ratio of 1∶ 25 g / m L; the total antioxidant activity was increased with the increasing concentration of polysaccharides. The extraction process is simple and efficient. The extraction rate of lychee pericarp polysaccharides with antioxidant activities is high.展开更多
Through field experiment,we explore the impact of long-term drip fertigation on growth and distribution of lychee root and changes of soil pH in different layers of soil in lychee garden.The results show that drip fer...Through field experiment,we explore the impact of long-term drip fertigation on growth and distribution of lychee root and changes of soil pH in different layers of soil in lychee garden.The results show that drip fertigation can significantly promote the growth of lychee roots,and increase the contact area of root and soil;if it experiences six years of drip fertigation successively,the dry weight of root,root length and surface area of root in soil in drip fertigation area,will be 2.29 times,2.17 times and 2.25 times that in non-drip fertigation area,respectively.The lychee root is mainly distributed in 0-40 cm layer of soil,but there is conspicuous difference between drip fertigation area and non-drip fertigation area in terms of root distribution in 0-20 cm and 20-40 cm layer of soil.Drip fertigation is more favorable for the root to go deep inside the soil.Under long-term drip fertigation,the soil acidification in lychee garden is prominent,and in comparison with non-drip fertigation area,there is the greatest decline in soil pH in 10-20 cm layer of soil in drip fertigation area,reaching 1.47 units.展开更多
Background Lychee seeds were fermented by three kinds of bacteria(Lactobacillus plantarum,Saccharomyces cer-evillus and ganoderma lucidum mycelium),and two effective strains were selected by two indexes of activity co...Background Lychee seeds were fermented by three kinds of bacteria(Lactobacillus plantarum,Saccharomyces cer-evillus and ganoderma lucidum mycelium),and two effective strains were selected by two indexes of activity content and antioxidant,so as to further verify whether lychee seeds have waste multiplication effect and can protect cells damaged by oxidation from anti-inflammatory,anti-aging and safety.Results The contents of polyphenols,flavonoids and proteins in the solution fermented by Ganoderma lucidum mycelium did not increase,thus affecting the antioxidant capacity of the solution was far less than that of the water extract.The active content of the other two fermentation solutions was higher than that of the water extract,and the ability of scavenging free radicals of the two solutions increased with the increase of the volume fraction.At the cellular level,the two fermentation solutions showed repair effects on UVA-induced damaged cells.The contents of type I collagen(COL-1),total antioxidant capacity and ELN were increased,the contents of reactive oxygen species and MDA were decreased,and the expressions of inflammatory factors IL-6,TNF-a,iNOS and COX-2 were decreased in HaCaT cells.From the gene level,the mRNA contents of IL-6,TNF-a,Caspase-3,Caspase-9,Bax and Bcl were significantly decreased.The test of chick embryo chorioallantoic membrane(HTET CAM)showed that there was no bleeding and litchi seed fermentation liquid was not irritating.Conclusions Therefore,two kinds of litchi seed fermentation can be used as natural plant raw materials for cosmetics,and have strong antioxidant,anti-inflammatory and anti-aging functions on skin,and also have good human safety.展开更多
Juice drinks are an important commercialization alternative for lychee, a tropical and subtropical fruit. Although the lychee juice content is important when assessing the quality of a drink, there are no published me...Juice drinks are an important commercialization alternative for lychee, a tropical and subtropical fruit. Although the lychee juice content is important when assessing the quality of a drink, there are no published methods to determine it, particularly simple ones for the routine inspection of juice drinks. Lychee juice drinks contain ingredients with buffering capacity including proteins and ions such as phosphate, citrate, lactate, carbonate, acetate and propionate. The relationship between their buffering capacity and lychee juice content was studied. Citric acid was added to pure lychee dilutions in distilled water containing 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20% and 10% lychee juice. The pH of the dilutions was measured to obtain a linear model for the molar H+ concentration as a function of the added citric acid (g/L) amount LC = (BC-494.2)/12,031, where LC was the lychee juice content and BC was defined as the juice buffering coefficient.展开更多
Roberts has divided seeds into orthodox seeds and recalcitrant seeds according to their storage behaviours. The former can tolerate desiccation and freezing temperatures, whereas the latter are susceptible to the two ...Roberts has divided seeds into orthodox seeds and recalcitrant seeds according to their storage behaviours. The former can tolerate desiccation and freezing temperatures, whereas the latter are susceptible to the two factors. When their water contents are lower than cer-展开更多
Micrographs of lychee pericarp and pulp during microwave vacuum drying were tested and analyzed in order to illuminate the microstructure change of lychee and effect of the change on moisture removing in lychee.The re...Micrographs of lychee pericarp and pulp during microwave vacuum drying were tested and analyzed in order to illuminate the microstructure change of lychee and effect of the change on moisture removing in lychee.The results showed that the pericarp consisted of three parts:outer layer with cuticle,inter layer and inner layer.Outer layer and inter layer cells are easily destroyed than inner layer because of small and intact inner layer cells.Furthermore,micrographs showed that the moisture content of pulp keep constant with the temperature increasing at first 40 min due to the inner layer cells prevent the moisture removing from pulp.The long tubular structure of pulp cell would become break and lost over time,because the intercellular spaces reduced and the moisture removing was slow down in pulp.Meanwhile,the microstructure of lychee dried with temperature control was better than that without temperature control.展开更多
Lychee is one of the famous fruits in the subtropical regions. However, lychee seedsdo not undergo maturation desiccation during development, and cannot tolerate drying andchilling in storage. Under natural conditions...Lychee is one of the famous fruits in the subtropical regions. However, lychee seedsdo not undergo maturation desiccation during development, and cannot tolerate drying andchilling in storage. Under natural conditions, lychee seeds can only maintain viability forseveral days, and their germplasm conservation is rather difficult using conventional meth-ods. So they are called recalcitrant seeds.展开更多
Acetic acid is the main component of the volatile acid in the wine.However,excessive amounts of acetic acid negatively affect wine quality.The study aimed to decrease acetic acid content produced by Saccharomyces cere...Acetic acid is the main component of the volatile acid in the wine.However,excessive amounts of acetic acid negatively affect wine quality.The study aimed to decrease acetic acid content produced by Saccharomyces cerevisiae fermentation after adding metal ion at different temperatures.Response surface methodology(RSM)was used to predict the optimum conditions for acetic acid removal.A central composite design was employed for the experiments and results were analyzed to obtain the best possible combination of fermentation temperature(X1:16℃-24℃)and concentrations of potassium(X2:0-12.0 mM),magnesium(X3:0-8.0 mM),and calcium ions(X4:0-0.2 mM)that would generate the minimum acetic acid in lychee wine at an initial acetic acid concentration of 1.5 g/L.Experimental data were fitted to a second-order polynomial equation using multiple regression analysis and analyzed using analysis of variance(ANOVA).During fermentation under pre-established conditions,the correlation coefficients R2 and Adj-R2 of the models for acetic acid removal were 0.9487 and 0.9007,respectively.After testing,the optimum conditions for acetic acid removal were determined as follows:fermentation temperature of 20℃;potassium,magnesium,and calcium ion concentrations of 10.1 mM,6.1 mM,and 0.2 mM,respectively.The experimental acetic acid content of lychee wine under optimal conditions was found to be 0.309 g/L,which agreed well with the model-predicted value of 0.314 g/L.展开更多
Lychee pulp phenolic extract (LPPE) possessed protective effects against nonalcoholic fatty liver disease (NAFLD), while the specific components concerned were unclear. In this study, twelve major components in LPPE w...Lychee pulp phenolic extract (LPPE) possessed protective effects against nonalcoholic fatty liver disease (NAFLD), while the specific components concerned were unclear. In this study, twelve major components in LPPE were identified by LC-MS and prepared by on line knockout methods. Two antioxidant methods companied with hepatocyte steatosis assay were used to evaluate the contribution of twelve components to the hepatoprotective effects of LPPE. The identified components include four procyanidins and eight flavonoids, they all play active roles in the oxygen radical absorbance capacity of LPPE, while only B-type procyanidin trimer and quercetin 3-O -rutinoside-7-O -rhamnoside exert positive effects on cellular antioxidant activity of LPPE. Six components including procyanidin B2, epicatechin, two quercetin glycosides, and two procyanidin trimers are considered as contributors to lipid accumulation inhibitory effects of LPPE with B-type procyanidin trimer being identified as the main contributor. All the twelve components were recognized as hepatoprotective components against NAFLD in LPPE.展开更多
In a bustling square in Guangzhou’s southern Haizhu district,a group of young activists are performing a play about a local farmer’s struggle after a failed lychee harvest.Nearby,an art exhibit documents how the sal...In a bustling square in Guangzhou’s southern Haizhu district,a group of young activists are performing a play about a local farmer’s struggle after a failed lychee harvest.Nearby,an art exhibit documents how the sale of lychees in local markets,once a common sight,is becoming increasingly rare.Both events unfolded at the Canton Harvest Festival organized by local NGOs specializing in urban-rural development on December 1,2024.展开更多
The timing is almost poetic.As lychees ripened in orchards this season,the online drama Lychee in Chang'an fickered onto screens-reawakening an ancient tale of obsession.The series resurrects Tang Dynasty(618-907)...The timing is almost poetic.As lychees ripened in orchards this season,the online drama Lychee in Chang'an fickered onto screens-reawakening an ancient tale of obsession.The series resurrects Tang Dynasty(618-907)couriers who thundered down imperial roads day and night,racing against time to deliver Guangdong's precious tropical fruit to the emperor's consort in Xi'an.For centuries。展开更多
The litchi is a fruit essential for the economies of several Southeast Asian countries,but markets regularly reject it,mainly due to spoilage.Drying extends the shelf-life of litchis,but in Thailand the optimal charac...The litchi is a fruit essential for the economies of several Southeast Asian countries,but markets regularly reject it,mainly due to spoilage.Drying extends the shelf-life of litchis,but in Thailand the optimal characteristics of the dried product have not yet been determined.The purpose of this study was to determine the optimum physicochemical properties of dried litchis-those suitable for Thai consumers.The dried fruits were submitted to physicochemical measurement and consumer evaluation,with datasets subsequently integrated using circular ideal-point regression analysis.Response surface methodology was then used to predict the optimum physicochemical properties of the fruits.It was found that Thai consumer preferences with regard to dried litchis are for the fruits to be of golden-yellow color(L*ranging around 54;H ranging around 79°),to have a soft flesh(SMF ranging between 13 and 14 kN/100 g)and to have a sweet taste(TSS:TA ranging between 25 and 28).The results may be used in the future to prescribe pretreatments and drying conditions.展开更多
Many species of Sapindaceae,such as lychee,longan,and rambutan,provide nutritious and delicious fruit.Understanding the molecular genetic mechanisms that underlie the regulation of flowering is essential for securing ...Many species of Sapindaceae,such as lychee,longan,and rambutan,provide nutritious and delicious fruit.Understanding the molecular genetic mechanisms that underlie the regulation of flowering is essential for securing flower and fruit productivity.Most endogenous and exogenous flowering cues are integrated into the florigen encoded by FLOWER-ING LOCUS T.However,the regulatory mechanisms of flowering remain poorly understood in Sapindaceae.Here,we identified 60 phosphatidylethanolamine-binding protein-coding genes from six Sapindaceae plants.Gene duplication events led to the emergence of two or more paralogs of the FT gene that have evolved antagonistic functions in Sapindaceae.Among them,the FT1-like genes are functionally conserved and promote flowering,while the FT2-like genes likely serve as repressors that delay flowering.Importantly,we show here that the natural variation at nucleotide position-1437 of the lychee FT1 promoter determined the binding affinity of the SVP protein(LcSVP9),which was a negative regulator of flowering,resulting in the differential expression of LcFT1,which in turn affected flowering time in lychee.This finding provides a potential molecular marker for breeding lychee.Taken together,our results reveal some crucial aspects of FT gene family genetics that underlie the regulation of flowering in Sapindaceae.展开更多
基金supported by the National Key Basic Research Program of China (2013CB127105)the National Natural Science Foundation of China (30671466)+1 种基金China Litchi and Logan Research System (CARS-33-14)Guangdong Fruit Research System,China (2009-356)
文摘Heat and acid treatments were reported to be a promising substitute for SO2 fumigation in color protection of postharvest lychee （Litchi chinensis） fruits, but the mechanism was not clear. In the present study, hot water （70℃） dipping followed by immersion in 2% HC1 （heat-acid） substantially protected the red color of the fruit during storage at 25℃ and inhibited anthocyanin degradation while hot water dipping alone （heat） led to rapidly browning and about 90% loss in anthocyanin content. The pH values in the pericarp of the heat-acid treated fruit dropped to 3.2, while the values maintained around 5.0 in the heat-treated and control fruit. No significantly different pH values were detected among the arils of heat-acid, heat treated and control fruit. Heat-acid treatment dramatically reduced the activities of anthocyanin degradation enzyme （ADE）, peroxidase （POD） and polyphenol oxidase in the pericarp. A marked reduction in LcPOD gene expression was also detected in heat-acid treated fruit, in contrast, induction was found in heat treated fruit. The pericarp of heat-acid treated fruit exhibited significantly lower respiration rate but faster water loss than that of the untreated or heat treated fruit. Taken together, heat treatment triggered quick browning and anthocyanin loss in lychee fruit, while heat-acid treatment protected the fruit color by a great reduction in the activities/gene expression of anthocyanin degradation enzymes and acidification of lychee pericarp.
基金supported by Science and Technology Planning Project of Sichuan Province(2008SZ0050,14JC0798)Educational Commission of Sichuan Province(10ZA035,15ZA0155)+1 种基金Science and Technology Program of Luzhou(2015-S-43,2016LZXNYD-T03)Key Development Program of Southwest Medical University(2010ZD-010)
文摘OBJECTIVE Lychee seed,a famous traditional Chinese medicine,recently were reported to improve the learning and memory abilities in mice.However,it is still unclear whether lychee seed saponins(LSS)can improve the cognitive function and associated mechanisms.METHODS In present studies,we established the Alzheimer disease(AD)model by injecting Aβ25-35 into the lateral ventricle of rats.Then the spatial learning and memory abilities of LSS-treated rats were evaluated with the Morris water maze,meanwhile the protein expressions of AKT,GSK3β and Tau in the hippocampal neuron were analyzed by immunohistochemistry and Western blotting.RESULTS The results showed LSS can improve the cognitive functions of AD rats through shortening the escape latency,increasing the number across the platform,platform quadrant dwell time and the percentage of the total distance run platform quadrant.The protein expression of AKT was significantly up-regulated and that of GSK3β and Tau were decreased remarkably in the hippocampal CA1 area.CONCLUSION Our study is the first to show that LSS significantly improve the cognitive function and prevent hippocampal neuronal injury of the rats with AD by activation of the PI3K/AKT/GSK3βsignaling pathway,suggesting LSS may be developed into the nutrient supplement for the treatment of AD.
基金Supported by Superior and Characteristic Specialty Construction Project of Guangxi’s Colleges and Universities(201452)
文摘The aim was to optimize ultrasonic extraction conditions and study antioxidant activities of lychee pericarp polysaccharides. The effects of extraction temperature,time,ultrasonic power,solid-liquid ratio on polysaccharides yield were investigated by single-factor experiment and orthogonal experiment to obtain optimum extraction conditions; the antioxidant activities of lychee pericarp polysaccharides were evaluated by the total antioxidant activity. Optimum extraction process was under extraction temperature of 80 ℃,extraction time of 2. 5 h,ultrasonic power of 180 W,solid-liquid ratio of 1∶ 25 g / m L; the total antioxidant activity was increased with the increasing concentration of polysaccharides. The extraction process is simple and efficient. The extraction rate of lychee pericarp polysaccharides with antioxidant activities is high.
基金Supported by Ministry of Education Project of Integrating Production, Teaching and Research in Guangdong Province(2009B09030332)Science and Technology Plan Project in Guangdong Province (2010A020506022)Special Public Welfare Industry(Agriculture) Scientific Research Foundation of Ministry of Agriculture(201103003)
文摘Through field experiment,we explore the impact of long-term drip fertigation on growth and distribution of lychee root and changes of soil pH in different layers of soil in lychee garden.The results show that drip fertigation can significantly promote the growth of lychee roots,and increase the contact area of root and soil;if it experiences six years of drip fertigation successively,the dry weight of root,root length and surface area of root in soil in drip fertigation area,will be 2.29 times,2.17 times and 2.25 times that in non-drip fertigation area,respectively.The lychee root is mainly distributed in 0-40 cm layer of soil,but there is conspicuous difference between drip fertigation area and non-drip fertigation area in terms of root distribution in 0-20 cm and 20-40 cm layer of soil.Drip fertigation is more favorable for the root to go deep inside the soil.Under long-term drip fertigation,the soil acidification in lychee garden is prominent,and in comparison with non-drip fertigation area,there is the greatest decline in soil pH in 10-20 cm layer of soil in drip fertigation area,reaching 1.47 units.
文摘Background Lychee seeds were fermented by three kinds of bacteria(Lactobacillus plantarum,Saccharomyces cer-evillus and ganoderma lucidum mycelium),and two effective strains were selected by two indexes of activity content and antioxidant,so as to further verify whether lychee seeds have waste multiplication effect and can protect cells damaged by oxidation from anti-inflammatory,anti-aging and safety.Results The contents of polyphenols,flavonoids and proteins in the solution fermented by Ganoderma lucidum mycelium did not increase,thus affecting the antioxidant capacity of the solution was far less than that of the water extract.The active content of the other two fermentation solutions was higher than that of the water extract,and the ability of scavenging free radicals of the two solutions increased with the increase of the volume fraction.At the cellular level,the two fermentation solutions showed repair effects on UVA-induced damaged cells.The contents of type I collagen(COL-1),total antioxidant capacity and ELN were increased,the contents of reactive oxygen species and MDA were decreased,and the expressions of inflammatory factors IL-6,TNF-a,iNOS and COX-2 were decreased in HaCaT cells.From the gene level,the mRNA contents of IL-6,TNF-a,Caspase-3,Caspase-9,Bax and Bcl were significantly decreased.The test of chick embryo chorioallantoic membrane(HTET CAM)showed that there was no bleeding and litchi seed fermentation liquid was not irritating.Conclusions Therefore,two kinds of litchi seed fermentation can be used as natural plant raw materials for cosmetics,and have strong antioxidant,anti-inflammatory and anti-aging functions on skin,and also have good human safety.
文摘Juice drinks are an important commercialization alternative for lychee, a tropical and subtropical fruit. Although the lychee juice content is important when assessing the quality of a drink, there are no published methods to determine it, particularly simple ones for the routine inspection of juice drinks. Lychee juice drinks contain ingredients with buffering capacity including proteins and ions such as phosphate, citrate, lactate, carbonate, acetate and propionate. The relationship between their buffering capacity and lychee juice content was studied. Citric acid was added to pure lychee dilutions in distilled water containing 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20% and 10% lychee juice. The pH of the dilutions was measured to obtain a linear model for the molar H+ concentration as a function of the added citric acid (g/L) amount LC = (BC-494.2)/12,031, where LC was the lychee juice content and BC was defined as the juice buffering coefficient.
基金Project supported by grants from the National Natural Science Foundation of China and International Board for Plant Genetic Resources.
文摘Roberts has divided seeds into orthodox seeds and recalcitrant seeds according to their storage behaviours. The former can tolerate desiccation and freezing temperatures, whereas the latter are susceptible to the two factors. When their water contents are lower than cer-
基金the National Natural Science Foundation of China(No.31201399)Shenzhen Technology Innovation Program(No.JCYJ20140508155916427)Program for Science&Technology Innovation Talents in Universities of Henan Province(No.14HASTIT023)for the financial support of materials and the equipment.
文摘Micrographs of lychee pericarp and pulp during microwave vacuum drying were tested and analyzed in order to illuminate the microstructure change of lychee and effect of the change on moisture removing in lychee.The results showed that the pericarp consisted of three parts:outer layer with cuticle,inter layer and inner layer.Outer layer and inter layer cells are easily destroyed than inner layer because of small and intact inner layer cells.Furthermore,micrographs showed that the moisture content of pulp keep constant with the temperature increasing at first 40 min due to the inner layer cells prevent the moisture removing from pulp.The long tubular structure of pulp cell would become break and lost over time,because the intercellular spaces reduced and the moisture removing was slow down in pulp.Meanwhile,the microstructure of lychee dried with temperature control was better than that without temperature control.
基金Project supported by the National Natural Science Foundation of China and the International Board for Plant Genetic Resources.
文摘Lychee is one of the famous fruits in the subtropical regions. However, lychee seedsdo not undergo maturation desiccation during development, and cannot tolerate drying andchilling in storage. Under natural conditions, lychee seeds can only maintain viability forseveral days, and their germplasm conservation is rather difficult using conventional meth-ods. So they are called recalcitrant seeds.
基金the National Natural Science Foundation of China(Grant No.31260398)the Hainan Province Natural Science Foundation,P.R.China(Grant No.313044).
文摘Acetic acid is the main component of the volatile acid in the wine.However,excessive amounts of acetic acid negatively affect wine quality.The study aimed to decrease acetic acid content produced by Saccharomyces cerevisiae fermentation after adding metal ion at different temperatures.Response surface methodology(RSM)was used to predict the optimum conditions for acetic acid removal.A central composite design was employed for the experiments and results were analyzed to obtain the best possible combination of fermentation temperature(X1:16℃-24℃)and concentrations of potassium(X2:0-12.0 mM),magnesium(X3:0-8.0 mM),and calcium ions(X4:0-0.2 mM)that would generate the minimum acetic acid in lychee wine at an initial acetic acid concentration of 1.5 g/L.Experimental data were fitted to a second-order polynomial equation using multiple regression analysis and analyzed using analysis of variance(ANOVA).During fermentation under pre-established conditions,the correlation coefficients R2 and Adj-R2 of the models for acetic acid removal were 0.9487 and 0.9007,respectively.After testing,the optimum conditions for acetic acid removal were determined as follows:fermentation temperature of 20℃;potassium,magnesium,and calcium ion concentrations of 10.1 mM,6.1 mM,and 0.2 mM,respectively.The experimental acetic acid content of lychee wine under optimal conditions was found to be 0.309 g/L,which agreed well with the model-predicted value of 0.314 g/L.
基金The project funded by Guangdong Province’s key research and development program(2020B0202080003),ChinaGuangzhou science and technology project(201903010051,202103000055),China+1 种基金the Guangdong special support program(2019BT02N112),Chinathe Special fund for scientific innovation strategy construction of high level Academy of Agriculture Science(R2017YJYB2006,R2020PY-JG011,202108TD),China.
文摘Lychee pulp phenolic extract (LPPE) possessed protective effects against nonalcoholic fatty liver disease (NAFLD), while the specific components concerned were unclear. In this study, twelve major components in LPPE were identified by LC-MS and prepared by on line knockout methods. Two antioxidant methods companied with hepatocyte steatosis assay were used to evaluate the contribution of twelve components to the hepatoprotective effects of LPPE. The identified components include four procyanidins and eight flavonoids, they all play active roles in the oxygen radical absorbance capacity of LPPE, while only B-type procyanidin trimer and quercetin 3-O -rutinoside-7-O -rhamnoside exert positive effects on cellular antioxidant activity of LPPE. Six components including procyanidin B2, epicatechin, two quercetin glycosides, and two procyanidin trimers are considered as contributors to lipid accumulation inhibitory effects of LPPE with B-type procyanidin trimer being identified as the main contributor. All the twelve components were recognized as hepatoprotective components against NAFLD in LPPE.
文摘In a bustling square in Guangzhou’s southern Haizhu district,a group of young activists are performing a play about a local farmer’s struggle after a failed lychee harvest.Nearby,an art exhibit documents how the sale of lychees in local markets,once a common sight,is becoming increasingly rare.Both events unfolded at the Canton Harvest Festival organized by local NGOs specializing in urban-rural development on December 1,2024.
文摘The timing is almost poetic.As lychees ripened in orchards this season,the online drama Lychee in Chang'an fickered onto screens-reawakening an ancient tale of obsession.The series resurrects Tang Dynasty(618-907)couriers who thundered down imperial roads day and night,racing against time to deliver Guangdong's precious tropical fruit to the emperor's consort in Xi'an.For centuries。
基金part of an international program named“Sustainable Land Use and Rural Development in the Mountainous Regions of Southeast Asia”(SFB 564)funded by Deutsche Forschungsgemeinschaft(DFG)+1 种基金the National Research Council of Thailand(NRCT)financial support from the Eiselen Foundation Ulm and Deutscher Akademischer Austausch Dienst(DAAD).
文摘The litchi is a fruit essential for the economies of several Southeast Asian countries,but markets regularly reject it,mainly due to spoilage.Drying extends the shelf-life of litchis,but in Thailand the optimal characteristics of the dried product have not yet been determined.The purpose of this study was to determine the optimum physicochemical properties of dried litchis-those suitable for Thai consumers.The dried fruits were submitted to physicochemical measurement and consumer evaluation,with datasets subsequently integrated using circular ideal-point regression analysis.Response surface methodology was then used to predict the optimum physicochemical properties of the fruits.It was found that Thai consumer preferences with regard to dried litchis are for the fruits to be of golden-yellow color(L*ranging around 54;H ranging around 79°),to have a soft flesh(SMF ranging between 13 and 14 kN/100 g)and to have a sweet taste(TSS:TA ranging between 25 and 28).The results may be used in the future to prescribe pretreatments and drying conditions.
基金supported by funding from Science and Technology Projects in Guangzhou(Grant No.2023A04J1455)the Key-Area Research and Development Program of Guangdong Province(#2022B0202070003)supported by the National Natural Science Foundation of China(#32072547,#32372665).
文摘Many species of Sapindaceae,such as lychee,longan,and rambutan,provide nutritious and delicious fruit.Understanding the molecular genetic mechanisms that underlie the regulation of flowering is essential for securing flower and fruit productivity.Most endogenous and exogenous flowering cues are integrated into the florigen encoded by FLOWER-ING LOCUS T.However,the regulatory mechanisms of flowering remain poorly understood in Sapindaceae.Here,we identified 60 phosphatidylethanolamine-binding protein-coding genes from six Sapindaceae plants.Gene duplication events led to the emergence of two or more paralogs of the FT gene that have evolved antagonistic functions in Sapindaceae.Among them,the FT1-like genes are functionally conserved and promote flowering,while the FT2-like genes likely serve as repressors that delay flowering.Importantly,we show here that the natural variation at nucleotide position-1437 of the lychee FT1 promoter determined the binding affinity of the SVP protein(LcSVP9),which was a negative regulator of flowering,resulting in the differential expression of LcFT1,which in turn affected flowering time in lychee.This finding provides a potential molecular marker for breeding lychee.Taken together,our results reveal some crucial aspects of FT gene family genetics that underlie the regulation of flowering in Sapindaceae.
